Non-Invasive Imaging of Metabolic Fluxes and Enzymatic Activity in Living Mice

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1 on-invasive Imaging of Metabolic Fluxes and Enzymatic Activity in Living Mice Hacer KARATA, Ph.D. Postdoctoral cientist DUBIKVKAYA LAB Laboratory of Bioorganic Chemistry and Molecular Imaging (LCBIM) June-6-14

2 Laboratory of Bioorganic Chemistry and Molecular Imaging (LCBIM) Development of Probes and Methods for in vitro and in vivo Imaging A novel approach for imaging metabolic fluxes in vivo A novel approach for imaging of proteases in vivo

3 Bioluminescent imaging (BLI) atural process based on the oxidation of D-luciferin by Luciferase on-invasive detection method High sensitivity mol/l 100,000 times more sensitive than fluorescence High specificity Low background

4 Applications of BLI to probe molecular signatures of target tissues CAGE X C 2 H UCAGIG HX C 2 H Firefly luciferase + H X =, H CAGE Free Luciferin ATP, 2, AMP, PP i, Mg 2+ C 2 xyluciferin (1) at Methods, 2006, 3, 295. (2) Mol Ther, 2005, 11, 926. (3) Proc. atl. Acad. ci. 2010, (4) Angew. Chem. Int. Ed. 2007, 46, 7031.

5 A novel approach for imaging metabolic fluxes in vivo with Prof. Andreas tahl Group utritional cience and Toxicology Department, UC Berkeley, CA

6 Uptake of fatty acids into cells H Fatty Acid Transporter Proteins (FATP) Cytosol FFA uptake regulated by FATP FATP linked to obesity Pflugers Arch. (2004) 447, 722

7 Blocking FATP in the intestine reduces caloric uptake H X = Decreased Fatty Acid uptake Cytosol FATP promising therapeutic target to control FFA uptake

8 Activable bioluminescent fatty acid probe FFA (LIPID) Releasable Linker Luciferin (IMAGIG PRBE) CCD Camera H C 2 H FATP FATP FFA--Luc Cytosol H C 2 H Glutathione (GH) H H H C 2 H H C 2 H Luciferese Mg 2+, ATP + oxyluciferin

9 Intestinal fatty acid uptake in real time in live animals Time course after oral gavage AC Chem Biol, 2012, 7, 1884

10 Intestinal fatty acid uptake in real time in live animals AC Chem Biol, 2012, 7, 1884

11 MicroCT and BLI overlay of uptake Ba 4 radiocontrast agent for intestine labeling AC Chem Biol, 2012, 7, 1884

12 Where does the light come from? The duodenum is the first section of the small intestine tomach Duodenum Colon AC Chem Biol, 2012, 7, 1884

13 Comparison to the control compound H C 2 H H FFA (LIPID) Luciferin (IMAGIG PRBE) Releasable Linker C 2 H 60 min 60 min

14 A novel approach for imaging of proteases in vivo Godinat et. al, AC Chem. Biol., 2013, 8 (5),

15 plit Luciferin Approach Godinat et. al, AC Chem. Biol., 2013, 8 (5),

16 plit Luciferin Approach; Advantages Higher permeability Easier chemistry imultaneous detection Better stability Lower cost

17 Does this reaction work in vivo? IP injection FVB-luc+ mice Dose : mmol/kg In collaboration with Doug Hanahan 15 min post-injection Godinat, A. et al, AC Chem. Biol., 2013, 8 (5), pp

18 Caspase-3 activity imaging in vitro Godinat, A. et al, AC Chem. Biol., 2013, 8 (5), pp

19 Caspase-3 activity imaging in vitro Godinat, A. et al, AC Chem. Biol., 2013, 8 (5), pp

20 Caspase-3 activity imaging in FVB+luc Mice

21 Caspase-3 activity imaging in FVB+luc Mice 3 fold 6 fold $60/mouse/inj. $2/mouse/inj.

22 Conclusions Fatty Acid Transport Protein functions Protease activity Copyright: millville.sps.edu

23 Development of new probes Image guided surgery Mitochondria membrane potential Activity of Glucose Transporters Tumor Imaging Copyright: micro.magnet.fsu.edu; wikimedia.org/wikipedia; nature medicine

24 Acknowledgements Prof. Elena A. Dubikovskaya Laboratory of Bioorganic Chemistry and Molecular Imaging Aurélien GDIAT, M.. Matthew Boygo, Ph.D Gennady ikitin, M.. Grigory KARATEEV, M.. Ghyslain BUDI, PhD Riccardo III, PhD Manuel DREFAHL, M. Prof. Andreas tahl and Hyo Min Park (UC Berkeley, California) Prof. Douglas Hanahan and Dr. Ke Cheng (CM, EPFL, witzerland) Prof. Matthew Bogyo and Laura E. anman (tanford University, California) 24

25 Laboratory of Bioorganic Chemistry and Imaging beyond the Molecular Imaging (LCBIM) Proteome Elena Dubikovskaya 12/15/10