Integrated Biological Control of Woolly Apple Aphid

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1 Integrted Biologicl Control of Woolly Apple Aphid Apple Crop Protection Reserch Review Ykim Convention Center, Ykim, WA 27 Jnury, 211 2:45 pm Elizeth H. Beers WSU Tree Fruit Reserch & Extension Center 11 N. Western Ave. Wentchee, Wshington

2 3-yer project (28-21) Identify nd quntify the predtors nd prsitoids of woolly pple phid in estern Wshington (yer 1). Determine the reltive impct of the predtors nd prsitoids (yers 2 nd 3). Determine the effect of orchrd pesticides on the key nturl enemies (yers 2 nd 3).

3 Oj. 1: ID, quntify nturl enemies Other, 4.8% Lcewings, 23.6% *Aphelinus mli only prsitoid found Ldy Beetles, 8.9% Syrphids, 62.7% Other, 3.% Lcewings, 6.% Lcewings, 12.% 28 Ldy Beetles, 13.% Ldy Beetles, 24.% Syrphids, 62.% Syrphids, 81.% 26 27

4 Colonies/5-min serch Mid-seson Oj. 1: side enefit (WAA sesonl phenology - 28) Apr My Jun Jul Aug Sep Oct Nov 12 Mid- nd Lte Seson Lte Seson Apr My Jun Jul Aug Sep Oct Nov Apr My Jun Jul Aug Sep Oct Nov

5 Oj. 2: Reltive impct of predtors nd prsitoids Mid-summer Fll WAA only WAA + A. mli WAA + A. mli + predtors Men WAA/tree 1 5 Men WAA/tree Jun 28-Jun 5-Jul 12-Jul 19-Jul 26-Jul 2-Aug c 6-Sep 13-Sep 2-Sep 27-Sep 4-Oct 11-Oct 18-Oct 25-Oct c

6 Entrust Lorsn Sevin Guthion Clypso Assil Wrrior II Cyzpyr Oj. 3: Impct of Pesticides on A. mli 31. Acute toxicity Kumulus Ultor Koc+Mn.1x 1x Corrected % Mortlity

7 3.5 Fecundity.7 Proportion Femles 3..6 Men Dily Fecundity % Mortlity Mortlity Control Proportion Femles R (Popultion Growth) R Control A. mli Sulethl Biossys 32. Sulethl effects Control. Control

8 Oj. 3: Impct of Pesticides on A. mli 32. Sulethl effects Prsitoid Aundnce (Adults) Control Time (dys)

9 Oj. 3. Pesticide effects Lrge plots Trt PF (1 DD) 32 fl oz Intrepid 16 fl oz 32 fl oz Intrepid 16 fl oz 1st cover (35 DD) 7 oz 7 oz 4.5 oz 4.5 oz 2nd cover (+17 d) 7 oz 7 oz 4.5 oz 4.5 oz 4 trts x 4 replictes Ech rep cres (Totl 16-2 cres) Smpled every other week April-Nov: WAA, GAA, mites WAA predtors/pr HIPV trps A. mli sticky crds Erwig trps Tp Counts

10 29 Lrge Plot: Bridgeport 5 91 WAA colonies/5 min Intrepid- - Intrepid- Intrepid- Woolly Apple Aphid d - My Jun Jul Aug Sep Oct Nov Dec Intrepid- c WAA colonies (sesonl men)

11 29 Lrge Plot: Bridgeport 25 2 Erwigs Erwigs/trp Intrepid- - Jun Jul Aug Sep Oct Nov 91 - Intrepid- - Intrepid- Intrepid Erwigs/trp (sesonl sum)

12 29 Lrge Plot: Othello WAA colonies/5 min Intrepid- - Intrepid- Intrepid- Woolly Apple Aphid c - c My Jun Jul Aug Sep Oct Nov Dec Intrepid WAA colonies (sesonl men)

13 21 Lrge Plot: Othello WAA colonies/5 min Intrepid- - Intrepid- Apr My Jun Jul Aug Sep Oct Nov Dec Intrepid- - Intrepid- Woolly Apple Aphid 29 c 21 c c WAA colonies (sesonl men)

14 Trt Oj. 3. Pesticide effects Lrge plots protocol #2 PF (1 DD) Intrepid 16 fl oz Intrepid 16 fl oz Intrepid 16 fl oz Intrepid 16 fl oz 1st cover (35 DD) 7 oz 4.5 oz Wrrior 2.56 fl oz Guthion 2 l 2nd cover (+17 d) 7 oz 4.5 oz Wrrior 2.56 fl oz Guthion 2 l 4 trts x 4 replictes Ech rep 1 cres (Totl 16 cres) Smpled every other week April-Nov: WAA, GAA, mites WAA predtors/pr HIPV trps A. mli sticky crds Erwig trps Tp Counts

15 21 Lrge Plot: Othello # WAA colonies/5 min Wrrior II Guthion Guthion Woolly Apple Aphid Wrrior II c Apr My Jun Jul Aug Sep Oct Nov Dec WAA colonies (sesonl men)

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