Assessment of Attracticidal Spheres as a Tool for Management of Spotted Wing Drosophila
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1 Assessment of Attrcticidl Spheres s Tool for Mngement of Spotted Wing Drosophil Leskey Lortory USDA ARS Applchin Fruit Reserch Sttion Kerneysville, WV 25430
2 Spotted Wing Drosophil in Mid-Atlntic Common cultivted hosts ttcked: Cherry, pech, rsperry, lckerry, lueerry, strwerry Mngement chllenges Monitoring trps re not lwys relile Rpid development with numerous genertions Oviposition cn occur t hrvest Current mngement options Chemicl control triggered y reltive mturity of crop Snittion Aggressive hrvesting Netting
3 Visully integrted spheres mximize vilility nd lethlity of toxicnt. Do ttrcticidl spheres designed for AMF hold promise for SWD?
4 Evlute lethlity of ttrcticidl spheres with known toxicnts for SWD Ojectives Determine if SWD dults will orient to nd light on ttrcticidl spheres deployed under semi-field conditions
5 Evlution of Lethlity Insecticides: Bifenthrin, Lmdcyhlothrin, Spinetorm, nd Spinosd. Rtes: 0.0, 0.01, 0.1, 0.5 nd 1.0%.i. Min. 20 mles nd 20 femles/insecticide/rte. Exposure period: 5 min. Evlution of toxic effects t 0, 24 nd 48 h fter exposure
6 Forging Time (s) Forging Time(s) of Adult D. suzukii y Rte of Active Ingredient c c c Bifenthrin λ-cyhlothrin Spinetorm Spinosd Active Ingredient
7 Percent Mortlity Percent Mortlity of Adult D. suzukii 48 h After 5-min Exposure to Insecticides c c c c d d c c 0.0 Bifenthrin λ-cyhlothrin Spinetorm Spinosd Active Ingredient
8 Alightment nd Orienttion to Spheres nd Attrctnt Five Tretments Sticky sphere Sticky sphere + Attrctnt Sticky sphere + Rsperry plnt Sticky sphere + Attrctnt + Rsperry plnt Rsperry plnt Monterey Insect Bit, Monterey AgResources. 25 Mles nd 25 Femles per tretment Mintined on Formul 4-24 Instnt Drosophil Medium (Crolin Biologicl Supply). Strved 24 h with wter nd 7-10-d old t time of relese. Flies llowed to forge freely for 48 h. Recorded numer of SWD cptured on spheres nd numer recovered from rsperry fruit
9 Semi-Field Deployment
10 Men # SWD Adults 25 Men Numer of SWD Cptured on Sticky Spheres y Tretment Sphere Sphere + Attrctnt Rsperry + Sphere Rsperry + Sphere + Attrctnt Tretment
11 Lrvl/Pupl Infesttions
12 Men # SWD/Fruit Men Numer of SWD/Rsperry in ech Tretment Rsperry Rsperry + Sphere Rsperry + Sphere + Attrctnt Tretment
13 Preliminry Results Lethlity Trils 1. λ-cyhlothrin (Kiso WG) very effective down to 0.1%.i., even with reduced feeding times. 2. Other insecticides t 1.0 nd 0.5% look promising.
14 Preliminry Results Semi-Field Trils 1. No pprent effect of ttrctnt in cptures on spheres or SWD recovered from fruit 2. Presence of rsperry plnt in cges reduced sticky sphere cptures ~50% 3. Presence of sticky spheres reduced SWD infesttion in fruit y ~50%
15 Future Reserch Identify ttrctive, optiml visul stimuli nd incorporte into trp design. Size, shpe, nd color Orienttion nd lightment within susceptile crop Physiologicl stte of responders nd non-responders. Incorporte novel ttrctnts nd evlute under semifield nd field conditions
16 Acknowledgments North Americn Rsperry nd Blckerry Assocition Driscoll Strwerry Assocites, Inc. Monterey AgResources Dow AgroSciences, LLC Nufrm Americs Brittny Rnkin Sen Wiles Preston Brown, OSU
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