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1 !"#$%!&%##&'()*+,-.&/01*-0)*,(&%,2345() Date of Submission Name: Lab Antibody Name: Target: Company/ Source: Catalog Number, database ID, laboratory Lot Number Antibody Description: Target Description: Species Target Species Host Validation Method #1 Validation Method #2 Purification Method Polyclonal/ Monoclonal Vendor URL: Reference (PI/ Publication Information) Please complete the following for antibodies to histone modifications: if your specifications are not listed in the drop-down box, please write-in the appropriate information Histone Name AA modified AA Position Modification

2 Validation #1 Analysis Insert Validation Image (click here)

3 Immunoblot analysis of extracts from cell lines K562 (le8) and HeLaS3 (right) using MMS 126R. Pol II IP, 4ug Pol II IP, 2ug Pol II IP, 1ug IgG, 4ug IgG, 2ug IgG, 1ug MW (kd) Supernatant, 4ug MW (kd) Supernatant, 2ug B. Supernatant, 1ug A. Nuclear Extract Immunoblot/Immunoprecipitation Antibody: RNA Polymerase II Source: Covance MMS-126R Epitope: The antibody 8WG16 was developed using RNA polymerase (RNA pol II) purified from wheat germ extract. It recognizes the C-terminal heptapeptide repeat present on the largest subunit of pol II GM12878 nuclear extract was immunoprecipitated with 1,2 and 4ug Pol II annbody (Covance, MMS 126R) or normal mouse IgG as a neganve control. Supernatant was checked to see the complenon of IP. Arrow indicates band specifically immunoprecipitated by MMS 126R

4 Validation #2 Analysis Insert Validation Image (Click here)

5 Peptide count IgG control Rep1 Peptide count IgG control Rep2 Peptide count MMS126R Rep1 Peptide count MMS126R Rep2 Protein name POLR2A DNA-directed RNA polymerase II subunit RPB POLR2B DNA-directed RNA polymerase II subunit RPB RPAP2 Isoform 1 of RNA polymerase II-associated protein POLR2G DNA-directed RNA polymerase II subunit RPB POLR2H DNA-directed RNA polymerases I, II, and III subunit RPABC POLR2C DNA-directed RNA polymerase II subunit RPB POLR2J RPB11a protein POLR2E DNA-directed RNA polymerases I, II, and III subunit RPABC POLR2I DNA-directed RNA polymerase II subunit RPB ALMS1 ALMS POLR2D DNA-directed RNA polymerase II subunit RPB GRINL1A;Gcom1 Isoform 12 of Protein GRINL1A RECQL5 Isoform Beta of ATP-dependent DNA helicase Q POLR2L DNA-directed RNA polymerases I, II, and III subunit RPABC KRT6A Keratin, type II cytoskeletal 6A POLR2K DNA-directed RNA polymerases I, II, and III subunit RPABC RFC4 Replication factor C subunit RFC2 Isoform 1 of Replication factor C subunit GAN Gigaxonin POLR3E Isoform 3 of DNA-directed RNA polymerase III subunit RPC HIST1H1D Histone H RPL36 60S ribosomal protein L RPL31 60S ribosomal protein L PLEC1 Isoform 1 of Plectin TUBB2C Tubulin beta-2c chain GPSN2 Isoform 1 of Synaptic glycoprotein SC HNRPUL1 Isoform 1 of Heterogeneous nuclear ribonucleoprotein U-like protein FLG2 Ifapsoriasin RPS26 Novel protein similar to ribosomal protein 26 RPS Similar to ribosomal protein S RPS23 40S ribosomal protein S PRKDC Isoform 1 of DNA-dependent protein kinase catalytic subunit MSH3 DNA mismatch repair protein Msh APOBEC3B Isoform 1 of Probable DNA dc->du-editing enzyme APOBEC-3B RPL27 60S ribosomal protein L IGKC Immunoblobulin light chain (Fragment) SLC25A3 Isoform A of Phosphate carrier protein, mitochondrial precursor

6 CETN2 Centrin BANF1 Barrier-to-autointegration factor RPS20 40S ribosomal protein S RPL28 60S ribosomal protein L HIST1H2AH Histone H2A type 1-H RPL18 60S ribosomal protein L RALY RNA binding protein (Fragment) RPL26L1 60S ribosomal protein L26-like TFG Protein TFG ATP5A1 ATP synthase subunit alpha, mitochondrial precursor EWSR1 cdna FLJ31747 fis, clone NT2RI , highly similar to RNA- BINDING PROTEIN EWS PDCD6 Programmed cell death protein RPS10 40S ribosomal protein S RPL37A 60S ribosomal protein L37a TCEB2 Transcription elongation factor B polypeptide RPL6 60S ribosomal protein L HNRPDL Isoform 1 of Heterogeneous nuclear ribonucleoprotein D-like RBMS1 Isoform 2 of RNA-binding motif, single-stranded-interacting protein KRT18 Keratin, type I cytoskeletal PCBP1 Poly(rC)-binding protein RPL30 60S ribosomal protein L RPL11 Isoform 1 of 60S ribosomal protein L RPLP1 60S acidic ribosomal protein P RPS5 40S ribosomal protein S RPL23A;hCG_ S ribosomal protein L23a FAU ubiquitin-like protein fubi and ribosomal protein S30 precursor HNRNPA2B1 HNRPA2B1 protein DDX3X ATP-dependent RNA helicase DDX3X RPL35 60S ribosomal protein L SUB1 Activated RNA polymerase II transcriptional coactivator p PHGDH D-3-phosphoglycerate dehydrogenase RPS25 40S ribosomal protein S EEF1A2 Elongation factor 1-alpha GAPDH Glyceraldehyde-3-phosphate dehydrogenase kda protein PCMT1 Isoform 1 of Protein-L-isoaspartate(D-aspartate) O-methyltransferase RPS11 40S ribosomal protein S RPL8 60S ribosomal protein L RPS24 Isoform 1 of 40S ribosomal protein S RPL13 60S ribosomal protein L

7 CPS1 Isoform 1 of Carbamoyl-phosphate synthase [ammonia], mitochondrial precursor TTN Isoform 2 of Titin RPL24 60S ribosomal protein L TIAL1 Nucleolysin TIAR HNRNPA1 Isoform A1-B of Heterogeneous nuclear ribonucleoprotein A RBMS2 RNA-binding motif, single-stranded-interacting protein LOC similar to QM protein isoform KRT75 Keratin, type II cytoskeletal RPL38 60S ribosomal protein L RPL7A 60S ribosomal protein L7a RPL18A 60S ribosomal protein L18a RPS16 40S ribosomal protein S TREX1;ATRIP Isoform 3 of Three prime repair exonuclease hcg_21078 hypothetical protein LOC RBM14 Isoform 1 of RNA-binding protein APOBEC3C;APOBEC3D Probable DNA dc->du-editing enzyme APOBEC-3C GRPEL1 GrpE protein homolog 1, mitochondrial precursor RPLP0 60S acidic ribosomal protein P RPL23 60S ribosomal protein L HIST2H2BE Histone H2B type 2-E RPS19 40S ribosomal protein S RPS2 40S ribosomal protein S RPL7 60S ribosomal protein L Anti-colorectal carcinoma heavy chain KRT16 Keratin, type I cytoskeletal CEBPB CCAAT/enhancer-binding protein beta RPL12 60S ribosomal protein L RPLP2 60S acidic ribosomal protein P HNRPH1 Heterogeneous nuclear ribonucleoprotein H RPS8 40S ribosomal protein S RPS13 40S ribosomal protein S MSH2 DNA mismatch repair protein Msh RPS6 40S ribosomal protein S RAD23B UV excision repair protein RAD23 homolog B TOP3A Isoform Long of DNA topoisomerase 3-alpha PCBP2 poly(rc)-binding protein 2 isoform b HNRNPU Isoform Short of Heterogeneous nuclear ribonucleoprotein U LOC similar to RPL13 protein HLTF Isoform 1 of Helicase-like transcription factor RPS3 40S ribosomal protein S

8 FUS Isoform Short of RNA-binding protein FUS IGKV4-1 Similar to Ig kappa chain V-IV region precursor HIST1H4E;HIST1H4A;HIST2H4B;HIST1H4L;HIST1H4F;HIST1H4C;HIST2H4A;H IST1H4I;HIST1H4D;HIST4H4;HIST1H4K;HIST1H4J;HIST1H4B;HIST1H4H Histone H RPL4 60S ribosomal protein L GRN Isoform 1 of Granulins precursor HSPA8 Isoform 1 of Heat shock cognate 71 kda protein KRT14 Keratin, type I cytoskeletal WDR76 WD repeat-containing protein PARP1 Poly [ADP-ribose] polymerase HNRNPC Isoform C1 of Heterogeneous nuclear ribonucleoproteins C1/C XPC DNA-repair protein complementing XP-C cells RPS9 40S ribosomal protein S HNRPK Isoform 1 of Heterogeneous nuclear ribonucleoprotein K SLC25A5 ADP/ATP translocase IMPDH2 Inosine-5'-monophosphate dehydrogenase RPS18;LOC S ribosomal protein S TUBA1B Tubulin alpha-1b chain XRCC5 ATP-dependent DNA helicase 2 subunit HNRNPA2B1 Isoform B1 of Heterogeneous nuclear ribonucleoproteins A2/B PURA Transcriptional activator protein Pur-alpha HSPA1A;HSPA1B Heat shock 70 kda protein HSPA5 HSPA5 protein XRCC6 ATP-dependent DNA helicase 2 subunit HNRPAB Isoform 4 of Heterogeneous nuclear ribonucleoprotein A/B KRT5 Keratin, type II cytoskeletal TUBB Tubulin beta chain RPS4X 40S ribosomal protein S4, X isoform ALB Uncharacterized protein ALB PURB Transcriptional activator protein Pur-beta HNRPD Isoform 1 of Heterogeneous nuclear ribonucleoprotein D CSDA Isoform 1 of DNA-binding protein A RPA3 Replication protein A 14 kda subunit RPA2 Isoform 1 of Replication protein A 32 kda subunit KRT9 Keratin, type I cytoskeletal SSBP1 Single-stranded DNA-binding protein, mitochondrial precursor YBX1 Nuclease-sensitive element-binding protein KRT2 Keratin, type II cytoskeletal 2 epidermal KRT10 Keratin, type I cytoskeletal KRT1 Keratin, type II cytoskeletal

9 RPA1 Replication protein A 70 kda DNA-binding subunit HSPA9 Stress-70 protein, mitochondrial precursor