Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Cellular Pathology Laboratory Contact: Breege Nicholson Queen Elizabeth Hospital Tel: +44 (0) ext Stadium Road Woolwich Website: SE18 4QH Testing performed at the above address only Laboratory locations: Locations covered by the organisation and their relevant activities Location details Activity Address Cellular Pathology Laboratory Queen Elizabeth Hospital Stadium Road Woolwich SE18 4QH Local contact Breege Nicholson Tel: +44 (0) ext E mail: breege.nicholson@nhs.net Routine Histopathology Special Stains Immunohistochemistry Site activities performed away from the locations listed above: Location details Activity On site body store Queen Elizabeth Hospital Stadium Road Woolwich SE18 4QH Mortuary Services (body receipt, storage and release) Assessment Manager: VB Page 1 of 9

2 DETAIL OF ACCREDITATION HUMAN BODY TISSUE Tissue samples (including breast; gastrointestinal; skin; urological; bronchial/lung/thoracic; endocrine including thyroid, parathyroid, adrenal; lymphoreticular including lymph nodes, spleen, and bone marrow trephines [processing only for bone marrow trephines]; liver; bone and soft tissues;, gynaecological; and head and neck) Formalin Fixed Tissue Tissue (FFPE) diagnosis Examination of tissues in order to identify or exclude morphological and cytological abnormalities for the purpose of diagnosis Supported by: Specimen dissection: Cut-up Manual - Routine Specimens categorised according to the RCPath as, SOP CHQ-DP-008 & SOP CHQ- DP-009, CHQ-RP & CHQ DP 0010 A specimens B specimens C specimens D specimens Decalcification using acid decalcifiers (Decalcifier 1 and 2) SOP CHQ-DP-0010 Processing using Sakura TissueTek VIPs ; VIP5 : SOP CHQ-MP-0026 VIP6 : SOP CHQ-MP-0065 Embedding using : Tissue tek Embedding Centre SOP CHQ-MP- 005 Microtomy: Using rotary Microtomes, Shandon Finesse, Leica (SOP CHQ-MP-006, CHQ- MF-0024) Macroscopic and Microscopic examination: Documented in-house procedures for staining for staining and reading Assessment Manager: VB Page 2 of 9

3 slides DEMONSTRATION OF: Basophilic and eosinophilic structures Acid Mucopolysaccharides Acid and Neutral Mucopolysaccharides Amyloid Elastic fibres and connective tissue Bile pigments Fungi including Pneumocystis jiroveci (carinii) Mucin; cryptococcus Helicobacter/Microorganisms Gram +ve & -ve microogranisms Connective tissue and fibrin Melanin, Argentaffin and Lipofuchsin Connective tissue Haematoxylin and Eosin staining SOP CHQ-SP-001 in conjunction with manufacturer s instructions for Leica Autostainer Special Tinctorial Histochemical Staining: Alcian Blue (SOP CHQ-SP-003) Alcian Blue/Periodic Acid Schiff (SOP CHQ-SP-002) Congo Red (SOP CHQ-SP-005) Elastic/Haematoxylin Van Gieson (EVG/HVG) (SOP CHQ-SP-007, CHQ-SP-014) Fouchet van Gieson (SOP CHQ- SP-008) Grocott Hexamine Silver (SOP- CHQ-SP-013) Mucicarmine (SOP CHQ-SP-0020) Giemsa (SOP CHQ-SP-009) Gram (SOP CHQ-SP-0011) Martius Yellow, Brilliant Crystal Scarlet, Soluble Blue (MSB) (SOP CHQ-SP-0019) Masson Fontana (SOP CHQ-SP- 0016) Masson s Trichrome (SOP CHQ- SP-0017) Assessment Manager: VB Page 3 of 9

4 Neutral Mucopolysaccharides, glycogen and fungus Haemosiderin and ferric iron Reticulin fibres Copper-associated protein and Hepatitis B-affected Mast Tubercle bacilli Melanin bleach Leprosy bacilli Spirochaetes Special Tinctorial Histochemical Staining: (cont d) Periodic Acid Schiff (PAS) & Diastase PAS (D/PAS) (SOP CHQ- SP-0023) Perl s Prussian Blue (SOP CHQ- SP-0024) Reticulin- Gordon & Sweets (SOP CHQ-SP-0010) Shikata s Orcein (SOP CHQ-SP- 0022) Toluidine Blue (SOP CHQ-SP- 0028) Ziehl Neelsen (ZN) (SOP CHQ-SP- 0021) Mallory Melanin Bleach (SOP CHQ- SP-0015) Wade-Fite (SOP CHQ-SP-0029) Warthin Starry (SOP CHQ-SP- 0030) Assessment Manager: VB Page 4 of 9

5 following: Embryonic cell in normal tissues and germ cell tumours Cytokeratins (acidic, basic) HGPIN, prostate adenocarcinoma T lymphocytes, follicular lymphoma, basal cell carcinoma Epithelial cell types Pancreaticobiliary adenocarcinomas antibodies: Alpha Fetoprotein AE1/AE3 AMACR (Racemase) Bcl2 oncoprotein Ber Ep4 CA 19.9 Ovarian tumours CA 125 Smooth muscle Myoepithelial, smooth muscle Mesothelial Caldesmon Calponin Calretinin Glandular CAM 5.2 T Mature T, T cell lymphomas Mature B, CALLA Granulocytes, Reed-Sternberg B CD3 CD5 CD10 CD15 CD20 Assessment Manager: VB Page 5 of 9

6 following: (cont d) Reed-Sternberg, plasma, ALCL Endothelial Haematopoetic, vascular endothelium Natural killer, neuroendocrine tissues Macrophages, monocytes, myeloid B Ewing Sarcoma, atypical fibroxanthoma Mast, GIST Plasma Intestinal epithelial, colorectal + other adenocarcinomas CEA glycoproteins Tissues of neuroendocrine origin CytoMeglo Virus Mantle cell lymphoma Basal, filamentous keratins from glandular to stratified squamous antibodies: CD30 CD31 CD34 CD56 CD68 CD79alpha CD99 CD117/CKIT CD138 CDX2 CEA Chromogranin CMV Cyclin D1 Cytokeratin (HMWCK/34βE12) Assessment Manager: VB Page 6 of 9

7 following: (cont d) Epithelial tissue from glandular to stratified squamous Stratified squamous epithelium, basal, mesotheliomas antibodies Cytokeratin (MNF116) Cytokeratin 5/6 Normal and neoplastic epithelia Cytokeratin 7 Carcinomas of simple epithelium Cytokeratin 8/18 Epithelial and carcinoma Cytokeratin 19 Adenocarcinomas, columnar epithelial Striated and smooth muscle Cytokeratin 20 Desmin Lymphatic vessels D2-40 Gastrointestinal stromal tumour Normal ductal epithelial, ductal carcinoma Normal and neoplastic epithelium Endothelial cell DOG-1 E-Cadherin EMA Factor XIIIa Thyroid tumours Galectin 3 Mammary gland carcinomas Mammary gland carcinomas Mammary gland carcinomas Placental trophoblasts GATA-3 GCDFP-15 Mammaglobin HCG Assessment Manager: VB Page 7 of 9

8 following: (cont d) Hep B virus Kaposi s sarcoma Melanoma, melanocyte differentiation Sex cord stromal tumours antibodies Hep B surface antigen HHV-8 HMB45 Inhibin Proliferating Cells Ki 67 B, T, monocytes, macrophages Melanoma marker Adenocarcinoma Rhabdomyosarcoma Alveolar, lung adenocarcinoma Nuclei of expressing oestrogen High grade dysplasia of cervix Dysplasia in Barrett s oesophagus Gestational trophoblastic disease Prostate basal, myoepithelial Prostatic glandular epithelium Ovarian tumour, renal cell carcinoma LCA Melan-A MOC-31 MyoD1 Napsin A Oestrogen Receptors p16 p53 p57 p63 PAP PAX-8 Assessment Manager: VB Page 8 of 9

9 following: (cont d) Neurones, melanocytes, neuroendocrine Germ cell tumours Pneumocystis jiroveci (carinii) Nuclei of expressing Progesterone Prostate secretory and ductal epithelium Renal cell carcinoma Schwann, nerve processes, neural and melanocytic neoplasms Smooth muscle, myoepithelial Smooth muscle, myoepithelial Melanocytes Neuroendocrine Thyroid epithelial Thyroid and lung epithelial Gastrointestinal epithelium Mesothelial, serous carcinoma END antibodies PGP 9.5 PLAP Pneumocystis jiroveci (carinii) Progesterone Receptors PSA RCC S100 Smooth Muscle Actin (SMA) Smooth Muscle Myosin (SMMS-1) SOX-10 Synaptophysin Thyroglobulin TTF1 Villin WT-1 Assessment Manager: VB Page 9 of 9