Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Lister Hospital Contact: Heather Taylor Coreys Mill Lane Tel: +44 (0) Stevenage SG1 4AB Website: Testing performed at the above address only Tissue: biopsy, excision, resection, and post-mortem specimens DETAIL OF ACCREDITATION Examination of tissues in order to identify or exclude morphological and cytological abnormalities for the purpose of : HISSOP111 receipt and processing of post mortem specimens HISSOP112 receipt of POC samples Specimen Dissection using: HISSOP81 Cut up set up HISSOP78 BMS cut up HISSOP64 Assigning BMS cut up HISSOP41 Cut up of large Bladder HISSOP51 Cut up of large Prostate HISSOP60 Cut up of large Penis HISSOP37 Cut up of large Gall bladder HISSOP39 Cut up of large Parathyroid Assessment Manager: VB Page 1 of 13
2 Tissue: biopsy, excision, resection, and post-mortem specimens Examination of tissues in order to identify or exclude morphological and cytological abnormalities for the purpose of : HISSOP38 Cut up of large Thyroid HISSOP40 Cut up of large Small bowel HISSOP54 Cut up of large Uterus and cervix HISSOP42 Cut up of large Salivary gland HISSOP45 Cut up of large Ovary HISSOP46 Cut up of large Fallopian tube HISSOP44 Cut up of large Placenta HISSOP43 Cut up of large Lymph node HISSOP57 Cut up of large Vulva HISSOP55 Cut up of large Colon HISSOP49 Cut up of large laparotomy for lymphoma Assessment Manager: VB Page 2 of 13
3 Tissue: biopsy, excision, resection, and post-mortem specimens Tissue: biopsy, excision, resection, and post-mortem specimens. Cytology clots Examination of tissues in order to identify or exclude morphological and cytological abnormalities for the purpose of Tissue processing : HISSOP58 Cut up of large specimens by the pathologists Testis HISSOP56 Cut up of large specimens by the pathologists Skin HISSOP59 Cut up of large Breast HISSOP52 Cut up of large Spleen HISSOP47 Cut up of large Bone HISSOP48 Cut up of large Kidney HISSOP53 Cut up of large Appendix Tissue Processing using Sakura VIP and HISSOP69 Processing VIP1&2 HISSOP85 VIP 1&2 maintenance HISSOP66 Processing VIP3 HISSOP88 VIP3 maintenance CPF80 VIP usage maintenance log HISSOP65 Removal of blocks from VIPs HISSOP84 VIP failure Assessment Manager: VB Page 3 of 13
4 Tissue: biopsy, excision, resection, and post-mortem specimens Formalin Fixed Paraffin wax blocks of processed tissue Examination of tissues in order to identify or exclude morphological and cytological abnormalities for the purpose of De-calcification Tissue embedding Microtomy De-calcification using HISSOP70 De-calcification CPF69 Decal end point test Tissue Tek 5EC and HISSOP10 Embedding procedure Tissue sectioning using Tissue Tek and Leica microtomes and HISSOP9 The sectioning procedure CPF18 Histology cutting list Tissue for frozen sections Cryotomy Cryotomy Intra-operative frozen sections using Bright Cryostat and HISSOP1 Frozen section procedure HISSOP50 Cut up of large specimens by the pathologist - Neurosafe FFPE slides (Formalin fixed paraffin embedded) and frozen sections Routine morphological staining For the detection of: Basophilic and eosinophilic structures Routine morphological staining using Sakura Tissue Tek Prisma and HISSOP64 Operation of the Prisma stainer and cover slipper HISSOP1 Frozen section procedure Assessment Manager: VB Page 4 of 13
5 Special stains for the detection of: Documented in-house procedures for manual special stains Frozen Sections Lipids Fat Stains on Frozen Sections (Oil Red O) using HISSOP115 Oil Red O FFPE slides Acid mucopolysaccharides Alcian Blue/PAS using HISSOP14 Alcian Blue PAS Acid and Neutral Mucopolysaccharides Helicobacter Pylori Neutral Mucopolysaccharides, glycogen and fungus Elastic fibres and connective tissue Helicobacter/Microorganisms Gram positive and negative microorganisms Fungi (Aspergillus) and Pneumocystis Elastic fibres and connective tissue Connective tissue Melanin, Argentaffin cells and Lipofuscin pigment Alcian Blue using HISSOP17 Alcian Blue Cresyl Violet Acetate using HISSOP16 Cresyl Violet Acetate Diastase PAS using HISSOP19 Diastase PAS Elastic van Gieson using HISSOP 11 Elastic Van Gieson Giemsa using HISSOP30 Giemsa Gram using HISSOP20 Gram Grocott using HISSOP13 Grocott Haematoxylin Van Gieson using HISSOP12 Haematoxylin Van Gieson Masson Trichrome using HISSOP22 Masson Trichrome Masson Fontana using HISSOP21 Masson Fontana Assessment Manager: VB Page 5 of 13
6 FFPE slides Special stains for the detection of: Documented in-house procedures for manual special stains Melanin Connective tissue and fibrin Copper assoc. Protein and Hepatitis B Neutral Mucopolysaccharides, glycogen and fungus Reticulin fibres Mast cells Melanin Bleach using HISSOP113 Melanin bleach Martius Scarlett Blue using HISSOP25 MSB Orcein using HISSOP26 Orcein PAS using HISSOP18 PAS Reticulin using HISSOP28 Reticulin Toludine Blue using HISSOP114 Toluidine blue FFPE slides Calcium Von Kossa using HISSOP29 Von Kossa Tubercle bacilli Tubercle bacilli Ziehl Neelson using HISSOP23 Ziehl Neelson HISSOP24 Modified Ziehl Neelson Assessment Manager: VB Page 6 of 13
7 FFPE slides Immunohistochemistry to detect the following: Smooth muscle cells, myofibroblasts, myoepithelial cells, Diagnosis of leiomyomas, leiomyosarcomas Broad band cytokeratin marker, epithelial cell carcinomas HGPIN, prostate adenocarcinoma BCL-2 oncoprotein BCL-6 gene Epithelial cell types Ovarian cancer Carcinomas, RCC Reacts with human calretinin and intracellular calcium binding protein. Marker for mesotheliomas T cells Mature T cells, T cell lymphomas Granulocytes Reed-Sternberg cells Mature B cells, Follicular dendritic cells Leica Bond III and Leica Bond Max using HISSOP97 Bond III staining HISSOP98 Bond Max staining HISSOP106 Antibody optimisation and validation Incorporating the following antibodies Actin AE1/3 AMACR/P63 BCL-2 BCL-6 BerEP4 CA125 CA-IX Calretinin CD3 CD5 CD10 CD20 Assessment Manager: VB Page 7 of 13
8 FFPE slides Immunohistochemistry to detect the following: B cells, some T cells B Cells, Reed-Sternberg cells Reed-Sternberg cells and ALCL Haematopoietic cells, vascular endothelium T cells and some soft tissue Leukocyte common antigen Neuroendocrine cells Macrophages B lymphocytes GIST Plasma cells Adenocarcinoma and carcinoid (intestinal epithelium) CEA glycoproteins in adenocarcinoma Neuroendocrine tumours Leica Bond III and Leica Bond Max using HISSOP97 Bond III staining HISSOP98 Bond Max staining HISSOP106 Antibody optimisation and validation Incorporating the following antibodies CD21 CD23 CD30 CD31 CD34 CD45 CD56 CD68 CD79 CD117 CD138 CDX-2 CEA Chromogrannin Assessment Manager: VB Page 8 of 13
9 FFPE slides Immunohistochemistry to detect the following: Stratified squamous epithelium, basal cells, mesotheliomas Normal and neoplastic epithelia Glandular and transitional epithelium Basal cell carcinoma, squamous cell carcinoma Adenocarcinomas Adenocarcinomas, columnar epithelial cells Striated and smooth muscle cells GIST Normal duct epithelial cells, ductal carcinoma Normal and neoplastic epithelium Nuclei of cells containing a high level of oestrogen V.W factor in endothelial cells Mesothelial Cells Thyroid carcinomas Leica Bond III and Leica Bond Max using HISSOP97 Bond III staining HISSOP98 Bond Max staining HISSOP106 Antibody optimisation and validation Incorporating the following antibodies CK5 CK7 CK8/18 CK14 CK19 CK20 Desmin DOG-1 E-Cadherin EMA ER Factor VIII HBME-1 Assessment Manager: VB Page 9 of 13
10 FFPE slides Immunohistochemistry to detect the following: Melanoma, melanocyte differentiation Proliferating Cells B cells and plasma cells B cells and plasma cells Melanoma marker Epithelial tissue from glandular to stratified squamous Peripheral nerves, neuroendocrine tumours Seminomas HPV driven tumour eg Squamous cell carcinoma Neoplastic cells in epithelium Squamous epithelium Nuclei of cells showing expression of Progesterone Prostate secretory and ductal epithelium Leica Bond III and Leica Bond Max using HISSOP97 Bond III staining HISSOP98 Bond Max staining HISSOP106 Antibody optimisation and validation Incorporating the following antibodies HMB45 Ki67 Kappa Lambda Melan A MNF116 NSE Oct3/4 P16 P53 P63 PR PSA Assessment Manager: VB Page 10 of 13
11 FFPE slides Immunohistochemistry to detect the following: Prostate secretory and ductal epithelium Schwann cells, nerve processes, S100 +ve neoplasms eg Melanoma Smooth muscle cells, myoepithelial cells Neuroendocrine cells TTF-1 found in lung and thyroid (thyroid follicular cells) Cells of mesenchymal origin Plasma cells Epithelial cells and smooth muscle in fallopian tube. Wilms tumour Leica Bond III and Leica Bond Max using HISSOP97 Bond III staining HISSOP98 Bond Max staining HISSOP106 Antibody optimisation and validation Incorporating the following antibodies PSAP S100 SMM Synaptohphysin TTF1 Vimentin VS38c WT1 Interpretive/diagnostic microscopy Using HISSOP95 Reporting of Cellular Pathology requests CPM24 Procedure for issuing an amended report HISSOP100 Giving results over the telephone CPM23 Authorisation of cellular Pathology results Assessment Manager: VB Page 11 of 13
12 HUMAN BODY FLUIDS Fine needle aspirates Breast, sputum, urine CSF, oesophageal washings and brushings, Conventional Bronchial Brushings, Serous fluids, Bronchial washings and Bronchial Alveolar lavage samples Cytopathology (Non-Gynae) examination activities for the purposes of clinical Examination of cellular material in order to identify or exclude morphological and cytological abnormalities Demonstration of : Cells (nuclei and cytoplasm) and micro organisms : Diagnostic cytology (Non-gynae) in house documented manual procedures for preparation and screening and reporting using Hologic-T2000, Sakura DRS 2000 CYTSOP5 General procedure for the receipt and processing of nongynaecological specimens CYTSOP15 Preparation of breast samples CYTSOP16 Preparation of Bronchoscopy specimens CYTSOP17 Preparation of cerebrospinal fluid (CSF) CYTSOP18 Preparation of EBUS samples CYTSOP19 Preparation of Head and Neck samples CYTSOP20 Preparation of Lymph node aspirates CYTSOP21 Preparation of natural and induced clots CYTSOP22 Preparation of Serous fluids CYTSOP23 Preparation of Sputum samples CYTSOP24 Preparation of Urine samples Assessment Manager: VB Page 12 of 13
13 HUMAN BODY FLUIDS Fine needle aspirates Breast, sputum, urine CSF, oesophageal washings and brushings, Conventional Bronchial Brushings, Serous fluids, Bronchial washings and Bronchial Alveolar lavage samples Cytopathology (Non-Gynae) examination activities for the purposes of clinical Examination of cellular material in order to identify or exclude morphological and cytological abnormalities Demonstration of: Cells (nuclei and cytoplasm) and micro organisms : Diagnostic cytology (Non-gynae) in house documented manual procedures for preparation and screening and reporting using Hologic-T2000, Sakura DRS 2000 CYTSOP5 General procedure for the receipt and processing of nongynaecological specimens CYTSOP11 Operation of the centrifuge CYTSOP12 Operation of the safety cabinet CYTSOP13 Operation of the Thin Prep 2000 processor CYTSOP30 Screening of nongynaecological preparations CYTSOP14 Operation of the tissue tek DRS staining machine Papanicalou stain for non-gynae specimens and CYTSOP33 Papanicolaou staining method May-Grunwald Giemsa staining for air dried slides using CYTSOP7 May Grunwald Giemsa staining method Diff Quick staining technique using CYTSOP2 Diff - quick staining procedure for FNA s END Assessment Manager: VB Page 13 of 13
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