Charles Halsey, DVM, PhD, DACVP Pfizer, Inc. IHC Resources

Transcription

1 Charles Halsey, DVM, PhD, DACVP Pfizer, Inc. IHC Resources 1

2 IHC Identification Targets Specimens Controls 2

3 Tissue controls Trouble Spots 3

4 The Key to Description IHC Description 4

5 Intermediate Filaments Intermediate Filaments What are they? 5

6 Limitations of Intermediate Filaments Cytokeratin Type II (basic) distribution Type I (acidic) CK1 Keratinized squamous epithelium CK10 CK3 Cornea CK12 CK5 Nonkeratinized epithelium CK13 CK6 Basal cells of glandular and squamous epithelium, CK14, CK17 myoepithelium, mesothelium CK7, CK8 Simple epithelia, superficial squamous epithelia CK16 Simple epithelia of GI track, Merkel cells, urothelium CK20 6

7 Cytokeratin Keratin Expression Keratin Expression 7

8 Respiratory epithelium, horse Complex adenoma, cat Colonic Adenocarcinoma, dog 8

9 Ocular neoplasm, cat Ciliary body adenocarcinoma Vimentin 9

10 Vimentin Expression Vimentin Expression Splenic lymphoma, dog 10

11 Complex mammary gland adenoma, dog Dual Expression of Intermediate Filaments Tibiotarsal joint, dog 11

12 Vimentin Keratin DX: Synovial sarcoma Hepatic capsule, cat 12

13 Vimentin Keratin DX: Mesothelioma Desmin 13

14 Desmin Expression Desmin Gingiva, dog 14

15 Gingiva, dog Neural Fibrillary Protein (NFP) NFP Expression 15

16 Cerebral mass, dog Veterinary Pathology, 41:3, pp NFP Expression Ganglioneuroblastoma Glial Fibrillar Acidic Protein (GFAP) 16

17 Mass, subcutis, cat GFAP Peripheral nerve sheath tumor Nestin 17

18 Lysozyme Other Macrophage Markers Kidney, dog 18

19 Multifocal vimentin + with a positive internal control Lysozyme Histiocytic sarcoma Factor VIII Related Antigen 19

20 Cutaneous neoplasm, dog Factor VIII Antigen Hemangiosarcoma Platelet Endothelial Cell Adhesion Molecule (PECAM) (CD31) 20

21 Thyroglobulin Thyroid Gland Thyroid transcription factor-1 (TTF-1) Calcitonin Neoplasm, neck, dog Thyroglobulin Thyroid follicular carcinoma 21

22 Thyroid mass, baboon Calcitonin Medullary carcinoma S-100 Protein 22

23 Neoplasms that express S-100 Subcutaneous mass, cat S-100 Protein Peripheral nerve sheath tumor 23

24 Neuron Specific Enolase (NSE) Synaptophysin and Chromogranin Synaptophysin Pancreatic islet cell, dog 24

25 Chromogranin Adrenal medulla, dog Pharyngeal neoplasm, dog Synaptophysin Neuroendocrine carcinoma 25

26 Muscle Markers Actin or muscle specific actin Smooth muscle actin Myoglobin Actin Smooth Muscle Actin 26

27 Myoglobin S-100+ Neural crest origin GFAP + PNST MelanA, HMB45, PNL2 +,TRP2 Melanoma Spindle cell neoplasm Actin, desmin + Muscle origin SMA + Smooth muscle Myoglobin + Skeletal or cardiac muscle Factor VIII or CD31 + Vascular origin Neoplasm thoracic wall, rat 27

28 Actin Desmin Myoglobin 28

29 Rhabdomyosarcoma Gastric submucosa, dog 29

30 Diffuse vimentin immunoreactivity Diffuse desmin immunoreactivity Diffuse actin immunoreactivity 30

31 Diffuse smooth muscle actin immunoreactivity Negative staining for CD117 Negative staining for GFAP 31

32 DIAGNOSIS? Myoepithelial Markers Mammary neoplasm, cat 32

33 Calponin Complex adenoma Leukocyte and Platelet Markers CD 1a CD 3 CD 18 CD 45RA CD 56 Leukocyte and Platelet Markers 33

34 CD 3+ T cell lymphoma Round cell neoplasm CD79a, BLA 36, CD45RA, CD20 B cell lymphoma Kappa, lambda light chains Plasma cell tumor Lysozyme, CD68, MAC 387 Histiocytic Duodenal mass, dog Vet. Path., 39:5, pp CD

35 Mast cell tryptase Dx: Mast Cell Tumor Cutaneous neoplasm, dog CD 3 DX: Epitheliotropic lymphoma 35

36 Mass on the lip, dog CD 3 Interpretation? CD 79a DX: B-cell Lymphoma 36

37 Diagnosis of Melanomas Oral neoplasm, dog Melan A DX: Malignant Melanoma 37

38 Infectious Disease Diagnosis MERS-Coronavirus; Lung Lassa, brain 38

39 Ebola Zaire (Guinea strain), liver MERS Coronavirus Morphometric analysis 39

40 Example Haired skin, dog 40

41 Vimentin GFAP S

42 Haired skin: Peripheral Nerve Sheath tumor Self-exam - Tissue from a dog H&E Tissue from a dog Vimentin 42

43 Tissue from a dog Melan-A Tissue from a dog S-100 protein Tissue from a dog Myoglobin 43

44 Tissue from a dog Desmin Self-evaluation #2 - Tissue from a dog rhabdomyosarcoma The End. 44