Schedule of Accreditation issued by United Kingdom Accreditation Service High Street, Feltham, Middlesex, TW13 4UN, UK

Transcription

1 21-47 High Street, Feltham, Middlesex, TW13 4UN, UK Cellular Pathology Pathology Department Northampton General Hospital Cliftonville Northampton Northamptonshire NN1 5BD Contact: Mary Pendleton Tel: +44 (0) Fax: +44 (0) Website: Clinical/Pathology/Pathology.aspx below Additional site activity performed away from the laboratory Location details Activity On-site Mortuary and Body Store Storage of bodies Retrieval of tissue via post mortem Record keeping (body identification and audit trail) Assessment Manager: LD1 1 of 14

2 DETAIL OF ACCREDITATION FLUIDS Tissue samples Examination of tissues in order to identify or exclude morphological and cytological abnormalities for the purpose of diagnosis including identification of basophilic and eosinophilic structures Tissue dissection: CPH001 - Cut-up Manual - Routine Specimens CPH019 - Cut-Up Manual - Non- Routine Specimens CPH172 - Dissection Manual For B Specimen CPH173 A Specimen Dissection Decalcification: CPH024 Rapid Decalcification Tissue processing - Sakura Tissue Tek VIP 6: CPH038 Processors - Sakura Tissue Tek VIP 6 CPH038EL Tissue Tek VIP 6 CPH067 Block Reprocessing Embedding - Sakura embedding centres: CPH011 Embedding Frozen sections - Leica cryostat: CPH032 Frozen sections Microtomy - Leica Microtomes: CPH013 Microtomy Automated: Leica staining machine: CPH198 Slide Staining, Coverslipping and Collation CPH108 Haematoxylin and Eosin Paraffin Sections Assessment Manager: LD1 Page 2 of 14

3 FLUIDS Tissue samples Special staining for identification of: Manual rapid (frozen sections): CPH032 Frozen sections Manual techniques Acid mucins Acid mucins and neutral mucins Amyloid Basement membranes Basement membranes, fungi & other carbohydrates Blood vessels, elastin Calcium Salts Connective tissue Copper, hepatitis infection Fat Alcian blue staining technique for acid mucins CPH090 Alcian Blue Periodic Acid Schiff (AB PAS) for Acid mucins and neutral mucins CPH091 Congo Red Staining Technique for Amyloid CPH095 Grimelius Technique CPH105 Periodic acid Schiff Staining Technique with diastase digestion CPH128 Verhoeff s Elastic Van Gieson (EVG) Staining Technique CPH144 Von Kossa Demonstration Technique for Calcium Salts CPH147 Martius Scarlet Blue (MSB) CPH116 Masson Trichrome CPH118 Van Gieson Stain CPH143 Orcein Stain CPH124 Oil Red O CPH123 Assessment Manager: LD1 Page 3 of 14

4 FLUIDS Tissue samples Special staining for identification of: Fungi Elastin and connective tissue Helicobacter Pylori Iron storage disease Leprosy bacilli Mast Cells Melanin Melanin Reticulin Spirochetes, Helicobacter pylori Tubercle Bacilli Manual techniques Grocott's Hexamine Silver CPH107 Miller s Elastic Van Gieson (EVG) Giemsa staining technique for Helicobacter Pylori CPH102 Colloidal Iron (after Hale) CPH155 Wade Fite staining technique for leprosy bacilli CPH152 Toluidine Blue Staining Technique for Mast Cells CPH141 Bleaching technique for Melanin CPH093 Masson Fontana Fontana reaction for melanin CPH117 Gordon and Sweets silver impregnation technique for reticulin CPH103 Warthin Starry Stain CPH149 Ziehl Neelsen (ZN) Staining Technique for Tubercle Bacilli CPH151 Assessment Manager: LD1 Page 4 of 14

5 FLUIDS Tissue samples Special staining for identification of: identification of: Anaplastic lymphoma kinase Embryonic cell in normal tissues and germ tumours Epithelial cells Epithelial cell types B cells, plasma cells folicular lymphomas and lymphomas Ovarian epithelial malignancies, seminal vesical carcimoma, anaplastic lymphoma Parafollicular cells in thyroid and medullary thyroid carcinomas Smooth muscle cells Marker for mesotheliomas Mature B cells, follicular dentritic cells, CALLA Manual techniques CPH160 Operation of Bond 3 Immunostainer ALK (CD246) Alpha Fetoprotein AUA-1 BerEP4 BOB-1 Bcl-2 Bcl-6 CA125 Calcitonin Calponin Caldesmon Calretinin CD10 Assessment Manager: LD1 Page 5 of 14

6 FLUIDS identification of: Various - mast cells, stem cells, Cajal cells, melanocytes, basal cells (skin) and mammary duct epithelium CD117 Plasma cells CD 138 Granulocytes, Reed-Sternberg cells Monocytes Langerhans cells B cell Mature B cells, Follicular dentritic cell B cells, some T cells T cells Reed-Sternberg cells, plasma cells Endothelial cells Leucocyte differentiation T cells (helper & mature) T and B cell Lymphoma's Leucocytes Mature T cells, T cell lymphomas CD15 CD163 CD1a CD20 CD21 CD23 CD3 CD30 CD31 CD34 CD4 CD43 CD45 CD5 Assessment Manager: LD1 Page 6 of 14

7 FLUIDS identification of: Natural killer cells, neuroendocrine tissues Platelets, megakaryocytes, macrophages, endothelila cells T cells. Natural killer cells, thymocytes T cells CD56 CD57 CD68 CD79a Wide range of tumours including Ewings Saracoma, & T cell lymphoma Intestinal epithelial cells, colorectal + other adenocarcinomas CEA glycoproteins CD8 CDX2 CEA CD99 Tissues of neuroendocrine origin Squamous and epithelial phynotypical tumours Adenocarcinomas, columnar epithelial cells Basal cells, filamentous keratins form glandular to stratified squamous, prostate adenocarcinoma Mesothelioma Normal and neoplastic epithelia Chromogranin A CK14 CK20 CK34βE1/2 (HMW) CK5 CK7 Assessment Manager: LD1 Page 7 of 14

8 FLUIDS identification of: Epithilial cells CMV Investigation of tumorigenesis, especially useful for identifying Mantle cell Lymphoma CK8/18 (cam5.2) CMV Cyclin D1 Lymphatic vessels D2-40 B cell specify neoplasm Striated and smooth muscle cells Gastro Intestinal Stromal tumours (GIST) Normal duct epithelial cells, ductal carcinoma Epithelial marker, documented increased expression in a large number of neoplasms Normal and neoplastic epithelium Eostrogen receptors, breast carcinoma Breast carcinoma Schwann cells, enteric glial cells, astrocytomas, ependymomas Placental trophpblasts DBA 44 (LHC) Desmin DOG-1 E-cadherin EGFR EMA ER GCDFP GFAP HCG Assessment Manager: LD1 Page 8 of 14

9 FLUIDS identification of: Hepatocellular carcinoma Melanoma, melanocyte differentiation Specific for alpha chains of human IgA Specific for gamma chains of human IgG Specific for mu chains of human IgM Sex chord stromal tumours B cells and plasma cells Proliferating Cells B cell follicles Epstein-Barr virus Mast cells Epidermal differentiation Melanoma marker Pan CytoKeratin marker Soft tissue tumours with muscle differentiation Hepatocyte HMB45 IgA IgG IgM Inhibin Kappa Ki-67 Lambda LMP Mast Cell Tryptase MCK (Ae1/3) Melan A MNF116 MSA Assessment Manager: LD1 Page 9 of 14

10 FLUIDS identification of: Lympho-haemopoietic tumours Histiocytic mediated myeloid leukaemia Discrimination between lymphoid and myeloid leukemias and classification of neoplastic tissue Rhabdomyoblastic differentiation Peripheral nerves, neuroendocrine tumours Neuroendocrine cells B cell germinal centre HPV Prostatic carcinoma Cell proliferation Hydatidiform moles Prostate basal cells, proliferative cells, glandular epithelium Neurones and neuro-endocrine cells Differentiation of tumours with placental origin MUM-1 Muramidase Myeloperoxidase Myoglobin Myosin NSE OCT-2 P16 CIN P504/p63 P53 P57 P63 PGP9.5 Placental Lactogen Assessment Manager: LD1 Page 10 of 14

11 FLUIDS identification of: Progesterone receptors, breast carcinoma Prostate secretory and ductal epithelium Glandular Prostatic epithelium Renal neoplasm High grade PIN, Schwann cells, nerve processes, S100 +ve neoplasms Identifying primary and metastatic carcinoid tumours Smooth muscle cells Neuroendocrine cells Leukaemias, acute lymphocytic lymphomas Mesothelioma Thyroid follicular epithelial cells TTF-1 found in lung and thyroid (thyroid follicular cells) Melanocytic tumours PR PSA PSAP RCC S100 Serotonin SMA Synaptophysin TDT Thrombomodulin Thyroglobulin TTF-1 Tyrosinase Assessment Manager: LD1 Page 11 of 14

12 FLUIDS FLUIDS Body fluids / Fine needle aspirations, Urines and Bronchial Aspects identification of: Cells of mesenchymal origin Epithelial cells and smooth muscle in fallopian tube Endothelial cells and tumors of vascular origin Wilms tumour Macrophages, neutrophils, A1AT accumulation Cytopathological examination of tissues and fluids for the purpose of diagnosis Examination of tissues and fluids in order to identify or exclude morphological and cytological abnormalities for the purpose of diagnosis Vimentin Von Willebrand Factor Wilms Tumour α-1-antitrypsin Interpretive/diagnostic microscopy - Olympus/Leica/Nikon microscopes. According to RCPath Guidelines GCP419 Consultant Cut Up and Reporting Sample preparation: CPC204 Preparation of Brochial and Sputum Specimens CPC205 Preparation of Serous Fluids CPC206 Cell Block Preparation Assessment Manager: LD1 Page 12 of 14

13 FLUIDS Cytopathological examination of tissues and fluidsfor the purpose of diagnosis Examination of tissues and fluids in order to identify or exclude morphological and cytological abnormalities for the purpose of diagnosis CPC207 Preparation of Cerebrospinal Fluid CPC209 Urine Preparation CPC210 Joint Fluid Preparation Staining for nuclear detail in cells present CPC211 Preparation of slides for Immunohistochemistry CPC212 Preparation of FNAs and Needle Washings CPC213 Preparation of Serous Fluids by the ThinPrep Method CPC214 Histopaque Preparation Method CPC108 Glacial Acetic Acid method for bloodstained samples Staining: Automated - Leica Autostainer XL CPC107 & CPC301 Papanicolaou Staining method Leica Autostainer XL Manual CPC305 Hema-Gurr Rapid staining technique CPC 300 May Grunwald Giemsa Assessment Manager: LD1 Page 13 of 14

14 FLUIDS Body fluids / Fine needle aspirations, Urines and Bronchial Aspects cont d Cytopathological examination of Examination of tissues and fluids in order to identify or exclude morphological and cytological abnormalities for the purpose of diagnosis Interpretive/diagnostic microscopy: Nikon Microscopes CPC221 - Screening and Reporting Non-Gynae Cytology Specimens GCP419 Consultant Cut Up and Reporting END Assessment Manager: LD1 Page 14 of 14