Versatile hybrid silica based (U)HPLC columns. YMC-Triart

Transcription

1 INCREASED TEMPERATURE STABILITY! The Selectivity Company Versatile hybrid silica based (U)HPLC columns YMC-Triart (U)HPLC microlc LC/MS

2 YMC-Triart C hromatographers always seek to push the limits of HPLC columns to greater extremes to allow them to perform day-to-day with ever-changing ph, buffers and temperature ranges. The column for the laboratory of today must be suitable for harsh ph conditions in combination with high temperature ranges without sacrificing selectivity. In order to meet these goals, YMC has developed a novel particle technology. The revolutionary production technique provides a silica-organic hybrid stationary phase, which provides an outstandingly narrow pore size and particle size distribution. This in turn, results in low back pressures and high loadability. Industry Solution Pharmaceutical QA / QC Drug Discovery Isolation to Purification Manufacturing Life Science Amino Acids Peptides to Proteins / Antibodies Oligonucleotides Food & Beverages Food Safety QA / QC Environmental 2

3 Contents page Phases Specifications Transfer between HPLC UHPLC Applications UHPLC... 7 Pharmaceuticals LC / MS Food Life Sciences Polar Analytes / AQ Phase HILIC QC Data Column hardware for bioseparations Substance index Ordering information

4 Triart Phase overview YMC-Triart C8 YMC-Triart C8 ExRS YMC-Triart C8 YMC-Triart Bio C4 Si Si Si Si versatile applications extended ph and stability alternative to C8 proteins/antibodies/peptides first choice for method development hydrophobic substances 3 Å widepore short retention time positional isomers ph -2/9 C max. % aqueous eluents ph -2/9 C max. YMC-Triart Phenyl ph -/9 C max. ph -2/9 C max. YMC-Triart PFP % aqueous eluents YMC-Triart Diol-HILIC F Si % aqueous eluents HO OH aromatic compounds (π-electron donor) cis-trans isomers conjugated systems OH OH HO aromatic compounds (π-electron donor) OH polar halogenated compounds % aqueous eluents Si OH F OH F F HO HO HO Si F OH HO OH very polar compounds less ionic adsorption % aqueous eluents In order to offer a convenient solution for method developers YMC is offering price attractive Method Development Kits with a selection of 3 different YMC-Triart (U)HPLC columns. 4

5 Specification Triart Specification YMC-Triart C8 YMC-Triart C8 ExRS YMC-Triart C8 YMC-Triart Bio C4 YMC-Triart Phenyl YMC-Triart PFP YMC-Triart Diol-HILIC Base organic/inorganic silica Stationary phase C8 (USP L) C8 (USP L) C8 (USP L7) C4 (USP L26) Phenyl (USP L) Pentafluorophenyl (USP L43) Diol (USP L2) Particle size.9, 3 and 5 µm Pore size 2 nm 8 nm 2 nm 3 nm 2 nm 2 nm 2 nm Specific surface 36 m 2 /g 43 m 2 /g 36 m 2 /g 36 m 2 /g 36 m 2 /g 36 m 2 /g Carbon content 2% 25% 7% 7% 5% Bonding trifunctional Endcapping multi-stage multi-stage multi-stage multi-stage multi-stage none none ph range ~ 2 ~ 2 ~ 2 ~ ~ ~ 8 2 ~ Temperature range ph < 7: 9 C ph > 7: 5 C ph < 7: 9 C ph > 7: 5 C ph < 7: 9 C ph > 7: 5 C ph < 7: 9 C ph > 7: 5 C 5 C 5 C 5 C Pressure limit * previous hardware, still available [2/25 MPa (3,/3,75 psi)].9 μm: MPa (5, psi) 3/5 μm: 45 MPa (6,525 psi)* % aqueous eluents Particle technology YMC-Triart is a versatile material prepared using tightly controlled particle formation technology which has been adapted from micro-reactor technology. This recently developed production process developed by YMC results in exceptionally narrow particle and pore size distributions. With YMC-Triart, challenging ph and high temperature conditions are no longer a limitation to the day-to-day work in laboratories. Most importantly, due to its unique particle composition, a balanced hydrophobicity and silanol activity are achieved which makes YMC-Triart a First Choice column in method development. 5

6 "First choice" column for method development Hydrophilic ODS Hydrogen bonding capacity a (caffeine/benzene) Hydrosphere C8 Pro C8 Triart C8 Pro C8 Triart C8 Symmetry C8 Standard ODS Hydrophobic ODS ZORBAX Extend-C8 Pro C8 RS Triart C8 ExRS Hydrophobicity k (amylbenzene) 6. C onventional hybrid silica-based ODS columns tend to be less hydrophobic than silica-based columns. YMC-Triart C8 has a higher carbon load, giving it a hydrophobicity comparable to that of standard ODS columns, thereby making it a versatile first-choice column for method development. On the other hand, YMC-Triart C8 ExRS has been designed to provide contrastingly different separation characteristics! Hydrogen bonding capacity a (caffeine/benzene) Triart Bio C4 Hydrophobicity 6

7 Versatile wide ph stability Phosphate buffer (ph.5, 4 C)* % TFA (ph, 7 C)* ph & temperature YMC-Triart C8 ExRS YMC-Triart C8/C8 YMC-Triart Bio C4 Hybrid based C8 Hybrid based C8 Silica based C8 Column: YMC-Triart (5 µm) 5 x 4.6 mm ID Part No.: TA2S5-546WT Eluent: 5 mm K 2 HPO 4 -K 3 PO 4 (ph.5) / methanol (9/) Flow rate:. ml/min Temperature: 4 C Sample: benzyl alcohol Column: YMC-Triart C8 (5 µm) 5 x 2. mm ID Part No.: TA2S5-52WT Eluent: acetonitrile / water (6/4) Flow rate:.2 ml/min Temperature: 37 C Sample: butyl benzoate Stability at high temperature ph 6.9, 7 C* ph, 9 C* 2% YMC-Triart Bio C4 Rate on N [%] % 8% 6% 4% Silica based C8 YMC-Triart C8 Silica based C8 alkaline-resistant type % of inital retention Monofunctional C4 2% 6 % Time [h] Time of exposure(hr) Column: YMC-Triart C8 (5 µm) 5 x 2. mm ID Part No.: TA2S5-52WT Eluent: 2 mm KH 2 PO 4 -K 2 HPO 4 (ph 6.9) / acetonitrile (9/) Flow rate:.2 ml/min Temperature: 7 C Sample: phenol Column: YMC-Triart Bio C4 Column (5 µm) 5 x 3. mm ID Part No.: TB3S5-53PTH Eluent: acetonitrile/water (6/4) Flow rate:.4 ml/min Temperature: 37 C Sample: butyl benzoate YMC-Triart phases show great chemical stability due to the highly developed hybrid-silica matrix. Even under high ph or high temperature conditions, the lifetime of YMC-Triart phases is more than x greater than conventional reversed phase columns. 7

8 Transfer HPLC UHPLC Secure your method transfer!* Differences in selectivity, retention time, and also peak shapes between different particle sizes of commercially available C8 phases in the same brand (or an alternative as recommended by its manufacturer) have been observed. YMC-Triart C8 3 µm TF(2).43.9 µm TF(2).47 YMC has addressed this issue of method transfer. YMC-Triart columns show identical selectivity and excellent peak shapes for basic compounds for all 3. µm to.9 µm particle sizes. It allows predictable scale up from UHPLC to conventional HPLC and even to semi-preparative LC, and vice versa. Case Studies** X-Bridge BEH C8 and Acquity UPLC BEH C8 2.5 µm TF(2) µm TF(2) 2.35 ** These observations might not be representative for all applications. Kinetex TM C8 2.6 µm TF(2) µm TF(2) 4.94 Kinetex TM C8 columns show significant peak tailing and have limited scalability due to lack of larger particle sizes. Column: 5 x 2. mm ID or 2. mm ID Eluent: 2 mm KH 2 PO 4 -K 2 HPO 4 (ph 6.9) / acetonitrile (65/35) Temperature: 4 C Flow rate:.2 ml/min Detection: UV at 235 nm. Chlorpheniramine (basic) 2. Dextromethorphan (basic) 3. Propyl paraben (internal standard) 8

9 Transfer HPLC UHPLC Evaluation of method transfer performance!*.2 Kinetex C8 2.6 µm Hydrogen bonding capacity [a (Caffeine/Benzene)]..8 Hypersil GOLD Hypersil GOLD.9 µm Eluent: methanol/water (8/2) [Amylbenzene] methanol/water (3/7) [Caffeine, Benzene] Kinetex C8.7 µm ACQUITY UPLC BEH C8 XBridge C8 L-column2 ODS 2. µm YMC-Triart C8 3 µm.9 µm L-column2 ODS Hydrophobicity [k (Amylbenzene)] With the introduction of UHPLC, sub-2-µm particles became neccessary. Therefore smaller particles have been added to existing column lines. Consequently, sub-2-µm particles may exhibit differences in chromatographic performance. By introducing YMC-Triart, YMC provides matching chromatographic behaviour for all particles sizes! Method transfer HPLC UHPLC* YMC-Triart C8 (5 µm) 5 x 2. mm ID TA2S5-52WT 4.5 MPa (65 psi) 5% B (-3 min) 5-3% B (3- min) 3-45% B (-55 min).2 ml/min. Oxine-copper (Copper 8-quinolinolate) 2. Asulam 3. Thiram 4. Triclopyr 5. Mecoprop 6. Flazasulfuron 7. Siduron 8. Halosulfuron-methyl 9. Azoxystrobin 55 min YMC-Triart C8 (.9 µm) 5 x 2. mm ID TA2SP9-52PT 37 MPa (54 psi) 5% B (-.3 min) 5-3% B (.3-. min) 3-45% B (.-6. min).6 ml/min 6 min Eluent: A) water+.% formic acid B) acetonitrile+.% formic acid Temperature: 4 C Detection: UV at 24 nm Injection: µl (5 µg/ml) When transferring the 55 min HPLC method to UHPLC scale, the resolution remains the same although the separation time is reduced to only 6 min. 9

10 UHPLC Higher resolution and good loadability* YMC-Triart C8.9 µm 2 3 TF(2)=.47 Ascentis Express C8 2.7 µm 2 3 TF(2)=5.5 Kinetex C8 2.6 µm 2,3 TF(2)= µm 2,3 TF(2)=4.94 Column: 5 x 2. or 2. mm ID Eluent: 2 mm KH 2 PO 4 -K 2 HPO 4 (ph 6.9)/ acetonitrile (65/35) Flow rate:.2 ml/min Detection: UV at 235 nm Temperature: 4 C Lower HETP means higher resolution!* HETP (mm/plates),5,,5.9 µm YMC-Triart C8 2.7 µm Ascentis Express (Core-shell type) 2.6 µm Kinetex C8 (Core-shell type).7 µm Kinetex C8 (Core-shell type), Linear velocity (mm/sec) Column: 5 x 2. or 2. mm ID Eluent: acetonitrile / water (6/4) Detection: UV at 254 nm Sample: Butyl benzoate YMC-Triart C8 always shows the lowest HETP compared to the three Core-Shell products over the range of linear velocity applied.

11 UHPLC & MS Determination of artificial sweeteners with LC-MS/MS* Acesulfame (K salt) Saccharin Cyclamate Na Non biological markers of wastewater entries in ground and surface water Extracted Ion Chromatogram (XIC) of Acesulfame K,. µg/l Extracted Ion Chromatogram (XIC) of Saccharin,. µg/l Column: YMC-Triart C8, (.9 µm, 2 nm) x 3. mm ID Part-No.: TA2SP9-3PT LC-System: Agilent HPLC system and CTC Analytics HTC-Pal Autosampler MS/MS System: Applied Biosystems MDS Sciex API 4, ESI negative Temperature: 35 C Flow:.3 ml/min Injection: 4 µl, direct injection Eluent: A: water (containing mmol NH 4 formate) B: methanol (containing mmol NH 4 formate) Gradient: Time % B Extracted Ion Chromatogram (XIC) of Cyclamate Na,. µg/l by courtesy of: Thomas Class, Sandro Jooß, PTRL Europe, Helmholtzstraße 22, Science Park I, D-898 Ulm

12 UHPLC Angiotensin I, II and III Insulin 2. Angiotensin I 2. Angiotensin II 3. Angiotensin III. Bovine insulin 2. Human insulin 3. Porcine insulin Column: YMC-Triart C8 (.9 µm, 2 nm) 5 x 2. mm ID Part No.: TA2SP9-52PT Eluent: 2 mm KH 2 PO 4 + K 2 HPO 4 (ph 7.9) / acetonitrile (22/78) Flow rate:.7 ml/min Detection: UV at 22 nm Pressure: 72 bar Injection:.5 µl Temperature: 4 C Column: Part No.: Eluent: Gradient: Flow rate: Detection: Pressure: Injection:.5 µl Temperature: 3 C YMC-Triart C8 (.9 µm, 2 nm) 5 x 2. mm ID TA2SP9-52PT A) H 2 O +.% TFA B) acetonitrile +.% TFA 3% B ( min); 3-32% B (-5 min); 32% B (55 min).6 ml/min UV at 22 nm 6 bar Macrolide antibiotics Sulpha drugs. Erythromycin 2. Spiramycin 2. Uracil 2. Sulphathiazole 3. Sulphamerazine 4. Sulphamethoxazole Column: YMC-Triart C8 (.9 µm, 2 nm) 5 x 2. mm ID Part No.: TA2SP9-52PT Eluent: A) 2 mm K 2 HPO mm KH 2 PO 4 (ph 7.9) B) acetonitrile Gradient: 6% B (.5 min); 6-7% B (.5-.5 min); 7% B (3.5 min) Flow rate:.45 ml/min Detection: UV at 2 nm Pressure: 52 bar Injection: µl Temperature: 5 C Column: YMC-Triart C8 (.9 µm, 2 nm) 5 x 2. mm ID Part No.: TA2SP9-52PT Eluent: H 2 O + formic acid (ph 2.5) / acetonitrile (75/25) Flow rate:.75 ml/min Detection: UV at 28 nm Pressure: 74 bar Injection:.5 µl Temperature: 5 C 2

13 UHPLC Tetracycline antibiotics 2. Oxytetracycline 2. Tetracycline 3. Chlortetracycline Betablockers 4. Atenolol 2. Pindolol 3. Nadolol 4. Metoprolol 5. Propranolol Column: YMC-Triart C8 (.9 µm, 2 nm) 5 x 2. mm ID Part No.: TA2SP9-52PT Eluent: 5 mm CH 3 COONH 4 / acetonitrile (87/3) Flow rate:.65 ml/min Detection: UV at 28 nm Pressure: 662 bar Injection: µl Temperature: 4 C Column: YMC-Triart C8 (.9 µm, 2 nm) 5 x 2. mm ID Part No.: TA2SP9-52PT Eluent: A) 2 mm CH 3 COONH 4 + ammonia (ph 9.) B) acetonitrile Gradient: 25% B (. min); 75% B (-6 min) Flow rate:.35 ml/min Detection: UV at 254 nm Pressure: 45 bar Injection: µl Temperature: 4 C Fast LC for conventional HPLC* YMC-Triart C8 (5 µm) 5 x 2. mm ID % B,.2 ml/min, 5.7 MPa 5% B,.2 ml/min, 5.6 MPa Insufficient separation optimisation Baseline separation, but long analysis time Down scaling YMC-Triart C8 (3 µm) 5 x 2. mm ID 5% B,.4 ml/min, 2.2 MPa Fast baseline separation Eluent: A) water / HFBA* (/.) B) acetonitrile / HFBA* (/.) Temperature: 37 C Detection: UV at 29 nm Injection: µl (. mg/ml) *heptafluorobutyric acid 3

14 UHPLC Stevia leaves Nasal spray. Stevioside hydrate 2. Rebaudioside A. Maleic acid sodium salt 2. Tetrahydrozoline HCl 3. Chlorpheniramine maleate 4. Benzethonium chloride Column: YMC-Triart Diol-HILIC (.9 µm, 2 nm) x 3. mm ID Part No.: TDH2SP9-3PT Eluent: acetonitrile / water (85/5) Flow rate: ml/min Detection: UV at 2 nm Injection: 2 µl Temperature: 3 C Column: YMC-Triart C8 (.9 µm, 2 nm) 5 x 2. mm ID Part No.: TA2SP9-52PT Eluent: A) water +.5% TFA / B) methanol (5/5) Gradient: min A B Flow rate:.6 ml/min Detection: UV at 26 nm Injection:.2 µl Temperature: 4 C Eye drop formulation. Maleic acid sodium salt 2. Pyridoxine 3. Neostigmine methylsulfate 4. Chlorpheniramine maleate 5. Naphazoline HCl 6. Glycyrrhizin acid ammonium salt Column: Part No.: Eluent: Flow rate: Detection: Injection:.5 µl Temperature: 4 C YMC-Triart C8 (.9 µm, 2 nm) 5 x 2. mm ID TA2SP9-52PT A) water +.5% TFA B) acetonitrile.6 ml/min UV at 265 nm Gradient: min A B

15 UHPLC Guaiacol and impurities. Pyrocatechol 2. Phenol 3. Guaiacol 4.,2-Dimethoxyl benzene 5. Methyl benzoate Column: YMC-Triart C8 (.9 µm, 2 nm) 5 x 2. mm ID Part No.: TA2SP9-52PT Eluent: water / acetonitrile (5/5) Flow rate:.7 ml/min Detection: UV at 254 nm Injection:.5 µl Temperature: 4 C Paracetamol. 4-Aminophenol 2. Acetaminophenone 3. 2-Acetamidophenol 4. 4-Acetamidoacetophenone 5. Acetanilide 6. 4-Nitrophenol 7. 4-Chloroacetanilide 8. 2-Hydroxyacetophenone Column: YMC-Triart C8 (.9 µm, 2 nm) 5 x 2. mm ID Part No.: TA2SP9-52PT Eluent: A) water + formic acid (ph 2.5) / B) acetonitrile Flow rate:.7 ml/min Detection: UV at 254 nm Injection:.5 µl Temperature: 4 C Gradient: min A B

16 UHPLC 7 Analgesics. Acetaminophene 2. 2-Acetamidophenol 3. Caffeine 4. Acetanilide 5. Acetylsalicylic Acid 6. Phenacetine 7. Salicylic Acid Column: YMC-Triart C8 (.9 µm, 2 nm) 5 x 2. mm ID Part No.: TA2SP9-52PT Eluent: water + formic acid (ph 2.5) / acetonitrile (5/5) Flow rate:.8 ml/min Detection: UV at 24 nm Injection: µl Temperature: 4 C Hydroxychloroquine and chloroquine Column: YMC-Triart C8 (.9 µm, 2 nm) 5 x 2. mm ID Part No.: TA2SP9-52PT Eluent: 2 mm HCOOH-HCOONH 4 (ph 4.3) / acetonitrile (9/) Flow rate:. ml/min Detection: UV at 254 nm Injection: 2 µl ( µg/ml) Temperature: 25 C 6

17 UHPLC Duloxetine and its degradation products* Column: YMC-Triart C8 (5 µm, 2 nm) 5 x 3. mm ID Part No.: TA2S5-53WT Flow rate:.425 ml/min Gradient: -9% B (-36 min) Injection: 6 µl 35 min Column: YMC-Triart C8 (.9 µm, 2 nm) 5 x 2. mm ID Part No.: TA2SP9-52PT Flow rate:.8 ml/min Gradient: -9% B (-3 min) Injection: µl x faster 3 min Eluent: A) mm CH 3 COONH 4 (ph 6.) B) acetonitrile Detection: UV at 23 nm Temperature: 3 C Sample: Oxidative degradation products of duloxetine hydrochloride* * Sample preparation was performed as described by Veera Reddy. Arava et al. Der Pharma Chemica, 22 4 (4):

18 Pharmaceuticals YMC-Triart C8 ExRS High hydrophobicity & high steric recognition ability* Basic Compound Coordination Compound Neutral Compounds Polar p-p interaction. Amitriptyline 2. 8-Quinolinol 3. Testosterone 4. Naphthalene Acidic Compound Hydrophobic 5. Ibuprofen 6. n-propylbenzene 7. n-butylbenzene Steric Cognitive Ability 8. o-terphenyl (steric) 9. Triphenylene (planar) YMC-Triart C8 2 3 YMC-Triart C8 ExRS Column: 5 µm, 5 x 3. ID Part No.: TA2S5-53PTH / TAR8S5-53PTH Eluent: 2 mm HCOOH-HCOONH 4 (ph 4.3) / acetonitrile (9/) Flow rate:. ml/min Detection: UV at 254 nm Injection: 2 µl ( µg/ml) Temperature: 25 C 8

19 Pharmaceuticals YMC-Triart C8 ExRS Omega fatty acid isomers. Eicosapentaenoic acid (C2:5;.5 mm) 2. Alpha-Linolenic acid (C8:3 α;.5 mm) 3. Gamma-Linolenic acid (C8:3 γ;.5 mm) 4. Docosahexaenoic acid (C22:6;.5 mm) 5. Palmitoleic acid (C6:; 3 mm) 6. Arachidonic acid (C2:4;.5 mm) 7. Linoleic acid (C8:2;.5 mm) 8. Oleic acid (C8:; 3 mm) Column: YMC-Triart C8 ExRS (3 μm, 8 nm) mm ID Part No.: TAR8S3-546PTH Eluent: A) A: H 2 O +.5 % H 3 CCOOH B) ACN +.5 % H 3 CCOOH Gradient: % B (-2 min), 78.5% B (2-27 min), % B ( min), 9% B ( min) Flow rate: ml/min Temperature: 35 C Detection: UV at 254 nm Injection: 2 μl Outstanding steric selectivity for chlorophenols mau Chlorophenol 2. 2,6-Dichlorophenol 3. 2,3-Dichlorophenol 4. 2,5-Dichlorophenol 5. 2,4-Dichlorophenol 6. 3,4-Dichlorophenol 7. 3,5-Dichlorophenol min Column: YMC-Triart C8 ExRS (.9 μm, 8 nm) 75 x 3. mm ID Part No.: TAR8SP9-L53PT Eluent: A) water +.% HCOOH B) methanol +.% HCOOH Gradient: 44-5% B (-8. min), 5-5.5% B (8.- min), % B (-. min), 65% B (.-2 min) Flow rate:.7 ml/min Detection: UV at 28 nm Injection: μl (.7 mg/ml) Temperature: 4 C 9

20 Pharmaceuticals YMC-Triart C8 ExRS Structural analogs* mau YMC-Triart C RS = min Higher Resolution mau 4 YMC-Triart C8 ExRS RS = min Column: 5 µm, 5 x 3. mm ID Part No.: TA2S5-53PTH / TAR8S3-53PTH Eluent: THF / acetonitrile (/9) Flow rate:.425 ml/min Detection: UV at 265 nm Injection: 4.25 µl ( µg/ml) Temperature: 3 C 2

21 Pharmaceuticals YMC-Triart C8 ExRS Easy transfer HPLC UHPLC mau 4 HPLC method 5 μm; 5 x 3 mm ID.425 ml/min min % mau UHPLC method.9 μm; 75 x 2. mm ID.2 ml/min 6 min Part No.: TAR8S3-53PTH/TAR8SP9-L5QPT Eluent: THF / acetonitrile (/9) Detection: UV at 265 nm Temperature: 3 C 2

22 Pharmaceuticals YMC-Triart Phenyl Excellent alternative to C8 phases for steroids. Estriol 2. Cortisol / Hydrocortison 3. Prednisone 4. Corticosterone 5. Beta-Estradiol 6. Cortisone / Cortison acetate 7. Alpha-Estradiol 8. 2-Hydroxyprogesterone 9. Estrone. Progesterone Column: YMC-Triart Phenyl (.9 μm, 2 nm) x 2. mm ID Part No.: TPH2SP9-2PT Eluent: A) water B) acetonitrile Gradient: 29-35% B (-9 min), 35-6% B (9-9. min), 6% B ( min) Flow rate:.3 ml/min Detection: UV at 22 nm Injection:.5 μl Temperature: 48 C 22

23 Pharmaceuticals YMC-Triart Phenyl Antidepressants. Nortriptyline 2. Toluol 3. Imipramine 4. Amitriptyline Column: YMC-Triart Phenyl (.9 µm, 2 mm) x 2. mm ID Part No.: TPH2SP9-2PT Eluent: methanol / 25 mm KH 2 PO 4 (ph 6.) (65/35) Flow rate:.4 ml/min Detection: UV at 254 nm Injection: 2 µl Temperature: 25 C UHPLC separation of different benzodiazepines mau a-hydroxyalprazolam 2. Oxazepam 3. Clonazepam Temazepam 5. Diazepam min Column: YMC-Triart Phenyl (.9 μm, 2 nm) x 2. mm ID Part No.: TPH2SP9-2PT Eluent: A) water B) acetonitrile Gradient: 52-54% B (-. min), 54-95% B (.-.2 min), 95% B (.2-3 min) Flow rate:.5 ml/min Detection: UV at 254 nm Injection: 2 μl (.2 mg/ml) Temperature: 35 C 23

24 Pharmaceuticals YMC-Triart PFP Resorcinol 2. Resorcinol 2. Pyrocatechol 3. 2-Methylresorcinol 4. 4-Methylcatechol 5. 2,5-Methylresorcinol 6. 4-Nitrocatechol Column: YMC-Triart PFP (.9 µm, 2 mm) x 2. mm ID Part No.: TPF2SP9-2PT Eluent: water +.% formic acid / acetonitrile +.% formic acid (85/5) Flow rate:.8 ml/min Detection: UV at 27 nm Injection:.5 µl Temperature: 25 C Piperine and its isomers* mau (A) UV at 28 nm O H H O O H H. Chavicine Z,Z-(cis-cis)-piperine N 2 3 O O H H H O H N 2. Isopiperine Z,E-(cis-trans)-piperine mau min (B) UV at 34 nm O O 3. Isochavicine E,Z-(trans-cis)-piperine H H O H H N min H O N H H O 4. Piperine E,E-(trans-trans)-piperine H O Column: YMC-Triart PFP (5 μm, 2 nm) 5 x 3. mm ID Part No.: TPF2S5-53PTH Eluent:.% HCOOH / acetonitrile (6/4) Flow rate:.425 ml/min Injection: 4.25 μl Temperature: 25 C 24

25 Pharmaceuticals YMC-Triart PFP Catecholamines, serotonin, and their precursors and metabolites* Column: YMC-Triart PFP (3 µm, 2 nm) 5 x 3. mm ID Part-No.: TPF2S3-53WT Eluent: A) mm formic acid B) methanol containing mm formic acid Gradient: -2% B (-3 min), 2% B (3-35 min) Flow rate:.425 ml/min Detection: UV at 28 nm Injection: 4 µl (5 µg/ml) Temperature: 25 C 25

26 Pharmaceuticals YMC-Triart C8 Basic drugs*. Hydrochlorothiazide 2. Amlodipine besilate 3. Valsartan 4. Atorvastatin calcium hydrate 5. Candesartan cilexetil Column: YMC-Triart C8 (3 µm, 2 nm), 5 x 2. mm ID Part No.: TO2S3-52WT Eluent: A) water / formic acid (/.) B) acetonitrile / formic acid (/.) Gradient: -9% B (-5 min), 9% B (5-7 min) Flow rate:.4 ml/min Temperature: 3 C Detection: UV at 254 nm Injection: 2 µl (-2 µg/ml) Sequential analysis of Carvedilol* Triart C8 Column: YMC-Triart C8 (5 µm, 2mm) 5 x 2. mm ID Part No.: TO2S5-52WT Eluent: phosphate buffer (ph 2.)* / acetonitrile (65/35) *Dissolve 2.72 g of KH 2 PO 4 in 9 ml water, adjust ph 2. with H 3 PO 4 and add water to make ml Flow rate:.28 ml/min (adjust the flow rate so that the retention time of carvedilol is about 4 min) Temperature: 55 C Detection: UV at 24 nm No change in retention time is observed even under a high ph and at an elevated temperature. 26

27 Pharmaceuticals Separation of alkaloids*. Scopolamine 2. Atropine 3. Cinchonine 4. Quinine 5. Dihydroquinine Column: YMC-Triart C8 (5 µm, 2 nm) 5 x 2. mm ID Part No.: TA2S5-52WT Eluent: 2 mm CH 3 COOH-CH 3 COONH 4 (ph 4.9) / acetonitrile (8/2) Flow rate:.2 ml/min Temperature: 4 C Detection: UV at 22 nm Injection: µl (.2-. mg/ml) Barbiturates in human serum* A) Standard ( µg/ml) Solid-phase extraction method YMC Dispo SPE C8 mg/ml Condition 2 ml methanol 2 ml water B) Spiked human serum Load 5 µl spiked human serum solution (each µg) Elute 5 µl methanol/water (85/5) Dilute 5 µl 2 mm ammonium formate buffer (ph 9.5) Column: YMC-Triart C8 (5 µm, 2 nm) 5 x 2. mm ID Part No.: TA2S5-52WT Eluent: A) 2 mm HCOONH 4 -NH 3 (ph 9.5) B) methanol Gradient: -9% B (-7 min) Flow rate:.2 ml/min Temperature: 25 C Detection: UV at 24 nm Injection: µl 27

28 Pharmaceuticals Erythromycin at elevated ph and temperature*. Optimisation of ph 2. Optimisation of temperature (ph 7.9) 3. Stability test: ph 7.9, 7 C Number of injections Column: YMC-Triart C8 (3 µm, 2 nm) 5 x 2. mm ID Part No.: TA2S3-52WT Eluent: 2 mm KH 2 PO 4 -K 2 HPO 4 / acetonitrile / methanol (4/45/5) Flow rate:.2 ml/min Detection: UV at 2 nm 28

29 Pharmaceuticals Separation of flavonoids* Column: YMC-Triart C8 (5 µm, 2 nm) 5 x 3. mm ID Part No.: TA2S5-53WT Eluent: acetonitrile / mm H 3 PO 4 (3/7) Flow rate:.425 ml/min Temperature: 37 C Detection: UV at 28 nm Injection: 2 µl (5 µg/ml) Separation of aromatic carboxylic acids* Column: YMC-Triart C8 (5 µm, 2 nm) 5 x 3. mm ID Part No.: TA2S5-53WT Eluent: mm CH 3 COOH-CH 3 COONH 4 (ph 4.2) / acetonitrile (75/25) Flow rate:.425 ml/min Temperature: 4 C Detection: UV at 254 nm Injection: 4 µl (.2 ~.3 mg/ml) 29

30 Pharmaceuticals Aciclovir syrup and injection* Column: YMC-Triart C8 (5 µm, 2 nm) 5 x 4.6 mm ID Part No.: TA2S5-546WT Eluent: phosphate buffer* / methanol (95/5) *Dissolve.45 g of H 3 PO 4 and 25 ml of mol/l CH 3 COOH in water to make 9 ml q adjust ph 2.5 by mol/l NaOH q add water to make ml Flow rate:. ml/min Temperature: 25 C Detection: UV at 254 nm Injection: 2 µl (.5 mg/ml,.32 mg/ml) Clindamycin hydrochloride* Column: YMC-Triart C8 (5 µm, 2 nm) 25 x 4.6 mm ID Part No.: TA2S5-2546WT Eluent: 5 mm KH 2 PO 4 (ph 7.5 adjusted by 8 M KOH) / acetonitrile (55/45) Flow rate:. ml/min Temperature: 25 C Detection: UV at 2 nm Injection: µl Analysis of amlodipine besilate* Column: YMC-Triart C8 (5 µm, 2 nm) 5 x 3. mm ID Part No.: TA2S5-53WT Eluent: mm CH 3 COOH-CH 3 COONH 4 (ph 4.2) / acetonitrile (75/25) Flow rate:.425 ml/min Temperature: 4 C Detection: UV at 254 nm Injection: 4 µl (.2 ~.3 mg/ml) 3

31 LC / MS LC / MS compatibility YMC-Triart C8 No column Column: 5 µm, 5 x 2. mm ID Part-No.: TA2S5-52WT Eluent: A) water +.% formic acid B) acetonitrile +.% formic acid Gradient: 5% B (- min), 5-% B (-5 min), % B (5- min), -5% B (-. min), 5% B (.-2.5 min) Flow rate:.4 ml/min Temperature: 4 C Detection: ESI positive, TIC (Mass Range: 5-) Column bleeding, caused by the fragments of stationary phase, is the main reason for background noise and restrictions on detection limits. No bleed is observed in the test of total ion current (TIC) measured by LC/MS with blank or with YMC-Triart C8. So in terms of the signal/noise ratio (S/N ratio), YMC-Triart C8 can be expect to not only reduce the background noise but to also increase the sensitivity of the analysis. LC / MS analysis of benzodiazepine derivates Peak : ng_ml_rb4 229.d: EIC 343.5±.+All MS Column: Peak 2: ng_ml_rb4 229.d: YMC-Triart C8 (5 µm, 2 EIC nm) ±.+All 5 x 2. mm ID MS Part-No.: TA2S5-52WT Eluent: A) mm formic acid B) acetonitrile Gradient: 25-5% B (- min) Flow rate:.2 ml/min Temperature: 4 C Detection: Bruker Daltonics microtof, ESI, positive mode Injection: 5 µl ( ng/ml) Courtesy of J. Watanabe, Bruker Daltonics K. K.,Application Data by courtesy YMC Co., Ltd. 3

32 LC / MS LC/MS analysis of tetracycline antibiotics* Column: YMC-Triart C8 (5 µm, 2 nm) 5 x 2. mm ID Part No.: TA2S5-52WT Eluent: acetonitrile / water / formic acid (5/85/.) Flow rate:.4 ml/min Temperature: 4 C Detection: A) UV at 27 nm B) ESI positive-mode Injection: µl ( µg/ml) 32

33 LC / MS Analysis of 36 pesticides in a single run Column: YMC-Triart C8 (3 µm, 2nm) x 2. mm ID Part No.: TA2S3-2WT Eluent: A) 5 mm ammonium formate / water B) 5 mm ammonium formate / methanol Gradient: min: 3% B,. min: 5% B, 8 min: % B, 2 min: % B, 2. min: 3% B, 29 min: 3% B Total run time: 3 min Flow rate:.25 ml/min Temperature: 45 C Injection: 5 µl Sample: ng/ml pesticide mix in acetonitrile Application data by courtesy of: József László WIREC, WESSLING International Research and Educational Centre Nonprofit Co. (Hungary) 33

34 Food Soy isoflavones in supplement* A) Standard (. mg/ml). Daidzin 2. Glycitin 3. Genistin O-Malonyldaidzin O-Malonylglycitin O-Acetyldaidzin O-Acetylglycitin O-Malonylgenistin 9. Daidzein. Glycitein. 6 -O-Acetylgenistin 2. Genistein Sample preparation method B) Supplement ( g/5 ml) Supplement 5% aqueous ethanol Stirred at r.t. for hr Filtration Injection Column: YMC-Triart C8 (3 µm, 2 nm) 5 x 2. mm ID Part No.: TA2S3-52WT Eluent: A) acetonitrile / water / HCOOH (/9/.) B) acetonitrile / water / HCOOH (6/4/.) Gradient: 5-4% B (-2 min) Flow rate:.4 ml/min Temperature: 25 C Detection: UV at 254 nm Injection: 2 µl Separation of water-soluble vitamins*. Thiamine HCl (Vitamin B ) 2. Pyridoxine HCl (Vitamin B 6 ) 3. Nicotinamide 4. Cyanocobalamin (Vitamin B 2 ) 5. L-Ascorbic acid 2-glucoside 6. L-Ascorbic acid (Vitamin C) 7. Erythorbic acid 8. Riboflavin (Vitamin B 2 ) 9. Nicotinic acid (Vitamin B 3 ) Column: YMC-Triart C8 (5 µm, 2 nm) 25 x 4.6 mm ID Part No.: TA2S5-2546WT Eluent: phosphate buffer* / acetonitrile (9/) * Dissolve.4 g KH 2 PO 4 in 8 ml water q add 26 ml % TBA OH q adjust ph 5.2 by 2% H 3 PO 4 q add water to make ml Flow rate:.8 ml/min Temperature: 4 C Detection: UV at 26 nm Injection: µl (5 µg/ml) 34

35 Food Analysis of anthocyanins and anthocyanidins Anthocyanins: Anthocyanidins: Indicated in black Indicated in blue. Delphinidin-3-O-galactoside 2. Delphinidin-3-O-glucoside 3. Cyanidin-3-O-galactoside 4. Delphinidin-3-O-arabinoside 5. Cyanidin-3-O-glucoside 6. Petunidin-3-O-galactoside 7. Cyanidin-3-O-arabinoside 8. Petunidin-3-O-glucoside 9. Peonidin-3-O-galactoside. Petunidin-3-O-arabinoside. Delphinidin 2. Peonidin-3-O-glucoside 3. Malvidin-3-O-galactoside 4. Peonidin-3-O-arabinoside 5. Malvidin-3-O-glucoside 6. Malvidin-3-O-arabinoside 7. Cyanidin 8. Petunidin 9. Peonidin 2. Malvidin Column: YMC-Triart C8 (5 µm, 2 nm) 25 x 4.6 mm ID Part No.: TA2S5-2546WT Eluent: A) water / formic acid (9/) B) acetonitrile / methanol / water / formic acid (22.5/22.5/4/) Gradient: 2-28% B (-3 min), 28-7% B (3-4 min), % B (4-45 min) Flow rate:. ml/min Temperature: 25 C Detection: UV/VIS at 535 nm Sample: commercial bilberry powder (.25 mg/ml) 35

36 Life Science Proteins High sensitivity and sharp peaks under LC/MS compatible conditions* YMC-Triart Bio C4 (3 μm, 3 nm) XBridge Protein BEH C4 (3.5 μm, 3 nm) Sharper Peaks! AdvanceBio RP-mAb C4 (3.5 μm, 45 nm) Aeris widepore C4 (3.6 μm, 2 nm) 6: no elution, 5, 6: no elution Column: 5 x 3. mm ID Eluent: A) water/formic acid (/.) B) acetonitrile/formic acid (/.) Gradient: -95%B (-5 min) Temperature: 4 C Detection: UV at 22 nm Sample:. Cytochrome-C (Horse heart) 2. Insulin (Bovine pancreas) 3. Transferrin (Human) 4. BSA 5. β-lactoglobulin (Bovine) 6. α-chymotrypsinogen A (Bovine pancreas) YMC-Triart Bio C4 shows better peak shape and recovery with a mobile phase containing formic acid, which is commonly used for LC/MS analysis. Therefore, YMC-Triart Bio C4 is ideal for highly sensitive analysis of proteins. 36

37 Life Science Proteins Ideal for Microanalysis* YMC-Triart Bio C4 (.9 μm, 3 nm) Insulin ng 2 ng ng BSA 5 ng ng 5 ng Lactoferrin 5 ng ng 5 ng Ovalbumin 5 ng ng 5 ng Aeris widepore C4 (3.6 μm, 2 nm) Insulin ng 2 ng ng BSA 5 ng ng 5 ng Lactoferrin 5 ng ng 5 ng Ovalbumin 5 ng ng 5 ng Column: 5 x 2. mm ID Eluent: A) water/tfa (/.5) B) acetonitrile/tfa (/.5) Gradient 25-6%B (-5 min), 9%(5-2 min), 25%(2-35 min) Detection: UV at 22 nm Temperature: 4 C No adsorption was observed on YMC-Triart Bio C4 even at a low loading amount. This feature is beneficial for microanalysis of proteins. Amyloid β-proteins mau. Amyloid β (-38) (Human) (MW 432) 2. Amyloid β (-4) (Human) (MW 433) 3. Amyloid β (-42) (Human) (MW 454) 4. Amyloid β (-43) (Human) (MW 465) min Amyloid β (-43) : Asp-Ala-Glu-Phe-Arg-His-Asp-Ser-Gly-Tyr-Glu-Val-His-His-Gln-Lys-Leu-Val-Phe-Phe- Ala-Glu-Asp-Val-Gly-Ser-Asn-Lys-Gly-Ala-Ile-Ile-Gly-Leu-Met-Val-Gly-Gly-Val-Val-Ile-Ala-Thr Column: YMC-Triart Bio C4 (3 μm, 3 nm) 5 x 3. mm ID Part No.: TB3S3-53PTH Eluent: A) water/tfa (/.) B) acetonitrile/tfa (/.) Gradient: 25-4%B (-3 min), 9%B (3-4 min) Flow rate:.4 ml/min Temperature: 7 C Detection: UV at 22 nm Injection: 4 μl (each. mg/ml) 37

38 Life Science Proteins Proteins (MW 5,7 ~ 66,) mau 8 3. Ribonuclease A (Bovine pancreas) (MW 3,7) 2. Cytochrome-C (Horse heart) (MW 2,4) 3. Insulin (Bovine pancreas) (MW 5,7) 4. BSA (MW 66,) 5. α-chymotrypsinogen A (Bovine pancreas) (MW 25,7) min Column: YMC-Triart Bio C4 (5 μm, 3 nm) 5 x 3. mm ID Part No.: TB3S3-53PTH Eluent: A) water/tfa (/.) B) acetonitrile/tfa (/.) Gradient: 2-6%B (-27 min), 9%B (27-35 min) Flow rate:.4 ml/min Temperature: 7 C Detection: UV at 22 nm Injection: μl (.25 ~.5 mg/ml) Proteins (MW 4,3 ~ 77,) mau 8. Lysozyme (Chicken egg white) (MW 4,3) 2. Conalbumin (Chicken egg white) (MW 77,) 3. β-lactoglobulin A (Bovine milk) (MW 8,4) 4. Ovalbumin (MW 45,) min Column: YMC-Triart Bio C4 (5 μm, 3 nm) 5 x 3. mm ID Part No.: TB3S3-53PTH Eluent: A) water/tfa (/.) B) acetonitrile/tfa (/.) Gradient: 2-6%B (-27 min), 9%B (27-35 min) Flow rate:.4 ml/min Temperature: 7 C Detection: UV at 22 nm Injection: μl (.25 ~.5 mg/ml) 38

39 Life Science Proteins Highly efficient RP-HPLC separation of proteins and peptides using high temperature* Mixture A (MW 5 8,4) Analytes MW Peak width /2 (min) 4 C 7 C Mixture A. Oxytocin, Leu-Enkephalin b-endorphin 3,465.6 Mixture B (MW 4,3 25,7) 4. Insulin 5, b-lactoglobulin A 8, Mixture B. Lysozyme 4, a-chymotrypsinogen 25, b-lactoglobulin A 8, Column: YMC-Triart C8 (.9 µm, 2 nm) 5 x 2. mm ID Part-No.: TA2SP9-52WT Eluent: A) water / TFA (/.) B) acetonitrile / TFA (/.) - mixture A B) acetonitrile / 2-propanol / TFA (5/5/.) - mixture B Gradient: -8% B (-5 min) - mixture A 3-6% B (-5 min) - mixture B Flow rate: Detection: Injection: System:.4 ml/min UV at 22 nm µl (5 µg/ml) - condition A µl (25 µg/ml) - condition B Agilent 2SL PC (peak capacity) = + (gradient time / peak width*) *peak width = 2W.5h average 39

40 Triart Life Science Antibodies Adalimumab (MW: ca. 48 kda)* mau mau min* min* Bevacizumab (MW: ca. 48 kda)* mau Column: Part No.: Eluent: Gradient: 4 4 YMC-Triart Bio C4 (3 µm, 3 nm) 5 x 3. mm ID TB3S3-53PTH A) water/tfa (/.) B) acetonitrile/tfa (/.) Time [min] Eluent B [%] Flow rate: Temperature: Detection: Injection:.4 ml/min 25 C UV at 22 nmn 4 μl (.5 mg/ml) min*

41 Life Science Peptides Peptides (MW 556-3,465)*. Oxytocin 2. Met-Enkephalin 3. Leu-Enkephalin 4. Neurotensin 5. γ-endorphin 6. β-endorphin (MW,7) (MW 574) (MW 556) (MW,673) (MW,859) (MW 3,465) Column: YMC-Triart C8 (5 µm, 2 nm) 5 x 2. mm ID Part No.: TA2S5-52WT Eluent: A) water +.% TFA B) acetonitrile +.% TFA Gradient: 2-45% B (-25 min) Flow rate:.2 ml/min Temperature: 37 C Detection: UV at 22 nm Injection: 2 ml (.75 ~.25 mg/ml) 4

42 Life Science Peptides / Amino Acids Peptide mapping* mau Peak capacity: PC (peak capacity) = + (gradient time / peak width*) *peak width = 2W.5h average min Column: YMC-Triart C8 (.9 μm, 2 nm) 2 x 2. mm ID (Two coupled x 2. mm ID) Part No.: TA2SP9-2PT (2x) Eluent: A) water/tfa (/.) B) acetonitrile/tfa (/.8) Gradient: 5-4%B (-3 min) Flow rate:.4 ml/min Temperature: 7 C Detection: UV at 22 nm Injection: 2 μl Sample: Triptic digest of Bovine Hemoglobin (2.5 nmol/ml) Pressure: MPa (8,43-8,93 psi) Amino acids with % aqueous phase mau L-aspartic acid 3 L-histidine L-arginine 4 L-methionine min Column: YMC-Triart C8 (.9 μm, 2 nm) 5 x 3. mm ID Part No.: TA2SP9-53PT Eluent: 4 mm K 2 HPO 4 (ph 7.) Flow rate:.3 ml/min Temperature: 2 C Detection: UV at 2 nm Injection: 2 μl ( mg/ml) 42

43 Life Science Amino Acids Free amino acids in HILIC mode* pa * Chloride ion contained in the sample solution ** Sodium ion contained in the sample solution 5 * * * * ** Phenylalanine (Phe) Tryptophan (Trp) Leucine (Leu) Isoleucine (Ile) Methionine (Met) Tyrosine (Tyr) Valine (Val) Cysteine HCl (Cys) Proline (Pro) Threonine (Thr) Alanine (Ala) -Aminobutyric acid (GABA) Serine (Ser) Glycine (Gly) Asparagine (Asn) Glutamine (Gln) Citrulline (Cit) Glutamic acid (Glu) Histidine (His) Aspartic acid (Asp) Arginine HCl (Arg) Lysine (Lys) Ornithine HCl (Orn) min Column: YMC-Triart Diol-HILIC (5 μm, 2 nm) 5 x 4.6 mm ID Part No.: TDH2S5-546PTH Eluent: A) mm HCOOH-HCOONH 4 (ph 3.6) B) acetonitrile Gradient: 83-8%B (-2 min), 8-68%B (2-2 min) Flow rate:. ml/min Temperature: 4 C Detection: Corona CAD (Charged Aerosol Detector) Injection: μl (. mg/ml) Corona and CAD are trademarks of Thermo Fisher Scientific. 43

44 Life Science Oligonucleotides Oligonucleotides d(t)2-2 method transfer from HPLC to UHPLC* Column: Part No. Flow rate: Gradient: YMC-Triart C8 (5 µm, 2 nm) 5 x 2. mm ID TA2S5-52WT.2 ml/min 3-5% B (-84 min) 8 min x faster Column: Part No. Flow rate: Gradient: YMC-Triart C8 (.9 µm, 2 nm) 5 x 2. mm ID TA2SP9-52PT.8 ml/min 35-5% B (-7 min) 7 min Eluent: A) mm di-n-butylamine-acetic acid (ph 6.) B) methanol Detection: UV at 269 nm Injection: µl (5 nmol/ml) Temperature: 37 C Synthetic oligonucleotides mau min*. 5 -CAC UGA AUA CCA AU-3 (4mer) UCA CAC UGA AUA CCA AU-3 (7mer) UCA UCA CAC UGA AUA CCA AU-3 (2mer) GUC AUC ACA CUG AAU ACC AAU-3 (2mer) Column: Eluent: Gradient: YMC-Triart C8 (.9 µm, 2 nm) 5 x 2. mm ID A) 2 mm HFIP*-8 mm triethylamine B) methanol -2%B (- min) *hexafluoroisopropanol Flow rate:.42 ml/min Temperature: 65 C Detection: UV at 26 nm Injection: µl (2-4 nmol/ml) Courtesy of M. Yamada, SHIMADZU CORPORATION 44

45 Life Science Oligonucleotides LC/MS analysis of mirna 5 -pugg AGU GUG ACA AUG GUG UUG-3 (2 nt, MW 689.) 5 -pugg AGU GUG ACA AUG GUG UUG U-3 (22 nt, MW 796.3) Column: YMC-Triart C8 (3 µm, 2 nm), 5 x 2. mm ID Part No.: TA2S3-52WT Eluent: A) mm di-n-butylamine-acetic acid (ph 7.5) B) mm di-n-butylamine-acetic acid (ph 7.5)/acetonitrile (5/5) Gradient: 62-72%B (-2 min) Flow rate:.2 ml/min Temperature: 3ºC Detection: A) UV at 26 nm B) ESI-negative mode Injection: 4 µl (5 nmol/ml) System: LC) Shimadzu Prominence MS) Shimadzu LCMS22 Courtesy of M. Yamada, SHIMADZU CORPORATION Effect of mobile phase and column temperature on separation of sirna duplex* Crude synthetic sirna duplex (9 bp): 5 -CGU ACG CGG AAU ACU UCG AdTdT-3 3 -dtdtgca UGC GCC UUA UGA AGC U-5 Column: YMC-Triart C8 (.9 µm, 2 nm) x 2. mm ID Part No.: TA2SP9-2PT Flow rate:.2 ml/min Detection: UV at 269 nm Injection: µl (5 nmol/ml) System: Agilent 29 Condition A Eluent: A) mm di-n-butylamine-acetic acid (ph 6.) B) methanol Gradient: 35-6%B (-5 min) Condition B Eluent: A) 2 mm triethylamine-acetic acid (ph 7.) B) acetonitrile Gradient: 5-2%B (-2 min) 45

46 YMC-Triart "AQ" YMC-Triart C8 General The use of % water eluent has been a challenge in HPLC analysis for decades. Even today, many C8 materials suffer from unacceptable short lifetime, because the C8 chains collapse and this reduces the separation performance drastically. As a pioneer in this field, YMC has offered a product as early as the 8 s, which presents a synonym for stability under aqueous conditions: YMC-Pack ODS-AQ. AQ -type phases are particularly suitable for the separation of polar substances, metabolites, pesticides, degradation products, peptides and protein digests YMC-Pack ODS-AQ hydrophilic C8 balanced surface chemistry polar recognition metabolite recognition 5 µm 3 µm 46

47 23 Hydrosphere C8 A new, ultrapure silica base was introduced whilst adapting the surface chemistry to maintain the AQ -type properties. hydrophilic C8 surface for enhanced polar recognition stable when used with % aqueous eluent no need for ion pair reagents addition of 2 µm particle size for Fast-LC (YMC-Pack UltraHT) 5 µm 3 µm 2 µm YMC-Triart "AQ" = YMC-Triart C8 The latest YMC technology platform consists of a hybridstyle substrate with enhanced stability against ph -2 temp. up to 9 C % H 2 O 5 µm 3 µm.9 µm Therefore, YMC-Triart provides freedom in method development, robustness and enhanced column lifetimes for reproducible results, day-after-day, year-afteryear. YMC-Triart is fully scalable within (U)HPLC HPLC with its micron particle sizes in order to facilitate lab-to-lab method transfer. 47

48 YMC-Triart "AQ" YMC-Triart C8 Problem with conventional C8 columns Aqueous conditions deteriorate column performance*. Cytosine 2. Uracil 3. Guanine 4. Thymine 5. Adenine Example Example 2 Before pump stopped tr 5 = tr 5 = 4 5 After pump stopped 63.5% of initial retention 82.2% of initial retention 5 5 min 5 5 min Column: 5 µm, 5 x 4.6 mm ID XBridge C8 (Example ) and Atlantis T3 (Example 2) Eluent: 2 mm KH 2 PO 4 -K 2 HPO 4 (ph 6.9) Flow rate:. ml/min Temperature: 37 C Detection: UV at 254 nm Why? The columns used for applications involving % aqueous buffers provide shorter retention times after the flow was stopped between analyses. This behaviour is caused by poor hydration of the phase. Polar compounds cannot easily distribute between the mobile phase and the stationary phase. Image of C8 surface hydration 48

49 YMC-Triart "AQ" YMC-Triart C8 Triart YMC-Triart "AQ" YMC-Triart C8 Solution with YMC-Triart C8 Reproducible stable performance!* No retention time changes! Before pump stopped tr 5 = 4 5 After pump stopped 98.% of initial retention 5 5 min Column: YMC-Triart C8 (5 µm, 2 nm) 5 x 4.6 mm ID Part No.: TA2S5-546WT Eluent: 2 mm KH 2 PO 4 -K 2 HPO 4 (ph 6.9) Flow rate:. ml/min Temperature: 37 C Detection: UV at 254 nm When YMC-Triart C8 columns are used for applications involving % aqueous buffers, the retention times are unchanged after the flow was stopped between analyses. This is due to the improved hydration of the phase. Polar compounds can easily distribute between the mobile phase and the stationary phase. Image of C8 surface hydration 49

50 YMC-Triart "AQ" YMC-Triart C8 From the inventors of AQ-columns: YMC-Triart C8 "validated" for AQ-conditions!* HPLC 5 µm, 25 x 3. mm ID 2, ml/min 4 N(4)=229 8 MPa. Oxalic acid 2. Tartaric acid 3. Glycolic acid 4. Formic acid 5. L-Malic acid 6. Malonic acid 7. Lactic acid 8. Acetic acid 9. Maleic acid. Citric acid. Succinic acid 2. Fumaric acid 3. Acrylic acid 4. Propionic acid min 2 min UHPLC.9 µm, x 3. mm ID 2,3.85 ml/min N(4)=5 54 MPa 4 min min Column: YMC-Triart C8 Part No.: TA2S5-253WT / TA2SP9-3PT Eluent: 2 mm H 3 PO 4 Flow rate:.425 ml/min Temperature: 37 C Detection: 22 nm% aqueous conditions! Stable under harsh conditions: ph -2 and temperature up to 9 C. Stable retention times with % aqueous eluents! Reproducible results day-after-day, column-to-column and lab-to-lab! 5

51 YMC-Triart "AQ" YMC-Triart C8 Proven reliability* th 4 th (%) 2 Retention stability of Adenine Adenine 3 th th 4 th 2 st min Column: YMC-Triart C8 (5 µm, 2 nm) 5 x 3. mm ID Part No.: TA2S5-53WT Eluent: 2 mm KH 2 PO 4 -K 2 HPO 4 (ph 6.9) Flow rate:.425 ml/min Temperature: 37 C Detection: UV at 254 nm Sample:. Cytosine 2. Uracil 3. Guanine 4. Thymine 5. Adenine Analysis number No change is found in the separation parameters including retention times, even after 5 injections when using YMC-Triart C8. YMC-Triart C8 Hydrosphere C min min. L-Ascorbic acid 2-glucoside 2. L-Ascorbic acid (Vitamin C) 3. D-Isoascorbic acid 4. Nicotinic acid (Vitamin B 3 ) 5. Nicotinamide 6. D-(+)-Pantothenic acid calcium salt (Vitamin B 5 ) 7. Pyridoxal hydrochloride (Vitamin B 6 ) 8. Pyridoxine hydrochloride (Vitamin B 6 ) 9. Folic acid (Vitamin B 9 ). Thiamine hydrochloride (Vitamin B ). Cyanocobalamin (Vitamin B 2 ) 2. D-Biotin (Vitamin B 7 ) 3. Riboflavin (Vitamin B 2 ) YMC-Pack ODS-AQ , min Column: 3 µm, 5 x 3. mm ID Part No.: TA2S5-53WT Eluent: A) 2 mm KH 2 PO 4 -H 3 PO 4 (ph 2.8) containing 5 mm CH 3 (CH 2 ) 5 SO 3 Na B) 2 mm KH 2 PO 4 -H 3 PO 4 (ph 2.8) / acetonitrile (8/2) containing 5 mm CH 3 (CH 2 ) 5 SO 3 Na Flow rate:.425 ml/min Temperature: 4 C Detection: 2 nm Retention behaviour of water-soluble vitamins on three YMC ODS phases which can be used with % aqueous mobile phases is compared. The retention times and peak elution order for folic acid (peak 9), thiamine hydrochloride (peak ) and cyanocobalamin (peak ) are different for the three phases due to the balance of hydrophobicity and hydrogen bonding capacity differing between the three phases. 5

52 HILIC Great stability and reproducibility at high ph* Stability at high ph (ph, 5 C)** % Triart Diol-HILIC Cytosine 2 hr Rate on N [%] 8% 6% 4% 5 hr hr 2% Silica based Diol 5 hr % ** ph < is recommended for regular use Time [h] Column: 5 µm, 5 x 4.6 mm ID Part No.: TDH2S5-546WT Eluent: acetonitrile / water / NH 3 (9//.) ph.3 Flow rate:. ml/min Temperature: 5 C Sample: Cytosine min initial YMC-Triart Diol-HILIC offers highly reproducible separations even at high ph and high temperature. The lifetime of YMC-Triart Diol-HILIC is much longer than that of conventional silica-based Diol columns. Nucleosides and bases* mau Uracil 2. Uridine 3. Adenosine 4. Cytosine 5. Cytidine 6. Guanosine Diabetes drugs* mau NH NH H N NH NH 2 H Cl Phenformin hydrochloride NH NH H 2 N NH N CH 3 HCl CH 3 Metformin hydrochloride 5 5 V25A min U2529D min Column: YMC-Triart Diol-HILIC (5 µm, 2 nm) 5 x 3. mm ID Part No.: TDH2S5-53WT Eluent: mm CH 3 COONH 4 / acetonitrile (/9) Flow rate:.425 ml/min Temperature: 3 C Detection: UV at 254 nm Injection: 2 µl (5 ~ µg/ml) Column: YMC-Triart Diol-HILIC (.9 µm, 2 nm) 5 x 2. mm ID Part No.: TDH2SP9-52PT Eluent: mm HCOOH-HCOONH 4 (ph 3.7) / acetonitrile (/9) Flow rate:.8 ml/min Temperature: 25 C Detection: UV at 235 nm Injection: 2 µl ( µg/ml) 52

53 HILIC Water soluble vitamins*. Caffeine 2. Nicotinamide 3. Pyridoxine hydrochloride (Vitamin B 6 ) 4. Riboflavin (Vitamin B 2 ) 5. Orotic acid 6. Erythorbic acid (D-Isoascorbic acid) 7. L-Ascorbic acid (Vitamin C) 8. Nicotinic acid (Vitamin B 3 ) 9. 2-O-a-D-Glucopyranosyl-L-ascorbid acid (Ascorbic acid 2-glycoside). Thiamine hydrochloride (Vitamin B ). Cyanocobalamin (Vitamin B 2 ) Column: YMC-Triart Diol-HILIC (5 µm, 2 nm) 5 x 3. mm ID Part No.: TDH2S5-53PTH Eluent: A) acetonitrile / 2 mm HCOOH-HCOONH4 (ph 3.6) / water (9/5/5) B) acetonitrile / 2 mm HCOOH-HCOONH4 (ph 3.6) / water (5/5/45) Gradient: -75% B (-2 min) Flow rate:.425 ml/min Temperature: 4 C Detection: UV at 254 nm Injection: 4 µl (5 µg/ml) Polar and hydrophilic compounds* mv 2 RP mode YMC-Triart C8 2 mm KH 2 PO 4 -H 3 PO 4 (ph 2.5) 2 HO HO H O O HO OH Erythorbic acid (D-Isoascorbic acid) mv 2 HILIC mode YMC-Triart Diol-HILIC mm HCOOH-HCOONH 4 (ph 3.3) / acetonitrile (/9) 2 HO HO H O O 2 F2425A min HO OH L-Ascorbic acid B2427L min Column: 5 µm, 5 x 3. mm ID Flow rate:.425 ml/min Temperature: 4 C Detection: UV at 254 nm Injection: 4 µl (.5 mg/ml) YMC-Triart C8 (RP) shows very weak retention and poor resolution of L-ascorbic acid and its stereoisomer (erythorbic acid) even if % aqueous mobile phase is used. However, YMC-Triart Diol-HILIC shows strong retention and good resolution of these compounds with mobile phase containing 9% organic solvent. 53

54 QC Data YMC-Triart: Improved quality of particles Uniform spherical particles YMC-Triart X-Bridge HILIC by courtesy of YMC Co., Ltd. The uniform spherical particle support is used for all YMC-Triart phases. The particles are produced using micro-reactor technology for the granulation process. This results in reduction of the back-pressure and leads to more reproducibility in surface modification. Low column back-pressure* YMC-Pack Pro C8, methanol/h 2 O YMC-Pack Pro C8, acetonitrile/h 2 O YMC-Triart C8, methanol/h 2 O YMC-Triart C8, acetonitrile/h 2 O Column: YMC-Triart C8 (5 µm) 5 x 4.6 mm ID Part No.: TA2S5-546WT Eluent: acetonitrile / water or methanol / water Flow rate:. ml/min Temperature: 25 C The revolutionary production technique, adapted from micro-reactor flow technology, produces a silica/organic hybrid stationary phase, with outstanding narrow pore size and particle size distri-butions which result in low back pressures. YMC-Triart is designed for use under a wide range of conditions. Elution with higher viscosity methanol (compared with acetonitrile), YMC-Triart generates lower pressure (approx. 3% lower than with conventional phases). 54

55 QC Data Batch-to-batch reproducibility E xcellent reproducibility of YMC-Triart phases is available even for the analysis of basic and coordination Basic compounds* compounds which normally exhibit tailing and adsorption effects. Coordination compounds* 2 3. Chlorpheniramine (basic) 2. Dextromethorphan (basic) 3. Propyl p-hydroxybenzoate 2. Hinokitiol (coordination) 2. Methyl benzoate Lot. E Lot. E Lot. D Lot. D Lot. C Lot. C Lot. B Lot. B. 2.5 J92Y min Lot. A. 2. J92X min Lot. A Column: YMC-Triart C8 (5 µm) 5 x 3. mm ID Part No.: TA2S5-53WT Eluent: 2 mm KH 2 PO 4 (ph 6.9) / acetonitrile (65/35) Flow rate:.425 ml/min Temperature: 4 C Detection: UV at 235 nm Column: YMC-Triart C8 (5 µm) 5 x 3. mm ID Part No.: TA2S5-53WT Eluent: acetonitrile /.% H 3 PO 4 (4/6) Flow rate:.425 ml/min Temperature: 4 C Detection: UV at 254 nm The reproducibility of packed columns is shown below in terms of theoretical plate number (N) and tailing factor (Tf). YMC-Triart packed columns exhibit a very narrow range of variation Theoretical plate number 5 5 Theoretical plate number Tailing factor Tailing factor Column number.5 Column: YMC-Triart C8 (5 µm) 5 x 4.6 mm ID Part No.: TA2S5-546WT Eluent: acetonitrile / water (4/6) Flow rate:. ml/min Temperature: ambient Sample: butyl benzoate 55

56 QC Data Narrow pore distribution 45 d Vp/ d logd YMC-Triart C8 particle Conventional silica-based particle X-Bridge HILIC Pore size (nm) This figure shows the pore size distributions of some competitive material. Comparing the pore size distributions shows that YMC-Triart has a narrower distribution which results in sharper peak shapes. Improved loadability Influence of injection volume on peak shapes 2 YMC-Triart C8 solvent for sample acetonitrile injection volume. µl.5 µl 2. µl 3. µl Hybrid-silica based C8 2 2 Silica based C8. OCH 2 CHCH 2 NHCH(CH 3 ) 2 OH Peak anomaly at.5 µl Propranolol (5 µg/ml) 2. H 3 CO CN CH 3 OCH min H 3 CO C (CH 2 ) 3 NCH 2 CH 2 CH(CH 3 ) 2 CH 3 Verapamil (5 µg/ml) OCH 3 Column: Eluent: Gradient: Flow rate: Temperature: 4 C Detection: UV at 275 nm 5 µm, 5 x 2. or 2. mm ID A) water +.% formic acid B) acetonitrile +.% formic acid 5% B (-.5 min), 5-% B ( min).4 ml/min In order to prevent peak errors, there is a limit to the injection volume when a sample is injected in high elution solvents (such as % acetonitrile). Compared with traditional columns, more than double the injection volume can be injected into YMC-Triart columns as a result of the extremely narrow particle size distribution. 56

57 QC Data Multi-stage endcapping After bonding the alkyl chain, there are highly reactive and less reactive silanols on the surface. In traditional bonding processes, these are reacted with a single endcapping-compound in one step. However, the highly reactive silanols can be hydrolysed easily which contributes to the poor stability. The less reactive silanols are hard to endcap which Before EC results in poor resolution due to peak tailing. YMC-Triart phases use an innovation in endcapping called multistage endcapping for its surface modification process. By using a number of compounds with different reactivities in successive steps, all silanols can be capped to the maximum extent. STEP Hydroxyl group Endcapping (EC) group 2 STEP 3 STEP The chromatographic result of a good endcapping is demonstrated: Endcapping by traditional method Peak tailing for basic compounds YMC-Triart C8 Ingredients in cough/cold medication:. Chlorpheniramine 2. Dextromethorphan 3. Propyl p-hydroxybenzoate Column: 5 µm, 5 x 3. mm ID Part No.: TA2S5-53WT Eluent: 2 mm KH 2 PO 4 -K 2 HPO 4 (ph 6.9) / acetonitrile (65/35) Flow rate:.425 ml/min Temperature: 4 C Detection: UV at 235 nm 57

58 QC Data Basic compounds* YMC-Triart C8 5 x 3. mm ID silica based C8 Inertsil 5 x 4.6 mm ID hybrid-silica based C8 XBridge 5 x 4.6 mm ID Ingredients in a cough/cold medication. Chlorpheniramine 2. Dextromethorphan 3. Propyl p-hydroxybenzoate excellent peak shape Column: 5 µm, 5 x 3. or 5 x 4.6 mm ID Eluent: 2 mm KH 2 PO 4 -K 2 HPO 4 (ph 6.9) / acetonitrile (65/35) Flow rate:.425 ml/min for 3. mm ID. ml/min for 4.6 mm ID Temperature: 4 C Detection: UV at 235 nm The innovative surface modification technology results in excellent peak shapes even for basic compounds that often exhibit peak tailing with conventional silica- and hybrid silica-based reversed phase columns. Acidic compounds* YMC-Triart C8 5 x 3. mm ID Organic acid. Formic acid 2. Acetic acid 3. Propionic acid hybrid-silica based C8 Gemini NX 5 x 4.6 mm ID Column: 5 µm, 5 x 3. or 5 x 4.6 mm ID Eluent: methanol / water +.% H 3 PO 4 (5/95) Flow rate:.425 ml/min for 3. mm ID. ml/min for 4.6 mm ID Temperature: 37 C Detection: UV at 2 nm YMC-Triart phases are synthesised using methodology adapted from micro-reactor technology. This technique ensures a reduction of impurities that contribute to peak tailing during the analysis of some types acidic compounds. Coordinating compounds* YMC-Triart C8 5 x 3. mm ID hybrid-silica based C8 Gemini NX 5 x 4.6 mm ID Hinokitiol. Hinokitiol 2. Methyl benzoate low metal impurities Column: 5 µm, 5 x 3. or 5 x 4.6 mm ID Eluent: acetonitrile / water +.% H 3 PO 4 (4/6) Flow rate:.425 ml/min for 3. mm ID. ml/min for 4.6 mm ID Temperature: 4 C Detection: UV at 254 nm YMC-Triart phases have an extremely low level of metal impurities, much lower than conventional products, ensuring excellent peak shape for coordination compounds. 58

59 QC Data Comparison of clemastine analysis* mm phosphate buffer (ph 6.7)/organic solvent mm CH3COONH4/organic solvent methanol acetonitrile methanol acetonitrile YMC-Triart C8 5 x 2. mm ID Tf.97 Tf. Tf.3 Tf.6 F988H7 F988I7 F988F7 F987G7 Silica based C8 Inertsil ODS-3V 5 x 2. mm ID Tf.2 F9727H7 Tf.56 F9727I7 Tf.3 F9727F7 Tf.43 F983G7 Silica based C8 Capcell Pak MG-II 5 x 2. mm ID Tf.93 Tf 2.83 Tf.5 Tf 2.2 F977H7 F977I7 F974F7 F974G7 Silica based C8 Sunfire C8 5 x 2. mm ID Tf.3 Tf 3.87 Tf.3 Tf.88 F9722H7 F9722I7 F9722F7 F9722G7 Hybrid-silica based C8 X-Bridge 5 x 2. mm ID Tf.4 F973H7 Tf.88 F973I7 Tf.22 F973F min min min Tf.78 F973G7 8. min Column: 5 µm, 5 x 2. or 5 x 2. mm ID Eluent: A) mm KH 2 PO 4 -K 2 HPO 4 (ph 6.7) or mm CH 3 COONH 4 B) methanol or acetonitrile Gradient: 5-9% B (- min), 9% B (-5 min) Flow rate:.2 ml/min Temperature: 25 C Detection: UV at 23 nm Clemastine is a well-known basic compound which readily exhibits peak tailing with conventional ODS columns. YMC-Triart C8 provides sharp separations with many different buffer/solvent compositions. 59

60 Specifications Column hardware for bioseparations and coordinating compounds Obtain excellent resolution and great sensitivity No carry-over effects Reproducible results day-after-day! Specifications YMC-Triart Modifications C8, C8 ExRS, C8, Bio C4, Phenyl, PFP, Diol-HILIC Particle Size.9, 3, 5 µm Inner layer PEEK Outer layer Stainless steel Frit PEEK Pressure limit.9 µm: MPa (5, psi) 3/5 µm: 45 MPa (6,525 psi) Special column connectors required. See below for recommendations. Metal coordinating compounds, which have a phosphate group in their structure, tend to show poor peak shape due to interactions with metals, such as the stainless steel in column bodies and frits. By using the metal-free column hardware, better peak shapes can be expected. Nucleotides with phosphate groups also show better peak shapes when compared to the regular column hardware. The YMC-Triart metal-free column hardware is very suitable for highly sensitive analyses using LC/MS. Improved sensitivity for coordination compounds*. ATP 2. ADP 3. AMP mau Metal-free column mau Standard column 8 6 Tf()=.7 Tf(2)=.5 Tf(3)=. 8 6 Tf()=2.6 Tf(2)=2.5 Tf(3)= F6226 A 23 4m in F6226 C 23 4m in Column: YMC-Triart C8 (3 µm) 5 x 2. mm ID Part Nos.: TA2S3-5QPTP (metal-free) or TA2S3-5QPTH (regular hardware) Eluent: 5 mm HCOONH 4 Flow rate:.2 ml/min Temperature: 25 C Detection: UV at 265 nm Injection: µl ( mg/ml) 6

61 Column hardware for bioseparations and coordinating compounds Improved LC/MS results* Intensity cps Metal-free column Good Peak Shape! High Sensitivity! 2 3 Intensity cps Standard column Q/Q3 : 722/334 Q/Q3 : 76/336 Q/Q3 : 76/ min Blank run after the analysis of fumonisins standard min Intensity cps No Carry Over! Intensity cps min min =coordinating groups Column: YMC-Triart C8 (3 µm, 2 nm) 5 x 2. mm ID Part No.: TA2S3-5QPTP Eluent: A) water/hcooh (/.) B) acetonitrile 25-5% B (-5 min), 5% B (5-8 min), 5-9% B (8- min) Flow rate:.2 ml/min Temperature: 4 C Detection: ESI, positive Scheduld MRM (Metal free column) MRM (Standard column) Injection: 5 µl (. mg/ml) Instrument: LC) Shimadzu Prominence UFLC, MS) AB Sciex 32 QTRAP The YMC-Triart metal-free column showed excellent peak shapes when used to analyse fumonisins, while the regular column showed severe peak tailing due to interactions between the sample and the hardware. No carry over was observed when using the metal-free column, while the regular column showed sample carry over caused by adsorption of the sample on the hardware. The YMC-Triart metal-free column gives excellent peak shape for these coordination compounds and contributes to reliable analysis. Column connectors Recommendation Ferrule no replaceable Product MarvelX MarvelXACT Handy connector 2 Hand-tight EXP fitting Manufacturer IDEX Health & Science LLC IDEX Health & Science LLC YMC Co., Ltd. Optimize Technologies, Inc. Pressure rating 3 MPa /,3 bar 3 MPa /,3 bar 42 MPa / 42 bar 37 MPa /,37 bar Product code e.g. UPFP-6525 e.g. UPFP-YM7525 XRP24 XRHTF- MarvelX (ACT) is a registered trademark of IDEX Health & Science LLC EXP is a registered trademark of Optimize Technologies, Inc. 6

62 Substance index A Acesulfame K 4-Acetamidoacetophenone 5 2-Acetamidophenol 5, 6 Acetaminophene 6 Acetaminophenone 5 Acetanilide 5, 6 Acetic acid 5, O-Acetyldaidzin O-Acetylgenistin O-Acetylglycitin 34 Acetylsalicylic acid 6 Aciclovir 3 Acidic compounds 5, 58 Acrylic acid 5 Adalimumab 4 Adenine 48, 49, 5 Adenosine 48, 52 Adenosine diphosphate (ADP) 6 Adenosine monophosphate (AMP) 6 Adenosine triphosphate (ATP) 6 Adrenaline hydrochloride (A) 25 Alanine (Ala) 43 Alkaloids 29 4-Aminophenol 5 4-Aminophenone 6 γ-aminobutyric acid (GABA) 43 Amitriptyline 8, 23 Amlodipine besilate 26, 3 Amyloid β 37 Angiotensin I 2 Angiotensin II 2 Angiotensin III 2 Anthocyanidins 35 Anthocyanins 35 Arachidonic acid 9 L-Arginine (Arg) 42, 43 Artificial sweeteners L-Ascorbic acid 34, 5, 53 L-Ascorbic acid 2-glucoside 34, 5, 53 Asparagine (Asn) 43 L-Aspartic acid (Asp) 42, 43 Asulam 9 Atenolol 3 Atorvastatin calcium hydrate 26 Atropine 27 Azoxystrobin 9 B Baicalein 29 Barbital 27 Barbiturates 27 Basic compounds 58 Benzethonium chloride 4 Benzyl alcohol 7 Betablockers 3 Bevacizumab 4 D-Biotin 5 BSA 36, 37, 38 Bovine insulin 2 n-butylbenzene 8 Butyl benzoate 7,, 55 Brilliant blue FCF 24 C Caffeine 6, 53 Candesartan cilexetil 26 Carvedilol 26 Catecholamines 25 Chavidine 24 4-Chloroacetanilide 5 Chlorophenol 9 Chloroquine phosphate 6 Chlorpheniramine 8,, 55, 57, 58 Chlorpheniramine maleate 4 Chlortetracycline 3, 32 α-chymotrypsin 36, 38, 4 α-chymotrypsinogen 39 Cinchonine 27 trans-cinnamic acid 29 Citric acid 5 Citrulline (Cit) 43 Clemastine 59 Clindamycin HCl 3 Clonazepam 23 Conalbumin 38 Coordinating compounds 58 Copper 8-quinolinolate 9 Corticosterone 22 Cortisol 22 Cortisone 22 Cyanidin 35 Cyanidin-3-O-arabinoside 35 Cyanidin-3-O-galactoside 35 Cyanidin-3-O-glucoside 35 Cyanocobalamin 34, 5, 53 Cyclamate Na Cysteine (Cys) 43 Cytidine 52 Cytochrome-C 36, 38 Cytosine 48, 49, 5, 52 D Daidzein 29, 34 Daidzin 34 Delphinidin 35 Delphinidin-3-O-arabinoside 35 Delphinidin-3-O-galactoside 35 Delphinidin-3-O-glucoside 35 Dextromethorphan 8,, 55, 57, 58 Diazepam 23 Dichlorophenols 9 Dihydroquinine 27 3,4-Dihydroxymandelic acid (DOMA) 25 3,4-Dihydroxyphenylacetic acid (DOPAC) 25 3,4-Dihydroxyphenylalanine (DOPA) 25,2-Dimethoxyl benzene 5 Diquat 3 Docosahexaenoic acid 9 Dopamine hydrochloride (DA) 25 Doxycycline 32 Duloxetine hydrochloride 7 E Eicosapentaenoic acid 9 β-endorphin 39, 4 γ-endorphin 4 Epinephrine hydrochloride 25 Erythorbic acid 34, 53 Erythromycin 2, 28 Erythromycin estolate 28 Erythromycin ethylsuccinate 28 α-estradiol 22 β-estradiol 22 Estriol 22 Estrone 22 Etizolam 3 F Fexofenadine hydrochloride 22, 23 Flavonoids 29 Flazasulfuron 9 Folic acid 5 Formic acid 5, 58 Fumaric acid 5 Fumonisin B-3 6 G Genistein 34 Genistin 34 Glutamic acid (Glu) 43 Glutamine (Gln) 43 Glycidic acid 5 Glycine (Gly) 43 Glycitein 34 Glycitin 34 Glycyrrhizin acid ammonium salt 4 Guaiacol 5 Guanine 48, 49, 5 Guanosine 52 H Halosulfuronmethyl 9 Hexobarbital 27 Hinokitiol 55, 58 L-Histidine (His) 42, 43 Human insulin 2, 36 Homovanillic acid (HVA) 25 Hydrochlorothiazide 26 2-Hydroxyacetophenone 5 2-Hydroxyalprazolam 23 Hydrocortison 22 5-Hydroxyindoleacetic acid (5HlAA) 25 Hydroxychloroquine sulfate 6 2-Hydroxyprogesterone 22 5-Hydroxytryptamine hydrochloride (5HT) 25 I Ibuprofen 8 Imipramine 23 Insulin 2, 36, 37, 38, 39 D-Isoascorbic acid 5, 53 Isobutyl p-hydrozybenzoate 3 Isochavicine 24 Isoleucine (Ile) 43 Isopiperine 24 K Kaempferol 29 L Lactic acid 5 Lactoferrin 37 β-lactoglobulin A 36, 38, 39 Leucin (Leu) 43 Leu-Enkephalin 39 Lincomycin HCl 3 Linoleic acid 9 α-linolenic acid 9 γ-linolenic acid 9 Luteolin 29 Lysine (Lys) 43 Lysozyme 36, 38, 39 M Macrolide antibiotics 2 Maleic acid sodium salt 4, 5 L-Malic acid 5 Malonic acid 5 6 -O-Malonyldaidzin 34 62

63 Substance index 6 -O-Malonylgenistin O-Malonylglycitin 34 Malvidin 35 Malvidin-3-O-arabinoside 35 Malvidin-3-O-galactoside 35 Malvidin-3-O-glucoside 35 Mecoprop 9 Met-Enkephalin 4 Metformin hydrochloride 52 L- Methionine (Met) 42, 43 3-Methoxy-4-hydroxyphenylglycol (MHPG) 25 3-Methoxytyramine hydrochloride (3MT) 25 Methyl benzoate 5, 55, 58 4-Methylcatechol 24 Methyl p-hydrozybenzoate 29 2-Methylresorcinol 24 2, 5-Methylresorcinol 24 Metoprolol 3 N Nadolol 3 Naphazolin HCl 4 Naphthalene 8 Neostigmine methylsulfate 4 Neurotensin 4 Nicotinamide 34, 5, 53 Nicotinic acid 3, 34, 5, 53 4-Nitrocatechol 24 4-Nitrophenol 5 Noradrenaline hydrochloride (NA) 25 Norepinephrine hydrochloride 25 Nortriptyline 23 O Oleic acid 9 Oligonucleotides 44, 45 Organic acid 5, 58 Ornithine HCl (Orn) 43 Orotic acid 53 Oxalic acid 5 Oxazepam 23 Oxine-copper 9 Oxytetracycline 3, 32 Oxytocin 39, 4 P Palmitoleic acid 9 D-(+)-Pantothenic acid calcium salt 5 Paracetamol 5 Paraquat 3 Pentobarbital 27 Peonidin 35 Peonidin-3-O-arabinoside 35 Peonidin-3-O-galactoside 35 Peonidin-3-O-glucoside 35 Peptides 4, 42 Pesticides 33 Petunidin 35 Petunidin-3-O-arabinoside 35 Petunidin-3-O-galactoside 35 Petunidin-3-O-glucoside 35 Phenacetine 6 Phenobarbital 27 Phenformin hydrochloride 52 Phenol 7, 5 Phenylalanine (Phe) 43 Pindolol 3 Piperine 24 Porcine insulin 2 Prednisone 22 Progesterone 22 Proline (Pro) 43 Propranolol 3, 56 Propionic acid 5, 58 n-propylbenzene 8 Propyl paraben 8, Propyl p-hydroxybenzoate 55, 58 Pyridoxal HCl 5 Pyridoxine HCl 4, 34, 5, 53 Pyrocatechol 5, 24 Q Quinine 27 8-Quinolinol 8 R Rebaudioside A 4 Resorcinole 24 Riboflavin 34, 5, 53 Ribonuclease A 38 mirna 45 sirna 45 S Saccharin Salicylic acid 6, 29 Scopolamine 27 Secobarbital 27 Serine (Ser) 43 Serotonin hydrochloride 25 Siduron 9 Soy isoflavones 34 Spiramycin 2 Stevioside hydrate 4 Succinic acid 5 Sulpha drugs 2 Sulphamerazine 2 Sulphamethoxazole 2 Sulphathiazole 2 T Tartaric acid 5 Temazepam 23 o-terphenyl 8 Testosterone 8 Tetracycline antibiotics 3, 32 Tetrahydrozoline HCl 4 Thiamine HCl 34, 5, 53 Thiram 9 Threonine (Thr) 43 Thymine 48, 49, 5 Toluol 23 Transferin 36 Triazolam 3 Triclopyr 9 Triphenylene 8 Tryptophan (Trp) 25, 43 Tyrosine (Tyr) 25, 43 U Uracil 2, 48, 49, 5, 52 Uridine 52 V Valine (Val) 43 Valsartan 26 Vanillylmandelic acid (VMA) 25 Verapamil 52 Vitamin B 34, 5, 53 Vitamin B2 34, 5, 53 Vitamin B3 34, 5, 53 Vitamin B5 5 Vitamin B6 34, 5, 53 Vitamin B7 5 Vitamin B2 34, 5, 53 Vitamin C 34, 5, 53 Vitamin D2 2, 2 Vitamin D3 2, 2 W Water-soluble vitamins 34, 5, 53 * Application data by courtesy YMC Co., Ltd. The following brands, trademarks, or service marks are the property of the listed company and/or its subsidiaries. Every effort has been taken to ensure this list is accurate at the time of printing this brochure. Capcell Pak MG-II is a trademark of Shiseido Co., Ltd.w Inertsil ODS-3V, Inertsil ODS-3, Inertsil ODS-4 are trademarks of GL Sciences, inc.. Acquity UPLC BEH, Acquity UPLC CSH C8, Atlantis dc8, Atlantis T3, Bio Resolve RP mab, SunFire C8, Symmetry C8, X-Bridge BEH, X-Bridge Protein BEH, XTerra MS C8 are trademarks of Waters Corp. Aeris, Gemini, Gemini NX, Kinetex are trademarks of Phenomenex, Inc. Hypersil GOLD is a trademark of Thermo Fisher Scientific. Kromasil Eternity is a trademark of EKA Chemicals AB. Advance Bio RP-mAb, Zorbax Extend-C8, Zorbax StableBond, Zorbax Eclipse Plus are trademarks of Agilent Technologies, Inc.. L-column is a trademark of Chemicals Evaluation and Research Institute. Ascentis Express is a trademark of Sigma-Aldrich. 63

64 Ordering information YMC-Triart.9 µm, UHPLC columns (max. pressure, bar) Phase Column ID (mm) Column length (mm) Guard cartridges* with 5 mm length (pack of 3) 2. TA2SP9-22PT TA2SP9-32PT TA2SP9-52PT TA2SP9-L52PT TA2SP9-2PT TA2SP9-52PT TA2SP9-E5QCC** C8 C8 ExRS 2. TA2SP9-2QPT TA2SP9-3QPT TA2SP9-5QPT TA2SP9-L5QPT TA2SP9-QPT TA2SP9-5QPT TA2SP9-E5QCC** 3. TA2SP9-53PT TA2SP9-L53PT TA2SP9-3PT TA2SP9-53PT TA2SP9-E53CC 2. TAR8SP9-22PT TAR8SP9-32PT TAR8SP9-52PT TAR8SP9-L52PT TAR8SP9-2PT TAR8SP9-52PT TAR8SP9-E5QCC** 2. TAR8SP9-2QPT TAR8SP9-3QPT TAR8SP9-5QPT TAR8SP9-L5QPT TAR8SP9-QPT TAR8SP9-5QPT TAR8SP9-E5QCC** 3. TAR8SP9-53PT TAR8SP9-L53PT TAR8SP9-3PT TAR8SP9-53PT TAR8SP9-E53CC 2. TO2SP9-22PT TO2SP9-32PT TO2SP9-52PT TO2SP9-L52PT TO2SP9-2PT TO2SP9-52PT TO2SP9-E5QCC** C8 2. TO2SP9-2QPT TO2SP9-3QPT TO2SP9-5QPT TO2SP9-L5QPT TO2SP9-QPT TO2SP9-5QPT TO2SP9-E5QCC** 3. TO2SP9-53PT TO2SP9-L53PT TO2SP9-3PT TO2SP9-53PT TO2SP9-E53CC 2. TB3SP9-22PT TB3SP9-32PT TB3SP9-52PT TB3SP9-L52PT TB3SP9-2PT TB3SP9-52PT TB3SP9-E5QCC** Bio C4 2. TB3SP9-2QPT TB3SP9-3QPT TB3SP9-5QPT TB3SP9-L5QPT TB3SP9-QPT TB3SP9-5QPT TB3SP9-E5QCC** 3. TB3SP9-53PT TB3SP9-L53PT TB3SP9-3PT TB3SP9-53PT TB3SP9-E53CC 2. TPH2SP9-22PT TPH2SP9-32PT TPH2SP9-52PT TPH2SP9-L52PT TPH2SP9-2PT TPH2SP9-52PT TPH2SP9-E5QCC** Phenyl 2. TPH2SP9-2QPT TPH2SP9-3QPT TPH2SP9-5QPT TPH2SP9-L5QPT TPH2SP9-QPT TPH2SP9-5QPT TPH2SP9-E5QCC** 3. TPH2SP9-53PT TPH2SP9-L53PT TPH2SP9-3PT TPH2SP9-53PT TPH2SP9-E53CC 2. TPF2SP9-22PT TPF2SP9-32PT TPF2SP9-52PT TPF2SP9-L52PT TPF2SP9-2PT TPF2SP9-52PT TPF2SP9-E5QCC** PFP Diol- HILIC 2. TPF2SP9-2QPT TPF2SP9-3QPT TPF2SP9-5QPT TPF2SP9-L5QPT TPF2SP9-QPT TPF2SP9-5QPT TPF2SP9-E5QCC** 3. TPF2SP9-53PT TPF2SP9-L53PT TPF2SP9-3PT TPF2SP9-53PT TPF2SP9-E53CC 2. TDH2SP9-22PT TDH2SP9-32PT TDH2SP9-52PT TDH2SP9-L52PT TDH2SP9-2PT TDH2SP9-52PT TDH2SP9-E5QCC** 2. TDH2SP9-2QPT TDH2SP9-3QPT TDH2SP9-5QPT TDH2SP9-L5QPT TDH2SP9-QPT TDH2SP9-5QPT TDH2SP9-E5QCC** 3. TDH2SP9-53PT TDH2SP9-L53PT TDH2SP9-3PT *Guard cartridge holder required, part no. XPCHUHP **Guard cartridge: 2. mm ID YMC-Triart.9 µm, metal-free UHPLC columns (max. pressure, bar) Phase Column ID (mm) Column length (mm) 5 5 C8 2. TA2SP9-5QPTP TA2SP9-QPTP TA2SP9-5QPTP C8 ExRS 2. TAR8SP9-5QPTP TAR8SP9-QPTP TAR8SP9-5QPTP C8 2. TO2SP9-5QPTP TO2SP9-QPTP TO2SP9-5QPTP Bio C4 2. TB3SP9-5QPTP TB3SP9-QPTP TB3SP9-5QPTP Phenyl 2. TPH2SP9-5QPTP TPH2SP9-QPTP TPH2SP9-5QPTP PFP 2. TPF2SP9-5QPTP TPF2SP9-QPTP TPF2SP9-5QPTP Diol-HILIC 2. TDH2SP9-5QPTP TDH2SP9-QPTP TDH2SP9-5QPTP 64

65 Ordering information YMC-Triart.9 µm, /6 /32 fitting*, microlc capillary columns (max. pressure 6 bar) Phase Column ID (µm) Column length (mm) Guard cartridges** with 5 mm length C8 C8 ExRS C8 Bio C4 Phenyl PFP Diol- HILIC (pack of 3) TA2SP9-5HAU TA2SP9-5JAU TAR8SP9-5HAU TAR8SP9-5JAU TO2SP9-5HAU TO2SP9-5JAU TB3SP9-5HAU TB3SP9-5JAU TPH2SP9-5HAU TPH2SP9-5JAU TPF2SP9-5HAU TPF2SP9-5JAU TDH2SP9-5HAU TDH2SP9-5JAU TA2SP9-L5HAU TA2SP9-L5JAU TAR8SP9-L5HAU TAR8SP9-L5JAU TO2SP9-L5HAU TO2SP9-L5JAU TB3SP9-L5HAU TB3SP9-L5JAU TPH2SP9-L5HAU TPH2SP9-L5JAU TPF2SP9-L5HAU TPF2SP9-L5JAU TDH2SP9-L5HAU TDH2SP9-L5JAU TA2SP9-HAU TA2SP9-JAU TAR8SP9-HAU TAR8SP9-JAU TO2SP9-HAU TO2SP9-JAU TB3SP9-HAU TB3SP9-JAU TPH2SP9-HAU TPH2SP9-JAU TPF2SP9-HAU TPF2SP9-JAU TDH2SP9-HAU TDH2SP9-JAU TA2SP9-5HAU TA2SP9-5JAU TAR8SP9-5HAU TAR8SP9-5JAU TO2SP9-5HAU TO2SP9-5JAU TB3SP9-5HAU TB3SP9-5JAU TPH2SP9-5HAU TPH2SP9-5JAU TPF2SP9-5HAU TPF2SP9-5JAU TDH2SP9-5HAU TDH2SP9-5JAU TA2SP9-E5HAU TA2SP9-E5JAU TAR8SP9-E5HAU TAR8SP9-E5JAU TO2SP9-E5HAU TO2SP9-E5JAU TB3SP9-E5HAU TB3SP9-E5JAU TPH2SP9-E5HAU TPH2SP9-E5JAU TPF2SP9-E5HAU TPF2SP9-E5JAU TDH2SP9-E5HAU TDH2SP9-E5JAU * YMC capillary columns are available with /6 (-32 thread) or with /32 (6-4 thread) connections. The connection size is indicated by the terminal letters of the order code: /6 fittings end with AU; /32 fittings end with RU. For ordering /32 connections, simply exchange AU by RU. ** no holder required, comes with a column coupler YMC-Triart 3 µm, high pressure rated analytical columns (max. pressure 45 bar) Phase Column ID (mm) Column length (mm) Guard cartridges* with mm length (pack of 5) C8 C8 ExRS C8 Bio C4 Phenyl PFP Diol- HILIC 2. TA2S3-2QPTH TA2S3-H3QPTH TA2S3-5QPTH TA2S3-L5QPTH TA2S3-QPTH TA2S3-5QPTH TA2S3-QGC 3. TA2S3-53PTH TA2S3-L53PTH TA2S3-3PTH TA2S3-53PTH TA2S3-3GC 4.6 TA2S3-H346PTH TA2S3-546PTH TA2S3-L546PTH TA2S3-46PTH TA2S3-546PTH TA2S3-2546PTH TA2S3-4GC 2. TAR8S3-2QPTH TAR8S3-H3QPTH TAR8S3-5QPTH TAR8S3-L5QPTH TAR8S3-QPTH TAR8S3-5QPTH TAR8S3-QGC 3. TAR8S3-53PTH TAR8S3-L53PTH TAR8S3-3PTH TAR8S3-53PTH TAR8S3-3GC 4.6 TAR8S3-H346PTH TAR8S3-546PTH TAR8S3-L546PTH TAR8S3-46PTH TAR8S3-546PTH TAR8S3-2546PTH TAR8S3-4GC 2. TO2S3-2QPTH TO2S3-H3QPTH TO2S3-5QPTH TO2S3-L5QPTH TO2S3-QPTH TO2S3-5QPTH TO2S3-QGC 3. TO2S3-53PTH TO2S3-L53PTH TO2S3-3PTH TO2S3-53PTH TO2S3-3GC 4.6 TO2S3-H346PTH TO2S3-546PTH TO2S3-L546PTH TO2S3-46PTH TO2S3-546PTH TO2S3-2546PTH TO2S3-4GC 2. TB3S3-2QPTH TB3S3-H3QPTH TB3S3-5QPTH TB3S3-L5QPTH TB3S3-QPTH TB3S3-5QPTH TB3S3-QGC 3. TB3S3-53PTH TB3S3-L53PTH TB3S3-3PTH TB3S3-53PTH TB3S3-3GC 4.6 TB3S3-H346PTH TB3S3-546PTH TB3S3-L546PTH TB3S3-46PTH TB3S3-546PTH TB3S3-2546PTH TB3S3-4GC 2. TPH2S3-2QPTH TPH2S3-H3QPTH TPH2S3-5QPTH TPH2S3-L5QPTH TPH2S3-QPTH TPH2S3-5QPTH TPH2S3-QGC 3. TPH2S3-53PTH TPH2S3-L53PTH TPH2S3-3PTH TPH2S3-53PTH TPH2S3-3GC 4.6 TPH2S3-H346PTH TPH2S3-546PTH TPH2S3-L546PTH TPH2S3-46PTH TPH2S3-546PTH TPH2S3-2546PTH TPH2S3-4GC 2. TPF2S3-2QPTH TPF2S3-H3QPTH TPF2S3-5QPTH TPF2S3-L5QPTH TPF2S3-QPTH TPF2S3-5QPTH TPF2S3-QGC 3. TPF2S3-53PTH TPF2S3-L53PTH TPF2S3-3PTH TPF2S3-53PTH TPF2S3-3GC 4.6 TPF2S3-H346PTH TPF2S3-546PTH TPF2S3-L546PTH TPF2S3-46PTH TPF2S3-546PTH TPF2S3-2546PTH TPF2S3-4GC 2. TDH2S3-2QPTH TDH2S3-H3QPTH TDH2S3-5QPTH TDH2S3-L5QPTH TDH2S3-QPTH TDH2S3-5QPTH TDH2S3-QGC 3. TDH2S3-53PTH TDH2S3-L53PTH TDH2S3-3PTH TDH2S3-53PTH TDH2S3-3GC 4.6 TDH2S3-H346PTH TDH2S3-546PTH TDH2S3-L546PTH TDH2S3-46PTH TDH2S3-546PTH TDH2S3-2546PTH TDH2S3-4GC *Guard cartridge holder required, part no. XPGCH-Q 65

66 Ordering information YMC-Triart 3 µm, metal-free analytical columns (max. pressure 45 bar) Phase Column ID (mm) Column length (mm) 5 5 C TA2S3-5QPTP TA2S3-546PTP TA2S3-QPTP TA2S3-46PTP TA2S3-5QPTP TA2S3-546PTP C8 ExRS TAR8S3-5QPTP TAR8S3-546PTP TAR8S3-QPTP TAR8S3-46PTP TAR8S3-5QPTP TAR8S3-546PTP C TO2S3-5QPTP TO2S3-546PTP TO2S3-QPTP TO2S3-46PTP TO2S3-5QPTP TO2S3-546PTP Bio C TB3S3-5QPTP TB3S3-546PTP TB3S3-QPTP TB3S3-46PTP TB3S3-5QPTP TB3S3-546PTP Phenyl TPH2S3-5QPTP TPH2S3-546PTP TPH2S3-QPTP TPH2S3-46PTP TPH2S3-5QPTP TPH2S3-546PTP PFP TPF2S3-5QPTP TPF2S3-546PTP TPF2S3-QPTP TPF2S3-46PTP TPF2S3-5QPTP TPF2S3-546PTP Diol- HILIC TDH2S3-5QPTP TDH2S3-546PTP TDH2S3-QPTP TDH2S3-46PTP TDH2S3-5QPTP TDH2S3-546PTP YMC-Triart 3 µm, analytical columns (max. pressure 2/25 bar) Phase Column ID (mm) Column length (mm) Guard cartridges* with mm length (pack of 5) C8 C8 Phenyl PFP Diol- HILIC 2. TA2S3-22WT TA2S3-32WT TA2S3-52WT TA2S3-L52WT TA2S3-2WT TA2S3-52WT TA2S3-QGC 3. TA2S3-53WT TA2S3-L53WT TA2S3-3WT TA2S3-53WT TA2S3-3GC 4.6 TA2S3-546WT TA2S3-L546WT TA2S3-46WT TA2S3-546WT TA2S3-2546WT TA2S3-4GC 2. TO2S3-22WT TO2S3-32WT TO2S3-52WT TO2S3-L52WT TO2S3-2WT TO2S3-52WT TO2S3-QGC 3. TO2S3-53WT TO2S3-L53WT TO2S3-3WT TO2S3-53WT TO2S3-3GC 4.6 TO2S3-546WT TO2S3-L546WT TO2S3-46WT TO2S3-546WT TO2S3-2546WT TO2S3-4GC 2. TPH2S3-22WT TPH2S3-32WT TPH2S3-52WT TPH2S3-L52WT TPH2S3-2WT TPH2S3-52WT TPH2S3-QGC 3. TPH2S3-53WT TPH2S3-L53WT TPH2S3-3WT TPH2S3-53WT TPH2S3-3GC 4.6 TPH2S3-546WT TPH2S3-L546WT TPH2S3-46WT TPH2S3-546WT TPH2S3-2546WT TPH2S3-4GC 2. TPF2S3-22WT TPF2S3-32WT TPF2S3-52WT TPF2S3-L52WT TPF2S3-2WT TPF2S3-52WT TPF2S3-QGC 3. TPF2S3-53WT TPF2S3-L53WT TPF2S3-3WT TPF2S3-53WT TPF2S3-3GC 4.6 TPF2S3-546WT TPF2S3-L546WT TPF2S3-46WT TPF2S3-546WT TPF2S3-2546WT TPF2S3-4GC 2. TDH2S3-22WT TDH2S3-32WT TDH2S3-52WT TDH2S3-L52WT TDH2S3-2WT TDH2S3-52WT TDH2S3-QGC 3. TDH2S3-53WT TDH2S3-L53WT TDH2S3-3WT TDH2S3-53WT TDH2S3-3GC 4.6 TDH2S3-546WT TDH2S3-L546WT TDH2S3-46WT TDH2S3-546WT TDH2S3-2546WT TDH2S3-4GC *Guard cartridge holder required, part no. XPGCH-Q 66

67 Ordering information YMC-Triart 3 µm, /6 /32 fitting*, microlc capillary columns (max. pressure 55 bar) Phase Column ID (µm) Column length (mm) Guard columns** with 5 mm length (pack of 3) C8 C8ExRS C8 Bio C4 Phenyl PFP Diol- HILIC 75 TA2S3-5E8AU TA2S3-L5E8AU TA2S3-E8AU TA2S3-5E8AU TA2S3-5FAU TA2S3-L5FAU TA2S3-FAU TA2S3-5FAU 3 TA2S3-5HAU TA2S3-L5HAU TA2S3-HAU TA2S3-5HAU TA2S3-E5HAU 5 TA2S3-5JAU TA2S3-L5JAU TA2S3-JAU TA2S3-5JAU TA2S3-E5JAU 75 TAR8S3-5E8AU TAR8S3-L5E8AU TAR8S3-E8AU TAR8S3-5E8AU TAR8S3-5FAU TAR8S3-L5FAU TAR8S3-FAU TAR8S3-5FAU 3 TAR8S3-5HAU TAR8S3-L5HAU TAR8S3-HAU TAR8S3-5HAU TAR8S3-E5HAU 5 TAR8S3-5JAU TAR8S3-L5JAU TAR8S3-JAU TAR8S3-5JAU TAR8S3-E5JAU 75 TO2S3-5E8AU TO2S3-L5E8AU TO2S3-E8AU TO2S3-5E8AU TO2S3-5FAU TO2S3-L5FAU TO2S3-FAU TO2S3-5FAU 3 TO2S3-5HAU TO2S3-L5HAU TO2S3-HAU TO2S3-5HAU TO2S3-E5HAU 5 TO2S3-5JAU TO2S3-L5JAU TO2S3-JAU TO2S3-5JAU TO2S3-E5JAU 75 TB3S3-5E8AU TB3S3-L5E8AU TB3S3-E8AU TB3S3-5E8AU TB3S3-5FAU TB3S3-L5FAU TB3S3-FAU TB3S3-5FAU 3 TB3S3-5HAU TB3S3-L5HAU TB3S3-HAU TB3S3-5HAU TB3S3-E5HAU 5 TB3S3-5JAU TB3S3-L5JAU TB3S3-JAU TB3S3-5JAU TB3S3-E5JAU 75 TPH2S3-5E8AU TPH2S3-L5E8AU TPH2S3-E8AU TPH2S3-5E8AU TPH2S3-5FAU TPH2S3-L5FAU TPH2S3-FAU TPH2S3-5FAU 3 TPH2S3-5HAU TPH2S3-L5HAU TPH2S3-HAU TPH2S3-5HAU TPH2S3-E5HAU 5 TPH2S3-5JAU TPH2S3-L5JAU TPH2S3-JAU TPH2S3-5JAU TPH2S3-E5JAU 75 TPF2S3-5E8AU TPF2S3-L5E8AU TPF2S3-E8AU TPF2S3-5E8AU TPF2S3-5FAU TPF2S3-L5FAU TPF2S3-FAU TPF2S3-5FAU 3 TPF2S3-5HAU TPF2S3-L5HAU TPF2S3-HAU TPF2S3-5HAU TPF2S3-E5HAU 5 TPF2S3-5JAU TPF2S3-L5JAU TPF2S3-JAU TPF2S3-5JAU TPF2S3-E5JAU 75 TDH2S3-5E8AU TDH2S3-L5E8AU TDH2S3-E8AU TDH2S3-5E8AU TDH2S3-5FAU TDH2S3-L5FAU TDH2S3-FAU TDH2S3-5FAU 3 TDH2S3-5HAU TDH2S3-L5HAU TDH2S3-HAU TDH2S3-5HAU TDH2S3-E5HAU 5 TDH2S3-5JAU TDH2S3-L5JAU TDH2S3-JAU TDH2S3-5JAU TDH2S3-E5JAU * YMC capillary columns are available with /6 (-32 thread) or with /32 (6-4 thread) connections. The connection size is indicated by the terminal letters of the order code: /6 fittings end with AU; /32 fittings end with RU. For ordering /32 connections, simply exchange AU by RU. ** no holder required, comes with a column coupler YMC-Triart.9 and 3 µm Method Development Kits Phases Dimensions Particle size Part No. C8 / C8 ExRS / Phenyl C8 / C8 / Phenyl C8 / PFP / Diol-HILIC 5 x 2. mm.9 µm TATARTPHSP9-5QPT 3 µm TATARTPHS3-5QPTH.9 µm TATOTPHSP9-5QPT 3 µm TATOTPHS3-5QPTH.9 µm TATPFTDHSP9-5QPT 3 µm TATPFTDHS3-5QPTH 67

68 Ordering information YMC-Triart 5 µm, high pressure rated analytical columns (max. pressure 45 bar) Phase Column ID (mm) Column length (mm) Guard cartridges* with mm length (pack of 5) C8 C8 ExRS C8 Bio C4 Phenyl PFP Diol- HILIC 2. TA2S5-2QPTH TA2S5-H3QPTH TA2S5-5QPTH TA2S5-L5QPTH TA2S5-QPTH TA2S5-5QPTH TA2S5-QGC 3. TA2S5-53PTH TA2S5-L53PTH TA2S5-3PTH TA2S5-53PTH TA2S5-3GC 4.6 TA2S5-H346PTH TA2S5-546PTH TA2S5-L546PTH TA2S5-46PTH TA2S5-546PTH TA2S5-2546PTH TA2S5-4GC 2. TAR8S5-2QPTH TAR8S5-H3QPTH TAR8S5-5QPTH TAR8S5-L5QPTH TAR8S5-QPTH TAR8S5-5QPTH TAR8S5-QGC 3. TAR8S5-53PTH TAR8S5-L53PTH TAR8S5-3PTH TAR8S5-53PTH TAR8S5-3GC 4.6 TAR8S5-H346PTH TAR8S5-546PTH TAR8S5-L546PTH TAR8S5-46PTH TAR8S5-546PTH TAR8S5-2546PTH TAR8S5-4GC 2. TO2S5-2QPTH TO2S5-H3QPTH TO2S5-5QPTH TO2S5-L5QPTH TO2S5-QPTH TO2S5-5QPTH TO2S5-QGC 3. TO2S5-53PTH TO2S5-L53PTH TO2S5-3PTH TO2S5-53PTH TO2S5-3GC 4.6 TO2S5-H346PTH TO2S5-546PTH TO2S5-L546PTH TO2S5-46PTH TO2S5-546PTH TO2S5-2546PTH TO2S5-4GC 2. TB3S5-2QPTH TB3S5-H3QPTH TB3S5-5QPTH TB3S5-L5QPTH TB3S5-QPTH TB3S5-5QPTH TB3S5-QGC 3. TB3S5-53PTH TB3S5-L53PTH TB3S5-3PTH TB3S5-53PTH TB3S5-3GC 4.6 TB3S5-H346PTH TB3S5-546PTH TB3S5-L546PTH TB3S5-46PTH TB3S5-546PTH TB3S5-2546PTH TB3S5-4GC 2. TPH2S5-2QPTH TPH2S5-H3QPTH TPH2S5-5QPTH TPH2S5-L5QPTH TPH2S5-QPTH TPH2S5-5QPTH TPH2S5-QGC 3. TPH2S5-53PTH TPH2S5-L53PTH TPH2S5-3PTH TPH2S5-53PTH TPH2S5-3GC 4.6 TPH2S5-H346PTH TPH2S5-546PTH TPH2S5-L546PTH TPH2S5-46PTH TPH2S5-546PTH TPH2S5-2546PTH TPH2S5-4GC 2. TPF2S5-2QPTH TPF2S5-H3QPTH TPF2S5-5QPTH TPF2S5-L5QPTH TPF2S5-QPTH TPF2S5-5QPTH TPF2S5-QGC 3. TPF2S5-53PTH TPF2S5-L53PTH TPF2S5-3PTH TPF2S5-53PTH TPF2S5-3GC 4.6 TPF2S5-H346PTH TPF2S5-546PTH TPF2S5-L546PTH TPF2S5-46PTH TPF2S5-546PTH TPF2S5-2546PTH TPF2S5-4GC 2. TDH2S5-2QPTH TDH2S5-H3QPTH TDH2S5-5QPTH TDH2S5-L5QPTH TDH2S5-QPTH TDH2S5-5QPTH TDH2S5-QGC 3. TDH2S5-53PTH TDH2S5-L53PTH TDH2S5-3PTH TDH2S5-53PTH TDH2S5-3GC 4.6 TDH2S5-H346PTH TDH2S5-546PTH TDH2S5-L546PTH TDH2S5-46PTH TDH2S5-546PTH TDH2S5-2546PTH TDH2S5-4GC YMC-Triart 5 µm, metal-free analytical columns (max. pressure 45 bar) Phase Column ID (mm) Column length (mm) 5 5 C TA2S5-5QPTP TA2S5-546PTP TA2S5-QPTP TA2S5-46PTP TA2S5-5QPTP TA2S5-546PTP C8 ExRS TAR8S5-5QPTP TAR8S5-546PTP TAR8S5-QPTP TAR8S5-46PTP TAR8S5-5QPTP TAR8S5-546PTP C TO2S5-5QPTP TO2S5-546PTP TO2S5-QPTP TO2S5-46PTP TO2S5-5QPTP TO2S5-546PTP Bio C TB3S5-5QPTP TB3S5-546PTP TB3S5-QPTP TB3S5-46PTP TB3S5-5QPTP TB3S5-546PTP Phenyl TPH2S5-5QPTP TPH2S5-546PTP TPH2S5-QPTP TPH2S5-46PTP TPH2S5-5QPTP TPH2S5-546PTP PFP TPF2S5-5QPTP TPF2S5-546PTP TPF2S5-QPTP TPF2S5-46PTP TPF2S5-5QPTP TPF2S5-546PTP Diol- HILIC TDH2S5-5QPTP TDH2S5-546PTP TDH2S5-QPTP TDH2S5-46PTP TDH2S5-5QPTP TDH2S5-546PTP 68

69 Ordering information YMC-Triart 5 µm analytical columns (max. pressure 2/25 bar) Phase Column ID (mm) Column length (mm) Guard cartridges* with mm length (pack of 5) C8 C8 2. TA2S5-22WT TA2S5-32WT TA2S5-52WT TA2S5-L52WT TA2S5-2WT TA2S5-52WT TA2S5-QGC 3. TA2S5-53WT TA2S5-L53WT TA2S5-3WT TA2S5-53WT TA2S5-3GC 4.6 TA2S5-546WT TA2S5-L546WT TA2S5-46WT TA2S5-546WT TA2S5-2546WT TA2S5-4GC ** TA2S5-5WT TA2S5-25WT TA2S5-CC 2. TO2S5-22WT TO2S5-32WT TO2S5-52WT TO2S5-L52WT TO2S5-2WT TO2S5-52WT TO2S5-QGC 3. TO2S5-53WT TO2S5-L53WT TO2S5-3WT TO2S5-53WT TO2S5-3GC 4.6 TO2S5-546WT TO2S5-L546WT TO2S5-46WT TO2S5-546WT TO2S5-2546WT TO2S5-4GC ** TO2S5-5WT TO2S5-25WT TO2S5-CC 2. TPH2S5-22WT TPH2S5-32WT TPH2S5-52WT TPH2S5-L52WT TPH2S5-2WT TPH2S5-52WT TPH2S5-QGC Phenyl 3. TPH2S5-53WT TPH2S5-L53WT TPH2S5-3WT TPH2S5-53WT TPH2S5-3GC 4.6 TPH2S5-546WT TPH2S5-L546WT TPH2S5-46WT TPH2S5-546WT TPH2S5-2546WT TPH2S5-4GC ** TPH2S5-5WT TPH2S5-25WT TPH2S5-CC 2. TPF2S5-22WT TPF2S5-32WT TPF2S5-52WT TPF2S5-L52WT TPF2S5-2WT TPF2S5-52WT TPF2S5-QGC PFP Diol- HILIC 3. TPF2S5-53WT TPF2S5-L53WT TPF2S5-3WT TPF2S5-53WT TPF2S5-3GC 4.6 TPF2S5-546WT TPF2S5-L546WT TPF2S5-46WT TPF2S5-546WT TPF2S5-2546WT TPF2S5-4GC ** TPF2S5-5WT TPF2S5-25WT TPF2S5-CC 2. TDH2S5-22WT TDH2S5-32WT TDH2S5-52WT TDH2S5-L52WT TDH2S5-2WT TDH2S5-52WT TDH2S5-QGC 3. TDH2S5-53WT TDH2S5-L53WT TDH2S5-3WT TDH2S5-53WT TDH2S5-3GC 4.6 TDH2S5-546WT TDH2S5-L546WT TDH2S5-46WT TDH2S5-546WT TDH2S5-2546WT TDH2S5-4GC *Guard cartridge holder required, part no. XPGCH-Q (2., 3, 4 mm ID) XPCHSPW ( mm ID) **Max. pressure bar 69

70 Ordering information YMC-Triart 5 µm, /6 /32 fitting*, microlc capillary columns (max. pressure 55 bar) Phase Column ID (µm) Column length (mm) Guard columns** with 5 mm length (pack of 3) 75 TA2S5-5E8AU TA2S5-L5E8AU TA2S5-E8AU TA2S5-5E8AU C8 TA2S5-5FAU TA2S5-L5FAU TA2S5-FAU TA2S5-5FAU 3 TA2S5-5HAU TA2S5-L5HAU TA2S5-HAU TA2S5-5HAU TA2S5-E5HAU 5 TA2S5-5JAU TA2S5-L5JAU TA2S5-JAU TA2S5-5JAU TA2S5-E5JAU 75 TAR8S5-5E8AU TAR8S5-L5E8AU TAR8S5-E8AU TAR8S5-5E8AU C8 ExRS TAR8S5-5FAU TAR8S5-L5FAU TAR8S5-FAU TAR8S5-5FAU 3 TAR8S5-5HAU TAR8S5-L5HAU TAR8S5-HAU TAR8S5-5HAU TAR8S5-E5HAU 5 TAR8S5-5JAU TAR8S5-L5JAU TAR8S5-JAU TAR8S5-5JAU TAR8S5-E5JAU 75 TO2S5-5E8AU TO2S5-L5E8AU TO2S5-E8AU TO2S5-5E8AU C8 TO2S5-5FAU TO2S5-L5FAU TO2S5-FAU TO2S5-5FAU 3 TO2S5-5HAU TO2S5-L5HAU TO2S5-HAU TO2S5-5HAU TO2S5-E5HAU 5 TO2S5-5JAU TO2S5-L5JAU TO2S5-JAU TO2S5-5JAU TO2S5-E5JAU 75 TB3S5-5E8AU TB3S5-L5E8AU TB3S5-E8AU TB3S5-5E8AU Bio C4 TB3S5-5FAU TB3S5-L5FAU TB3S5-FAU TB3S5-5FAU 3 TB3S5-5HAU TB3S5-L5HAU TB3S5-HAU TB3S5-5HAU TB3S5-E5HAU 5 TB3S5-5JAU TB3S5-L5JAU TB3S5-JAU TB3S5-5JAU TB3S5-E5JAU 75 TPH2S5-5E8AU TPH2S5-L5E8AU TPH2S5-E8AU TPH2S5-5E8AU Phenyl TPH2S5-5FAU TPH2S5-L5FAU TPH2S5-FAU TPH2S5-5FAU 3 TPH2S5-5HAU TPH2S5-L5HAU TPH2S5-HAU TPH2S5-5HAU TPH2S5-E5HAU 5 TPH2S5-5JAU TPH2S5-L5JAU TPH2S5-JAU TPH2S5-5JAU TPH2S5-E5JAU 75 TPF2S5-5E8AU TPF2S5-L5E8AU TPF2S5-E8AU TPF2S5-5E8AU PFP TPF2S5-5FAU TPF2S5-L5FAU TPF2S5-FAU TPF2S5-5FAU 3 TPF2S5-5HAU TPF2S5-L5HAU TPF2S5-HAU TPF2S5-5HAU TPF2S5-E5HAU 5 TPF2S5-5JAU TPF2S5-L5JAU TPF2S5-JAU TPF2S5-5JAU TPF2S5-E5JAU 75 TDH2S5-5E8AU TDH2S5-L5E8AU TDH2S5-E8AU TDH2S5-5E8AU Diol- HILIC TDH2S5-5FAU TDH2S5-L5FAU TDH2S5-FAU TDH2S5-5FAU 3 TDH2S5-5HAU TDH2S5-L5HAU TDH2S5-HAU TDH2S5-5HAU TDH2S5-E5HAU 5 TDH2S5-5JAU TDH2S5-L5JAU TDH2S5-JAU TDH2S5-5JAU TDH2S5-E5JAU * YMC capillary columns are available with /6 (-32 thread) or with /32 (6-4 thread) connections. The connection size is indicated by the terminal letters of the order code: /6 fittings end with AU; /32 fittings end with RU. For ordering /32 connections, simply exchange AU by RU. ** no holder required, comes with a column coupler 7

71 Ordering information YMC-Triart 5 µm in YMC-Actus high-throughput semipreparative hardware (max. pressure 3 bar) Phase Column ID (mm) Column length (mm) Guard cartridges* with mm length C8 C8 ExRS C8 Bio C4 Phenyl PFP (pack of 2) 2 TA2S5-52WX TA2S5-L52WX TA2S5-2WX TA2S5-52WX TA2S5-252WX TA2S5-2CC 3 TA2S5-53WX TA2S5-L53WX TA2S5-3WX TA2S5-53WX TA2S5-253WX TA2S5-3CC 5 TA2S5-553DX TA2S5-53DX TA2S5-553DX TA2S5-2553DX TA2S5-553DXG** 2 TAR8S5-52WX TAR8S5-L52WX TAR8S5-2WX TAR8S5-52WX TAR8S5-252WX TAR8S5-2CC 3 TAR8S5-53WX TAR8S5-L53WX TAR8S5-3WX TAR8S5-53WX TAR8S5-253WX TAR8S5-3CC 5 TAR8S5-553DX TAR8S5-53DX TAR8S5-553DX TAR8S5-2553DX TAR8S5-553DXG** 2 TO2S5-52WX TO2S5-L52WX TO2S5-2WX TO2S5-52WX TO2S5-52WX TO2S5-2CC 3 TO2S5-53WX TO2S5-L53WX TO2S5-3WX TO2S5-53WX TO2S5-53WX TO2S5-3CC 5 TO2S5-553DX TO2S5-53DX TO2S5-553DX TO2S5-553DX TO2S5-553DXG** 2 TB3S5-52WX TB3S5-L52WX TB3S5-2WX TB3S5-52WX TB3S5-52WX TB3S5-2CC 3 TB3S5-53WX TB3S5-L53WX TB3S5-3WX TB3S5-53WX TB3S5-53WX TB3S5-3CC 5 TB3S5-553DX TB3S5-53DX TB3S5-553DX TB3S5-553DX TB3S5-553DXG** 2 TPH2S5-52WX TPH2S5-L52WX TPH2S5-2WX TPH2S5-52WX TPH2S5-52WX TPH2S5-2CC 3 TPH2S5-53WX TPH2S5-L53WX TPH2S5-3WX TPH2S5-53WX TPH2S5-53WX TPH2S5-3CC 5 TPH2S5-553DX TPH2S5-53DX TPH2S5-553DX TPH2S5-553DX TPH2S5-553DXG** 2 TPF2S5-52WX TPF2S5-L52WX TPF2S5-2WX TPF2S5-52WX TPF2S5-252WX TPF2S5-2CC 3 TPF2S5-53WX TPF2S5-L53WX *guard TPF2S5-3WX cartridge holder required, TPF2S5-53WX part no. XPCHSPW2 TPF2S5-253WX (2 mm ID)/XPCHSPW3 TPF2S5-3CC (3 mm) 5 TPF2S5-553DX TPF2S5-53DX **no holder TPF2S5-553DX required for 5 TPF2S5-2553DX x 5 mm ID guard columns TPF2S5-553DXG** (no cartridge) YMC-Triart, preparative bulk media *Guard cartridge holder required, part no. XPCHSPW2 (2 mm ID)/XPCHSPW3 (3 mm ID) ** no holder required for 5 x 5 mm ID guard columns (no cartridge) YMC-Triart C8-S YMC-Triart C8-S Pore size (nm) Particle size (µm) Product Code Pore size (nm) Particle size (µm) Product Code TAS2S TOS2S 2 5 TAS2S6 2 5 TOS2S6 2 TAS2S2 2 TOS2S2 Please inquire for the corresponding brochures: NOTE: customised particle sizes and pore sizes are available on request. Contact YMC Europe GmbH for further details. YMC-Triart Prep Brochure YMC Biochromatography Columns - Brochure MicroLC Brochure 7

72 Your local distributor: Achrom N.V. Phlippostraat Machelen-Zulte BELGIUM Telephone: (+32) 9/ Fax: (+32) 9/ achrom@achrom.be Europe GmbH Schöttmannshof 9 D Dinslaken Germany Phone +49()264/427-, Fax +49()264/ Schweiz GmbH Im Wasenboden Basel Switzerland Phone , Fax CO., LTD. YMC Karasuma-Gojo Bld. 284 Daigo-cho, Karasuma Nishiiru Gojo-dori Shimogyo-ku, Kyoto 6-86 Japan Phone +8() , Fax +8() YMC-Triart_9/28_EN_V2.