Epithelium-1. Hanan Jafar BDS.MSc.PhD
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1 Epithelium-1 Hanan Jafar BDS.MSc.PhD
2 General features Epithelium is an avascular tissue composed of cells that cover the exterior body surfaces and line internal closed cavities and tubes. It also forms the secretory portion of glands and their ducts. Specialized epithelial cells function as receptors for the special senses. Can be derived from all embryonic germ layers. Epithelial cells are polarized and resting on basement membrane.
3 Epithelium lining skin, mouth, nose and anus: ectoderm Epithelium lining respiratory system, digestive tract and glands of digestive tract: endoderm Epithelium lining blood vessels: mesoderm
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9 Cell polarity The epithelial cell shows two domains 1- Apical 2- Baso-lateral Each domain shows modifications to suit its functions
10 Functions Protection Transcellular transport Secretion Absorption (selective permeability) Involved in sensation
11 Classifications & Naming of Epithelia According to number of layers Simple one layer of cells Stratified more than one layer of cells
12 Classification & Naming of Epithelia According to shape of cells Squamous cells wider than tall (plate or scale like) Cuboidal cells are as wide as tall, as in cubes Columnar cells are taller than they are wide, like columns
13 Naming Epithelia Naming the epithelia includes both the layers (first) and the shape of the cells (second) i.e. stratified cuboidal epithelium The name may also include any accessory structures Keratin Goblet cells Cilia Special epithelial tissues (don t follow naming convention) Pseudostratified Transitional
14 Squamous Simple Cuboidal Epithelium Pseudostratified Stratified Columnar Squamous Cuboidal Transitional keratinized nonkeratinized Columnar
15 Simple epithelium
16 Stratified epithelium
17 Simple Epithelia Simple Squamous Locations: Vascular system (endothelium) Body cavities (mesothelium) Bowman s capsule (kidney) Major function: Exchange
18 Endothelium is the simple squamous epithelium that lines the lumen of the cardiovascular system
19 Mesothelium is the simple squamous epithelium that lines serous cavities (peritoneal, pleural, and pericardial cavities) and coats many of the organs in these cavities
20 Stratified Epithelia Contain two or more layers of cells Regenerate from below Major role is protection Are named according to the shape of cells at apical layer Mitotic activity in stratified epithelium is limited to the basal layer (under normal conditions).
21 Stratified Squamous Epithelium Specific types Keratinized contain the protective protein keratin Surface cells are dead and full of keratin Non-keratinized forms moist lining of body openings Function Protects underlying tissues in areas subject to abrasion Location Keratinized forms epidermis Non-keratinized forms lining of mouth, esophagus, and vagina
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23 Goblet cells produce mucus Cilia (larger than microvilli) sweep mucus Ciliated Pseudostratified Columnar Epithelium with goblet cells
24 Transitional Epithelium (Urothelium) Description Basal cells usually cuboidal or columnar Superficial cells dome-shaped or squamous Function stretches and permits distension of urinary bladder Location Lines ureters, urinary bladder and part of urethra
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27 Apical domain It is the part of the cell that faces the lumen or external environment (the free surface of the cell). The apical modifications are: Microvilli Cilia Stereocilia It is rich in ion channels, carrier proteins and hydrolytic enzymes.
28 Microvilli - Present mainly in absorptive cells. - Their number and size vary according to the degree of activity of the cell. - They are usually crowded on the cell apex forming the striated border in the intestine and the brush border in the kidney.
29 Structure - The microvillus is 1µ in length, 0.1µ in width. - Contains a core of actin filaments. - Actin filaments are cross-linked with villin. - The actin filaments are inserted into the terminal web. - The terminal web is a network of actin and spectrin supported by myosin and IF at the apical part of the cell. - The microvillus is covered by glycocalyx.
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31 Terminal web is a cytoskeletal feature at the apical pole in many epithelial cells
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33 Microvilli under LM and EM
34 Cilia - Motile cytoplasmic hair like projections capable of moving fluid and particles along epithelial surfaces. - Measurements: length 5-10μ, diameter 0.2 μ. Number of cilia/cell is variable and ranges cilium/cell. - They move rhythmically and rapidly in one direction
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37 - The core of the cilium is called axoneme. - The axoneme consists of longitudinal microtubules arranged as 9 (doublets) peripheral surrounding 2 (singlets) central (9+2). - The doublets are composed of 2 subunits A & B. - Subunit A is formed of 13 protofilaments. - Subunit B is formed of 10 protofilaments.
38 - Neighboring doublets are connected by nexin. - Doublets are connected to the singlets by radial spokes. - Dynein radiates form subunit A to subunit B. - Dynein has ATPase activity. - Cilia are attached to basal bodies similar in structure to centrioles.
39 -Cilia wiggle to move mucus - Found in respiratory epithelium
40 Stereocilia -Stereocilia are long immotile microvilli present in the epididymis and inner ear. They have special functions in these places -They are longer than typical microvilli, more widely separated and branched - They contain actin filaments
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