PARASITOLOGICAL EXAMINATIONS CATALOGUE OF SERVICES AND PRICE LIST
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1 INSTITUTE OF PARASITOLOGY Biomedical Research Center Seltersberg Justus Liebig University Giessen Schubertstrasse Giessen Germany Office: +49 (0) Fax: +49 (0) Coprological & hematological diagnostic lab: +49 (0) Serological & molecular biological diagnostic lab: +49 (0) S CATALOGUE OF SERVICES AND PRICE LIST Valid as from 1 st January 2017 (prices 1 plus VAT 19%) Dogs, cats, wild canids and felids, mustelids, raccoons, bears etc. Please note: Medical samples must be BREAK- AND LEAF-PROOFED PACKED in accordance with the postal regulations. 1 Prices according to the governmental fee schedules in Germany ( Gebührenordnung für Tierärzte, GOT ), version of 30 th June 2008, and the fee the Justus Liebig University Giessen ( Satzung über die Entgeltregelung für die Kliniken und Institute des Fachbereichs Veterinärmedizin ), version of 18 th October Institute of Parasitology JLU Giessen Catalogue of Services & Price List DOG CAT (version 1 March 2018) Page 1
2 OBLIGATORY S for the IMPORTATION of DOGS to AUSTRALIA in fee B-03 B-12 Leishmania (serology) ELISA (plates; detection of L. infantum antibodies in serum/) Ehrlichia (serology) IFAT (detection of Ehrlichia antibodies in serum/) aa ab Z-01 Certificate (examination report for B-03 + B-12) ac to NEW ZEALAND in fee B-11 Babesia spp. (PCR) PCR (detection of Babesia specific DNA in whole blood) ba B-10 B-06 Babesia gibsoni (serology) IFAT (detection of B. gibsoni antibodies in serum/) Dirofilaria immitis (serology) Antigen ELISA (detection of D. immitis antigen in serum/) bb bc Z-01 Certificate (examination report for B-01 + B-05 + B-06 + B-10 + B-12) bd Institute of Parasitology JLU Giessen Catalogue of Services & Price List DOG CAT (version 1 March 2018) Page 2
3 OBLIGATORY S for the IMPORTATION of DOGS to the REPUBLIC OF SOUTH AFRICA in fee B-01 Babesia gibsoni and Trypanosoma evansi Giemsa-stained blood smears (detection of haemoparasites) Blood smears or 2 4 ml whole blood ca B-05 Dirofilaria immitis microfilariae Microfilarial filtration test (detection of microfilariae in whole blood) 2 4 ml whole blood cb B-06 Dirofilaria immitis (serology) Antigen ELISA (detection of D. immitis antigen in serum/) cc B-03 B-10 Leishmania (serology) ELISA (plates; detection of L. infantum antibodies in serum/) Babesia gibsoni (serology) IFAT (detection of B. gibsoni antibodies in serum/) cd ce B-16 Trypanosoma (serology) CATT (card agglutination test for the detection of T. evansi antibodies in serum/) cf Z-01 Certificate (examination report for B-01 + B-05 + B-06 + B-03 + B-10 + B-16) cg Institute of Parasitology JLU Giessen Catalogue of Services & Price List DOG CAT (version 1 March 2018) Page 3
4 FAECAL SAMPLES in fee K-01 K-03 K-04 K-06 K-08 K-09 Gastrointestinal parasites, liver flukes (helminths, coccidia) Combined sedimentation-flotation method (detection of helminth eggs and coccid oocysts in faeces; no Cryptosporidium) Gastrointestinal parasites, liver flukes (helminths, coccidia), incl. amoeba and flagellates Sodium Acetate Formaldehyde (SAF) method (detection of helminth eggs, coccid oocysts, stages of amoeba and flagellates in faeces; no Cryptosporidium) Lungworms Baermann funnel method (detection and differentiation of lungworm larvae in faeces) Giardia Coproantigen ELISA (plates; detection of Giardia antigen in faeces) Cryptosporidium Coproantigen ELISA (plates; detection of Cryptosporidium antigen in faeces) Tritrichomonas foetus Copro PCR (detection of T. foetus DNA in faeces) 5 10 g faeces (chestnut size) 5 10 g faeces (chestnut size) 5 10 g faeces (chestnut size) 2 3 g faeces (native or preserved in SAF solution) 2 3 g faeces (native or preserved in SAF solution) Alternatively to K-01 in case of small amount of faeces a c d f h 2 3 g faeces i Institute of Parasitology JLU Giessen Catalogue of Services & Price List DOG CAT (version 1 March 2018) Page 4
5 FAECAL SAMPLES in fee K-10 Taeniids Adhesive tape method (detection of taeniid eggs at the perianal fur) Eggs of Taenia spp. and Echinococcus spp. cannot be differentiated morphologically! Adhesive strips (e.g., Scotch ) from perianal fur j K-11 Echinococcus multilocularis or E. granulosus Copro PCR (detection of specific DNA in faeces) 5 10 g faeces (chestnut size) k K-13 Specimens of parasite-like objects Macroscopical + microscopical examination (parasite-like objects from faeces or organs) Native specimens or preserved in 70% ethanol *for 5 specimens 15.00* 311.1m Combination of several faecal examination methods in fee K-p1 Gastrointestinal parasites, incl. Giardia Combined sedimentation-flotation method + Giardia coproantigen ELISA 5 10 g faeces (chestnut size) K-01 + K a Institute of Parasitology JLU Giessen Catalogue of Services & Price List DOG CAT (version 1 March 2018) Page 5
6 Combination of several faecal examination methods in fee K-p2 Gastrointestinal parasites, incl. Giardia + lungworms Combined sedimentation-flotation method + Giardia coproantigen ELISA + Baermann funnel method g faeces K-01 + K-04 + K b K-p3 Gastrointestinal parasites, incl. Giardia + Tritrichomonas Combined sedimentation-flotation method + Giardia coproantigen ELISA + Tritrichomonas copro PCR 10 g faeces K-01 + K-06 + K c BLOOD SAMPLES (WHOLE BLOOD, SERUM, PLASMA), TISSUE SAMPLES in fee B-01 Haemoparasites Giemsa-stained blood smears (detection of haemoparasites) Blood smears or 2 4 ml whole blood a B-02 Leishmania (microscopical examination) Giemsa-stained biopsy sample / HE-stained tissue specimen (detection of Leishmania stages in tissue) Biopsy material or tissue specimen b B-03 Leishmania (serology) ELISA (detection of L. infantum antibodies in serum/) c Institute of Parasitology JLU Giessen Catalogue of Services & Price List DOG CAT (version 1 March 2018) Page 6
7 BLOOD SAMPLES (WHOLE BLOOD, SERUM, PLASMA), TISSUE SAMPLES in fee B-04 Leishmania (PCR) PCR (detection of Leishmania DNA in tissue), conjunctival swab sample or biopsy material d B-05 Microfilaria Microfilarial filtration test (detection of microfilariae in whole blood) 2 4 ml whole blood e B-06 Dirofilaria immitis (serology) Antigen ELISA (detection of D. immitis antigen in serum/) f B-07 (Micro)filariae (PCR) PCR (detection of specific DNA in whole blood and molecular biological differentiation of the species) Worm specimens or g B-08 Angiostrongylus vasorum (serology) Antigen ELISA (detection of A. vasorum antigen in serum/) h B-09 Babesia (serology) IFAT (detection of Babesia antibodies in serum/) i B-10 Babesia gibsoni (serology) IFAT (detection of B. gibsoni antibodies in serum/) j Institute of Parasitology JLU Giessen Catalogue of Services & Price List DOG CAT (version 1 March 2018) Page 7
8 BLOOD SAMPLES (WHOLE BLOOD, SERUM, PLASMA), TISSUE SAMPLES in fee B-11a B-11b B-12 B-13 B-14 B-15a B-15b B-16 Babesia spp. (PCR) PCR (detection of Babesia specific DNA in whole blood without species differentiation) Babesia spp. (PCR) PCR + sequencing (detection of Babesia specific DNA in whole blood, followed by sequencing for species differentiation) Ehrlichia (serology) IFAT (detection of Ehrlichia antibodies in serum/) Ana (serology) ELISA (detection of Ana antibodies in serum/) Ana phagocytophilum (PCR) PCR (detection of A. phagocytophilum DNA in whole blood) Ehrlichia/Ana (PCR) PCR (detection of Ehrlichia-/Ana specific DNA in whole blood, without species differentiation) Ehrlichia/Ana (PCR) PCR + sequencing (detection of Ehrlichia-/Ana specific DNA in whole blood, followed by sequencing for species differentiation) Trypanosoma (serology) CATT (card agglutination test for the detection of T. evansi antibodies in serum/) ka lb m n o pa pb q Institute of Parasitology JLU Giessen Catalogue of Services & Price List DOG CAT (version 1 March 2018) Page 8
9 BLOOD SAMPLES (WHOLE BLOOD, SERUM, PLASMA), TISSUE SAMPLES in fee B-17 B-18 B-19 Trypanosoma (PCR) PCR (detection of Trypanosoma DNA in whole blood or liquor) Toxo gondii (serology) IHA (detection of T. gondii antibodies in serum/) Toxo gondii (PCR) PCR (detection of T. gondii DNA in tissue) or liquor r s Tissue sample t B-20a Neospora (serology) IFAT (detection of Neospora antibodies in serum/) ua B-21 B-24 B-25 B-26 Neospora (PCR) PCR (detection of N. caninum DNA in tissue) Echinococcosis PCR (detection of DNA of E. multilocularis or E. granulosus sensu stricto in tissue or cyst fluid) Lungworms in tissue PCR (detection of specific DNA in tissue, molecular biological differentiation of the lungworm species) Parasites in histological specimens Histopathology (morphological differentiation of parasites in HE-stained histological specimens) Tissue sample (e.g., brain, musculature) Tissue sample, cyst fluid v y Helminth specimens or lung tissue sample za Histological specimens zb Institute of Parasitology JLU Giessen Catalogue of Services & Price List DOG CAT (version 1 March 2018) Page 9
10 BLOOD SAMPLES (WHOLE BLOOD, SERUM, PLASMA), TISSUE SAMPLES in fee B-27 Parasite specimens, isolated or in tissue sample Universal PCR + sequencing (detection of specific DNA, molecular biological differentiation of species or genus) Native specimens or preserved in 70% ethanol, tissue specimens on demand zc Kombination of several blood examination methods in fee B-p1 Travel profile chronic 1 Ehrlichia IFAT + Babesia IFAT + Leishmania ELISA stay in an endemic region long time ago B-03 + B-09 + B a B-p2 Travel profile chronic 2 Ehrlichia IFAT + Babesia IFAT + Leishmania ELISA + Dirofilaria immitis antigen ELISA stay in an endemic region long time ago B03 + B-06 + B-09 + B b B-p3 Travel profile acute Giemsa-stained blood smears + Babesia PCR in case of acute symptoms B-01 + B c Institute of Parasitology JLU Giessen Catalogue of Services & Price List DOG CAT (version 1 March 2018) Page 10
11 SKIN SCAPINGS, FUR SAMPLES, ARTHOPODS from the ENVIRONMENT in fee H-01 H-02 H-03 H-04 Ectoparasites (mange mites) Macroscopical + microscopical examination + KOH digestion (detection of ectoparasite stages in skin or fur samples) Fur mites Adhesive tape method (detection of Cheyletiella mites etc.) Arthropod specimens from animals Macroscopical + microscopical examination (e.g., lice, fleas, ticks) Arthropods from other origin Macroscopical + microscopical examination Skin or ear scrapings a Adhesive tapes (e.g. Scotch ) from fur Native specimens or preserved in 70% ethanol Native specimens or preserved in 70% ethanol *for 5 specimens b c d URINE SAMPLES in fee U-01 Capillaria plica Sedimentation method (detection of C. plica eggs in urine) 5 ml urine a Morphological or molecular biological examination of other samples or parasites not mentioned above on demand! Institute of Parasitology JLU Giessen Catalogue of Services & Price List DOG CAT (version 1 March 2018) Page 11
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