CURRICULUM VITAE KAUSTUV SAHA

Transcription

1 CURRICULUM VITAE KAUSTUV SAHA University of Florida, McKnight Brain Institute, Department of Neuroscience 1149 Newell Drive; Room L4-151; Gainesville; Florida 32611; USA Tel: ACADEMIC CREDENTIALS 2011 PhD (Pharmacology), Department of Pharmacological and Pharmaceutical Sciences, College of Pharmacy, University of Houston. Project: Changes in GRK3 and Norepinephrine Responsiveness in Locus Coeruleus Neurons are Associated with Learned Helplessness after Repeated Forced Swim Stress Masters in Pharmacy (Pharmacology), Faculty of Pharmacy, Hamdard University, India. Project: influence of carvedilol on the anti-epileptic action of Lamotrigine in mice Bachelor in Pharmacy, Delhi Institute of Pharmaceutical Science and Research, University of Delhi, India. POSITION AND EXPERIENCE Positions: present Postdoctoral Associate, Department of Neuroscience, University of Florida, Gainesville, Florida Teaching Assisstant, Dept. of Pharmaceutical and Pharmacological Sciences, University of Houston, Houston, Texas Junior Research Fellow, Faculty of Pharmacy, Hamdard University, New Delhi, India. Mentoring Experience: present Mentoring undergraduate students Paran Davari, Laura Villarroel, Soha Alam, Anna Shao, Glenn Garron, Jessica Marz and Jonas Backer at University of Florida in the lab of Dr. Habibeh Khoshbouei Mentored Undergraduate student Kayla Bicol at University of Houston in the lab of Dr. Douglas Eikenburg PUBLICATIONS Research articles: 1. Saha K, Sambo D, Richardson BD, Lin LM, Butler B, Villarroel L, Khoshbouei H. Intracellular Methamphetamine Prevents the Dopamine-induced Enhancement of Neuronal Firing. J Biol Chem Jun Saha K, Swant J, Khoshbouei H. Single cell measurement of dopamine release with simultaneous voltage-clamp and amperometry. Journal of Visualised Experiments

2 3. Butler B, Saha K, Khoshbouei H. Alpha-Synuclein regulation of dopamine transporter. Translational Neuroscience, 2012, 3(3), Taneja M, Salim S, Saha K, Happe HK, Qutna N, Petty F, Bylund D B, and Eikenburg DC. Differential effects of inescapable stress on locus coeruleus GRK3, alpha2- adrenoceptor and CRF1 receptor levels in learned helpless and non-helpless rats: a potential link to stress resilience. Behavioural brain research, 2011, 221, Salim S, Asghar M, Taneja M, Hovatta I, Wu YL, Saha K, Sarraj N, and Hite B. Novel role of RGS2 in regulation of antioxidant homeostasis in neuronal cells. FEBS letters, 2011, 585, Salim S, Asghar M, Chugh G, Taneja M, Xia Z., and Saha K. Oxidative stress: a potential recipe for anxiety, hypertension and insulin resistance. Brain research, , Salim S, Sarraj N, Taneja M, Saha K, Tejada-Simon MV., and Chugh G. Moderate treadmill exercise prevents oxidative stress-induced anxiety-like behavior in rats. Behavioural brain research, 2010, 208, Velpandian T, Saha K, Ravi AK, Kumari SS, Biswas NR, and Ghose S. Ocular hazards of the colors used during the festival-of-colors (Holi) in India--malachite green toxicity. Journal of hazardous materials, 2007, 139, Accepted for review 1. Saha K, Goodwin SJ, Patel T, Davari P, Villarroel L, Sambo D, Alonge T, Lin M, Butler B, Steger R, Khoshbouei H. Psychostimulant Occupancy of Dopamine Transporters Affects Its Lateral Mobility and Membrane Microdomain Distribution 2. Richardson BD, Saha K, Swant J, Cabrera E, Sambo D, Zou MF, Newman AH, Khoshbouei H. Membrane potential shapes regulation of dopamine transporter trafficking at the plasma membrane Posters and Abstracts: 1. Saha K, Butler B, Lin M, Coleman J, Giasson B, Golde T, Khoshbouei H. Alpha-synuclein Elevation Shapes Dopamine Neurotransmission via a DAT-dependent mechanism. Society for Neuroscience. Oct Saha K, Sambo D, Richardson BD, Lin LM, Butler B, Villarroel L, Davari P, Olson S, Marz J, Shao A, Garron G, Alam S, Khoshbouei H. Extracellular dopamine-induced enhancement of neuronal firing is prevented by Intracellular methamphetamine. CARE symposium University of Florida. 3. Saha K, Lin M, Sambo D, Garron G, Alam S, Shao A, Alonge T, Ahmari N, Buch S, Khoshbouei H. HIV-tat 101 protein regulation of electrophysiological properties of midbrain dopaminergic neurons. 2nd Scientific Symposium, 2015, Society of Personalised Nano- Medicine.

3 4. Saha K, Lin M, Sambo D, Garron G, Alam S, Shao A, Alonge T, Ahmari N, Buch S, Khoshbouei H. HIV-tat 101 protein regulation of electrophysiological properties of midbrain dopaminergic neurons. University of Florida, College of Medicine, Celebration of Research Poster Session Saha K, Angoli D, Villarroel L, Davari P, Khoshbouei H. Intracellular Methamphetamine decreases dopamine-dependent inward current via dopamine transporter. University of Florida, College of Medicine, Celebration of Research Poster Session Saha K, Angoli D, Villarroel L, Davari P, Khoshbouei H. Intracellular Methamphetamine decreases dopamine-dependent inward current via dopamine transporter. CARE symposium, University of Florida, April Saha K, Angoli D, Villarroel L, Davari P, Khoshbouei H. Intracellular S(+)- Methamphetamine decreases dopamine-dependent inward current via dopamine transporter. Brain Awareness Week Conference, North Central Florida Chapter, March 10, Saha K, Richardson B, Lin M, Anjoli D, Davari P, Villarroel L, Khoshbouei H. Intracellular methamphetamine prevents the dopamine-induced enhancement of neuronal firing. Brain, biology and behavior: Translational Research in Addiction, March 15 16, 2014, San Antonio, Texas. Poster # Saha K, Angoli D, Villarroel L, Davari P, Khoshbouei H. Intracellular S(+)- Methamphetamine decreases dopamine-dependent inward current via dopamine transporter. Society for Neuroscience; Nov 2013, Control # 2013-S-9022-SfN. 10. Saha K, Villarroel L, Davari P, Mehr SE, Zarindast MR, Richardson B, Khoshbouei H. Amphetamine and methamphetamine differentially regulate biophysical properties of dopamine transporter. Experimental Biology Meeting. April 2013.Abst# Davari P, Villarroel V, Saha K, Cartier E, Khoshbouei H. Methamphetamine and Amphetamine influence biophysical properties of Dopamine Transporter in a Phosphorylation dependent manner. Experimental Biology Meeting. April 2013.Abst# Saha K, Villarroel L, Davari P, Mehr SE, Zarindast MR, Richardson B, Khoshbouei H. Postdoc Research Symposium University of Florida, Gainesville. Amphetamine and methamphetamine differentially regulate biophysical properties of dopamine transporter. Postdoc Research Symposium University of Florida, Gainesville. 13. Saha K, Goodwin S, Swant J, Butler, Khoshbouei H. Unlike amphetamine-exposed transporter the methamphetamine exposed transporter is fully responsive to consecutive drug application. University of Florida, College of Medicine, Celebration of Research Poster Session Saha K, Swant J, Butler B, Lin M, Azghadi SH, Davari P, Goodwin S, Khoshbouei H; Society for Neuroscience; Oct 2012; The membrane localization of DAT molecule determines its movement kinetics.

4 15. Saha K, Goodwin S, Swant J, Butler, Khoshbouei H. Unlike amphetamine-exposed transporter the methamphetamine exposed transporter is fully responsive to consecutive drug application. NIDA mini-symposium at Society for Neuroscience; Oct Saha, K., Eikenburg, D.C., Taneja, M., Salim, S., and Bicol, K.: Repeated Forced Swim Stress Induces Learned Helplessness in Rats. Experimental Biology Abst. # Taneja, M., Saha, K., Patki, J., Lau, Y.S., and Eikenburg, D.C.: Sustained separation of rats into stress-susceptible and stress-resilient groups by repeated exposure to inescapable unpredictable stress. Experimental Biology Saha, K., Taneja, M., Salim, S., Ziburkus, J., and Eikenburg, D.C.: Repeated Forced swim Stress-induced behavioral changes are associated with changes in GRK3 in rat Locus Coeruleus. Soc. Neuroscience, Abst. # Taneja, M., Saha, K., and Eikenburg, D.C.: Reduced GRK3 levels in Locus Coeruleus after unpredictable inescapable stress is associated with increased stress susceptibility. Soc. Neuroscience, Abst. # Salim, S., Taneja, M., Chugh, G., Saha, K, Sarraj, N., Vollert, C., Tejada-Simon, MV. Eikenburg, D.C, Hovatta, I. A potential protective role of RGS2 in oxidative-stress mediated anxious behavior in rats Experimental Biology, 2009 Abst. #LB359. Oral presentation: 1. Saha K, Richardson B, Lin M, Anjoli D, Davari P, Villarroel L, Khoshbouei H. Intracellular methamphetamine prevents the dopamine-induced enhancement of neuronal firing. Postdoc Research Symposium, University of Florida, April 21, Saha K, Khoshbouei H. Intracellular methamphetamine prevents the dopamine-induced enhancement of neuronal firing. Data Blitz competition. Brain Awareness Week Conference, North Central Florida Chapter, March 10, 2014, 3. Saha K, Villarroel L, Davari P, Mehr SE, Zarindast MR, Richardson B, Khoshbouei H. Amphetamine and methamphetamine differentially regulate biophysical properties of dopamine transporter. Experimental Biology Meeting. April FELLOWSHIPS AND GRANTS McKnight Brain Institute Fellowship award, McKnight Brain Institute, University of Florida Makhtoob Alam Endowed Scholarship from University of Houston for academic performance Graduate Teaching Assistantship, University of Houston Junior Research Fellowship, Ministry of Human Resource and Development, Govt. of Delhi, India HONORS AND AWARDS: 2014 Travel Award, Brain, biology and behavior: Translational Research in Addiction, March 15 16, 2014, San Antonio, Texas. Poster # 57

5 2013 Central Florida Chapter of Society for Neuroscience Travel Award, Society for Neuroscience, San Diego Travel Award, National Institute of Drug Abuse mini-symposium, New Orleans. PROFESSIONAL SOCIETY MEMBERSHIPS: present Society for Neuroscience present North Central Florida chatper of Society for Neuroscience present The American Society for Pharmacology and Experimental Therapeutics present Indian Pharmacological Society present Registered Pharmacist, Pharmacy Council of India, India. EXPERTISE AND SKILLS: Electrophysiology: Whole-cell patch clamp, extracellular, voltage and current clamp recordings. Amperometry: Carbon fiber amperometry Total Internal Reflection Simultaneous patch-clamp TIRF, Live-cell TIRF. Fluorescent microscopy (TIRF) Confocal microscopy: Live-cell imaging, FRET, FRAP, FLIP Animal behavioral assessments: Rodent models to evaluate effect of acute and chronic stress, depression, anxiety, memory, motor-co-ordination, epilepsy, endurance treadmill and exercise training. Ex-Vivo Pharmacology: Tissue experiments involving rat aortic rings, guinea pig and rat ileum, isolated heart preparation, and rat uterus. Immunology: SDS PAGE, Immunohistochemistry and Immunoprecipitation Pharmacological approach: Whole animal drug administration via intraperitoneal and subcutaneous routes. Local infusion of drugs in brain by implantation of osmotic pumps. Cell-culture work, ipscs Software Packages: SPSS, GraphPad prizm. Analytical: HPLC, spectrophotomete Formulations: Drug formulations in the form of tablets, emulsions, liposome