Gag. Gag-Gag. TSG101 Nedd4. Two-Hybrid HIV Gag. Two-Hybrid. Two-Hybrid
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1 CytoTrap HIV Gag AnnexinA2 AUP1 AUP1 HIV AUP1 N Gag CytoTrap Gag-Gag TSG101 Nedd4 Two-Hybrid Two-Hybrid HIVGag Gag Two-Hybrid
2 HIV Gag Gag HIV Two-Hybrid CytoTrap cdna Gag Gag CytoTrap Gag CytoTrap Gag-Gag Gag-Gag HIV CytoTrap Gag CytoTrap Stratagene cdna pmyr HIV-1 Gag psos Saccharomyces cerevisiae cdc25ha MATa ura3-52 his3-200 ade2-101 lys2-801 trp1-901 leu cdc25-2 Gal + 1 CytoTrap Gag-Gag HIV-1 Gag pmyr psos Gag/pMyr Gag/pSOS cdc25ha Gag-Gag cdc25ha OD 600 =0.1 Enamine OD 630 DNA transfection cdna AUP1 isoform1 isoform 2 PCR pcdna3-flag-hisc HIV-1 cdna pnl432 Lipofectamine 2000Invitrogen HeLa, 293T cotransfection Gag-GFP pnl432 derivative RT-PCR : HeLa, 293T T : Jurkat, CEM174, SupT1 : U937 RNA oligo dt+random primers PCR cdna Clontech SDS-PAGE Western blotting HIV-1 p24ca FLAG 3.7% FLAG TGN, PDI, CD63 HIV HIV-1 p24 capture ELISA KitZeptometrix CytoTrap Gag pmyr 800, cgmp Annexin A2AUP1 cgmp RAS Gag Annexin A2 Gag HIV AUP1 AUP1 mrna AUP1 mrna isoform 1unspliced form isoform 2spliced formisoform 1 N
3 AUP1 mrna isoform 2 isoform isoform 2 isoform AUP1 HIV-1 AUP1 isoform 2 AUP1 C N isoform 2 CD63 TGN46 N PDI HIV-1 Gag isoform 2 C Gag N Gag AUP1 HIV-1 AUP1 HIV-1 cdna HeLa isoform 2 isoform 1 isoform 1 N C N HIV C HIV AUP1 N 40 CytoTrap Gag-Gag CytoTrap Gag-Gag 96 high-throughput format 20,000 IC 50 Gag-Gag S. Kawada, T. Goto, H. Haraguchi, A. Ono, & Y. Morikawa. Dominant negative inhibition of human immunodeficiency virus particle production by the non-myristoylated form of Gag. J. Virol. 82: (2008) E. Urano, S. Shimizu, Y. Futahashi, M. Hamatake, Y. Morikawa, N. Takahashi, H. Fukazawa, N. Yamamoto, & J. Komano.
4 Cyclin K/CPR4 inhibits primate lentiviral replication by inactivating Tat/P-TEFb-dependent LTR transcription. AIDS 22: (2008) S. Yamayoshi, T. Noda, H. Ebihara, H. Goto, Y. Morikawa, I. S. Lukashevich, G. Neumann, H. Feldmann, & Y. Kawaoka. Ebola virus matrix VP40 protein uses the COPII transport system for its intracellular transport. Cell Host Microbe 3: (2008) A. Ryo, N. Tsurutani, K. Ohba, R. Kimura, J. Komano, M. Nishi, H. Soeda, S. Hattori, K. Perrem, M. Yamamoto, J. Chiba, J. Mimaya, K. Yoshimura, S. Matsushita, M. Honda, A. Yoshimura, T. Sawasaki, I. Aoki, Y. Morikawa, & N. Yamamoto. SOCS1 is an inducible host factor during HIV-1 infection and regulates the intracellular trafficking and stability of HIV-1 Gag. Proc. Natl. Acad. Sci. USA 105: (2008) E. Urano, T. Aoki, Y. Futahashi, T. Murakami, Y. Morikawa, N. Yamamoto, & J. Komano. Substitution of the myristoylation signal of human immunodeficiency virus type 1 Pr55 Gag with the phopholipase C delta 1 pleckstrin homology domain results in infectious pseudovirion production. J. Gen. Virol. (in press) E. Urano, Y. Kariya, Y. Futahashi, R. Ichikawa, M. Hamatake, H. Fukazawa, Y. Morikawa, T. Yoshida, Y. Koyanagi, N. Yamamoto, & J. Komano. Identification of the P-TEFb complex-interacting domain of Brd4 as an inhibitor of HIV-1 replication by functional cdna library screening in MT-4 cells. FEBS Let 582: (2008) Y. Morikawa, T. Goto, D. Yasuoka, F. Momose, & T. Matano. Defect of Human Immunodeficiency Virus Type 2 Gag Assembly in Saccharomyces cerevisiae. J. Virol. 81: (2007) HIV 52: (2007) F. Momose, Y. Kikuchi, K. Komase, & Y. Morikawa. Visualization of microtubulemediated transport of influenza viral progeny ribonucleoprotein. Microbes Infect. 9: (2007) T. Naito, F. Momose, A.Kawaguchi, & K. Nagata. Involvement of Hsp90 in assembly and nuclear import of influenza virus RNA polymerase subunits. J. Virol. 81: (2007) S. Sakuragi, J. Sakuragi, Y. Morikawa, & T. Shioda. Development of a rapid and convenient method for the quantitation of HIV-1 budding. Microbes Infect. 8: (2006) 56: 9-16 (2006) E. Urano, Y. Kariya, Y. Futahashi, M. Hamatake, Y. Morikawa, T. Yoshida, Y. Koyanagi, N. Yamamoto, & J. Komano. Identification of the carboxy-terminal domain of chromatin-associated transcriptional activator bromodomain containing 4 as a specific silencer of HIV-1 replication. CSH Retrovirus Meeting NY DNA J/ HSP40 Co-chaperonine family HIV HIV-1 Gag-Pol RNP HIV RNP AUP1 HIV RNP 31 / E. Urano, S. Shimizu, M. Hamatake, Y. Morikawa, & J. Komano. Upregulating expression of cyclin K/CPR4 limits the replication of HIV-1. CSH Retrovirus Meeting NY HIV 55
5 Gag HIV-1 Gag-Pol NP RNP HRS Gag 30 / Gag-Pol 30/ vrnp 30 / N. Tsurutani, T. Goto, I. S. Ohno, & Y. Morikawa. Identification of host cellular machinery for HIV-1 Gag trafficking. CSH Retrovirus Meeting2006 NY N. Tsurutani, T. Goto, I. S. Ohno, & Y. Morikawa. Identification of host cellular machinery for HIV-1 Gag trafficking. ASCB Meeting2006 AL 21 F. Momose, A. Kawaguchi, A. Iwamatsu, Y. Morikawa & K. Nagata. Stimulation of influenza virus RNA synthesis by a host factor RAF-2 that is bound to viral nucleoprotein and the genome RNA. 13ICNSV HIV Gag HIV Gag RAF-2p36 RNP Y. Morikawa. Dominant negative inhibition of HIV particle production by the nonmyristoylated form of Gag
Received 26 August 2008; revised 10 October 2008; accepted 21 October Available online 7 November Edited by Ivan Sadowski
Identification of the P-TEFb complex-interacting domain of Brd4 as an inhibitor of HIV-1 replication by functional cdna library screening in MT-4 cells Emiko Urano a,b, Yumi Kariya a, Yuko Futahashi a,
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