PATHOLOGY SPECIMEN TUBES QUICK REFERENCE GUIDE : Edition Expires July 2013

Transcription

1 PATHOLOGY SPECIMEN TUBES QUICK REFERENCE GUIDE : Edition Expires July 2013 FOR THE MOST UP TO DATE INFORMATION AND FOR TESTS THAT ARE NOT LISTED HERE PLEASE CHECK THE INTRANET SITE AT: or Group 1 : The following tests can be performed on a single Red Top 4mL SST tube ACE AFP (alpha Foetoprotein) Alcohol alpha 1 antitrypsin Amikacin B12/folate ß2-Microglobulin C3, C4 CA-125 C-reactive protein (CRP) CK Caeruloplasmin Calcium group Carbamazepine CEA Cholesterol Digoxin Ferritin Fructosamine Gentamicin Glucose ( sample must be rec'd < 4 hours post collection.) Haptoglobin HCG HDL IPG Iron (Iron Studies) LDH LFT Lipase Lithium Methotrexate Osmolality Paracetamol Phenobarb Procalcitonin Phenytoin PSA Rheumatoid Factor Salicylate Theophylline Tobramycin Transferrin Triglyceride Troponin U&E Urate Valproate Vancomycin SST 4mL (Red top with gel) Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: July 2012 Page 1 of 12

2 Each of the following GROUPS of tests requires its own 5 ml White Top tube with gel, irrespective of other test and sample requirements. Group 2 TSH, TFT, Insulin, C-peptide, Prolactin, FSH, Estradiol, Vitamin D. Group 3 Serology Viral PCR eg HCv, HBV Group 4 ANA ENA ANCA Copper EPG Selenium Zinc Plain 5mL (White Top with gel) Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: July 2012 Page 2 of 12

3 APTT D-Dimers Fibrinogen INR Coags NB: Fill to thick black arrow Sodium Citrate 3.5 ml (Blue Top) FBC Retics Hb Separate Tube Required for these tests Haemoglobin Alc (HbA1c) Homocysteine (on ice) BNP (must arrive within 4hrs) Cyclosporin Tacrolimus Lead Cadmium PCR EDTA 4mL (Pink Top) Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: July 2012 Page 3 of 12

4 Blood Group and Hold Crossmatch Adults EDTA - Transfusion - 9mL (Purple Top) OR Paediatrics EDTA - Transfusion - 2mL (Purple Top) Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: July 2012 Page 4 of 12

5 Lactate Glucose NB. Glucose can be measured on a Red Top SST tube provided it is received by the lab within 4 hours of collection NB: Always fill the GREY TOP tube to the thick black line Sodium Fluoride/EDTA 2mL (Grey Top) ESR NB: Fill to line, if problems with filling then also send a filled 4ml Pink Top Do not stick label onto inner clear tube Black Rubber Top Tube with Additive Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: July 2012 Page 5 of 12

6 Flow Cytometry Studies (Yellow Top and Pink Top) Separate Tube Required for HIV Loads = 9ml Purple Top (dedicated sample) for this test 9 ml Yellow Top ACD Tube Aluminium Chromium Cobalt 7 ml Navy Top Tube Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: July 2012 Page 6 of 12

7 Ammonia Send on ice Advise Lab. Before collection, Ext ml or 4 ml Green Top Tube Chromosome studies/karyotype FISH for 22q Adult-9mL Child-4mL 9 ml or 4 ml Green Top Tube Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: July 2012 Page 7 of 12

8 Fragile X DNA test Prader Willi Syndrome / Angelman Syndrome Molecular Karyotyping / Microarray Sample must be received in laboratory no later than 2pm Thursday. Enquiries: Ext 8297 PCR : Separate Tube required EDTA 4mL (Pink Top) Swab MC & S only See database for all other swabs Swab (Blue Cap) Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: July 2012 Page 8 of 12

9 Paediatric Tubes Haematology and Transfusion EDTA 2mL (Purple Top) OR EDTA 0.5mL (Pink Top) ( Neonates ) Fill to the line Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: July 2012 Page 9 of 12

10 Paediatric Tubes - Coagulation Sodium Citrate 1mL (Blue) Sodium Citrate 2mL (Blue Top) - Coags Fill to thick black line Bullet with gel 1mL - Biochemistry ( Neonates ) Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: July 2012 Page 10 of 12

11 SPECIMEN LABELLING All sample tubes must show the following details : Patient's surname and given name(s) Date of Birth Unit Record Number Date and time of collection Ward Clear labelling of tubes is essential and handwritten labels are preferred. If patient sticky labels are used then they must be the small ones and placed longitudinally so that the contents and fill level are still visible for the laboratory staff to check. in block capitals to avoid misidentification of the Requestor. RHH Doctors must also use their stamp with their Provider Number on it. Specimens for blood group or crossmatch must be hand labelled and signed by the person who collects the specimen the person who witnesses the specimen collection Blood group or crossmatch samples with pre-printed labels will not be processed. Complete all fields of the dedicated Transfusion Request Form. SPECIMEN TRANSPORT AND DELIVERY All samples must be transported in a sealed Biohazard bag with the sample in the sealable section and the Request Form in the separate pocket. Blood gas or ionised calcium samples are transported in the syringe in which the blood was taken. The needle must be removed before transport and replaced with a protective cap. If transported in the vacuum tube system the samples must be packed with ice and double bagged. All specimens must be accompanied by a completed Request Form signed by the Doctor and with the Doctor's name written Vacuum Tube System Samples may be sent in the appropriate canisters via the vacuum Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: July 2012 Page 11 of 12

12 tube system which has stations situated throughout the hospital. The numbers for Pathology are or All specimens must be in Biohazard bags. Blood cultures should be wrapped in bubble wrap and bagged and only one set of blood culture tubes should be put in one canister. Certain samples such as those for Platelet Function Analysis must not be sent by Vacuum Tube check the Pathology Specimen Database if you are uncertain about sending a specimen via the Vacuum Tube System. Personal Delivery to Pathology. Samples should be brought to Pathology Reception which is located on the 1st floor of the D block (Gaha Wing) in the area near the lifts adjacent to the Clinical School. Occasionally the RHH Intranet is unavailable but the Pathology CIS Website is still operational. In those situations use the direct URLs to connect : RESULTS ONLINE - Direct URL = TESTS AVAILABLE DATABASE - Direct URL = PATHOLOGY WEB PAGE - Direct URL = Hard copy reports are produced throughout the day and distributed to the Wards via the internal mail system. PATHOLOGY RESULTS All reports other than HIV reports are available electronically via the RHH Intranet Digital Medical Record. Internet Explorer is available on PCs in the clinical areas and should be used to access this information; it should be unnecessary for you to phone the labs for Routine Results or for Routine Specimen Container Information Document ID: QS-Proc-35 Author: Dr T. Hartley Authorised by Dr. T Hartley Issued Date: July 2012 Page 12 of 12