Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK 5 Biochemistry Department Homerton University Hospital NHS Foundation Trust Homerton Row London E9 6SR Contact: Paula Wilks Tel: +44 (0) p.wilks@nhs.net Website: Testing performed at the above address only BIOLOGICAL FLUIDS otherwise stated DETAIL OF ACCREDITATION General Chemistry / Toxicology/ Therapeutic drug monitoring Abbott Architect ci8200 LP-BIO-P400 - Abbott Architect ci8200 Operational and Routine Maintenance procedures. LP-BIO-P460_Abbott Architect assay information chart in accordance with manufacturer s instructions., urine Albumin for serum sample, Immunoturbidimetric detection for urine sample Alanine aminotransferase (ALT) Alkaline Phosphatase (ALP) Alpha-1-antitrypsin (A1AT) Amikacin Plasma (lithium heparin) Ammonia Angiotensin Converting Enzyme (ACE) using third party assay from Buhlmann kinetic, urine Amylase Aspartate aminotransferase (AST) Bicarbonate Bile Acids Bilirubin Direct Bilirubin Total β-2 Microglobulin (β2m) Caeruloplasmin, urine Calcium Carbamazepine Cholesterol (HDL) Cholesterol (Total), urine Creatinine Enzymatic Creatinine Kinase (CK) C-Reactive Protein C3 Complement Assessment Manager: FN Page 1 of 10

2 BIOLOGICAL FLUIDS otherwise stated General Chemistry/ Toxicology/ Therapeutic drug monitoring Abbott Architect ci8200 LP-BIO-P400_Abbott Architect ci8200 Operational and Routine Maintenance procedures. LP-BIO-P460_Abbott Architect assay information chart in accordance with manufacturer s instructions. CSF Plasma (fluoride oxalate), urine serum C4 Complement CSF Protein Digoxin Ethanol Fructosamine Gentamicin Gamma-Glutamyl aminotransferase Turbidmetric detection (GGT) Plasma (fluoride oxalate) Glucose CSF(fluoride oxalate) Glucose Haptoglobin Immunoglobulin A Immunoglobulin G Immunoglobulin M Iron Lactate Dehydrogenase (LDH) Plasma (fluoride oxalate) Lactate CSF (fluoride oxalate) Lactate Lithium, urine Magnesium Paracetamol Phenobarbitone Phenytoin Enzyme Immunoassay detection, urine Phosphate Prealbumin (Transthyretin) PSA (Prostate Specific Antigen) Sandwich CMIA Rheumatoid Factor (RF) Salicylate, urine Sodium/ Potassium/ Chloride Indirect ISE (Ion Selective Electrode) Theophylline Enzyme Immunoassay detection Total Protein Transferrin Triglycerides (total) Urine Urate Assessment Manager: FN Page 2 of 10

3 BIOLOGICAL FLUIDS otherwise stated General Chemistry/ Toxicology/ Therapeutic drug monitoring Abbott Architect ci8200 Enzyme Immunoassay detection LP-BIO-P400 LP-BIO-P460 LP-BIO-P830 screening by immunoassay and confirmation by LCMSMS procedure, Urine, Urea, urine Uric Acid Urine Urine Protein Turbidmetric detection Valproate Vancomycin Urine 6-Monoacetyl Morphine Amphetamines Barbiturates Benzodiazepines Buprenorphine Cannabinoids Cocaine EDDP (methadone metabolite) Opiates Siemens CLINITEK Status LP-BIO-P715-Urinalysis and hcg assay by Siemens Clinitek Status Urine Urine Urinalysis (inc.: leukocytes, nitrites, urobilinogen, protein, ph, blood, specific gravity, ketones, bilirubin, glucose) β-human Chorionic Gonadotrophin (β-hcg) Colorimetric detection Lateral flow immunoassay, Urine Osmolality Micro-Osmometer 3320 Freezing point LP-BIO-P706_Osmolality assay by Advanced 3320 Microsmometer - Operations and procedures Assessment Manager: FN Page 3 of 10

4 BIOLOGICAL FLUIDS otherwise stated General Chemistry/ Toxicology/ Therapeutic drug monitoring Urine Ethyl glucuronide Waters Xevo TQ/ Waters Premier Urine Ethyl sulphate XE LCMSMS MRM LP-BIO-P912-Manual estimation of Ethylgucuronide by LCMSMS on Premier XE otherwise stated Immunochemistry and infectious serology Anti-tissue Transglutaminase (AntitTG) Phadia Immunocap P250 Sandwich ELFA detection LP-BIO-P717-Anti-tTG assay by Phadia Unicap P250 Faeces Calprotectin LP-BIO-P713-Calprotectin assay by Phadia Unicap P250 Assessment Manager: FN Page 4 of 10

5 BIOLOGICAL FLUIDS otherwise stated Immunochemistry and infectious serology Anti-cyclic citrinullated peptide (CCP) Anti-Hepatitis A IgM antibody (HAV IgM) Anti-Hepatitis B surface antibody (anti-hbs) Anti-Hepatitis B core antibody (anti- HBc) Anti-Hepatitis C Antibody (HCV) Abbott Architect isr2000 Sandwich CMIA detection LP-BIO-P400_Abbott Architect ci8200 Operational and Routine Maintenance procedures. LP-BIO-P460_Abbott Architect assay information chart Anti-Thyroid Peroxidase antibody (TPO) C-Peptide Hepatitis B surface antigen (HBsAg) Holotranscobalamin Human Immunodeficiency Virus Antibody/Antigen combo screening test (HIV Ag/Ab) Rubella IgG Syphilis serology Toxoplasma IgG Toxoplasma IgM Vitamin D (total) Assessment Manager: FN Page 5 of 10

6 BIOLOGICAL FLUIDS otherwise stated Immunochemistry and infectious serology Alphafoetoprotein (AFP) β-human Chorionic Gonadotrophin (β-hcg) Carbohydrate Antigen 12-5 (CA 12-5) Carbohydrate Antigen 153 (CA 153) Carbohydrate Antigen 19-9 (CA 19-9) Carcinoembryonic Antigen (CEA) Cortisol Dihydroandrostenedione sulphate (DHEA-S) Estradiol Ferritin Folate Follicle Stimulating Hormone (FSH) Free Triiodiothyronine (FT3) Free Thyroxine (FT4) Leutinising Hormone (LH) Progesterone Sex Hormone Binding Globulin Testosterone Thyroid Stimulating Hormone (TSH) Troponin I (High Sensitive) Vitamin B12 (Total) Anti-Mullerian Hormone (AMH)Parathyroid Hormone (intact) Prolactin Architect ci8200 Sandwich CMIA detection and FT4 using Competitive CMIA detection LP-BIO-P400_Abbott Architect ci8200 Operational and Routine Maintenance procedures. LP-BIO-P460_Abbott Architect assay information chart Beckman Coulter Access2 using sandwich CMIA detection and AMH using sandwich ELISA detection LP-BIO-P470_Beckman Coulter Access2 Operational and maintenance procedures LP-BIO-P670_Beckman Coulter Access 2 assay information chart Assessment Manager: FN Page 6 of 10

7 BIOLOGICAL FLUIDS otherwise stated Immunochemistry and infectious serology Faeces Elastase Launch Diagnostics DS2 Sandwich ELISA detection LP-BIO-P712_Faecal elastase ELISA by DS2 Biomerieux VIDAS 3 Sandwich-ELFA detection LP-BIO-P1010_Use of VIDAS 3 otherwise stated. NT-pro B-type Natriuretic Peptide (NT-proBNP) Varicella zoster IgG (VZG) Chromotography / Electrophoresis / other Alkaline phosphatase isoenzymes (APIS) Carbohydrate-Deficient Transferrin (CDT) LP-BIO-P710_NT-proBNP assay by Biomerieux VIDAS3 LP-BIO-P1016_VZV IgG assay by Biomeiuex VIDAS 3 SEBIA Capillarys Hydrasys & Hydrasys 2 Gel electrophoresis LP-BIO-P505 Alkaline Phosphatase Isoenzymes by Sebia HYDRASYS SEBIA Capillarys Flex 2 Capillary Zone Electrophoresis LP-BIO-P561- CDT by Capillarys FLEX Assessment Manager: FN Page 7 of 10

8 BIOLOGICAL FLUIDS otherwise stated Chromotography / Electrophoresis / Other protein electrophoresis: Paraprotein identification and quantitation of main protein fractions (albumin, alpha 1, alpha-2,, β-1, β-2, gamma fractions) protein electrophoresis - Isotype confirmation paraprotein identification and quantitation SEBIA Capillarys Flex 2 Capillary Zone Electrophoresis LP-BIO-P502- Electrophoresis by SEBIA Capillarys SEBIA Capillarys Flex 2/ Hydrasys & Hydrasys 2 Capillary Zone Electrophoresis - Immuno-typing /immunofixation LP-BIO-P504- Immunofixation (9 Samples) by SEBIA Hydrasys LP-BIO-P566 Immunofixation (9 samples) by SEBIA Hydrasys2 LP-BIO-P510 Immunotyping by SEBIA Capillarys Assessment Manager: FN Page 8 of 10

9 BIOLOGICAL FLUIDS otherwise stated Whole blood (lithium heparin) Chromotography / Electrophoresis / other Blood Gases (arterial/venous): ph, pco2, po2, Na +, K +, Cl -, Ca 2+, Haematocrit (PCV,) Glucose, Lactate, Total Bilirubin, Total Haemoglobin, Oxyhaemoglobin, Carboxyhaemoglobin, Methaemoglobin, Saturated Oxygen, Total Bicarbonate, Active Bicarbonate, Standard Bicarbonate, Base excess. IL GEM 4000 Indirect ISE (Ion Selective Electrode)/ Colorimetric LP-POC-P012-Operating the IL GEM 4000 Blood Gas Analyser CSF Haem pigments (Xanthachromia) Bio-C UVIKON Scanning spectrophotometry LP-BIO-P716-Xanthachromia assay by NorthStar Bio-C UVIKON spectrophotometer Methylmalonic acid (MMA) Waters Xevo TQ LC.MSMS MRM LP-BIO-P913-Estimation of Methylmalonic acid by LCMSMS Whole blood (K + EDTA) Haemaglobin A1c (HbA1c) SEBIA Capillarys Flex 2 Capillary Zone Electrophoresis LP-BIO-P567-Sebia Capillarys HbA1c Detailed procedure Assessment Manager: FN Page 9 of 10

10 BIOLOGICAL FLUIDS otherwise stated Chromotography / Electrophoresis / other Urine Bence Jones Protein SEBIA Capillarys Hydrasys & Hydrasys 2 Gel electrophoresis LP-BIO-P508 Urine Protein Electrophoresis by SEBIA Hydrasys LP-BIO-P563 Urine Protein Electrophoresis by SEBIA Hydrasys2 Urine Bence Jones Protein - Isotype confirmation SEBIA Capillarys Hydrasys & Hydrasys 2 Gel electrophoresis with immunofixation LP-BIO-P506 Urine Bence Jones Protein Immunofixation by SEBIA Hydrasys LP-BIO-P564-Urine BJP immunofixation by Hydrasys2 Vitamin D3/D2 Waters Premier XE/ Xevo TQ LC.MSMS MRM LP-BIO-P909-Automated 25 Hydroxyvitamin D3/D2 by SPE using LCMSMS LP-BIO-P Hydroxyvitamin D2 & D3 Estimation By LCMS/MS END Assessment Manager: FN Page 10 of 10

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