Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK University Hospital of South Manchester Biochemistry Department Wythenshawe Manchester M23 9LT Contact: Mr Brian Keevil Tel: +44 (0) Fax: +44 (0) Website: Testing performed at the above address only HUMAN BODY FLUIDS DETAIL OF ACCREDITATION Quantification of: Serum A1 Antitrypsin Turbidimetric assay Rheumatoid factor ACE Enzymatic assay A-ALT Alkaline Phosphatase Ammonia Amylase AST Cholesterol CK Gamma GT Glucose Lactate Lactate dehydrogenase Magnesium Phenytoin Total bile acids Triglyceride Urea Uric acid Serum and CSF Serum and Urine Glucose Protein Amylase Glucose Magnesium Urea Uric acid Assessment Manager: GS2 Page 1 of 8

2 HUMAN BODY FLUIDS (cont.) Quantification of: Serum Albumin Colourimetric BCP C3 Immunoturbidmetric C4 CRP Haptoglobin IgA IgG IgM Transferrin Urine Microalbumin Serum Phenytoin Enzyme immunoassay Theophylline Chloride Ion selective electrode, Potassium Sodium Serum and Urine Chloride Potassium Sodium Serum Creatinine Kinetic Alkaline Picrate Urine Serum and Urine 24hr creatinine clearance Creatinine Serum Direct bilirubin Diazo Total bilirubin Diazonium Salt Serum HDL Cholesterol Accelerator selective detergent/enzymatic Iron Ferene without protein removal step Serum and urine Macro Prolactin PEP extraction and CMIA Phosphate Phosphmolybdate Total protein Biuret Assessment Manager: GS2 Page 2 of 8

3 HUMAN BODY FLUIDS Urine Quantification of: 24 hr protein Serum Bicarbonate Oxidation of NADH Serum and Urine Calcium Colourimetric Architect i2000sr. Serum Alpha Feto protein Chemiluminescent microparticle Anti TPO Immunoassay (CMIA) B12 Ca125 Ca19-9 Ferritin Folate Free PSA Free T4 FSH FT3 High Sense Troponin 1 Insulin LH Oestradiol Progesterone Prolactin Prostate specific antigen PTH SHBG Total HCG TSH CEA Immunoassay Assessment Manager: GS2 Page 3 of 8

4 HUMAN BODY FLUIDS Serum Therapeutic Drug Monitoring/Antibiotic Amikacin Digoxin Gentamicin Tobramycin Valproate Vancomycin Carbamezapine Lithium Voriconazole Teicoplanin TPMT Toxicology examination activities for the purpose of clinical diagnosis Homogeneous particle-enhanced turbidimetric inhibition immunoassay (PETINIA). Enzyme immunoassay colourimetric BI-S-LC_VORI in conjunction with manufacturers instruction using /HPLC BI-S-LC-TPMT using /HPLC HPLC Chromatography Serum Ethanol Enzymatic assay Paracetamol Colourimetric/enzymatic Salicylate Assessment Manager: GS2 Page 4 of 8

5 HUMAN BODY FLUIDS (CONT.) Quantification of: PO-S-2 in conjunction with manufacturers instruction for analysis using Werfren Gem 4000 Blood Acid base balance Ion selective electrode analysis using /MS - Waters Acquity with XEVO family in conjunction with : Serum Aldosterone BI-S-LC-ALDO measurement by : Androstenedione BI-S-LCFTEST measurement by : Cortisol BI-S-LC-CORT measurement by : Cyclosporin Dexamethasone BI-S-LC-CYCTAC measurement by : BI-S-LC-CYCTAC measurement by : DHEAS Sulphate BI-S-LC-DHEAS measurement by : Everolimus BI-S-LC-SIREV measurement by : Androgen profile BI-S-LC-FTEST measurement by : Mycophenolic acid BI-S-LC-MPA measurement by : Plasma 5HIAA DOC 32 measurement by : Plasma Metanephrine BI-S-LC-PMETS measurement by : Prednisolone BI-S-LC-PRED measurement by : Assessment Manager: GS2 Page 5 of 8

6 HUMAN BODY FLUIDS Quantification of: analysis using /MS - Waters Acquity with XEVO family in conjunction with : Serum Renin BI-S-LC-RENIN measurement by : Sirolimus BI-S-LC-SIREV measurement by : Tacrolimus(FK506) BI-S-LC-CYCTAC measurement by : Testosterone BI-S-LC-FTEST measurement by : Vitamin A & E BI-S-LC-VITSCHRO measurement by Chromsystems kit for HPLC Serum Vitamin D metabolites BI-S-LC-VITD23 measurement by : Saliva Salivary Cortisol BI-S-LC_SFC measurement by : Testerone BI-S-LC-STEST measurement by : Urine Cystine BI-S-LC-UCYST measurement by : Free cortisol BI-S-LC-UFCOR measurement by : Citrate BI-S-ICIT measurement by : Cotinine BI-S-LC-UCOT measurement by : 5HIAA BI-S-LC-5HIAAMS measurement by : Assessment Manager: GS2 Page 6 of 8

7 HUMAN BODY FLUIDS analysis using Sebia Hydrasys in conjunction with : Serum Bence Jones Protein BI-S-PROT-UEPS measurement by electrophoresis analysis using Sebia capillarys electrophoresis in conjunction with : Serum Urine Albumin, Alpha1, Alpha 2, Beta, Gamma Calculated Parameter Adjusted calcium Globulin Free androgen index AKI Vitamin E/Cholesterol ratio Estimated GFR Urine creatinine clearance 24hr urine excretion calculation LDL and total cholesterol/hdl ratio Microalbumin/creatinine ratio BI-S-CAPILL-EPS Calculated using BI-S-AUTO-10 and BI-S-AUTO30 Calculated using BI-S-LC-FTEST Calculated using BI-S-AUTO-10 and BI-S-AUTO30 analysis using the Schebo manual Eliza with Thermo Multiskan FC Plate Reader with SkanIt software in conjunction with : Faeces Elastase BI-G-MISC-1 AND BI-S-MAN- ELAST Serum Assessment Manager: GS2 Page 7 of 8

8 HUMAN BODY FLUIDS Sweat and urine Osmolality Vitech osmometer. Depression of freezing point using BI-S- OSMOMETER Sweat Sweat Conductivity Analysis Documented in-house procedure BI-S-SWMD and BI-S-MISC-2 for analysis using Sherwood 926S chloride analyser. Measurement by Colourimetric titration Urine Pregnancy test - HCG Chromatographic immunoassay using Siemens Clinitek in conjunction with BI-S-MISC-8 CSF Xanthochromia Spectrophotometry using Thermo Scientific Evolution 300 spectrophotometer with OptLab- SPX software and Bilirubin application routine. END Assessment Manager: GS2 Page 8 of 8

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