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1 Ishrat Mahjabeen, PhD POSITION: Senior Scientific Officer (since Jan 2014) Department Of Biosciences COMSATS Institute of Information Technology, Islamabad PERSONAL DATA Father s name Din Muhammad Address khalid manzil, house # 126, ward #8, mohallah sadaat, Talagang Phone number , ACADEMIC QUALIFICATION 1. Ph.D., COMSATS Institute of Information Technology Islamabad in Major in Cancer Genetics. Thesis title Studies on Deregulation of Base Excision Repair Pathway Genes and micrornas in Head and Neck Cancer. 2. M.Phil. Quaid-e-Azam University Islamabad in Major in Developmental Biology. Thesis title Effect of Methylcobalamin on regeneration of crushed sciatic nerve of Rabbit. 3. MSc., University of Arid Agriculture Rawalpindi in Major in Zoology. Thesis title Effect of temperature on population of arthropods on Rosa Indica. AREA OF INTRESRT Cancer Genetics Molecular Genetics COURSES TAUGHT Research Techniques Page 1 of 12
2 RESEARCH EXPERTISE Light Microscopy and morphometric analysis Slide Preparation, Semi thin section cutting DNA and RNA tumor samples and cell lines Protein extraction from blood samples, tumor samples and cell lines PCR, Reverse transcriptase PCR and Real time PCR Sequencing Mutational analysis, Single stranded confirmation assay, Restriction fragment length polymorphism Protein structure prediction Cell culturing, Cell freezing and cell growth Assay Cell proliferation assay Cell migration assay Cell apoptosis assay Microarray MicroRNA profiling and TaqMan Assay Western Blotting, ELISA Immunohistochemistry and Flow Cytometry Training of graduate students and project designing in Lab COURSES STUDIED Molecular Biology Molecular Genetics Human Genetics Morphology Physiology Biochemistry Advances in Developmental Biology Advances in Reproductive Biology Advances in Human physiology Evolutionary Biology Taxonomy Ecology Advances in Molecular Biology Advances in Research Page 2 of 12
3 Research Report Zoogeography Andrology Wild life management Advances in Report writing DISTINCTIONS/AWARDS Gold medal for young scientist 2015 awarded by Pakistan Academy of Medical Sciences. Merit based scholarship at Govt Post Graduate college, Rawalpindi during FSc Merit based scholarship at QAU, Islamabad during M.Phil PhD, 5000 Indigenous Scholarship from HEC IRSP (HEC) six monthly Foreign Scholarship RESEARCH AND WORK EXPERIENCE 2014 to date: Senior Scientific Officer, Department of Biosciences, COMSATS Institute of Information Technology, Islamabad : PhD. Fellow Cancer Genetics Lab, Department of Biosciences, COMSATS Institute of Information Technology, Islamabad. October 2011-April 2012: Research Fellow Centre College of Dentistry, Center for Molecular Biology of Oral Diseases. University Of Illinois at Chicago, USA : Research Fellow Developmental Biology Lab, Quaid-e-Azam University, Islamabad : Research Fellow Entomology Lab, PMAS UAA Rawalpindi. One year honorary lectureship in Makkah System Of Education Talagang, ( ) Page 3 of 12
4 STATISTICS AND SOFTWARES Proficient use of window based software (Ms Word, Ms Excel, Ms PowerPoint). Data based software s operational skills including Statistical software (Statistica, SPSS, Pc-ORD, and MVSP). Graph pad prism Data Assist software for Real Time PCR data analysis Sequencing software Protein structure prediction software and other Bioinformatics tools (BLAST, primer3, ClustalW). CONFERENCES AND WORKSHOPS Poster presented at BICMAPE Science Conference, CIIT, Abbottabad. (July 28-29, 2009) Poster presented at 2 nd Annual Biosciences Poster Exhibition and Competition (BIOPEC), CIIT- Islamabad. (April, 2011). Poster presented at 1 st Annual Biosciences Poster Exhibition and Competition (BIOPEC), CIIT- Islamabad. (May, 2010). Sixth International Biennial Conferences of Microbiology, March 18-21, 2007, Quaid-i-Azam University, Islamabad. Page 4 of 12
5 PUBLICATIONS IN INERNATIONAL PEER REVIEWED JOURNALS 1. Mahjabeen I, Ali K, Zhou X, Kayani MA (2014) Deregulation of base excision repair gene expression and enhanced proliferation in head and neck squamous cell carcinoma. Tumour Biol. 35(6): doi: /s Mahjabeen. I, RM Baig, Sabir M and Kayani MA (2013) Genetic and Expressional variations of APEX1 are associated with increased risk of head and neck cancer. Mutagenesis. 28(2): Mahjabeen I, Baig RM, Masood N, Sabir M, Inayat U, Malik FA, Kayani MA (2013) Genetic variations in XRCC1 gene in sporadic head and neck cancer (HNC) patients. Pathology Oncology Research. Res. 19(2): Mahjabeen I, Chen Z, Zhou X, Kayani MA (2012) Decreased mrna expression levels of base excision repair (BER) pathway genes is associated with enhanced Ki-67 expression in HNSCC. Medical Oncology. 29: Mahjabeen I, Baig RM, Masood N, Sabir M, Inayat U, Malik FA, Kayani MA (2012) Novel mutations of OGG1 base excision repair pathway gene in laryngeal cancer patients. Familial Cancer. 11: Mahjabeen I, Baig RM, Masood N, Sabir M, Malik FA, Kayani MA (2011) OGG1 Gene Sequence Variation in Head and Neck Cancer Patients in Pakistan. Asian Pacific J Cancer Prev, 12, Page 5 of 12
6 7. Chen Z, Jin Y, YU D, Wang A, Mahjabeen I, Wang C, Liu X, Zhou X (2011) Down-regulation of the microrna-99 family members in head and neck squamous cell carcinoma. Oral oncology. 48(8): Baig RM, Mahjabeen I, Sabir M, Masood N, Ali K, Malik FA, Kayani (2013) Mutational spectrum of Gelsolin and Its down regulation is associated with breast cancer. Disease Marker Baig RM, Mahjabeen I, Sabir M, Masood N, Hafeez S, Malik FA, Kayani MA (2011) Genetic Changes in PTEN Gene and their Association with Breast Cancer in Pakistan. Asian Pacific Journal of Cancer Prevention Sabir M, Baig RM, Mahjabeen I, Kayani MA (2012) Novel germline CDK4 mutations in patients with head and neck cancer. Hereditary cancer in clinical practice 10: Sabir M, Baig RM, Mahjabeen I, Saeed M, Malik FA, Kayani MA (2012) Rb1/105 gene alterations and head and neck carcinogenesis. Molecular biology reports 39(10): Sabir M, Baig RM, Mahjabeen I, Kayani MA (2012) Significance of Cyclin B1 polymorphisms in patients with head and neck cancer. Accepted for publication in Internation journel of biological markers. 13. Masood N, Malik FA, Mahjabeen I, Baig RM, Kayani MA (2011) A novel CYP1A1 gene polymorphism and the risk of head and neck cancer in Pakistani population. African Journal of Biotechnology; 10 (27): Page 6 of 12
7 14. Masood N, Kayani MA, Malik FA, Mahjabeen I, Baig RM, Faryal R (2011) Genetic Variations in Carcinogen Metabolizing Genes Associated with Oral Cancer in Pakistani Population. Asian Pacific Journal of Cancer Prevention. 12: Masood N, Mahjabeen I, Malik FA, Baig RM, Kayani MA (2010) Association of GSTM1 and GSTT1 gene deletions with risk of head and neck cancer in Pakistan: a case control study. Asian Pacific Journal of Cancer Prevention. 11(4): Shakeel S, Mahjabeen I, Kayani MA, Faryal R (2013) Association of SYK genetic variations with breast cancer pathogenesis. Asian Pacific Journal of Cancer Prevention 14 (5), Wahid M, Mahjabeen I, Baig RM, Kayani MA (2013) Expression of CYP1A1 and GSTP1 in human brain tumor tissues in Pakistan. Asian Pac J Cancer Prev.14(12): Mahjabeen I, Kayani MA (2013). Novel Mutations of Apurinic/Apyrimidinic Endonuclease 1 Gene in Head and Neck Cancer Patients. International Journal of Clinical Toxicology, 1, Sabir M, Baig RM, Ali K, Mahjabeen I, Saeed M, Kayani MA (2014) Retinoblastoma (RB1) pocket domain mutations and promoter hyper-methylation in head and neck cancer. Cell Oncol (Dordr). 37(3): Page 7 of 12
8 20. Bashir N, Sana S, Mahjabeen I, Kayani MA (2014) Association of reduced XRCC2 expression with lymph node metastasis in breast cancer tissues. Fam Cancer. DOI: /s Ali K, Mahjabeen I, Sabir M, Baig RM, Faheem M, Kayani MA (2014) Germline variations of Apurinic/apyrimidinic Endonuclease 1 (APEX1) detected in female breast cancer Patients. Asian Pacific Journal of Cancer Prevention 15(18): Khan S, Mahjabeen I, Malik FA, Kayani MA (2014) Prognostic Significance of Altered Blood and Tissue Glutathione levels in Head and Neck cell Carcinoma. Asian Pacific Journal of Cancer Prevention 15(18): Qureshi Z, Mahjabeen I, Baig RM, Kayani MA (2014) Correlation between selected XRCC2, XRCC3 and RAD51 genes polymorphisms and primary breast cancer in women. Asian Pacific Journal of Cancer Prevention 15 (23): Kayani MA, Khan S, Baig RM, Mahjabeen I (2014) Association of RAD G/C, 172 G/T and XRCC3 Thr241Met gene polymorphisms with increased risk of head and neck cancer. Asian Pacific Journal of Cancer Prevention 15 (23), Ali K, Mahjabeen I, Sabir M, Mehmood H, Kayani MA (2015) OGG1 Mutations and Risk of Female Breast Cancer: Meta-Analysis and Experimental Data. Disease Markers. doi.org/ /2015/ Page 8 of 12
9 26. Shah A, Mahjabeen I, Kayani MA (2015) Genetic polymorphisms in cell cycle regulatory genes CCND1 and CDK4 are associated with susceptibility to breast cancer. B.U.ON. 20(4): Akhtar S, Mahjabeen I, Akram Z, Kayani MA (2015) CYP1A1 and GSTP1 gene variations in breast cancer, A systematic review and case-control study. Familial cancer. Familial cancer. DOI /s Zafeer M, Mahjabeen I, Kayani MA (2015) Increased expression of ERCC2 gene in head and neck cancer is associated with aggressive tumors; A systematic review and case-control study. International Journal of Biological Markers (accepted). 29. Mahjabeen I, Kayani MA (2015) Loss of mitochondrial tumor suppressor genes expression is associated with unfavorable clinical outcome in head and neck squamous cell carcinoma: data from retrospective study. Plos One (under-review) Page 9 of 12
10 BOOK CHAPTER Mahjabeen, Y. Jin, D. Chen, A. Wang, A. Kolokythas, X. Zhou. The role of microrna in head and neck cancer. In: Head & Neck Cancer: Current Perspectives, Advances, and Challenges, (Chapter 33) J.A. Radosevich, ed., ISBN , Springer (2013). RESEARCH SUPERVISION Graduates students supervised Saman Jahan Ansari (FA-11-BBS-012). Thesis title Studies on OGG1 polymorphism (ser326cys) in Thyroid Cancer Patients. Graduates students co-supervised AbidUllah Khan (FA-12-RBS-001). Thesis title Analysis of CCND1 and CDK4 variations in Breast Cancer Patients in Pakistani Population. Sumaira Akhtar (FA-12-RBS-023). Thesis title Analysis of Glutathione S- transferase PI and CYP1A1 variations in Breast cancer patients. Asad Ullah Khan (FA12-RBS-003). Thesis title Analysis of CYP2A6 and CYP2D6 genes in Head and Neck Cancer. Malik Waqar Ahmed (FA13-RBM-006). Thesis title Studies on expression of PTEN and proliferation marker Ki-67 in patients with head and neck cancer. Maryam Zafeer (FA13-RMG-002). Thesis title Expression deregulation of XPD/ERCC2 gene and proliferation marker Ki-67 in patients with head and neck cancer. Page 10 of 12
11 Project Awarded Analysis of XRCC1 Gene in Cancer Patients with HNC (0.2 millions) By CIIT ( ). Dr Ishrat Mahjabeen, Dr Mahmood Akhtar Kayani. Genetic, Epigenetic and Functional Analysis of Base Excision Repair Pathway Genes in Thyroid Cancer Patient. (3.1 millions) NRPU project HEC ( ) Dr Mahmood Akhtar Kayani, Dr Ishrat Mahjabeen. Arsenic Induced Genotoxicity in workers of different Industries of Pakistan (3.5 millions) NRPU project HEC ( ) Dr Zertashia Akram, Dr Ishrat Mahjabeen. Role Of microrna-145 in Regulation of Metastasis in Head And Neck Cancer by Targeting Magnesium Superoxide Dismutase 2 (SOD2) (1.0 millions) ISESCO Joint Research Grant Program Morocco (under review) Dr Ishrat Mahjabeen. Page 11 of 12
12 REFERENCES 1. Dr. Mahmood Akhtar Kayani, Professor, Department of Biosciences, COMSATS Institute of Information Technology Islamabad. Telephone #: Dr. Xiaofeng Zhou, Associate Professor, Center for Molecular Biology of Oral Diseases, University of Illinois at Chicago, USA. Telephone #: (312) Page 12 of 12
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