HPV-16 protein interactions. Ramya M Gundurao, Heather A Cubie, Jürgen Haas
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1 HPV-16 protein interactions Ramya M Gundurao, Heather A Cubie, Jürgen Haas
2 HPV lifecycle Woodman et al. Nature Reviews Cancer 7, (January 2007)
3 HPV lifecycle Woodman et al. Nature Reviews Cancer 7, (January 2007)
4 HPV lifecycle Woodman et al. Nature Reviews Cancer 7, (January 2007)
5 E7 Interactions: (Moody & Laimins, 2010)
6 RB E2F pathway RB E2F Cell cycle arrest CDK PP1c P P P P RB E2F Cell cycle progression
7 Aims To identify using yeast two-hybrid genomewide HPV protein interactions novel host binding partners of HPV
8 Yeast two-hybrid screen no protein interaction Prey Bait DBD X Y AD No transcription Preys Mating plate Diploid selection plate Interaction detection plates UAS REPORTER positive protein interaction X DBD Y AD Active transcription Baits UAS REPORTER X & Y proteins tested for interaction DBD - DNA binding domain AD - DNA activation domain UAS unique activation site
9 Results Genomewide screen Virus host screen
10 Intra-viral Screen Yeast two-hybrid screen Baits Preys Interaction detected and validated between E7 and E1(full length and N terminal 92 amino acids)
11 E1-E7 interaction mapping Myc tagged E7 deletion mutants CR1 CR2 CR3 E7 E7f4 E7f5 E7f6 E7f7 E7f8 normalised flurescence readout Interacting pair E1 interaction domain mapped to aa of E7
12 E7 site directed mutants 50HYNIVTFCCKC62 H51A Y52A N53A I54A V55A C59A C58A F57A T56A C61A K60A Normlised fluorescence readout Myc E EB Max E1 E1 E1 E1 E1 E1 E1 E1 E1 E1 E1 E1 EP Interacting pair E7 prb interaction CCKC (58-61aa) necessary for E1-E7 interaction In particular C58 and C61
13 HPV replication assay HPV replication assay 6 Fold change to pori M+E1+E water pori M only pori M + E1 pori M + E1 pori M + E1 pori M + E1 pori M + E1 pori M + E1 + E2 + E2 + E7 - + E2 + E7 + E2 + E7 + E2 + E7 1 ng C58A -1 ng C59A-1 ng C61A-1 ng Tranfected genes E7 downregulates HPV replication by 50% whereas E7 mutants unable to bind E1 do not inhibit replication in collaboration with Morgan Lab, Glasgow
14 Conclusions (1) HPV-16 E1 and E7 proteins interact N terminal 92 amino acids of E1 and zinc binding CCKC domain of E7 are necessary for interaction E7 might play an inhibitory role on E1 mediated HPV episomal replication
15 Genomewide screen Virus host screen
16 Virus host screen HPV proteins tested against human testis epithelial cell library 43 interactions identified for HPV proteins with cellular proteins
17 Virus host screen
18 LUMIER assay Normalised signal to background ratio FOS HPV-16 E7 HPV-16 E1^E2C HPV-16 L1 HPV 16 E1^E4* HPV 16 E6*E7* HPV-18 E1 JUN SPZ1 RPL36AL ADORA3 EEF1a1 KIFC3 SLC25A46 Interacting partners 6 out of 21 interactions tested were positive
19 SPZ1 Spermatogenic leucine zipper protein originally discovered in mouse testis (Hsu et al, 2001) member of Helix loop Helix zipper proteins increases cell proliferation and tumourigenesis by MAPK pathway Upregulates of Proliferative cell nuclear antigen PCNA (Hsu et al, 2005) interacts with protein phosphatase PP1cy2 and reduces its phosphorylase activity (Hrabchek & Vermuza,2004) PP1c dephosphorylates RB (Vietri et al, 2006) Does SPZ1 play a role in E7 mediated tumourigenesis?
20 SPZ1 expression in cervical cancer cell lines
21 SPZ1 binding domain on E7 E7 CR1 CR2 CR3 CR2 (15-37 amino acids) necessary for E7 interaction with SPZ1 which is also Rb interaction domain
22 Interaction network of SPZ1 PP1cγ SPZ1 PP1cα E7 Rb Published interactions Novel interactions
23 SPZ1 stabilises RB
24 Current Work Does SPZ1 block dephosphorylation of RB by PP1c? Does E7 reduce PP1c phosphorylase activity? Does E7 enhance PCNA upregulation by SPZ1? Do E7 from other HPV types interact with SPZ1? Is SPZ1 overexpressed in cervical cancer patients? Understanding the role of SPZ1 in cervical carcinogenesis
25 Acknowledgements Division of pathway medicine University of Edinburgh Jürgen Haas Annette Murr Members of Haas Group Scottish HPV reference lab Edinburgh Heather Cubie Kate Cushieri Maurice Canham Max-von-Pettenkofer Institute Ludwig-Maximilians University, Munich Susanne Bailer Marcus Heinzemann Morgan Lab, University of Glasgow Iain Morgan Mary Donaldson College of Medicine and Veterinary Medicine
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