Tissue: SC spp., Listeria monocytogenes, Campylobacter, Mannheimia

Transcription

1 New Jersey Animal Health Diagnostic Laboratory Courier Address: 3 Schwarzkopf Drive, Ewing, NJ USPS Address: PO Box 330, Trenton, NJ jerseyvetlab@ag.state.nj.us 2013 Fee Schedule Multiple Species Tests 1) Lung, placenta 1) Sw ab: ATMC Abortion bacteriology panel: Actinomyces pyogenes, Bacillus, Streptococcus $ days 2) Stomach contents spp., Listeria monocytogenes, Campylobacter, Mannheimia Acid fast stain $ day Aerobic culture $ days Anaerobic culture $ days 1) Feces 2) Bacterial Isolate 3) Sw ab 1) Sw ab 2) Tissue 1) Sw ab 2) Tissue 3) 3 ml fluid aspirates Anthrax screen $ days 1) Blood 2) Sw ab from bloody nose, mouth or rectum 3) Spleen or contaminated hide Antibiotic sensitivity/ Kirby-Bauer $ days 1) Sw ab from lesion 2) Bacterial isolate Antibiotic sensitivity/ minimum inhibitory concentration (MIC) $ days Fixed tissue(s) Biopsy/Histopathology (1-3 tissues) Pathology $ days Biopsy/Histopathology (4+ tissues) includes Necropsy in a bottle Pathology $ days Fixed tissues Biopsy/Histopathology, special stain Pathology $ days 1) Paraffin-embedded tissue block 2) Histology slide, unstained Brain removal Pathology $ days Whole head or carcass Brucellosis card Serology $ day 5 ml w hole blood or 2 ml serum RTT Brucella acidified plate (BAPA) Serology $ day 5 ml w hole blood or 2 ml serum RTT Brucella rivanol agglutination (RIV) Serology $ day 5 ml w hole blood or 2 ml serum RTT Campylobacter fetus ss fetus culture $ days 1) Abomasal fluid, lung, placenta 2) Aborted fetus 2) Agar slant/plate 3) ATMC 1) ATMC 1) Anaerobic transport media /Vacutainer 1) YTT 2) ATMC 1) Transport media 2) Agar slant/plate Suitable sealed container (plastic), 10% buffered formalin Suitable sealed container (plastic), 10% buffered formalin 1) Insulated package 2) Slide mailer 1) Sw ab: Clark s media Campylobacter jejuni culture $ days Feces, tied loop of intestine Sw ab: ATMC Carcass disposal (if >300 lbs. and if necropsy is performed in the laboratory) Pathology $0.20/lb NA Carcass Contact lab Caseous lymphadenitis culture $ days Lymph node, abscess/granuloma Sw ab: ATMC Caseous lymphadenitis synergistic hemolysin inhibition (SHI) titer $ days 3 ml w hole blood or 1 ml serum RTT Caseous lymphadenitis synergistic hemolysin inhibition (SHI) screen $ days 3 ml w hole blood or 1 ml serum RTT ***Containers: ATM/NC=Amies transport media no charcoal, ATMC=Amies transport media with charcoal, BHI=brain heart infusion, Clark s transport media=ctm, =double bagged, MTM=Mycoplasma transport media, PTT=purple top tube, RTT=red top tube, GTT=green top tube, SC=sterile container, VTM=viral transport media, WP=Whirl-pak, YTT=yellow top vacutainer Page 1 of 12

2 Multiple Species Tests, cont Clostridium perfringens culture $ days Feces, intestine SC Cyanide $ w eeks 10 ml w hole blood PTT Cytology Pathology $ days 1) Tissue 2) Stained/unstained slide 2) Slide mailer Diagnostic case consultation fee Pathology $55.00/hr 1-2 days Contact Lab Contact Lab Direct fungal smear $ day Scraping, hair SC Eastern equine encephalitis PCR Molecular $ days or CSF 2) Fresh or Frozen brain (preferably brain stem or medulla) Enteric bacteriology panel: Salmonella, E.coli, Clostridium; Johnes if ruminant $ days, Johnes takes 16 w ks E.coli O157 $ days Field necropsy (includes travel and procedure time only, see Necropsy for additional charges) Field sample collection (includes travel and procedure time only, see Test Name of interest for additional charges) Foot and mouth disease virus PCR Pathology $55.00/hour 8-10 days Pathology $23.00/hour 1-2 days Molecular $35.00/no cost if official investigation 2-3 days Post mortem samples: ligated intestinal loop, mesenteric lymph node Live animals: feces Feces, ligated loop of intestine (if fever) 2) Vesicle fluid, epithelia overlying vesicles, oro-pharyngeal fluid SC Sw ab: ATMC Fungal culture (Mycology) $ w eeks Hair, skin scraping SC Gram s stain $ day Heat/Ethanol fixed slide Slide mailer Influenza type A Molecular $ days Lung 2) VTM Sw ab: BHI, VTM Johnes ELISA Serology $ days 1 ml serum RTT Johnes PCR Molecular $ days Feces-4 grams Wide mouth tube Johnes solid culture $ w eeks Feces-4 grams Wide mouth tube Leptospira MAT paired serum $ days 2 ml serum RTT Leptospira MAT single serum $ days 2 ml serum RTT Listeria culture $ w eeks Brain, placenta, fetus, lung, SC abomasal fluid, silage Mycotoxins: Aflatoxins, Ochratoxin HPLC $50.00 each 1 w eek 1) 200g stomach / rumen contents, 1- Mycotoxins: DON/Vomitoxin, Fumoninsin, T-2 toxin, Zearalenone ELISA Necropsy: Abortion (1-4 aborted fetuses per dam w ith collective w eight < 100 lbs and placenta) includes gross exam, histopathology, aerobic culture, carcass disposal Necropsy: Abortion (1-3 aborted fetuses per dam w ith collective w eight lbs and placenta) includes: gross exam, histopathology, aerobic culture, carcass disposal toxin $25.00 each toxin 1 w eek Pathology $ days Pathology $ days 2) 100g feed 1) 200g stomach / rumen contents, 2) 100g feed s s 1- ***Containers: ATM/NC=Amies transport media no charcoal, ATMC=Amies transport media with charcoal, BHI=brain heart infusion, Clark s transport media=ctm, =double bagged, MTM=Mycoplasma transport media, PTT=purple top tube, RTT=red top tube, GTT=green top tube, SC=sterile container, VTM=viral transport media, WP=Whirl-pak, YTT=yellow top vacutainer Page 2 of 12

3 Multiple Species Tests, cont Necropsy: Cosmetic (small companion animals only): see Necropsy: Small Pathology $ days animal carcass (< 100 lbs.) Necropsy: Avian/Poultry (up to 5 birds) includes gross exam, histopathology, Pathology $ days aerobic culture, fecal parasite/ova identification, carcass disposal Necropsy: Avian/Poultry (up to 5 birds) includes gross exam, histopathology, aerobic culture, mycoplasma, avian influenza PCR, exotic New Castle Pathology $ days disease virus PCR, fecal parasite or ova ID, carcass disposal Necropsy: Avian Pet/Exotic (up to 3 birds) includes gross exam, Pathology $ days histopathology, aerobic culture, fecal parasite or ova ID, carcass disposal Necropsy: Large animal carcass (100 to 300 lbs.) includes gross exam, Pathology $ days histopathology, aerobic culture, fecal parasite or ova ID, carcass disposal Necropsy: Large animal carcass (>300 lbs.) includes gross exam, histopathology, aerobic culture, fecal parasite or ova ID. Additional charge Pathology $ days for disposal w ill be incurred at $0.20/lb charges apply) Necropsy: Additional fee for legal /insurance case Pathology $ Necropsy: Small animal carcass (< 100 lbs.) includes gross exam, histopathology, aerobic culture, fecal parasite or ova ID, carcass disposal Pathology $ days Necropsy: Spinal cord removal Pathology $ days, spinal column Delivered to AHDL or field necropsy ( Field necropsy Nitrogen, Nitrate, Nitrite $ w eeks 5 ml serum RTT (no gel separator) 1-2 w eeks 1) ml w hole blood 1) GTT Organochlorine pesticide & PCB s Screen $ rush) (Contact 2) 50 ml urine Lab for Parasite qualitative fecal flotation (parasite and ova ID) Pathology $ days min. 2 grams feces WP/SC Parasite quantitative fecal flotation (EPG) Pathology $ days min. 2 grams feces WP/SC Rabies FA Pathology 1) Brain $0.00-Brain 2-4 days 2) Head (Brain removed in the $32.00-Head laboratory) Respiratory bacteriology panel/large animal: Mannheimia haemolytica, Pasteurella multocida $17.00/isolate 2-4 days Salmonella culture $ days 1) Tracheal w ash 2) Lung 1) Fresh feces, ligated ileum, jejunum, or mesenteric LN, feed 2) Environmental sw ab 1) WP 2) in Styrofoam box w / ice 2) Sw ab: ATMC ***Containers: ATM/NC=Amies transport media no charcoal, ATMC=Amies transport media with charcoal, BHI=brain heart infusion, Clark s transport media=ctm, =double bagged, MTM=Mycoplasma transport media, PTT=purple top tube, RTT=red top tube, GTT=green top tube, SC=sterile container, VTM=viral transport media, WP=Whirl-pak, YTT=yellow top vacutainer Page 3 of 12 2) ATMC Salmonella drag sw ab culture $ days Drag sw ab ATMC Salmonella serogroup $ days Bacterial isolate Agar slant/plate Trace metals (Copper, Iron, Magnesium, Manganese, Molybdenum, Sodium, $25 per 3 ml w hole blood (heparin) GTT 1-2 w eeks Zinc, Mercury, Lead, Selenium, Arsenic) element

4 Multiple Species Tests, cont Trace metals (Copper, Iron, Magnesium, Manganese, Molybdenum, Sodium, Zinc, Mercury, Lead, Selenium, Arsenic) Transmissible Spongiform Encephalopathy (TSE) specimen collection, handling and submission ***Containers: ATM/NC=Amies transport media no charcoal, ATMC=Amies transport media with charcoal, BHI=brain heart infusion, Clark s transport media=ctm, =double bagged, MTM=Mycoplasma transport media, PTT=purple top tube, RTT=red top tube, GTT=green top tube, SC=sterile container, VTM=viral transport media, WP=Whirl-pak, YTT=yellow top vacutainer Page 4 of 12 $50 per element 1-2 w eeks Pathology $ days West Nile virus (WNV) PCR Molecular $ days Yersinia culture $ days Avian Tests Avian enteric bacteriology panel (individual or pooled specimens) includes Salmonella, E. coli and Clostridium Pathology $ days 200 g tissue SC Head, brain Kidney, brain, liver, heart, spleen Fresh feces, ileum, jejunum, mesenteric LN 1) 2) Cloacal or intestinal sw ab 3) Feces Avian influenza (AI) Agar(ose) gel immunodiffusion (AGID) Serology $ days 1 ml w hole blood or 1/2 ml serum RTT Avian influenza (AI) virus isolation Virology $ days 1) Tracheal, cloacal or oropharyngeal swab 2) Allantoic fluid Avian influenza (AI) matrix PCR Molecular $ days Avian paramyxovirus-1 (New Castle disease) PCR Molecular $ days Avian paramyxovirus-1 virulent New Castle disease (vndv) PCR Molecular $ days Avian paramyxovirus isolation Virology $ days 1) Tracheal, cloacal or oropharyngeal swab 2) Allantoic fluid 1) Tracheal, cloacal or oropharyngeal swab 2) Allantoic fluid 1) Tracheal, cloacal or oropharyngeal swab 2) Allantoic fluid Sw ab, tissues Sw ab: BHI Sw ab: ATMC 1) on ice 2) Anaerobic transport media 1) BHI 1) BHI 1) BHI 1) BHI Sw ab: BHI Tissues: SC Cytology: Blood, Crop or Trachea parasite examination/id Pathology $ day 1) 1) on ice 2) 3 smears (heat/ethanol fixed) 2) Slide mailer Eastern equine encephalitis (EEE) PCR Molecular $ days Kidney, brain, liver, heart, spleen SC Influenza virus H5 PCR Molecular $ days 1) Tracheal, cloacal or oropharyngeal swab 2) Allantoic fluid 1) BHI Influenza virus H7 PCR Molecular $ days 1) Tracheal, cloacal or oropharyngeal swab 2) Allantoic fluid Mycoplasma gallisepticum and M. synoviae Serology $ day 1 ml w hole blood or 1/2 ml serum RTT Necropsy: Avian Pet/Exotic (up to 3 birds) includes gross exam, histopathology, aerobic culture, fecal parasite or egg ID, carcass disposal Necropsy: Avian/Poultry (up to 5 birds) includes gross exam, histopathology, aerobic culture, fecal parasite/ova identification, carcass disposal Pathology $ days Pathology $ days 1) BHI

5 Avian Tests, cont Necropsy: Avian/Poultry (up to 5 birds) includes gross exam, histopathology, aerobic culture, mycoplasma, AI, END, fecal parasite or egg ID, carcass disposal Necropsy: Racing and Show Pigeons (up to 3 birds) includes gross exam, histopathology, aerobic culture (non-enteric), avian enteric bacteriology panel, qualitative fecal analysis, cytology (blood, crop, tracheal) Pigeon Virology PCR Panel (individual or pooled specimens) includes avian adenovirus, pigeon circovirus, pigeon herpesvirus, pigeon paramyxovirus-1 Pathology $ days Pathology $ days Molecular $ days Salmonella pullorum microtiter Serology $ days 1 ml w hole blood or 1/2 ml serum RTT West Nile virus PCR Molecular $ days Kidney, brain, liver, heart, spleen SC Bovine Tests Blue tongue virus AGID Serology $ days 1 ml w hole blood or 1/2 ml serum RTT Bovine abortion serology panel: BVD SN, IBR SN, lepto MAT, Brucellosis Bovine enteric panel: BVD SN/IBR SN/Johnes/E.coli/Clostridia/Campylobacter Virology/ Virology/ $ days $ days Bovine herpesvirus-1 (IBR) isolation Virology $ days 1 ml serum RTT 1) 1 ml serum AND 2) 4 gm feces Nasal/pharyngeal sw ab, lung, tracheal w ash, fetal tissue, placenta Bovine herpesvirus -1 serum neutralization (IBR) (SN) Virology $ days 1 ml serum RTT Bovine leukemia AGID Serology $ days 3 ml w hole blood or 1/2 ml serum RTT Bovine para-influenza-3 virus HI Virology $ days 1 ml serum RTT Bovine respiratory panel: IBR SN, parainfluenza-3 HI, BVD SN Virology $ days 1 ml serum RTT Bovine viral diarrhea (BVD) virus PCR Molecular $ days Bovine viral diarrhea (BVD) virus pooled PCR (up to 10 cattle) Molecular $ days 2) Serum (2 ml) (age 61+ days only) 3) Tissues (age 61+ days only) 2) Serum (2 ml) (age 61+days only) 3) Tissues (age 61+days only) Bovine viral diarrhea (BVD) virus serum neutralization Virology $ days 1 ml serum RTT Bovine viral diarrhea (BVD) virus isolation Virology $ days 2) Serum (2 ml) 3) Tissues Bovine mastitis including Mycoplasma sp. $ days; 7 days for my coplasma Milk quarter or bulk tank 1) RTT 2) WP or other SC Sw ab: RTT w /saline Wash: RTT MTM, SC Mastitis excluding Mycoplasma sp. $ days Milk quarter or bulk tank MTM, SC Uterine, cervical or vaginal mucous MTM, SC Mycoplasma culture (Bovine samples only) $ days or milk ***Containers: ATM/NC=Amies transport media no charcoal, ATMC=Amies transport media with charcoal, BHI=brain heart infusion, Clark s transport media=ctm, =double bagged, MTM=Mycoplasma transport media, PTT=purple top tube, RTT=red top tube, GTT=green top tube, SC=sterile container, VTM=viral transport media, WP=Whirl-pak, YTT=yellow top vacutainer Page 5 of 12

6 Camelid Tests Blue tongue virus AGID Serology $ days 2 ml w hole blood or 1/2 ml serum RTT Bovine viral diarrhea (BVD) virus PCR Molecular $ days Bovine viral diarrhea (BVD) virus pooled PCR (up to 2 camelids <61 days) Molecular $ days Bovine viral diarrhea (BVD) virus pooled PCR (up to 5 camelids 61+ days) Molecular $ days Bovine viral diarrhea (BVD) virus isolation Virology $ days Canine / Feline Tests Canine influenza PCR Molecular $ days 2) Serum (2 ml) (age 61+ days only) 3) Tissues (age 61+ days only) Whole blood 2) Serum (2 ml) 3) Tissues 2) Serum (2 ml) 3) Tissues 1) Nasal sw ab 2) Lung Canine influenza AGID Serology $ days 1 ml w hole blood or 1/2 ml serum RTT Canine influenza HI Virology $ days 1 ml serum RTT Feline herpesvirus-1 PCR Molecular $ days 1) Oronasal Sw ab 2) Oronasal biopsy, tissue Lyme ELISA & Western blot Virology $ days 1 ml serum RTT Caprine Tests Blue tongue virus AGID Serology $ days 2 ml w hole blood or 1/2 ml serum RTT Bovine viral diarrhea (BVD) PCR Molecular $ days 2) Serum (2 ml) 3) Tissues Caprine arthritis encephalitis virus AGID Serology $ days 3 ml w hole blood or 1/2 ml serum RTT Equine Tests Laboratory Additional fax/copy fee per official copy of equine infectious anemia Laboratory $2.00/fax 1 day certificate $1.00/pick up ***Containers: ATM/NC=Amies transport media no charcoal, ATMC=Amies transport media with charcoal, BHI=brain heart infusion, Clark s transport media=ctm, =double bagged, MTM=Mycoplasma transport media, PTT=purple top tube, RTT=red top tube, GTT=green top tube, SC=sterile container, VTM=viral transport media, WP=Whirl-pak, YTT=yellow top vacutainer Page 6 of 12 PTT 1) BHI/VTM/RTT w /saline 2) Sw ab: BHI/VTM 1) VTM/RTT w /saline Antibiotic sensitivity/ Kirby-Bauer $ days 1) Sw ab from lesion 1) Transport media 2) Bacterial isolate 2) Agar slant/plate Mare: sw ab from fossa sinus ATMC. Must arrive w ithin Contagious equine metritis for export/reproduction $20.00 per (cervix, uterus optional); Stallion: 48 hours on ice. Contact 7 days site/culture sw ab from fossa glandis, prepuce laboratory for pricing on and urethra import testing Eastern equine encephalitis (EEE) virus ELISA Virology $ days 1 ml serum RTT Eastern equine encephalitis (EEE) virus HI Virology $ days 1 ml serum RTT Eastern equine encephalitis (EEE) virus PCR Molecular $ days Equine abortion panel: EHV-1 SN/EVA SN/Leptospira MAT Paired Serum Virology/ $ days, CSF 2) Fresh or frozen brain (preferably brain stem or medulla) 1 ml serum RTT

7 Equine Tests, cont Equine enteric panel: Potomac horse fever IFA/Salmonella/E.coli/Clostridia 1) 1 ml serum for Potomac horse 1) RTT Serology/ $ days fever 2) Fresh feces Equine herpesvirus -1 (EHV-1) FA Virology $ days Fresh lung, liver, placenta, spinal SC/WP cord, adrenal gland Equine herpesvirus -1 (EHV-1) isolation Virology $ days Equine herpesvirus -1 PCR (genotyping):this test detects and differentiates respiratory and neurological strains of EHV-1 Molecular $50 for 1 horse $40.00/horse for 2 horses $35.00/horse for >2 horses 2 days 2) Nasal sw ab 3) Tracheal w ash, lung, liver, placenta, adrenal gland 2) Nasal sw ab 3) Tissue 2) BHI, saline or VTM /WP 2) BHI, saline or VTM Equine herpesvirus-1 (EHV-1) SN Virology $ days 1 ml serum RTT Equine infectious anemia AGID Serology $ day 3 ml w hole blood or 1/2 ml serum RTT Equine infectious anemia ELISA Serology $20.00 Same day 3 ml w hole blood or 1/2 ml serum RTT Equine influenza virus isolation Virology $ days Nasal sw ab BHI or VTM Equine influenza HI (includes Miami, Prague, Kentucky and Alaskan strains) Virology $ days 1 ml serum RTT Equine neurological panel: EHV-1 SN/WNV IgM ELISA/ EEE IgM ELISA Virology $ days 1 ml serum RTT Equine respiratory panel: EHV-1 SN, EVA SN, equine influenza HI Virology $ days Equine viral arteritis (EVA) virus isolation Virology $ days Lung, placenta, semen, fetal tissues, lymph node 2) Semen 3) Pharyngeal sw ab 4) Tissue Equine viral arteritis (EVA) SN Virology $ days 1 ml serum RTT Lyme ELISA & Western blot Serology $ days 1 ml serum RTT Potomac horse fever IFA $ days 1 ml serum RTT Potomac horse fever PCR Molecular $ days 2) Cecal/colonic mucosal scrapping 1) Nasal, pharyngeal, tracheal, Streptococcus equi sub species PCR: This test detects & differentiates S. guttural pouch, abscess swab Molecular $ days equi subsp equi (strangles) & S. equi subsp zooepidemicus 2) Nasal, pharyngeal, tracheal, guttural pouch w ash, CSF West Nile virus (WNV) PCR Molecular $ days 2) Fresh or frozen of brain (preferably brain stem or medulla) West Nile virus (WNV) ELISA-IgM Virology $ days 1 ml serum RTT West Nile virus (WNV) ELISA-IgG Virology $ days 1 ml serum RTT Western equine encephalitis (WEE) virus HI Virology $ days 1 ml serum RTT WP, SC 2) Semen straw 3) VTM 4) SC 1) Moistened cotton or Dacron ***Containers: ATM/NC=Amies transport media no charcoal, ATMC=Amies transport media with charcoal, BHI=brain heart infusion, Clark s transport media=ctm, =double bagged, MTM=Mycoplasma transport media, PTT=purple top tube, RTT=red top tube, GTT=green top tube, SC=sterile container, VTM=viral transport media, WP=Whirl-pak, YTT=yellow top vacutainer Page 7 of 12

8 Ovine Tests Blue tongue virus AGID Serology $ days 2 ml w hole blood or 1/2 ml serum RTT Bovine viral diarrhea (BVD) virus PCR Molecular $ days 2) Serum (2 ml) 3) Tissues Ovine progressive pneumonia virus AGID Serology $ days 3 ml w hole blood or 1/2 ml serum RTT Piscine Tests $17.00/fish 1) Sw ab 1) ATMC Aerobic bacterial culture (fresh water species) or $ ) Tissue 2 to 5 days for 12 pools 3) Whole fish, on ice 3) Leak proof w ith (5 fish ea.) Antibiotic sensitivity (Kirby Bauer) $ to 4 days Bacterial isolate Agar slant/plate Bacterial isolate identification $12.00 per Bacterial isolate Agar slant/plate 2-5 days isolate Bacterial kidney disease (Renibacterium salmoninarum) Enteric red mouth (Yersinia rucekri) Culture up to 5 fish of same species pooled Test under development $17.00/fish or $70.00 for 12 pools (5 fish ea.) NA 5 days External parasite cytology (fin and gill clips, skin scrape) Pathology $ days Fish health inspection tissue collection only (in accordance with USFWS AFS-FHS Blue Book and/or OIE guidelines) Fish health inspection (in accordance with USFWS AFS-FHS Blue Book and/or OIE guidelines) Furunculosis (Aeromonas salmonicida) Culture up to 5 fish of same species pooled Pathology Pathology $1.00 per fish Contact lab for pricing $17.00/fish or $70.00 for 12 pools (5 fish ea.) Histopathology (1-5 fish) Pathology $ day Up to 1 month 5-20* days (*if atypical strains) Up to 7 days 1) Whole fish, on ice 2) Frozen posterior kidney 3) Fixed posterior kidney Whole fish, on ice 1) Whole fish on ice 2) Fixed tissues Whole fish, on ice Whole fish, on ice Whole fish, on ice Fixed tissues Infectious haematopoietic necrosis (IHN) virus RT-PCR Virology $ days Cell culture only 1) <4cm: w hole fish on ice $20.00 per 2) 4-6cm: enitre viscera Infectious haematopoietic necrosis (IHN) virus Isolation sample Up to 21 Virology 3) >6cm; kidney, spleen up to 5 fish of same species pooled (pool) per days 4) Sexually mature: ovarian fluid, cell line kidney, spleen, encephalon Infectious pancreatic necrosis (IPN) virus RT-PCR Virology $ days Cell culture only 1-2) Leak proof w ith 3) Suitable sealed container (plastic), 10% buffered formalin Leak proof w ith refrigerant 1) Leak proof, refrigerant 2) Suitable sealed container (plastic), 10% buffered formalin Leak proof, refrigerant Leak proof, refrigerant Leak proof w ith refrigerant Suitable sealed container (plastic) 10% buffered formalin 1-2) Leak proof w ith 3-4) VTM ***Containers: ATM/NC=Amies transport media no charcoal, ATMC=Amies transport media with charcoal, BHI=brain heart infusion, Clark s transport media=ctm, =double bagged, MTM=Mycoplasma transport media, PTT=purple top tube, RTT=red top tube, GTT=green top tube, SC=sterile container, VTM=viral transport media, WP=Whirl-pak, YTT=yellow top vacutainer Page 8 of 12

9 Piscine Tests, cont 1) <4cm: w hole fish on ice 1-2) Leak proof w ith $20.00 per 2) 4-6cm: enitre viscera Infectious pancreatic necrosis (IPN) virus Isolation sample Up to 21 Virology 3) >6cm; kidney, spleen 3-4) VTM up to 5 fish of same species pooled (pool) per days 4) Sexually mature: ovarian fluid, cell line kidney, spleen, encephalon Koi herpesvirus (KHV) PCR Virology $ days Mycobacteria spp. PCR for aquatic isolates w ith species identification $ plus shipping days Necropsy (1-5 fish) includes gross exam, histopathology, special stains Pathology $ days 1) Whole fish on ice 2) Kidney, spleen, gill filaments 1) Frozen tissue 2) Paraffin embedded tissue block 3) Culture Whole fish on ice Spring viremia of carp (SVC) RT-PCR Virology $ days Cell culture only 1) <4cm: w hole fish on ice $20.00 per 2) 4-6cm: enitre viscera sample Up to 21 Spring viremia of carp (SVC) virus isolation Virology 3) >6cm; kidney, spleen (pool) per days 4) Sexually mature: ovarian fluid, cell line kidney, spleen, encephalon Viral hemorrhagic septiciemia (VHS) RT-PCR Virology $ days Cell culture only $20.00 per sample (pool) per 1) <4cm: w hole fish on ice 2) 4-6cm: enitre viscera 3) >6cm; kidney, spleen Viral hemorrhagic septiciemia (VHS) virus isolation Virology surveillance grant cell line. No cost if covered by Up to 21 days 4) Sexually mature: ovarian fluid, kidney, spleen, encephalon Virus isolation up to 5 fish of same species pooled Whirling disease (Myxobolus cerebralis) P.Centrifuge method up to 5 fish of same species pooled Porcine Tests Virology $20.00 per sample per cell line $ for 12 pools (5 fish ea.) $35.00 / no cost if official surveillance 1) Leak proof; Fish on 2) Sterile saline or VTM 1-2) Leak proof w ith 3) Agar slant/plate Leak proof, refrigerant 1-2) Leak proof w ith 3-4) VTM 1-2) Leak proof w ith 3-4) VTM ***Containers: ATM/NC=Amies transport media no charcoal, ATMC=Amies transport media with charcoal, BHI=brain heart infusion, Clark s transport media=ctm, =double bagged, MTM=Mycoplasma transport media, PTT=purple top tube, RTT=red top tube, GTT=green top tube, SC=sterile container, VTM=viral transport media, WP=Whirl-pak, YTT=yellow top vacutainer Page 9 of 12 Up to 21 days 7 days Check guidelines for specific viruses Whole fish: Leak proof w ith Tissue: VTM 60 fresh fish or fish heads Leak proof w ith refrigerant Whole blood of febrile animal, tonsil Classical sw ine fever (Hog cholera) Molecular 2-3 days Porcine resp. and repro. syndrome (PRRS) IFA Virology $ days 1 ml serum RTT Pseudorabies virus latex agglutination Virology Contact lab 1 ml serum RTT Sw ine influenza HI Virology $ days 1 ml serum RTT Brucellosis card Serology $ day 5 ml w hole blood or 2 ml serum RTT Brucella acidified plate (BAPA) Serology $ day 5 ml w hole blood or 2 ml serum RTT Brucella rivanol agglutination (RIV) Serology $ day 5 ml w hole blood or 2 ml serum RTT Sw ine influenza PCR Molecular $ days 1) Nasal Sw ab 2) Lung Tonsil: WP/SC Blood: PTT 1) Viral transport media 2) WP/SC

10 Zoo, Wildlife and Exotic Animal Tests Abortion bacteriology panel: Actinomyces pyogenes, Bacillus, Streptococcus spp., Listeria monocytogenes, Campylobacter, Mannheimia $ days Acid fast stain $ day Acid fast stain, Cryptosporidium Pathology $ days Aerobic culture $ days Anaerobic culture $ days Anthrax screen $ days 1) Lung, placenta 2) Stomach contents 1) Feces 2) Bacterial isolate 3) Sw ab or rectal mucosal scrape Cytology from feces or gastric mucosal scraping (heat/ethanol fixed) 1) Sw ab 2) Tissue 1) Sw ab 2) Tissue 3) 3 ml fluid aspirates 1) Blood 2) Sw ab from bloody nose, mouth or rectum 3) Spleen or contaminated hide 1) Sw ab from lesion 2) Bacterial isolate 1) Sw ab: ATMC 2) Agar slant/plate 3) ATMC Slide holder 1) ATMC 1) Anaerobic transport media /Vacutainer 1) YTT 2) ATMC Antibiotic sensitivity/ Kirby-Bauer $ days 1) Transport media 2) Agar slant/plate Antibiotic sensitivity/ minimum inhibitory concentration (MIC) $ days Bacterial isolate Agar slant/plate Avian cholera (Pasturella multocida) $ days 1) Choanal / Sinonasal sw ab 1) Culturette 2) Heart, liver and w ing Avian influenza (AI) AGID Serology $ days 1 ml w hole blood or ½ ml serum RTT Biopsy/Histopathology (1-3 tissues) Pathology $ days Fixed tissue(s) Suitable sealed container (plastic), 10% buffered formalin Biopsy/Histopathology (4+ tissues) includes Necropsy in a bottle Pathology $ days Fixed tissues Suitable sealed container (plastic), 10% buffered formalin Biopsy/Histopathology, special stain Pathology $ days 1) Paraffin-embedded tissue block 1) Insulated package 2) Histology slide, unstained 2) Slide mailer Bovine viral diarrhea (BVD) virus PCR Molecular $ days 2) Serum 3) Tissues 1) PT Cytology: Blood or Crop parasite examination/id Pathology $ day Eastern equine encephalitis (EEE) PCR Molecular $ days 1) 2) 3 blood or crop smears (heat/ethanol fixed), CSF 2) Fresh or frozen brain (preferably brain stem or medulla) 1) on ice 2) Slide mailer 1) RTT ***Containers: ATM/NC=Amies transport media no charcoal, ATMC=Amies transport media with charcoal, BHI=brain heart infusion, Clark s transport media=ctm, =double bagged, MTM=Mycoplasma transport media, PTT=purple top tube, RTT=red top tube, GTT=green top tube, SC=sterile container, VTM=viral transport media, WP=Whirl-pak, YTT=yellow top vacutainer Page 10 of 12

11 Zoo, Wildlife and Exotic Animal Tests, cont Enteric bacteriology panel: Salmonella, E.coli, Clostridium; Johnes if ruminant $45.00 ***Containers: ATM/NC=Amies transport media no charcoal, ATMC=Amies transport media with charcoal, BHI=brain heart infusion, Clark s transport media=ctm, =double bagged, MTM=Mycoplasma transport media, PTT=purple top tube, RTT=red top tube, GTT=green top tube, SC=sterile container, VTM=viral transport media, WP=Whirl-pak, YTT=yellow top vacutainer Page 11 of days, Johnes takes 16 w ks External parasite ID Pathology $ days Post mortem samples: ligated intestinal loop, mesenteric lymph node Live animals: 100g feces Ectoparasite, w hole in ethanol solution Field/Zoo/Aquarium necropsy (includes travel time only, see Necropsy for Pathology $55.00/hour 2 days additional fees) Field sample collection Pathology $23.00/hour 2-3 days (if fever) $35/no cost 2) Vesicle fluid, epithelia overlying Foot and mouth disease virus PCR Molecular if official 2-3 days vesicles, esophageal-pharyngeal investigation fluid SC Leak proof vial Fungal culture (Mycology) $ w eeks Hair, skin scraping SC Gram Stain $ day heat fixed slide Slide mailer Influenza type A Molecular $ days Lung 2) VTM Sw ab: BHI, VTM Johnes ELISA Serology $ days 1 ml serum RTT Johnes PCR Molecular $ days Feces-4 grams Wide mouth tube Johnes solid culture $ w eeks Feces-4 grams Wide mouth tube Leptospira MAT paired serum $ days 2 ml serum RTT Leptospira MAT single serum $ days 2 ml serum RTT Lyme ELISA & Western blot Virology $ days 1 ml serum RTT Mycotoxins: Aflatoxins and Ochratoxin HPLC Mycotoxins: DON/Vomitoxin, Fumoninsin, T-2 toxin, Zearalenone ELISA $50.00 each toxin $25.00 each toxin Organochlorine pesticide & PCB s $ w eek 1 w eek 1-2 w eeks Contact Lab for rush 1) 200g stomach / rumen contents 2) 100g feed 1) 200g stomach / rumen contents 2) 100g feed 1) ml w hole blood (heart blood if dead) 2) 50 ml urine Parasite qualitative fecal flotation Pathology $ days min. 2 grams feces WP, SC 1) Brain $0.00-Brain Rabies Pathology 2-4 days 2) Head (Brain removed in the $32.00-Head laboratory) Respiratory bacteriology panel/large animal: Mannheimia haemolytica, Pasteurella multocida $17.00/isolate 2-4 days Salmonella culture $ days Tracheal w ash Lung 1) Fresh feces, ligated ileum, jejunum, or mesenteric LN, feed 2) Environmental sw ab ) GTT 1) WP 2) in Styrofoam box w / ice 2) Sw ab: ATMC 2) ATMC Salmonella drag sw ab culture $ days Drag sw ab ATMC Salmonella serogroup $ days Bacterial isolate Agar slant/plate Salmonella pullorum microtiter Serology $ days 1 ml w hole blood or ½ ml erum RTT

12 Zoo, Wildlife and Exotic Animal Tests, cont Trace Metals (Copper, Iron, Magnesium, Manganese, Molybdenum, Sodium, $25 per 3 ml w hole blood (heparin) GTT 1-2 w eeks Zinc, Mercury, Lead, Selenium, Arsenic) element Trace Metals (Copper, Iron, Magnesium, Manganese, Molybdenum, Sodium, $50 per 200 g tissue SC 1-2 w eeks Zinc, Mercury, Lead, Selenium, Arsenic) element West Nile virus (WNV) PCR Molecular $ days Kidney, brain, liver, heart, spleen SC *The New Jersey Department of Agriculture is permitted by law to charge those fees for animal disease diagnostic and testing services which are necessary for the Department to recover the costs incurred for materials, equipment, test kits, reagents, and some associated personnel expenses for these testing services. Interpretation or utilization of laboratory results in the context of animal or herd health to provide services like treatment to cure diseases, vaccinations to prevent diseases, issuance of health certificates and the like are within the practice of veterinary medicine and therefore done by licensed veterinarians as provided in the Veterinary Practice Act. Licensed veterinarians may charge for their services. ***Containers: ATM/NC=Amies transport media no charcoal, ATMC=Amies transport media with charcoal, BHI=brain heart infusion, Clark s transport media=ctm, =double bagged, MTM=Mycoplasma transport media, PTT=purple top tube, RTT=red top tube, GTT=green top tube, SC=sterile container, VTM=viral transport media, WP=Whirl-pak, YTT=yellow top vacutainer Page 12 of 12