SCOPE OF ACCREDITATION TO ISO/IEC 17025:2005
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1 SCOPE OF ACCREDITATION TO ISO/IEC 17025:2005 NATIONAL VETERINARY SERVICES LABORATORIES USDA-APHIS-VS-NVSL DIAGNOSTIC BACTERIOLOGY LABORATORY PATHOBIOLOGY LABORATORY DIAGNOSTIC VIROLOGY LABORATORY 1920 Dayton Avenue Ames, Iowa Dr. Karl Hochstein BIOLOGICAL Valid to: June 30, 2019 Certificate Number: In recognition of the successful completion of the A2LA evaluation process, accreditation is granted to this laboratory to perform testing on animal sera, whole blood, plasma, tissues, fluids, and environmental samples: Tests Test Methods Diagnostic Bacteriology Laboratory Serology Agar Gel Immunodiffusion Johne s Mycobacterium avium, subsp. Paratuberculosis Dourine Trypanosoma equiperdum SOP-SERO-0045 SOP-SERO-0007 Agglutination Brucellosis Brucella abortus/suis (Tube) SOP-SERO-0019 B. abortus/suis (Plate) SOP-SERO-0016 B. abortus/suis (BAPA) SOP-SERO-0017 B. abortus/suis (RAP) SOP-SERO-0021 B. abortus/suis (Rivanol) SOP-SERO-0018 B. abortus/suis (Card) SOP-SERO-0020 B. canis (ME Tube) SOP-SERO-0024 B. melitensis (Card) SOP-SERO-0027 Equine paratyphoid Salmonella abortus SOP-SERO-0009 Tuleremia Francisella tularensis SOP-SERO-0028 Complement Fixation Test Anaplasmosis Anaplasma marginale Bovine babesiosis Babesia bigemina & Babesia bovis SOP-SERO-0010 SOP-SERO-0013 (A2LA Cert. No ) 11/20/2017 Page 1 of 9
2 Brucellosis Brucella abortus/suis SOP-SERO-0015, B. ovis SOP-SERO-0026 Contagious Equine Metritis Taylorella equigenitalis SOP-SERO-0039, SOP-SERO-0022 Dourine Trypanosoma equiperdum SOP-SERO-0006 Glanders Burkholderia mallei SOP-SERO-0008 Johne s Mycobacterium avium subsp. Paratuberculosis SOP-SERO-0012 Piroplasmosis Babesia caballi & Theileria equi SOP-SERO-0003 Dual Path Platform (Lateral Flow) Tuberculosis Mycobacterium bovis SOP-SERO-0044 ELISA Anaplasmosis Anaplasma Marginale SOP-SERO-0029 Brucellosis Brucella abortus/melitensis SOP-SERO-0023 B. ovis SOP-SERO-0035 B. canis SOP-SERO-0025 Equine Piroplasmosis Babesia caballi & Theileria equi (In-house) SOP-SERO-0005 B. caballi & T. equi (Kit) SOP-SERO-0032 Johne s Mycobacterium avium subsp. Paratuberculosis SOP-SERO-0033 Tuberculosis Mycobacterium bovis SOP-SERO-0041 Fluorescence Polarization Assay (FPA) Brucella abortus/suis (Tube & Plate) Gamma Interferon Test Tuberculosis Mycobacterium TB Complex (Bovigam) Indirect Fluorescent Antibody Piroplasmosis Babesia caballi & Theileria equi Western Blot Piroplasmosis Babesia caballi SOP-SERO-0030 SOP-SERO-0031 SOP-SERO-0051 SOP-SERO-0004 SOP-SERO-0040 (A2LA Cert. No ) 11/20/2017 Page 2 of 9
3 Diagnostic Bacteriology Laboratory Bacterial Identification Antibiotic Susceptibility Agar Gel Immunodiffusion Agglutination Leptospira (Microagglutination) Mycoplasma Mycoplasma (Hemagglutination iinhibition) Salmonella Pullorum and Typhimurium Bacterial Identification MALDI-Biotyper 16S rdna Sequencing SOP-BI-0010 SOP-BI-0036 SOP-BI-0038 SOP-BI-0079 SOP-BI-0078 SOP-BI-0015, SOP-BI-0016 SOP-BI-0017 SOP-BI-0115 SOP-BI-0113 PCR Campylobacter fetus fetus and C. fetus venerealis SOP-BI-0012 rt-pcr Taylorella spp. Isolation and Identification General Bacteriology Bacillus anthracis Burkholderia mallei & B. pseudomallei Francisella tularensis Taylorella spp. Pulsed Field Gel Electrophoresis (PFGE) Salmonella Serotyping Salmonella SOP-BI-0109 SOP-BI-0075 SOP-BI-0200, SOP-BI-0201, SOP-BI-0202 SOP-BI-0204 SOP-BI-0205, SOP-BI-0206, SOP-BI-0207 SOP-BI-0106, SOP-BI-0109 SOP-BI-0025 SOP-BI-0021, SOP-BI-0022 Diagnostic Bacteriology Laboratory MB Bacterial Isolation Brucella spp. Mycobacterium spp. Culture Solid Media Phenotypic Identification Brucella spp. Biochemical Tests (CO 2, H e SO 4, Cat +, Inhibition of Dyes/ Additives/Rifampicin, Urease, MAT, Tb Phage) Mycobacterium spp. SOP-MB-0009 SOP-MB-0039, SOP-MB-0059 SOP-MB-0022, SOP-MB-0041 SOP-MB-0042, SOP-MB-0045 SOP-MB-0040, SOP-MB-0057 SOP-MB-0010, SOP-MB-0019 (A2LA Cert. No ) 11/20/2017 Page 3 of 9
4 Microscopy (Acid-fast Staining) Molecular Identification Brucella spp. Confirmatory PCR - AMOS Mycobacterium spp. Direct PCR - M. bovis, MAP, MTBC Confirmatory PCR - MTBC Differential, MAP Sequencing - 16s rdna ITS, rpob, hsp65 Genomic Characterization Microsatellite Bovine Identity Testing Ovine Identity Testing Sequencing Scrapie Susceptibility Whole Genome Sequencing Nextera XT DNA Library Preparation for Whole Genome Sequencing of Bacterial Isolates SOP-MB-0028, SOP-MB-0037 SOP-MB-0017, SOP-MB-0019 SOP-MB-0046, SOP-MB-0079, SOP-MB-0080 SOP-MB-0052 SOP-MB-0031 SOP-MB-0060 SOP-MB-0076 SOP-MB-0074 SOP-MB-0075 Diagnostic Virology Laboratory Virus Isolation Avian Influenza Newcastle Disease Avian Adenovirus Avian Pox Avian Reovirus Infectious Bursal Disease Infectious Laryngotracheitis Vesicular Stomatitis Isolation of Viral Agents from Finfish in Tissue Cell Culture Swine Influenza Virus Encephalomyocarditis Virus Hemagglutinating Encephalomyelitis Virus Porcine Circovirus Porcine Parvovirus Porcine Reovirus Porcine Respiratory Coronavirus Porcine Rotavirus Porcine Rubularvirus Porcine Sapeloirus (Enterovirus A) Porcine TeschovirusTransmissible Gastroenteritis Virus Viral Abortion (Porcine) Pseudorabies Virus Porcine Epidemic Diarrhea Porcine Deltacorona Virus Porcine Reproductive and Respiratory Syndrome Bovine Coronavirus Bovine Enterovirus Bovine Herpesvirus 1 (Infectious Bovine Rhintracheitis) Bovine Herpesvirus 2 (Bovine Herpes Mammillitis) SOP-AV-0018 SOP-AV-0019, SOP-AV-0022 SOP-AV-0018, SOP-AV-0019 SOP-BPA-0005 SOP-BPA-0014 SOP-BPA-0023 SOP-BPA-0024 SOP-BPA-0021, SOP-BPA-0024 SOP-BPA-0024, WI-BPA-0041 SOP-BPA-0045 SOP-BPA-0046 (A2LA Cert. No ) 11/20/2017 Page 4 of 9
5 Bovine Herpesvirus 4 Bovine Papular Stomatitis Bovine Parvovirus Bovine Respiratory Syncytial Virus Bovine Rotavirus Bovine Viral Diarrhea Pseudocowpox Viral Abortion (Bovine) Bluetongue Epizootic Hemorrhagic Disease Equine Viral Arteritis Eastern Equine Encephalomyelitis Western Equine Encephalomyelitis Venezuelan Equine Encephalomyelitis West Nile Virus Equine Influenza Equine Adenovirus Equine Herpesvirus 1 Equine Herpesvirus 2 Equine Herpesvirus 3 Equine Herpesvirus 4 Equine Rhinitis Virus African Horse Sickness Isolation / Indirect Fluorescent Assay (IFA) Chlamydophila psittaci Indirect Fluorescent Assay (IFA) Avian Encephalomyelitis Infectious Laryngotracheitis Avian Metapneumovirus Avian Pox Avian Reovirus Duck Viral Enteritis Psittacine Herpesvirus Bovine Coronavirus Bovine Herpesvirus 4 Bovine Papular Stomatitis Bovine Parvovirus Bovine Rotavirus Porcine Reovirus Porcine Rotavirus Pseudocowpox Porcine Reproductive and Respiratory Syndrome Porcine Sapeloirus (Enterovirus A) Porcine Teschovirus Immunoperoxidase Test (IPT) / Peroxidase Linked Assay (PLA) Malignant Catarrhal Fever SOP-EO-0008 SOP-EO-0009 SOP-EO-0010 SOP-EO-0011 SOP-EO-0012 SOP-EO-0013 SOP-EO-0014 SOP-AV-0020 SOP-AV-0026, SOP-AV-0050 SOP-BPA-0047 SOP-BPA-0031 SOP-BPA-0050 SOP-BPA-0020 (A2LA Cert. No ) 11/20/2017 Page 5 of 9
6 Polymerase Chain Reaction (PCR) Avian Influenza (rrt-pcr) Newcastle Disease (rrt-pcr) Avian Influenza (Additional Platforms) Swine Influenza Virus (rrt-pcr) Malignant Catarrhal Fever (PCR) Bovine Viral Diarrhea (PCR) Porcine Teschovirus (PCR) Bluetongue (RT-PCR) Epizootic Hemorrhagic Disease (RT-PCR) African Horse Sickness (RT-PCR) Eastern Equine Encephalomyelitis (RT-PCR) Western Equine Encephalomyelitis (RT-PCR) West Nile Virus (RT-PCR) Equine Influenza (rrt-pcr) Virus Neutralization (VN) Vesicular Stomatitis Virus (New Jersey and Indiana Strains) Bovine Herpesvirus 1 (Infectious Bovine Rhintracheitis) Bovine Herpesvirus 2 (Bovine Herpes Mammillitis) Bovine Respiratory Syncytial Virus Bovine Viral Diarrhea Parainfluenza-3 Encephalomyocarditis Virus Malignant Catarrhal Fever Pseudorabies Virus Transmissible Gastroenteritis Virus Equine Herpesvirus 1 Equine Herpesvirus 2 Equine Herpesvirus 3 Equine Herpesvirus 4 Equine Viral Arteritis Bluetongue Epizootic Hemorrhagic Disease Plaque Reduction Neutralization Test (PRNT) West Nile Virus Eastern Equine Encephalomyelitis Western Equine Encephalomyelitis Venezuelan Equine Encephalomyelitis Hemagglutination/Hemagglutination-Inhibition (HA/HI) Avian Influenza (Virus Typing) Newcastle Disease (Virus Typing) Newcastle Disease (Serology) Avian Influenza (Serology) Swine Influenza Virus SOP-AV-0068, WI-AV-0039 SOP-AV-0068, WI-AV-0038 WI-AV-0035, WI-AV-0036, WI-AV-0037, WI-AV-0050 SOP-BPA-0018 SOP-BPA-0015 SOP-BPA-0016 SOP-BPA-0017 SOP-EO-0004 SOP-EO-0006 SOP-EO-0007 SOP-AV-0068, WI-EO-0030 SOP-BPA-0013 SOP-BPA-0032 SOP-BPA-0033 SOP-BPA-0034 SOP-BPA-0035, SOP-BPA-0039 SOP-BPA-0036 SOP-EO-0028 SOP-EO-0030 SOP-EO-0036 SOP-EO-0021 SOP-EO-0022 SOP-AV-0009 SOP-AV-0010 SOP-AV-0033 SOP-AV-0036 SOP-BPA-0027 (A2LA Cert. No ) 11/20/2017 Page 6 of 9
7 Hemagglutinating Encephalomyelitis Virus Eastern Equine Encephalomyelitis Western Equine Encephalomyelitis Venezuelan Equine Encephalomyelitis Equine Influenza Neuraminidase-Inhibition (NI) Avian Influenza (Virus Typing) Avian Influenza (Serology) Complement Fixation (CF) Vesicular Stomatitis Coxiella burnetti (Q-Fever) Chlamydophilia psittici Agar Gel Immunodiffusion (AGID) Avian Influenza Equine Infectious Anemia Bluetongue (VDT) Commercial Kit Bluetongue (VMRD) Commercial Kit Bovine Leukosis Virus (VDT) Commercial Kit Epizootic Hemorrhagic Disease (VDT) Commercial Kit Caprine Arthritis-Encephalitis Virus (VDT) Commercial Kit Ovine Progressive Pneumonia Virus (VDT) Commercial Kit SOP-BPA-0028 SOP-EO-0023 SOP-EO-0024 SOP-AV-0011 SOP-AV-0012 SOP-BPA-0010 SOP-EO-0018 SOP-EO-0035 SOP-AV-0045 SOP-EO-0034 SOP-EO-0017, REF-EO-0014 SOP-EO-0017, REF-EO-0015 REF-EO-0012 REF-EO-0022 REF-EO-0023 REF-EO-0023 Enzyme Linked Immunosorbent Assay (ELISA) Avian Metapneumovirus SOP-AV-0024 Vesicular Stomatitis (C-ELISA) SOP-BPA-0025 Porcine Reproductive and Respiratory Syndrome (IDEXX) Commercial Kit REF-BPA-0103 Pseudorabies Virus (gi ELISA) Commercial Kit SOP-BPA-0039, REF-BPA-0101 Pseudorabies Virus (gb ELISA) Commercial Kit SOP-BPA-0039, REF-BPA-0100 West Nile Virus (IgM ELISA) SOP-EO-0025 Eastern Equine Encephalomyelitis (IgM ELISA) SOP-EO-0026 Venezuelan Equine Encephalomyelitis (IgM ELISA) SOP-EO-0027 Equine Infectious Anemia (IDEXX, C-ELISA) Commercial Kit SOP-EO-0034, REF-EO-0002 Equine Infectious Anemia (Synbiotics, ViraCHEK) Commercial Kit SOP-EO-0034, REF-EO-0003 Equine Infectious Anemia (Centaur, Fusion Protein II) Commercial Kit SOP-EO-0034, REF-EO-0004 Equine Infectious Anemia (VMRD) Commercial Kit SOP-EO-0034, REF-EO-0029 Bluetongue (VDT) Commercial Kit REF-EO-0016 Bluetongue (VMRD) Commercial Kit REF-EO-0018 Bovine Leukosis Virus (VMRD) Commercial Kit REF-EO-0010 Caprine Arthritis-Encephalitis Virus (VMRD) Commercial Kit REF-EO-0020 Coxiella burnetti (Q-Fever) (IDEXX) Commercial Kit REF-EO-0024 Agglutination Pseudorabies Virus (Autolex) Commercial Kit In Vivo Intracerebral Pathogenicity Index Intravenous Pathogenicity Index Chicken Lethality Test SOP-BPA-0039, REF-BPA-0102 SOP-AV-0017 SOP-AV-0014 (A2LA Cert. No ) 11/20/2017 Page 7 of 9
8 Pathobiology Laboratory Agar Gel Immuno-double Diffusion (AGID) Species Verification ELISA Bovine Spongiform Encephalopathy Chronic Wasting Disease Scrapie Microscopic Examination Bovine Spongiform Encephalopathy Mycobacterium spp. CWD/Scrapie Hemoparasites Anaplasmosis Hemoparasites Dourine Hemoparasites Eperythrozoonosis Hemoparasites Bovine Piroplasmosis Hemoparasites Equine Piroplasmosis Identification Ectoparasites Fluke Ectoparasites Larvae Ectoparasites Lice Ectoparasites Maggots Ectoparasites Mange Ectoparasites Mites Ectoparasites Myiasis Ectoparasites Scabies Ectoparasites Screwworm Ectoparasites Tapeworm Histopathology Staining Auromine O and Acridine Orange Stain Congo Red Stain Giemsa Stain Gram Stain Grocott s Methanamine Silver Stain Hematoxylin and Eosin Stain Luxol Fast Blue Stain Modified Ziehl-Neelson Stain Periodic Acid-Schiff Stain Toluidine Blue O Stain Immunohistochemistry (IHC) Bovine Spongiform Encephalopathy Scrapie Chronic Wasting Disease SOP-CAS-0105 SOP-PPE-0031 SOP-PPE-0057 SOP-PPE-0059 SOP-PPE-0023 SOP-PPE-0014 SOP-PPE-0046 SOP-PPE-0012 SOP-PPE-0002, SOP-PPE-0003 SOP-PPE-0007 SOP-PPE-0002, SOP-PPE-0003, SOP-PPE-0007 SOP-PPE-0036 SOP-PPE-0049 SOP-PPE-0034 SOP-PPE-0041, SOP-PPE-0060 REF-PPE-0006 SOP-PPE-0037 SOP-PPE-0051 SOP-PPE-0035 SOP-PPE-0042 SOP-PPE-0050 SOP-PPE-0026 SOP-PPE-0046, WI-PPE-0060 PCR Mycobacterium spp. SOP-PPE-0043 (A2LA Cert. No ) 11/20/2017 Page 8 of 9
9 Western Blot Individual Animal Identification TeSeE Bio-Rad Kit (BSE, CWD, Scrapie) SOP-CAS-0109 SOP-PPE-0030 (A2LA Cert. No ) 11/20/2017 Page 9 of 9
10 Accredited Laboratory A2LA has accredited NATIONAL VETERINARY SERVICES LABORATORIES Ames, IA for technical competence in the field of Biological Testing This laboratory is accredited in accordance with the recognized International Standard ISO/IEC 17025:2005 General requirements for the competence of testing and calibration laboratories. This accreditation demonstrates technical competence for a defined scope and the operation of a laboratory quality management system (refer to joint ISO-ILAC-IAF Communiqué dated 8 January 2009). Presented this 20 th day of November President and CEO For the Accreditation Council Certificate Number Valid to June 30, 2019 For the tests to which this accreditation applies, please refer to the laboratory s Biological Scope of Accreditation.
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