Field study conducted in Tunisia to evaluate efficacy of an O-BFS vaccine
|
|
|
- Jeffrey Cook
- 5 years ago
- Views:
Transcription
1 Field study conducted in Tunisia to evaluate efficacy of an O-BFS vaccine FAO/OIE Reference Lab for FMD Emiliana Brocchi Institut de la Recherche Vétérinaire de Tunisie - Service Virologie Soufien Sghaier Istituto Zooprofilattico Sperimentale della Lombardia e dell'emilia-romagna Brescia - Italy
2 Vaccine matching for O/ME-SA/Ind-2001 Performed at WRL Pirbright Field isolate VACCINE STRAIN O-3039 O/TUR/5/09 O Manisa O BFS LIB 1/2013 0,51 >0,84 0,14 LIB 7/2013 >0,85 >0,86 0,16 LIB 17/2013 0,41 0,45 0,14 LIB 22/2013 >0,90 >1,0 0,50 O TUN/01/ (VN=182) 0.56 (91) 0.12 (64) 0.15 (72) O ALG/01/ (VN=190) 0.49 (82) 0.14 (72) 0.13 (57) NEP 18/2013 NEP 1/2014 SAU 1/2014 UAE 1/2014 UAE 2/2014 Key for results: MATCH (r 1 0.3), NO MATCH (r 1 <0.3), Borderline result ( ) In brackets neutralizing titres produced by heterologous vaccine strains 2 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
3 ObjecBve Predict efficacy of O- BFS vaccine in the Tunisian context METHOD: Evaluate the level of neutralizing anbbodies against the vaccine strain O- BFS and against the FMD circulabng virus in Tunisia (O Tunisia 2014) elicited by one vaccinabon with the O BFS vaccine. Concept: virus neutralizing anbbodies are able to inacbvate virus infecbvity and can be interpreted as esbmate of protecbon. 3 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
4 Experimental design and criteria Two groups of animals, not exposed to infection, selected for vaccination with O-BFS vaccine Naïve cattle and sheep: non-vaccinated, ideally without maternal antibodies Cattle and sheep previously vaccinated with the Tunisian vaccine (O Manisa + O Tunisa 1999) - five cattle out of 40 and 4 sheeps out of 40 kept as sentinels Sampling scheme Before vaccination (day 0) 5 days post vaccination (5 DPV) : evaluation of early immune-response to vaccine 21 days post-vaccination (21 DPV) : standard time to assess vaccine-induced immune response 4 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
5 AREAS SELECTED for the vaccination trial OTD MATEUR cattle & sheep prev. vaccinated Beja (cattle) and Medenine (sheep) non-vacc animals
6 MEDENINE LANDSCAPE SAMPLING
7 Mateur sheep 7 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
8 DR. SGHAIER SAMPLING SHEEP in MATEUR 8 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
9 9 PROF. HAMMAMI VACCINATING SHEEP in MATEUR JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
10
11 11 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
12 Testing scheme Parallel titration of sera by Virus Neutralization Test (VNT) against both: Ø the vaccine strain O-BFS (homologous titres) Ø the Tunisian circulating virus (heterologous titres) In addition to VNT, sera were analysed by ELISA kits for measurement of antibodies to NSP (marker of infection) and to serotype O. 12 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
13 PREVIOUSLY VACCINATED ANIMALS MEAN VNT TITRES (log2) N. 40 CATTLE (5 sentinels) - N. 40 SHEEP (4 sentinels) Threshold protecbon Threshold protecbon Both cattle and sheep showed low level of antibodies residual from previous vaccination (day 0) The vaccination with O-BFS vaccine elicited a strong and fast (5 DPV) increase of protecting antibodies against both type O strains Homologous titres (O-BFS) are only 2-fold higher than heterologous (field virus) Assuming a VN titre of log 2 6 (1/128) as indicative of protection, the results show that in the vaccinated population a booster vaccination with O-BFS elicits a protective immune-response as soon as 5 DPV 13 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
14 RESULTS OF INDIVIDUAL ANIMALS (vaccinated cattle) ODT Mateur Cattle (1-10) threshold +/ threshold protection log2 VN titre sentinel sentinel day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV ODT Mateur Cattle (11-20) log2 VN titre sentinel day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV 14 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
15 RESULTS OF INDIVIDUAL ANIMALS (vaccinated cattle) threshold +/ threshold protection ODT Mateur Cattle (21-30) log2 VN titre sentinel day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV ODT Mateur Cattle ( 31-40) log2 VN titre sentinel day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV 15 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
16 RESULTS OF INDIVIDUAL ANIMALS (vaccinated sheep) threshold +/ threshold protection ODT Mateur Sheep (1-10) log2 VN titre day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV ODT Mateur Sheep (11-20) log2 VN titre day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV 16 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
17 RESULTS OF INDIVIDUAL ANIMALS (vaccinated sheep) log2 VN titre sentinel ODT Mateur Sheep (21-30) sentinel threshold +/ threshold protection day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV sentinel ODT Mateur Sheep ( 31-40) sentinel log2 VN titre day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV 17 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
18 Other assays ELISA for type O antibodies : ü overall the results correlate and confirm those of VNT NSP-Abs (marker of infection): ü all seronegative at day 0 ü sero-conversion (low Ab level) in 5 cattle and 5 sheep at day 21 PV Hypothesis: ü immune-response to residual NSP in vaccine detectable only in few plurivaccinated animals ü circulation of FMD infection at low level few heads infected, no clinical signs (thanks to vaccine immunity) None of the NSP-seropositive animals corresponded to the sentinels, which might have been more susceptible to infection in the case of virus circulation 18 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
19 NAÏVE ANIMALS NON VACCINATED MEAN VNT TITRES 13 cattle and 21 sheep seronegative for SP and NSP Abs Threshold protecbon Threshold protecbon ü 21 days post-single vaccination cattle and sheep showed a significant sero-conversion ü Heterologous (O Tunisia 14) mean titres were 2-3 fold lower than homologous (O-BFS) ü Antibody levels achieved by this primary immune response were much lower (5-7 fold) than in boosted-vaccinated animals ü Nevertheless, 21 days after a single vaccination, the mean titres towards the field virus overcome the value log 2 7 (1/128), estimated to confer protection. 19 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
20 RESULTS OF INDIVIDUAL ANIMALS (non vaccinated CATTLE) M Beja Bovine (1-7) threshold +/ threshold protection log2 VN titre day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV M Beja Bovine (8-13) log2 VN titre day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV All cattle showed a sero-conversion, but 2 out of 13 (ie 15% cattle) did not reach a protective immunity against the circulating virus after a single vaccination with O-BFS vaccine 20 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
21 RESULTS OF INDIVIDUAL ANIMALS (non vaccinated SHEEP) Madeneine Sheep (1-10) threshold +/ threshold protection log2 VN titre day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV Madeneine Sheep (11-20) log2 VN titre day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV 21 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
22 RESULTS OF INDIVIDUAL ANIMALS (non vaccinated SHEEP) threshold +/ threshold protection Madeneine Sheep (21-29) log2 VN titre day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV day 0 5DPV 21DPV Similarly to cattle: ü all sheep showed sero-conversion at different levels 21 DPV ü all sheep reached protective immunity against the administered vaccine (strain O-BFS) ü but only 70% sheep reached the estimated protective titre against the circulating virus Therefore, one single vaccination with O-BFS is not expected to confer protection to all animals which will be vaccinated for the first time. 22 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
23 Other assays (non-vaccinated animals) ELISA for type O antibodies ü Overall the results correlate and confirm those of VNT NSP-Abs (marker of infection) ü All animals remained negative for anti-nsp antibodies for the duration of the experiment, proving absence of FMDV circulation 23 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
24 Direct comparison vaccinated vs naïve PREVIOUSLY VACCINATED SHEEP NAIVE Threshold protecbon Threshold protecbon ü the trend of immune response in the two groups is the same ü focalizing on Abs to the field virus, it is evident that the O-BFS mono-vaccinated sheep reached average titres about 3 log 2, i.e. 8-fold less, than the booster-vaccinated sheep ü 30% of sheep did not achieve a protective immunity against the field Tunisian virus 24 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
25 Direct comparison vaccinated vs naïve PREVIOUSLY VACCINATED CATTLE NAIVE CATTLE - OTD Mateur CATTLE - BEJA Mean VN titre (log2) day 0 5 DPV 21 DPV day 0 21 DPV Threshold protecbon threshold +/- ü MEAN titre of single-vaccinated animals overcome the estimated protecting level (log 2 = 7, 1/128) also for the field virus, BUT it is about 6-fold less than in the group of booster vaccinated CATTLE ü 15% of cattle did not achieve a protective immunity 25 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
26 OVERALL CONCLUSIONS The vaccination with O BFS vaccine is expected to induce a good immunity against the O Tunisia 2014 field virus in previously vaccinated animals (thanks to booster effect). However a proportion of naïve animals will not probably achieve a protective immunity with a single vaccination. The results observed at day 0 (before vaccination with O BFS) in the Mateur group (representing the situation of vaccinated farms) reflect the FMD immune status in the Tunisian context during the 2014 outbreak: approximately 50% cattle and 75% sheep showed neutralizing Ab titres against the field circulating virus lower than the estimated threshold of protection, thus indicative of a low protection level in the population. Also distance from last vaccination should be considered among factors that influence population immunity. 26 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
27 GENERAL CONCEPTS ü Protective immunity lasts up to 6 months after an initial course of vaccination, depending on the vaccine potency and the antigenic relationship between vaccine and outbreak strains ü An initial course of two vaccinations improves the strength and duration of the antibody response ü Longer lasting protection may develop once animals have received multiple vaccine doses 27 JPC REMESA Heraklion 16 th March 2015 Emiliana Brocchi
FMD in Tunisia Update situation Critical review Vaccination programme
FMD in Tunisia Update situation Critical review Vaccination programme Workshop on FMD vaccination strategy in North Africa Tunis, 30 31 March 2016 Direction Générale Des Services Vétérinaires Contents
FAO Collaborative Study Phase XVII: Standardisation of FMD Antibody Detection
Appendix 28 FAO Collaborative Study Phase XVII: Standardisation of FMD Antibody Detection D J Paton, R M Armstrong, L S Turner, P A Hamblin, M Corteyn, D Gibson, J Anderson Institute for Animal Health,
High potency vaccines induce protection against heterologous challenge with FMD virus
High potency vaccines induce protection against heterologous challenge with FMD virus Katharina Brehm, Naveen Kumar, Hans-Herman Thulke and Bernd Haas Principle of current production method for FMD vaccines
Foot and Mouth Disease Middle East situation Summary of Answers to the Questionnaire Beirut, Lebanon, 7 9 April 2009
Foot and Mouth Disease Middle East situation Summary of Answers to the Questionnaire Beirut, Lebanon, 7 9 April 2009 Dr Pierre Primot OIE for the Middle East Purpose of the Questionnaire The purpose of
Toward the control of Foot-and-Mouth disease in East Asia
Symposium on Prevention and Control of Foot and Mouth Disease and Highly Pathogenic Avian influenza in East Asia 20 September, 2017, Tokyo National Agriculture and Food Research Organization Toward the
Development of a predictive model for vaccine matching for serotype O FMDV from serology and capsid sequence
Development of a predictive model for vaccine matching for serotype O FMDV from serology and capsid sequence D. Borley, S. Upadhyaya, D. Paton, R. Reeve and Mana Mahapatra Pirbright Laboratory United Kingdom
Verification of Efficacy of Donated FMD O Type Vaccines in Laos
OIE Collaborating Centre Diagnosis and Control of Animal Diseases and Related Veterinary Product Assessment in Asia Activities of OIE/JFT Project in Laos September 25, 2014 Verification of Efficacy of
A solid-phase competition ELISA for measuring antibody to foot-and-mouth disease virus
197 Appendix 24 A solid-phase competition ELISA for measuring antibody to foot-and-mouth disease virus N.P. Ferris a, A.N. Bulut b, T. Rendle a, F. Davidson a and D.K.J. Mackay c a b c Institute for Animal
Vaccine matching. Reliability of foot-and-mouth disease r-values. determination
Vaccine matching Reliability of foot-and-mouth disease r-values determination Interinstitutional Network for R&D in FMD (RIIDFA)- Argentina Veterinary and Agrochemical Research Centre (VAR) - Belgium Protection
FMD Vaccine Strain Selection
FMD Vaccine Strain Selection David Paton, Pirbright, UK New Delhi, 13-15 February 2012 Conclusions Vaccine match is one component of vaccine efficacy Vaccine quality may compensate for imperfect match
External Quality Assurance: Subregional experiences on RVF
Regional Seminar for OIE National Focal Points for Veterinary Laboratories Towards a culture of safety and quality External Quality Assurance: Subregional experiences on RVF Gian Mario Cosseddu, Istituto
Longevity of the antibody response in pigs and sheep following a single administration of high potency emergency FMD vaccines
247 Appendix 31 Longevity of the antibody response in pigs and sheep following a single administration of high potency emergency FMD vaccines S. J Cox and P. V. Barnett Institute for Animal Health, Pirbright
Manufacturers expected contribution to the progressive control of Foot-and-Mouth Disease in South Asia
Manufacturers expected contribution to the progressive control of Foot-and-Mouth Disease in South Asia Ph. Dubourget & al. 1 GENERAL PRINCIPLES 2 EPIDEMIO- LOGICALLY RELEVANT STRAINS 3 IMMUNO- DOMINANT
16 th JPC REMESA meeting
16 th JPC REMESA meeting Morocco, 18-19 April 2018 EuFMD support programme to REMESA Pillar II Reduced FMD risk to Members from European neighbourhood UPDATE September 2017 March 2018 Targets across the
Appendix 30. Preliminary results to evaluate cross-protection between O 1 Manisa and O 1 Campos in cattle
Appendix 30 Preliminary results to evaluate cross-protection between O 1 Manisa and O 1 Campos in cattle V.A. Srinivasan 1, S.B.Nagendra Kumar 1, M.Madhan Mohan 1, V.Maroudam 1, P.Santha Kumar 1, S. Parida
COMPARISON OF THREE ELISA KITS FOR THE DETECTION OF ANTIBODIES AGAINST FOOT-AND-MOUTH DISEASE VIRUS NON-STRUCTURAL PROTEINS
Bull Vet Inst Pulawy 49, 147-151 COMPARISON OF THREE ELISA KITS FOR THE DETECTION OF ANTIBODIES AGAINST FOOT-AND-MOUTH DISEASE VIRUS NON-STRUCTURAL PROTEINS WIESŁAW NIEDBALSKI Department of Foot-and-Mouth
Longevity of protection in cattle following vaccination with emergency FMD vaccines from the UK strategic reserve
Longevity of protection in cattle following vaccination with emergency FMD vaccines from the UK strategic reserve Sarah Cox, Satya Parida, Pip Hamblin, Bartek Bankowski Veronica Carr, David Paton and Paul
Component 2.3 Support to REMESA
Appendi 11 88th Eecutive Committee meeting of the EuFMD Component 2.3 Support to REMESA Fabrizio Rosso 88ECom-Sofia, 13-14 October 2014 Component 2.3 Assist national FMD risk management as part of the
WEST EURASIA FMD LAB PHASE ACTIVITY PROPOSAL. A. Naci BULUT Head of the Diagnosis Department, Şap Institute
WEST EURASIA FMD LAB NETWORK WELNET FMD- 2ND PHASE ACTIVITY PROPOSAL FAO/the EuFMD Commission, 39th General Session, 27/28 April 2011 Rome, Italy A. Naci BULUT Head of the Diagnosis Department, Şap Institute
Simultaneous immunization of cattle with FMD and live anthrax vaccines
Simultaneous immunization of cattle with FMD and live anthrax vaccines Alejandra Capozzo Head of the Applied Veterinary Immunology Lab. Institute of Virology, Buenos Aires. Argentina In collaboration with:
Appendix 72 Using NSP ELISA (Chekit-FMD-3ABC Bommeli-Intervet) as a Tool for FMDV Serosurveillance in Bulgaria Abstract: Introduction
Appendix 72 Using NSP ELISA (Chekit-FMD-3ABC Bommeli-Intervet) as a Tool for FMDV Serosurveillance in Bulgaria Georgi Georgiev*¹, Emiliya Veleva¹, Liliyana Polihronova¹ and Alessandro Rossi² 1 National
Elements of FMD post-vaccination monitoring programme / Éléments du programme de surveillance post-vaccination contre la fièvre aphteuse
Workshop: harmonization of the FMD vaccination strategy in the North Africa / Atelier : harmonisation de la stratégie de vaccination contre la fièvre aphteuse en Afrique du Nord 30-31 mars 2016 - Tunis
DIFFERENTIATION OF INFECTION FROM VACCINATION BY DETECTION OF ANTIBODIES TO THE NON-STRUCTURAL PROTEIN 3ABC OF FOOT-AND-MOUTH DISEASE VIRUS
Bull. Vet. Inst. Pulawy 7, 5-6, DIFFERENTIATION OF INFECTION FROM VACCINATION BY DETECTION OF ANTIBODIES TO THE NON-STRUCTURAL PROTEIN ABC OF FOOT-AND-MOUTH DISEASE VIRUS WIESŁAW NIEDBALSKI AND BERND HAAS*
The New FMD Containment Zone Proposal and Vaccination. April 4, 2017, Pirenopolis, Brazil
The New FMD Containment Zone Proposal and Vaccination. April 4, 2017, Pirenopolis, Brazil Dr. Tom Smylie Senior Staff Veterinarian Canadian Food Inspection Agency 1 Topics for todays presentation. 1. Current
Results of Seromonitoring in FMD Buffer Zone in the CIS Countries
Appendix Results of Seromonitoring in FMD Buffer Zone in the CIS Countries T.A.Fomina, V.M.Zakharov, A.A.Gusev, N.E.Kamalova, S.R.Kremenchugskaya, O.A.Schekotova All-Russian Research Institute for Animal
Requirements of the Terrestrial Code for FMD surveillance. Dr David Paton Dr Gideon Brückner
Requirements of the Terrestrial Code for FMD surveillance Dr David Paton Dr Gideon Brückner 1 Surveillance Close observation Origin of the word: Early 19th century: from French, from sur- over + veiller
Global FMD Surveillance and Regional Risks for West Eurasia
Global FMD Surveillance and Regional Risks for West Eurasia Anna Ludi anna.ludi@pirbright.ac.uk WRLFMD Team: Donald King, Valerie Mioulet, Nick Knowles, Ginette Wilsden, Bryony Armson, Pip Hamblin, Kasia
The Global FMD situation: SEACFMD in the Global Context
The Global FMD situation: SEACFMD in the Global Context Anna Ludi anna.ludi@pirbright.ac.uk WRLFMD Team: Donald King, Valerie Mioulet, Nick Knowles, Anna Ludi, Ginette Wilsden, Bryony Armson, Pip Hamblin,
Mechanism of FMD spread in East Asia
Mechanism of FMD spread in East Asia China has the important role of the FMD outbreaks in East Asia 1) Long border with FMD epidemic countries in South East and Central Asia 2) Large numbers of susceptible
Cedivac-FMD; Duration of Immunity in cattle, sheep and pigs. 2004, 8203 AA Lelystad, The Netherlands * Corresponding Author
Appendix 3 Cedivac-FMD; Duration of Immunity in cattle, sheep and pigs. Paulus Selman *, Gilles Chénard and Aldo Dekker Animal Sciences Group, Wageningen UR, P.O. Box 65, 8 AB Lelystad, The Netherlands
FMD in Libya. Dr. Abdunaser Dayhum National Center of Animal Health Libya
FMD in Libya Dr. Abdunaser Dayhum National Center of Animal Health Libya Foot-and-Mouth Disease Cause: Foot-and-mouth disease virus (FMDV). A total of seven different serologic types are recognized: A,
FMD vaccination and postvaccination. guidelines. Samia Metwally (FAO) Animal Production and Health Division FAO of the United Nations Rome, Italy
4th Middle East FMD Roadmap Meeting Amman, Jordan 15-19 October 2017 FMD vaccination and postvaccination monitoring guidelines Samia Metwally (FAO) Animal Production and Health Division FAO of the United
IgA response of cattle to FMDV infection in probang and saliva samples
88 Appendix 9 IgA response of cattle to FMDV infection in probang and saliva samples Amadori M. 1, Haas B. 2, Moos A. 2 and Zerbini I. 1 1)Istituto Zooprofilattico Sperimentale della Lombardia e dell'emilia,
Mass vaccination, immunity and coverage: Modelling population protection against FMD in Turkish cattle. Theo Knight-Jones Epidemiologist, ILRI
Mass vaccination, immunity and coverage: Modelling population protection against FMD in Turkish cattle Theo Knight-Jones Epidemiologist, ILRI 1 Mass vaccination FMD vaccine protection: - Requires several
Foot-and Mouth Disease Ecological Studies In Endemic Settings: Ongoing Studies in Vietnam and Pakistan
Foot-and Mouth Disease Ecological Studies In Endemic Settings: Ongoing Studies in Vietnam and Pakistan Luis Rodriguez (Jonathan Arzt) Research leader, USDA-ARS Plum Island 1 PAKISTAN CHARACTERIZATION OF
Are Dromedary Camels Susceptible or Non-Susceptible to Foot-and-Mouth Disease Serotype O
Are Dromedary Camels Susceptible or Non-Susceptible to Foot-and-Mouth Disease Serotype O Soren Alexandersen Danish Institute for Food and Veterinary Research Department of Virology, Lindholm DK-4771 Kalvehave,
Lelystad, The Netherlands
Appendix 4 Emergency vaccination against FMD in pigs: influence of used vaccine dose and correlation between induced immunological responses and reduction of virus shedding P.L. Eblé 1 *, A. Bouma 2, K.
OIE RRL-Lanzhou Activities, Research and Lab network
Expanded 3 rd National Coordinators Meeting OIE/JTF project on FMD Control in east Asia, Sep 23-26 2014 Lanzhou, China OIE RRL-Lanzhou Activities, Research and Lab network Dr. Hong YIN OIE FMD Reference
Regional FMD threats to North Africa
Regional FMD threats to North Africa Donald King donald.king@pirbright.ac.uk WRLFMD Team: Valerie Mioulet, Nick Knowles, Anna Ludi, Gine>e Wilsden, Bryony Armson, Pip Hamblin, Kasia Bachanek-Bankowska,
Post-Vaccination Monitoring
Post-Vaccination Monitoring Samia Metwally, DVM, PhD Senior Animal Health Officer (Virologist) Animal Production and Health Division FAO of UN, Rome, Italy samia.metwally@fao.org Overview Why post-vaccination
Validation report. First commercially-available ELISA which allows for the detection of Lumpy Skin Disease antibodies
Validation report ID Screen Capripox Double Antigen Multi-species Multi-species Double Antigen ELISA for detection of antibodies against capripoxviruses including Lumpy skin disease virus (LSDV), sheeppox
Global Update EuFMD Exec. Committee
Global Update EuFMD Exec. Committee Donald King donald.king@pirbright.ac.uk WRLFMD Team: Valerie Mioulet, Nick Knowles, Anna Ludi, Ginette Wilsden, Bryony Armson, Pip Hamblin, Kasia Bachanek-Bankowska,
Update from WRLFMD. Donald King
Update from WRLFMD Donald King donald.king@pirbright.ac.uk WRLFMD Team: Valerie Mioulet, Nick Knowles, Anna Ludi, Ginette Wilsden, Bryony Armson, Pip Hamblin, Kasia Bachanek-Bankowska, Lissie Henry, Antonello
FMD Carrier state and role of carrier buffalo as source of transboundary spread in Southeast Asia and Eastern Asia Satya Parida
FMD Carrier state and role of carrier buffalo as source of transboundary spread in Southeast Asia and Eastern Asia Satya Parida, Pirbright, UK Foot-and-mouth disease Highly contagious disease of all the
FMD regional update Gulf States 2017
FMD regional update Gulf States 2017 Nick Lyons on behalf of Donald King donald.king@pirbright.ac.uk WRLFMD Team: Valerie Mioulet, Nick Knowles, Anna Ludi, Ginette Wilsden, Bryony Armson, Pip Hamblin,
Testing the Antibody Response of Pigs to Foot-and- Mouth Disease Vaccines
Testing the Antibody Response of Pigs to Foot-and- Mouth Disease Vaccines Final Report APL Project 211/139.45 December 211 Australian Animal Health Laboratory Dr Wilna Vosloo Private Bag 24 Geelong Vic
FMD Control in Dairy Colonies Milk Production System in Pakistan
FMD Control in Dairy Colonies Milk Production System in Pakistan M. Afzal, Manzoor Hussain, Ehtisham Khan & Giancarlo Ferrari 1 FAO Project GCP/PAK/123/USA, Islamabad, Pakistan; 1 Project Coordinator (GTFS/INT/907/ITA),Rome,
Exploiting serological data to understand the epidemiology of foot-and-mouth disease virus serotypes circulating in Libya
Open Veterinary Journal, (2017), Vol. 7(1): 1-11 ISSN: 2226-4485 (Print) ISSN: 2218-6050 (Online) Original Article DOI: http://dx.doi.org/10.4314/ovj.v7i1.1 Submitted: 25/07/2016 Accepted: 30/12/2016 Published:
Open to: Model developers and users with an interest in the objective
Meeting of the Modeling Network: 15:30 on Thursday, parallel session Network objective: promote a better understanding of existing decision support tools for contingency planning, Improve dialog and awareness
FMD - TURKEY. Yener ŞEKERCAN. Veterinary Officer General Directorate of Food and Control Ankara/TURKEY
FMD - TURKEY Yener ŞEKERCAN Veterinary Officer General Directorate of Food and Control Ankara/TURKEY 4th Middle East FMD Roadmap Meeting Amman, Jordan 15-19 October 2017 4th Middle East FMD Roadmap Meeting
Republic of Kazakhstan
Republic of Kazakhstan Samat Tyulegenov OIE Delegate Status of the Republic of Kazakhstan on FMD On the 83d OIE General Session in 2015 the Republic of Kazakhstan was officially recognised as zone FMD
Syrian Arab Republic. ministry of agriculture and agrarian reform- directorate of animal health- Syria
Syrian Arab Republic ministry of agriculture and agrarian reform- directorate of animal health- Syria FMD incidence The Directorate of Animal Health conducts annually many surveys but there are no cases
Vaccine Matching and Development of Appropriate Vaccines
Vaccine Matching and Development of Appropriate Vaccines Dr Gaolathe Thobokwe SSARRL @Botswana Vaccine InsBtute Reference Laboratories and Collabora-ng Centres Addi-onal Reference Centres Regional/Na-onal
PROGRESS REPORT FOR TURKEY ON FMD SITUATION AND CONTROL MEASURES
Appendix 4 PROGRESS REPORT FOR TURKEY ON FMD SITUATION AND CONTROL MEASURES Dr H Askaroglu Introduction Foot-and-Mouth Disease (FMD) is endemic in the Anatolia Region of Turkey, due to two serotypes. Types
COMPARISON OF DIFFERENT ELISA METHODS FOR THE DETECTION OF ANTIBODIES AGAINST FOOT-AND-MOUTH DISEASE VIRUS (FMDV) TYPE O
Bull. Vet. Inst. Pulawy 48, 5-9, 24 COMPARISON OF DIFFERENT ELISA METHODS FOR THE DETECTION OF ANTIBODIES AGAINST FOOT-AND-MOUTH DISEASE VIRUS (FMDV) TYPE O WIESŁAW NIEDBALSKI Department of Foot-and-Mouth
FMD VACCINE AND VACCINATION. Ahmad Al-Majali Dean, Faculty of Vet Medicine JUST Jordan
FMD VACCINE AND VACCINATION Ahmad Al-Majali Dean, Faculty of Vet Medicine JUST Jordan General Considerations on FMD vaccination The currently used FMD vaccines are killed virus preparations that are pure,
Global Update EuFMD Exec. Committee
Appendix 2 Global Update EuFMD Exec. Committee Donald King donald.king@pirbright.ac.uk WRLFMD Team: Valerie Mioulet, Nick Knowles, Anna Ludi, Ginette Wilsden, Bryony Armson, Pip Hamblin, Kasia Bachanek-Bankowska,
Findings on FMD in Afghanistan and Pakistan
Findings on FMD in Afghanistan and Pakistan Giancarlo Ferrari Project Coordinator GTFS/INT/907/ITA Controlling Trans-boundary Animal Diseases in Central Asian countries AGAH/EMPRES FAO Outline of the presentation
Strengthening FMD prevention and emergency response capacity in the Trans-Caucasian countries (MTF/INT/003/EEC)
Progress towards long term FMD control in the Trans-Caucasus Strengthening FMD prevention and emergency response capacity in the Trans-Caucasian countries (MTF/INT/003/EEC) Carsten Pötzsch, EuFMD/FAO K.Sumption
Materials and Methods
Appendix 18 Repeated administration of maximum payload emergency vaccines made from inactivated purified antigen concentrates do not induce significant titres of antibodies against non-structural proteins
Dr Ghazi Yehia OIE Regional Representative for the Middle East
Foot and mouth disease control strategies in North Africa and the Middle East The current situation Asuncion, Paraguay, 24-26 June 2009 Dr Ghazi Yehia OIE Regional Representative for the Middle East Acknowledgements
Importance of cell mediated immunity for protection against Foot and Mouth Disease
Appendix 34 Importance of cell mediated immunity for protection against Foot and Mouth Disease Yooni Oh 1*, ryan Charleston 1, David J. Paton 1, Jong-Hyun Park 2, Paul V. arnett 1, Yi-Seok Joo 2, Satya
Direct beneficiary Countries: Georgia, Armenia and Azerbaijan Proposed Implementation date: 1/1/06
Appendix 9 Outline of Project Proposal: Co-ordination of FMD surveillance and control in the Trans-Caucasus countries and strengthening of emergency management capacity Direct beneficiary Countries: Georgia,
Experimental evaluation of foot-and-mouth disease vaccines for emergency use in ruminants and pigs: a review
Vet. Res. (2009) 40:13 DOI: 10.1051/vetres:2008051 C INRA, EDP Sciences, 2009 www.vetres.org Review article Experimental evaluation of foot-and-mouth disease vaccines for emergency use in ruminants and
SEROLOGICAL EVALUATION OF THE IMMUNITY INDUCED IN COMMERCIAL RABBITS BY VACCINATION FOR MYXOMATOSIS AND RHD
SEROLOGICAL EVALUATION OF THE IMMUNITY INDUCED IN COMMERCIAL RABBITS BY VACCINATION FOR MYXOMATOSIS AND RHD LAVAZZA A. 1,2, GRAZIANI M. 3, TRANQUILLO V.M. 1, BOTTI G. 1,2, PALOTTA C. 1, CERIOLI M. 1, CAPUCCI
THE CYTOPATHOGENIC ACTION OF BLUETONGUE VIRUS ON TISSUE CULTURES AND ITS APPLICATION TO THE DETECTION OF ANTIBODIES IN THE SERUM OF SHEEP.
Onderstepoort Journal of Veterinary Research, Volume 27, Number 2, October, 1956. The Government Printer. THE CYTOPATHOGENIC ACTION OF BLUETONGUE VIRUS ON TISSUE CULTURES AND ITS APPLICATION TO THE DETECTION
1. Engineering Foot-and-Mouth Disease Viruses with Improved
Engineering Foot-and-Mouth Disease Virus with Improved Properties for the Development of Effective Vaccine Haixue Zheng, Fan Yang, Ye Jin, Jianhong Guo, Jijun He, Lvlv, Xuepeng Cai, Xiangtao Liu, Hong
Paper on needs and support to be provided to North African countries, members of REMESA regarding the coordinated control of FMD
Appendix 4 40 th EuFMD General Session, 22 24 April 2013, Rome Paper on needs and support to be provided to North African countries, members of REMESA regarding the coordinated control of FMD Prepared
PPR in Libya. Zakaria Alkhatal. Abdunaser Dayhum and Ibrahim Eldaghayes. Scientific Advisors (NCAH) Libyan CVO, Director General (NCAH)
PPR in Libya Zakaria Alkhatal Libyan CVO, Director General (NCAH) Abdunaser Dayhum and Ibrahim Eldaghayes Scientific Advisors (NCAH) 4//07 5th JPC/REMESA, Jordan, 0- November 07 Libya 4//07 5th JPC/REMESA,
FMD activities in the North African region since the 2014/2015 epidemic
ALESSANDRO RIPANI Programme officer, OIE Sub-Regional Representation for North Africa FMD activities in the North African region since the 2014/2015 epidemic 12 th JPC REMESA Toledo, Spain 10-11 May 2016
Key words: foot-and-mouth disease; single emulsion vaccine, saponin, immune response, emergency vaccines.
255 Appendix 32 Enhancement of the immune response induced by the inclusion of saponin in oil adjuvanted vaccines against foot-and-mouth disease E. Smitsaart (1), N. Mattion (1) ; J.L. Filippi (1) ; B.
Appendix 68 3 ABC ELISA for the diagnosis of FMD in Egyptian sheep Abstract Introduction
Appendix 68 3 ABC ELISA for the diagnosis of FMD in Egyptian sheep Laila E. El-Shehawy, Samira El-Kilany & A.M. Doaud Foot and Mouth Disease Department, Veterinary Serum and Vaccine Research Institute,
Regional Status and FMD s Control Strategies in North Africa
Regional Status and FMD s Control Strategies in North Africa Jaouad BERRADA, DVM, Ph.D, Director of Veterinary Services National Office of Sanitary Food Safety Rabat-Morocco SUMMARY - Introduction - Importance
The global control of FMD; challenges and opportunities
EuFMD-Erice 2008 The global control of FMD; challenges and opportunities Keith Sumption, Secretary, European Commission for the Control of Foot-and-Mouth Disease (EuFMD), Food-and-Agriculture Organization
Engineering Foot-and-Mouth Disease Virus with Improved Properties for the Development of Effective Vaccine Introduction: Materials and methods:
Engineering Foot-and-Mouth Disease Virus with Improved Properties for the Development of Effective Vaccine Haixue Zheng, Fan Yang, Ye Jin, Jianhong Guo, Jijun He, Kaiqi Lian, Zixiang Zhu, Weijun Cao, Lvlv,
Excretion of bluetongue virus in cattle semen: a feature of laboratory-adapted virus
Vet. Ital., 40 (4), 497-501 Bluetongue virus and disease Excretion of bluetongue virus in cattle semen: a feature of laboratory-adapted virus P.D. Kirkland (1), L.F. Melville (2), N.T. Hunt (2), C.F. Williams
FMD diagnostics: current developments and application in the context of FMD control in endemic countries Wilna Vosloo
FMD diagnostics: current developments and application in the context of FMD control in endemic countries Wilna Vosloo CSIRO Livestock Industries, Australian Animal Health Laboratory (AAHL), Geelong, Australia
FMD Report - Syria 6 th Regional FMD West Eurasia Roadmap Meeting - Almaty, Kazakhstan 28 to 30 April 2015
FMD Report - Syria 6 th Regional FMD West Eurasia Roadmap Meeting - Almaty, Kazakhstan 28 to 30 April 2015 Dr. Mazen Dib - Directorate Of Animal Health Syria 6th West Eurasia Roadmap Meeting Almaty, Kazakhstan
Min Levine, Ph. D. Influenza Division US Centers for Disease Control and Prevention. June 18, 2015 NIBSC
Workshop on Immunoassay Standardization for Universal Flu Vaccines Min Levine, Ph. D. Influenza Division US Centers for Disease Control and Prevention June 18, 2015 NIBSC 1 Multiple Immune Mechanisms Contribute
Progressive Control of Foot and Mouth Disease in PAKISTAN
Progressive Control of Foot and Mouth Disease in PAKISTAN Muhammad Afzal 1, Manzoor Hussain 2 and R. H. Usmani 3 1 Project Coordinator, 2 National Project Director, GCP/PAK/123/USA, FAO Pakistan Office,
EFSA projects on PPR, sheep pox, lumpy skin disease. Franck Berthe Animal and Plant Health Unit European Food Safety Authority - EFSA
EFSA projects on PPR, sheep pox, lumpy skin disease Franck Berthe Animal and Plant Health Unit European Food Safety Authority - EFSA 9th JPC REMESA Tunis, Tunisie - 03 and 04 November 2014 BACKGROUND PPR,
landbouw, natuur en voedselkwaliteit Epidemiological report BTV 6 in the Netherlands
landbouw, natuur en voedselkwaliteit Epidemiological report BTV 6 in the Netherlands The Hague, 4 March 2009 Content 1. Introduction 2. Tracing of a possible introduction route 2.1 First infection in the
Predicting antigenic sites on the FMDV. F.F. Maree, R. Reeve, B. Blignaut, J.J. Esterhuysen, E. Fry, T. de Beer, E. Rieder and D.
Predicting antigenic sites on the FMDV capsid from cross-reactivity reactivity data F.F. Maree, R. Reeve, B. Blignaut, J.J. Esterhuysen, E. Fry, T. de Beer, E. Rieder and D.Haydon Introduction The SAT
O 38% NVD 42% A 10% Asia 1 9% SAT2 1% SAT1 <1% Institute for Animal Health
Serotypes Identified 2011 NVD 42% 38% Asia 1 9% A 10% Institute for Animal Health 1% SAT1 600 positive >900 Samples from 23 Countries 23 countries ~900 samples Bulgaria Turkey, A
Akabane Virus Risk Management in Australia. P.D. Kirkland, EMAI, Camden NSW Australia.
Akabane Virus Risk Management in Australia P.D. Kirkland, EMAI, Camden NSW Australia. Akabane virus epidemiology Understanding the epidemiology of Akabane virus is the key to: Disease control Risk management
Foot and Mouth Disease Control, A vaccine perspective. John Barlow, DVM PhD
Foot and Mouth Disease Control, A vaccine perspective John Barlow, DVM PhD john.barlow@uvm.edu May 28, 2015 Countries in which FMD was reported to the OIE between 1990 and 2002 Antigenic variation is common
This CRP is proposed for five years with three RCM. To apply, please see our website for directions:
1. CRP on the control of foot-and-mouth disease 2. Summary Foot-and-mouth disease (FMD) is one of the most important livestock diseases known to man due to its high infection rate (ease of spread) and
Importance of Vaccines for Managing FMD
Importance of Vaccines for Managing FMD WILDLIFE-FRIENDLY BEEF: WORKING TOWARDS A WIN-WIN SOLUTION FOR LIVESTOCK AGRICULTURE & WILDLIFE CONSERVATION IN NGAMILAND Maun, Botswana 8-9 November 2017 CONSIDERATIONS
Progress on Implementation of Global FMD Control
Progress on Implementation of Global FMD Control Part II. Joseph Domenech OIE,Paris 41 th General Session of the European Commission for the control of Foot and Mouth Disease (EuFMD) 23-24 th April 2015,
REGIONAL REFERENCE LABORATORY FOR FMD IN SOUTH EAST ASIA DEPARTMENT OF LIVESTOCK DEVELOPMENT PAKCHONG, NAKHONRATCHASIMA, THAILAND
University of Tokyo, Tokyo, Japan, 9-11 June 2015 REGIONAL REFERENCE LABORATORY FOR FMD IN SOUTH EAST ASIA DEPARTMENT OF LIVESTOCK DEVELOPMENT PAKCHONG, NAKHONRATCHASIMA, THAILAND Activities of FMD Laboratory
Regulation of FMD vaccines within the European Union
Introduction Regulation of FMD vaccines within the European Union K De Clercq 1 and D K J Mackay 2 Appendix 36 The EUFMD European Pharmacopoeia Working Group made a proposal for revision of the FMD vaccine
Foot and Mouth Disease vaccination is effective: Quantification of FMD transmission with and without vaccination
Foot and Mouth Disease vaccination is effective: Quantification of FMD transmission with and without vaccination Aldo Dekker Acknowledgments Phaedra Eblé, Karin Orsel, Carla Bravo de Rueda Annemarie Bouma,
The Global control of FMD - Tools, ideas and ideals Erice, Italy October 2008
Appendix 14 FOOT-AND-MOUTH DISEASE (FMD) VACCINATION STRATEGIES IN SHEEP AND LAMBS BY USING COMMERCIAL OIL VACCINE E.J.A. Späth 1, B. Robiolo 2, E.A. León 3, J.A. Manazza 1, S.J. Duffy 3, J. Filippi 4,
INTERNATIONAL WORKSHOP BLUETONGUE VACCINES 19 February 2013, Strasbourg, France ABSTRACTS
INTERNATIONAL WORKSHOP BLUETONGUE VACCINES 19 February 2013, Strasbourg, France ABSTRACTS ASSESSMENT OF THE EFFICACY OF BLUE TONGUE VACCINES: SYVA S EXPERIENCE Dr María Luisa DE ARRIBA Laboratorios Syva,
Vet. Ital., 40 (4), islands
Vet. Ital., 40 (4), 611-615 Vaccines Bluetongue control using vaccines: experience of the Mediterranean islands G. Gerbier (1), P. Hendrikx (2), F. Roger (1), S. Zientara (3), F. Biteau-Coroller (1), C.
Situtation of France regarding BTV 8. 8th october 2015 A. Fediaevsky, MAAAF/DGAL/SDSPA/BSA
Situtation of France regarding BTV 8 8th october 2015 A. Fediaevsky, MAAAF/DGAL/SDSPA/BSA French situation in August 2015 Official statut Mainland France is free of BTV since december 2012 Corsica under
EPIDEMIOLOGICAL DEVELOPMENTS AND CONTROL OF FOOT AND MOUTH DISEASE IN ASIA
EPIDEMIOLOGICAL DEVELOPMENTS AND CONTROL OF FOOT AND MOUTH DISEASE IN ASIA Kenichi Sakamoto National Institute of Animal Health, Japan 1 Original: English Summary: In the Asia region, foot and mouth disease
92th Executive committee
92th Executive committee Workplan 2015-2017 Some pictures from activities (Period March-Sept 2017) Workplan 2015-2017 OBJECTIVE Assist national FMD risk management as part of the REMESA action plan Help
Global FMD Surveillance Report
Global FMD Surveillance Report Donald King WRLFMD donald.king@pirbright.ac.uk WRLFMD Team: Valerie Mioulet, Nick Knowles, Anna Ludi, Ginette Wilsden, Bryony Armson, Pip Hamblin, Kasia Bachanek-Bankowska,
Global Update. EuFMD Exec. Committee. Donald King
Global Update EuFMD Exec. Committee Donald King donald.king@pirbright.ac.uk WRLFMD Team: Valerie Mioulet, Nick Knowles, Anna Ludi, Ginette Wilsden, Bryony Armson, Pip Hamblin, Kasia Bachanek-Bankowska,