OIE RRL-Lanzhou Activities, Research and Lab network
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1 Expanded 3 rd National Coordinators Meeting OIE/JTF project on FMD Control in east Asia, Sep Lanzhou, China OIE RRL-Lanzhou Activities, Research and Lab network Dr. Hong YIN OIE FMD Reference Laboratory. Lanzhou Veterinary Research Institute, CAAS;
2 Outline Re-organizing in the laboratory FMD outbreak confirmation and strains analysis Laboratory activities and network Active surveillance FMD research progress in LVRI
3 Outline Re-organizing in the laboratory FMD outbreak confirmation and strains analysis Laboratory activities and network Active surveillance FMD research progress in LVRI
4 The Agricultural Science and Technology Innovation Program (ASTIP), 2013, financially supported by central government Each year 5 million YUAN (RMB) FMD Etiology and Epidemiology Team FMDV Biology Team FMD Prevention and Control Team FMD Diagnostic Reagent Producing Center
5 FMD Etiology and Epidemiology Team Outbreak confirmation of FMD FMD surveillance and epidemiology; Identification and characterization of FMDV; Etiology variations, phylogenetic analysis and molecular evolution; Chief scientist Dr. Liu Xiangtao FMD outbreaks and types in China
6 FMDV Biology Team FMDV characterization FMDV pathogenesis, FMDV ecology, FMDV immune mechanism; FMDV reverse genetics Chief scientist Dr. Liu aixin FMDV genomics, transcriptomics and proteinomics
7 FMD Prevention and Control Team Study on conventional vaccine; Novel FMD molecular vaccines; Key techniques for vaccine production Penside diagnosis kits Chief scientist Dr. Zhang Yongguang High throughtput diagnostic tools
8 FMD Diagnostic Reagent Producing Center Chief scientist Dr Lin Mi Producing the FMD diagnostic kits Improving the producing technique Commercializing the kit
9 Outline Re-organizing in the laboratory FMD outbreak confirmation and strains analysis Laboratory activities and network Active surveillance FMD research progress in LVRI
10 1. Summary of FMD situation in China during serotype: A and O 3 Strains: A/Sea-97; O/Mya-98; O/PanAsia No. of outbreaks: 2013:n=23 (A17+O6) 2014:n=3 (A2+O1) Animals affected: Cattle; Pig Provinces occured: A: 6 provinces O/Mya-98: 3 O/PanAsia: 1 Effective control Trending downwards
11 FMDV Type A FMDV Type O 2014 Xinjiang n=4 Tibet n=10+1 Qinghai n=1 Jiangsu n= Pig Yunnan n=1 Guangdong n=1 Type O Apr 18, 2014 Jiangxi, Yintan cattle
12 MOLECULAR EPIDEMIOLOGY ANALYSIS A/Sea-97 strain located on the other genetic branch(named G2) low genetic relationship with the virus(named G1) found in China in 2009: about 91% identity new virus found in China again Shared close relationship with viruses from SEA nations past two years: over 98% homology also very close relationship with the sequences from RUS, KAZ, MOG
13 Analysis on O/Mya-98 strains Firstly found in 2010 in China; Came from SEA nations ; One of predominant strains in China; Total 26 cases reported; Also found in East Asia; Outbreaks are on the decline; Vaccines used in China are effective for the strains.
14 Outline Re-organizing in the laboratory FMD outbreak confirmation and strains analysis Laboratory activities and network Active surveillance FMD research progress in LVRI
15 Diagnosis and Reagents provided in 2014, Jan-Jul 31 suspected samples detected, 5 from North Korea ~3000 O/P fluid and tissue samples were tested by RT- PCR for routine survey and emergency. ~20 Vp1 sequences for field strain or isolates. ~8,000 serum tested by LPBE and 3ABC ELISA for SP and NSP antibody Type of reagent Guinea pig antisera (against FMDV type O,Asia1 and A) Related diagnostic test VNT Amount supplied nationally 20ml FMD NSP positive serum ELISA 124ml FMD NSP negative serum ELISA 31ml FMD LBP-ELISA kit(type O,A,Asia1) FMDV 3ABC-I-ELISA kit IHA(type O) FMD antibody Colloid-gold test strips FMDV mutiple RT-PCR kits ELISA for FMDV 6024 kits Antibody detection ELISA for FMDV NSP Antibody 1016kits detection IHA for FMDV 5226 kits Antibody detection pen-side test for FMDV Antibody 27 kits detection RT-PCR for FMDV molecular detection 151 kits
16 Network and training Diagnosis technology prevention and control technology Vaccination technique Theory teaching Laboratory skill training Provincial laboratory Breeding enterprise technical staff Visitors ~15 special training courses ~300 people attended
17 In east Asia countries Samples from DPR Korea Training of colleagues from Mongolia, financially supported by IAEA Visiting of colleagues from RO Korea Providing diagnostic reagents to DPR Korea
18 Outline Re-organizing in the laboratory FMD outbreak confirmation and strains analysis Laboratory activities and network Active surveillance FMD research progress in LVRI
19 Active surveillance work plan in ) FMD surveillance on pig slaughterhouses in 12 provinces in China 2) FMD surveillance around Jiaodong peninsula and neighbour provinces in China 3)Type Asia1 FMD immune policy ealuation monitoring 4) Monitoring in high-risk areas (Guangxi, Yunnan) 5)Surveillance on FMD free zone with vaccination in China Laboratory testing is in progress
20 Outline Re-organizing in the laboratory FMD outbreak confirmation and strains analysis Laboratory activities and network Active surveillance FMD research progress in LVRI
21 vaccine development and registration Type O-A-Asia1 trivalent vaccine Synthetic Peptide Vaccine (Type O, pigs only)
22 New vaccine for type A Re-A WH/09 Gene modify vaccine based reverse genetics system FMD type A Vaccine potency challenged with A/GDMM/2013 strain PGP test: Animal test (protection Rate) AF72 Re-A WH/09 A/GDMM/13 13/16 (>75%) 15/16 (>75%) 14/16 (>75%)
23 Recombinant virus marker vaccine (serotype O,A,Asia1) O/Mya/98 FMDV 639nts VP Deletion can differ vaccinated and infected animals 3A deletion in native virus Transfection Rescue of virus Dr. Zheng haixue Dr. Liu zaixin
24 Virus-like particles vaccine (serotype Asia1,O) Dr. Sun shiqi Vaccination with a single injection of 50µg of proteins could elicit a high level of immune response, which is sufficient to protect guinea pigs, swine and cattle from virulent virus infection.
25 Peptide vaccine (serotype A, Asia 1) Fig. 1 Antibody level of guinea pigs post vaccination Fig. 2 Neutralizing antibody titres of guinea pigs Table1:Evaluation of clinical signs of FMD in guinea pigs challenged with FMDV A/HB/WH09
26 Multi-epitope vaccine(serotype O) Epitope protein vaccine contains neutralization epitope and general T cell epitope from three serotype O topological virus Cellular immune response in pigs Dr. Chang huiyun changhuiyun@caas.cn
27 Thank you for your attention
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