Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Department of Clinical Chemistry Laboratory Medicine Centre Northern General Hospital Herries Road Sheffield S5 7U United Kingdom Contact: Kevin Green Tel: +44 (0) Website: Laboratory locations: below s covered by the organisation and their relevant activities details ctivity code ddress Department of Clinical Chemistry Laboratory Medicine Centre (North Lane) Northern General Hospital Herries Road Sheffield S5 7U Local contact Kevin Green Clinical iochemistry ddress Department of Clinical Chemistry Royal Hallamshire Hospital Glossop Road Sheffield S10 2JF Local contact Kevin Green Clinical iochemistry ssessment Manager: MH Page 1 of 13

2 DETIL OF CCREDITTION HUMN ODY FLUIDS iochemistry examination clinical diagnosis Serum lbumin Roche Cobas 8000 lkaline Phosphatase, LT SOP CCTUTO045 ST mylase mmonia icarbonate ilirubin Direct bilirubin Calcium Cholesterol Creatinine Kinase Creatinine (Jaffe) Glucose Protein Iron Unsaturated Iron inding Capacity LDH Triglyceride Urea Gamma-GT HDL Cholesterol Lithium Phosphate Magnesium Uric cid CSF Protein Glucose Lactate Plasma Lactate Enzyme immunoassay Serum Vancomycin Enzyme immunoassay ssessment Manager: MH Page 2 of 13

3 HUMN ODY FLUIDS iochemistry examination clinical diagnosis Roche Cobas 8000 using SOP CCTUTO045 Serum C-reactive protein Particle enhanced immunoturbidimetric assay, Serum, Urine Sodium Indirect ion selective electrode, Potassium Chloride Serum Creatinine (Enzymatic) Gentamicin Cloned enzyme donor immunoassay Paracetamol Salicylate Ethanol eta-hydroxbutyrate Tobramycin Enzyme immunoassay Valproate Enzyme immunoassay, Carbamazepine Phenobarbitone ile cids ngiotensin Converting Enzyme Kinetic interaction of microparticles in solution assay Kinetic interaction of microparticles in solution assay Roche Cobas 8000 using Diazyme reagents RCCUTO103 Roche Cobas 8000 using uhlmann reagents RCCUTO103,, ssessment Manager: MH Page 3 of 13

4 HUMN ODY FLUIDS iochemistry examination clinical diagnosis Roche Cobas 8000 SOP CCTUTO045 Phenytoin Kinetic interaction of microparticles in solution assay, Theophylline Kinetic interaction of microparticles in solution assay, Urine Microalbumin Immunoturbidimetric assay, Protein mylase Calcium Creatinine (Jaffe) Glucose Urea Phosphate Magnesium Uric cid ssessment Manager: MH Page 4 of 13

5 HUMN ODY FLUIDS iochemistry examination clinical diagnosis Chemiluminescent immunoassay Roche Cobas 8000 SOP CTTUTO045 Serum Ferritin, 12 eta hcg Cortisol Free T4 Free T3 TSH Digoxin high sensitivity Troponin T Serum Vitamin D Protein binding assay Folate Protein binding assay Plasma Parathyroid hormone Chemiluminescent immunoassay Serum FSH Chemiluminescent immunoassay LH Oestradiol Testosterone ssessment Manager: MH Page 5 of 13

6 HUMN ODY FLUIDS iochemistry examination clinical diagnosis Chemiluminescent immunoassay Roche Cobas 8000 SOP CTTUTO045 Serum Progesterone SHG Prolactin Insulin nti-mullerian hormone Growth hormone P1NP nt-pronp Teicoplanin Thermo reagent, QMS immunoassay Urine Drugs of abuse screening: Roche reagents c501 Immunoturbidimetric assay mphetamine RCCTX7069 arbiturate enzodiazepine Cannabinoids Cocaine Methadone Opiates uprenorphine ssessment Manager: MH Page 6 of 13

7 HUMN ODY FLUIDS iochemistry examination clinical diagnosis Urine Drugs of abuse confirmation assay for above drugs In house assay, high resolution mass spectrometry using Thermo Fisher Q Exactive LCMS and SOP RCCTX 0084 In house assay, GCMS using gilent 5575 and RCCTX0003, 0016 lood, Urine Ethanol In house assay using Varian Head Space GC and SOP RCCTX0008 Methanol lood Ethylene glycol In house assay using gilent 5575 GC and SOP RCCTX0042 ssessment Manager: MH Page 7 of 13

8 HUMN ODY FLUIDS iochemistry examination clinical diagnosis Serum, blood, gastric contents, urine Lamotrigine Methadone In house assay using Thermo Fisher ccela HPLC and SOP S RCCTX0048 Diazepam RCCTX0011 Nordiazepam RCCTX0011 Temazepam RCCTX0011 mitriptyline Nortriptyline Dothiepin Nordothiepin Imipramine Desipramne Fluoxetine Trazodone RCCTX0070 Paracetamol RCCTX0004 Salicylate RCCTX0004 Propranolol RCCTX0020 ssessment Manager: MH Page 8 of 13

9 HUMN ODY FLUIDS iochemistry examination clinical diagnosis In house assays using gilent 5575 GCMS and SOPs: - Serum, lood, Urine Morphine RCCTX0063 ine RCCTX0063 Dihydrocodeine RCCTX0063 6MM RCCTX0063 Cocaine RCCTX0023 enzoylecgonine RCCTX0023 mphetamine RCCTX0038 Methyl mphetamine RCCTX0038 MDM RCCTX0038 MD RCCTX0038 arbiturates RCCTX0072 Chlorpromazine Cyclizine Nefopam Procyclidine Citalpram Mirtazapine Sertraline Venlafaxine Diphenhydramine Methadone ssessment Manager: MH Page 9 of 13

10 HUMN ODY FLUIDS iochemistry examination clinical diagnosis In house assay using Uvikon Spectrophotometer and SOP lood, Serum, Urine Carboxyhaemoglobin RCCTX0014 Paraquat RCCTX0045 Diaquat RCCTX0045 In house assay using thermo Fisher TSQ Ultra LCMS and SOP lood Cyclosporin CCTEND013 Tacrolimus CCTEND013 Sirolimus CCTEND013 Plasma Serum Serum Serum CTH C-Peptide DHES hcg Siemens Immunlite 2000 Chemiluminescent assay SOP RCCEND 003 ssessment Manager: MH Page 10 of 13

11 HUMN ODY FLUIDS iochemistry examination clinical diagnosis Thermo Fisher U3000 HPLC using iorad kits and SOP Urine drenaline CCNTHPLC003 Noradrenaline CCNTHPLC003 Metadrenaline CCNTHPLC003 Nor metadrenaline CCNTHPLC003 Dopamine CCNTHPLC003 5 HI CCNTHPLC004 Serum Vitamin SC005 Vitamin E SC005 In house assay using Thermo Fisher TSQ Ultra LCMS and SOP 17-hydroxy progesterone ndrostenedione RCCTX7099 RCCTX7099 In house assays using Perkin Elmer 600 tomic bsorption and SOP S lood, Urine Mercury CCNTTMET009 Lead CCNTTMET007 Cobalt CCNTTMET011 ssessment Manager: MH Page 11 of 13

12 HUMN ODY FLUIDS iochemistry examination clinical diagnosis In house assay ICP-MS using gilent 7700 Serum Copper CCNTTMET020 Selenium CCNTTMET020 Zinc CCNTTMET020 luminium CCNTTMET021 Urine Copper CCNTTMET002 Serum IGF-1 Mediagnost radio immunoassay using SOP RCCENDM0051 Serum one Specific lkaline Phosphatase Microvue ELIS kit and SOP CCTENDM006 Serum lkaline Phosphatase Isoenzymes Helena Iso electric focusing electrophoresis and SOP RCCENDM0019 Urine hcg In house radio immunoassay and SOP RCCENDM0015 Faeces Pancreatic Elastase Schebo ELIS kit and SOP RCCENDM0009 Urine hcg (pregnancy test) lere hcg lateral flow cassette test and SOP RCCENDM0026 Serum, Urine Osmolality Vitech osmometer using freezing point depression and SOP CCNUTO021 CSF ilirubin Uvikon scanning Spectrophotometer with ioc software and SOP CCTENDM014, ssessment Manager: MH Page 12 of 13

13 HUMN ODY FLUIDS iochemistry examination clinical diagnosis L90 lood gas analyser and SOP CCNUTO113 lood ph po2 pco2 Sodium Potassium Chloride Ionised Calcium Glucose Lactate Haemoglobin fractions L80 blood gas analyser and SOP CCNUTO113 END ssessment Manager: MH Page 13 of 13

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