INFLUENCE OF CORDYCEPS SINESIS EXTRACT ON FERMENTATION PROCESS
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1 INFLUENCE OF CORDYCEPS SINESIS EXTRACT ON FERMENTATION PROCESS Kristina Nešić, Saša Despotović*, Milena Savić, Anita Klaus, Ida Leskošek-Čukalović, Viktor Nedović and Miomir Nikšić University of Belgrade-Faculty of Agriculture, Belgrade, Serbia
2 O B J E C T I V E S Developing novel beer based products with health-promoting properties. Using mushrooms as a natural source of nutritional supplements. Influence of Cordyceps extract on growth of yeast s cells. Influence of Cordyceps extract on fermentation process.
3 WHAT IS CORDYCEPS SINESIS? o Edible and medicinal fungi o Used in Asia for thousand of years o Parasites larvae of ghost moths
4 o The fungus germinates in the living larva o Kills and mummifies it o Stalk-like fruiting body emerges from the corpse
5 CORDYCEPS SINESIS
6 Cordyceps Sinensis common names Ophiocordyceps sinensis Caterpillar fungus Deer fungus Tochukaso Tong Chong Xia Cao "winter worm, summer grass"
7 Where it can be found? Found naturally above 3000 meters above sea level. In highlands of China Tibet Nepal Industrially produced
8 NUTRITIONAL CONTENTS OF CORDYCEPS Hydroxy-Ethyl Adenosine Cordyceptic acid Cordycepin Amino acids Polysaccharides Vitamin B1,B3,B12
9 HEALTH BENEFITS OF CORDYCEPS Replenishment of health Preventing aging Improving physical performance Increases Energy ATP Production 30% Oxygen Utilization 40%
10 HEALTH BENEFITS OF CORDYCEPS Night sweating Decreases Appetite Decreases Alcohol Desire Respiratory disease Arrhythimias and other heart diseases.
11 HEALTH BENEFITS OF CORDYCEPS Regulates blood sugar Strong anti-oxidation activity Antitumor activity Immuno-modulating activity Increases libido & sexual function
12 Blood Sugar Regulation with Cordyceps
13 BEER
14 WHY BEER? 14
15 More facts about Beer - MOST POPULAR ALCOHOLIC BEVERAGE - NATURAL PRODUCT - POSSESS MICRO AND MACRO NUTRIENTS - PROTEINS - CARBOHYDRATES - VITAMINS - AROMATIC AND BITTER COMPONENTS - ALCOHOL...
16 Beer is : increasing HDL lowering insulin level acting as blood thinner reduce the risk of developing kidney stones and flush kidneys increase bone density relaxing effect on the body
17 MATERIALS AND METHODS
18 Cordyceps sinesis - extract (Cordyceps Super TM by Aloha Medicinals) Wort from local brewery Yeast - Saccharomyces pastorianus local brewery - Saccharomyces cereviseae baker s yeast - Sacharomyces cereviseae K1 wine yeast
19 A-sample with beer yeast and Cordyceps, B sample with beer yeast, C- sample with wine yeast and Cordyceps, D- sample with wine yeast, E- sample with baker yeast and Cordyceps, D- sample with baker yeast
20 C.synesis /water -10 ml/l Fermentation Cooling at 10 C Sterilization of wort Pitching - 20 ml/l yeast suspension (10 7 cells/ml )
21 ANALYSIS o Beer Alcolyzer (Anton Paar) o Alcohol o Real extract o Degree of fermentation
22 RESULTS AND DISCUSSION
23 Alcohol (%) Cordyceps extract in concentration 50 mg/ml - beer yeast 4,50% 4,00% 3,50% 3,00% 2,50% 2,00% 1,50% 1,00% 0,50% 0,00% Sample Control Fermentation time (h)
24 Alcohol (%) Cordyceps extract in concentration 50 mg/ml wine yeast 4,50% 4,00% 3,50% 3,00% 2,50% 2,00% 1,50% 1,00% 0,50% Sample Control 0,00% Fermentation time (h)
25 Alcohol (%) Cordyceps extract in concentration 50 mg/ml Baker s yeast 5,00% 4,50% 4,00% 3,50% 3,00% 2,50% 2,00% 1,50% 1,00% 0,50% 0,00% Sample Control Fermentation time (h)
26 Alcohol (%) Cordyceps extract in concentration 50 μg/ml - beer yeast 4,50% 4,00% 3,50% 3,00% 2,50% 2,00% 1,50% 1,00% 0,50% Sample Control 0,00% Fermentation time (h)
27 Alcohol (%) Cordyceps extract in concentration 50 μg/ml wine yeast 4,50% 4,00% 3,50% 3,00% 2,50% 2,00% 1,50% 1,00% 0,50% Sample Control 0,00% Fermentation time (h)
28 Alcohol (%) Cordyceps extract in concentration 50 μg/ml Baker s yeast 4,50% 4,00% 3,50% 3,00% 2,50% 2,00% 1,50% 1,00% 0,50% 0,00% Sample Control Fermentation time (h)
29 CONCLUSIONS Cordyceps extract in concentration of 50 mg/ml significantly increased fermentation rate when used with beer and wine yeast, while when used with baker s yeast there was no significant increase of fermentation rate. Cordyceps extract in concentration of 50 μg/ml significantly increased fermentation rate when used with beer yeast, while when used with wine and baker s yeast there was no significant increase of fermentation rate.
30 THANK YOU AND CHEERS!
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