This annex is valid from: to Replaces annex dated: Location(s) where activities are performed under accreditation

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1 Normative document: EN IS/IEC 17025:2005 Registration : L 106 SYNLAB Analytics & Services osterhout B.V. (s) where activities are performed under accreditation Head ffice Everdenberg TT osterhout Nederland Abbreviation/ location code Everdenberg TT osterhout Nederland Sampling a. Water Sampling for Legionella analyses LGI030M in accordance with NEN 6265:2007 b. Drinking water, groundwater (Matrix A) Sampling for Legionella testing with internal reference MIC417M. LGI030M in accordance with NEN-EN-IS en NEN-EN-IS Process water, water from cooling towers, swimming pool water (Matrix B) This annex has been approved by the Board the Dutch Accreditation Council, on its behalf, J.A.W.M. de Haas Director perations 1 If there is a referral to a code starting with NAW, NAP, EA or IAF, this concerns a scheme mentioned on the RvA-BR010-lijst. If no date or version is mentioned for a normative document, the accreditation concerns the most current version the document or scheme. Dutch Accreditation Council RvA Page 1 8

2 Normative document: EN IS/IEC 17025:2005 Registration : L 106 SYNLAB Analytics & Services osterhout B.V. c. Water Sampling for microbiological analyses LGI030M in accordance with IS d. Swimming pool water Sampling for inorganic, organic and microbiological analyses LGI030M in accordance with NEN-EN-IS Microbiological analyses 1. Water Determining the amount Legionella bacteria; including serotyping; filtration method, GVPC, BCYE without L-cysteine, BCYE with L-cysteine 2. Drinking water Determining the amount Legionella bacteria; including serotyping; filtration method, BCYE without L-cysteine, BCYE with L-cysteine, BCYE with antibiotics 3. Legionella isolates Serotyping Legionella bacteria; latex agglutination test MIC303W in accordance with NF T MIC083W in accordance with NEN 6265:2007 (serotyping: MIC283W; ) MIC417M 4. Drinking water, groundwater (Matrix A) Enumeration Legionella; membrane filtration, medium A, B MIC417M in accordance with NEN-EN-IS (procedure 8,9,10) 5. Process water water from cooling towers, swimming pool water (Matrix B) Enumeration Legionella; membrane filtration, medium C (GVPC) MIC417M in accordance with NEN-EN-IS (procedure 8,9,10) 6. Drink and surface water Determining the total aerobic count at 22 C and 36 C; Pour plate method, YEA MIC336W in accordance with IS Determining the amount coliforms; filtration method, MLSA with confirmation 8. Determining the amount Escherichia coli; filtration method, MLSA with confirmation MIC304W in accordance with IS (1990) MIC304W in accordance with IS (1990) 9. Determining the amount sulphite reducing Clostridia spores; filtration method, TSC MIC338W equivalent to NEN 6567 (1985) Dutch Accreditation Council RvA Page 2 8

3 Normative document: EN IS/IEC 17025:2005 Registration : L 106 SYNLAB Analytics & Services osterhout B.V. 10. Drink and surface water Determining the amount enterococci; filtration method, Slanetz and Bartley MIC337W equivalent to IS Foods (except milk and milk s) and animal Determining the amount coliforms; pour plate method, VRBL, 37 C, without confirmation MIC018W in accordance with IS Foods and animal Determining the total aerobic count at 30 C; pour plate method, PCA MIC004W in accordance with IS Determining the amount thermo tolerant coliforms; pour plate method, VRBL, 44 C, without confirmation MIC018W 14. Foods (except milk and raw milk cheese) and animal and manure from poultry Detection Salmonella spp.; VIDAS method, VIDAS UP Salmonella protocol MIC409W equivalent to IS 6579 AFNR BI 12/32-10/ Milk and milk s Determining the amount coliforms; pour plate method, VRBL, 30 C, with confirmation MIC018W in accordance with IS Foods Determining the amount Enterobacteriaceae; pour plate method, VRBG without confirmation MIC005W in accordance with NEN-IS Determining the amount coagulase positive Staphylococci; spread plate method, BP with confirmation MIC040W in accordance with IS Determining the amount Escherichia coli; pour plate method, TBX 19. Determining the amount yeast and moulds; pour plate method, CGA 20. Determining the amount Bacillus cereus; spread plate method, MYP with confirmation 21. Determining the amount Listeria monocytogenes; spread plate method, ALA with confirmation MIC031W in accordance with IS MIC033W in accordance with IS 7954 (1987) MIC027W in accordance with NEN-EN-IS 7932 MIC044W in accordance with NEN-EN-IS /A1 Dutch Accreditation Council RvA Page 3 8

4 Normative document: EN IS/IEC 17025:2005 Registration : L 106 SYNLAB Analytics & Services osterhout B.V. 22. Foods Determining the amount lactic acid bacteria; pour plate method, MRSA 23. Determining the amount Clostridium perfringens; pour plate method, TSC with confirmation 24. Detection Listeria monocytogenes; VIDAS method, VIDAS LMX protocol MIC035W in accordance with NEN-IS MIC234W in accordance with IS 7937 MIC358W equivalent to NEN-EN-IS AFNR 12/27-02/ Foods and process water Detection Salmonella spp.; VIDAS method, VIDAS easy SLM protocol MIC361W equivalent to IS 6579 AFNR BI 12/16-09/ Salmonella isolates Serotyping Salmonella; agglutination according White-Kauffmann-Le Minor scheme S. typhimurium, S. typhimurium (monasisch), S. MIC172W conform IS enteritidis, S. infantis, S. virchow, S. hadar, S. agona, S. paratyphi B var. Java Dutch Accreditation Council RvA Page 4 8

5 Normative document: EN IS/IEC 17025:2005 Registration : L 106 SYNLAB Analytics & Services osterhout B.V. 27. Salmonella isolates Serotyping Salmonella; PCR (Check & Trace) S. aberdeen, S. abony, S. adelaide, S. agona, S. alachua, S. albany, S. altona, S. amsterdam, S. anatum, S. augustenborg, S. banana, S. bareilly, S. berta, S. blockley, S. bongori, S. bovismorbificans, S. braenderup, S. brandenburg, S. bredeney, S. brunei, S. carno, S. carrau, S. cerro, S. chandans, S. chester, S. choleraesuis, S. coeln, S. colindale, S. corvallis, S. cubana, S. derby, S. dublin, S. duisburg, S. eboko, S. enteritidis, S. gallinarum gallinarum, S. gallinarum pullorum, S. give, S. gloucester, S. goldcoast, S. grumpensis, S. hadar, S. havana, S. heidelberg, S. ibadan, S. idikan, S. indiana, S. infantis, S. isangi, S. jangwani, S. jaiana, S. kedougou, S. kentucky, S. kottbus, S. lexington, S. lille, S. litchfield, S. liverpool, S. livingstone, S. london, S. manchester, S. manhattan, S. matadi, S. mbandaka, S. meleagridis, S. michigan, S. mikawasima, S. minnesota, S. monschaui, S. montevideo, S. muenchen, S. muenster, S. napoli, S. newport, S. ohio, S. oranienburg, S. orion, S. oslo, S. ouakam, S. panama, S. paratyphi A, S. paratyphi B, S. paratyphi B v. Java, S. paratyphi C, S. pomona, S. poona, S. reading, S. regent, S. rissen, S. rubislaw, S. saintpaul, S. sandiego, S. schwarzengrund, S. senftenberg, S. soerenga, S. stanley, S. stockholm, S. stourbridge, S. telelkebir, S. tennessee, S. thompson, S. typhi, S. typhimurium, S. uganda, S. urbana, S. veneziana, S. virchow, S. wandsworth, S. weltevreden, S. worthington, S. yoruba., S. Eastbourne, S. Hartford, S. Hvittingfoss, S. Kapemba, S. Kirkee, S. Lagos, S. Llandf, S. Ruiru, S. Taksony. MIC424W 28. Poultry and poultry s Detection Campylobacter spp.; VIDAS CAM protocol MIC429W equivalent to EN-IS ; AFNR BI 12/29-05/ Meat and meats, potatoes, vegetables and fruit, water Detecting Shigatoxin producing E. coli (STEC), screeningprocedure on stx and eae genes; qualitative real time PCR-technique 30. Detecting Shigatoxin producing E. coli (STEC), screeningprocedure on 26, 103, 111, 145 and 157; qualititative real time PCR-technique MIC435W in accordance with IS/TS MIC435W in accordance with IS/TS Dutch Accreditation Council RvA Page 5 8

6 Normative document: EN IS/IEC 17025:2005 Registration : L 106 SYNLAB Analytics & Services osterhout B.V. 31. Foods Determining the amount Listeria monocytogenes; pour plate method, ALA with confirmation MIC602W equivalent to NEN-EN-IS /A1 Inorganic analyses 32. Fats and oils Determination free fatty acids (direct); 33. Foods Determination free fatty acids (after extraction); 34. Fats and oils Determination perioxide value (direct); 35. Foods Determination peroxide value (after extraction); 36. Fats and oils Determination free fatty acids (direct); 37. Foods Determination free fatty acids (after extraction); 38. Fats and oils Determination perioxide value (direct); 39. Foods Determination peroxide value (after extraction); CHE080W equivalent to NEN-EN-IS 660 CHE080W extraction ; equivalent to NEN-EN-IS 660 CHE078W equivalent to NEN-EN-IS CHE078W extraction ; equivalent to NEN-EN-IS CHE080W equivalent to NEN-EN-IS 660 CHE080W extraction ; equivalent to NEN-EN-IS 660 CHE078W equivalent to NEN-EN-IS CHE078W extraction ; equivalent to NEN-EN-IS Foods and animal Determining the raw ash content; gravimetric CHE005W 41. Foods (wet) Determining the moisture content; dry oven method, drying temperature 103 C by use sand and alcohol CHE052W / CHE003W 42. Foods and animal (dry) Determining the moisture content; dry oven method, drying temperature 103 C CHE052W Dutch Accreditation Council RvA Page 6 8

7 Normative document: EN IS/IEC 17025:2005 Registration : L 106 SYNLAB Analytics & Services osterhout B.V. 43. Cereals and cereal s, legumes and legume s Determining the fat content; petroleumether- / hexane extraction, gravimetric, direct extraction (without pre drying) CHE004W 44. Meat and meat s Determining the fat content; petroleumether- / hexane extraction, gravimetric, direct extraction (with pre drying) CHE003W 45. Foods and animal Determining the fat content; petroleumether- / hexane extraction, gravimetric, extraction after acid hydrolysis CHE004W 46. Potato- and fruit s and drinks Determining the sulphite (S2) content; CHE093W 47. Potato-, vegetableand fruit s, bread and confectionery Determining the dietary fibre content; enzymatic CHE060W 48. Foods and animal (except meats and meat s) Determining the starch content; polarimetry CHE063W 49. Meat and meat s Determining the starch content; polarimetry CHE062W 50. Extracts vegetable- and fruit s and syrups Determining the refractive index (refractometer value); refractometry CHE008W 51. Foods and animal Determining the protein content; Dumas-N CHE014W rganic analyses 52. Foods Determining the total sugar content (based on fructose, glucose, lactose, maltose, sucrose); HPLC RI 53. Determination mycotoxins: Deoxynivalenol; Zearalenon; HPLC-MS/MS CHE001W CHE602W Dutch Accreditation Council RvA Page 7 8

8 Normative document: EN IS/IEC 17025:2005 Registration : L 106 SYNLAB Analytics & Services osterhout B.V. 54. Foods and animal Determining the B1-, B2-, G1- en G2- aflatoxine content; HPLC fluorescence CHE022W equivalent to NEN-EN-IS Determining the chratoxine A content; HPLC fluorescence 56. Determination mycotoxins: chratoxin A; Aflatoxin B1, B2, G1, G2; HPLC-MS/MS 57. Foods Determining the benzoic acid and/or sorbic acid content; HPLC UV CHE210W CHE602W CHE013W 58. Determining the fatty acid prile; GC-FID CHE061W 59. Foods and water Determination Acrylamide (HPLC-MS/MS) CHE587W 60. Fish and fish s, shellfish Determination Histamine (HPLC-MS/MS) CHE589W Dutch Accreditation Council RvA Page 8 8