Finnish Food Safety Authority Evira NRL Salmonella in primary production, food and feed in Finland

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1 Finnish Food Safety Authority Evira NRL Salmonella in primary production, food and feed in Finland Activities to fulfill tasks and duties DVM Henry Kuronen EURL-Salmonella workshop Uppsala

2 NRL-Salmonella in Finland NRL-Salmonella laboratories for non-human isolates are part of Research and Laboratory Services Department of Evira: Veterinary Bacteriology and Pathology Recearch Unit, Kuopio (accredited according ISO 17025) Sampling and analysing methods concerning samples from primary production (faeces, environmental samples etc.) Confirmation and serotyping of isolates from animals, foods and feeds Microbiology Research Unit, Helsinki, (accredited according ISO 17025) Sampling and analysing methods concerning samples from food and feed; analyses of feed and fertilizer samples National Institute for Health and Welfare (THL) is the reference laboratory for human samples and strains in Finland EURL-Salmonella Workshop Uppsala, DVM Henry Kuronen 2

3 EURL-Salmonella Workshop Uppsala, DVM Henry Kuronen 3

4 Evira operating locations EURL-Salmonella Workshop Uppsala, DVM Henry Kuronen 4

5 Our laboratory building in Kuopio EURL-Salmonella Workshop Uppsala, DVM Henry Kuronen 5

6 Staff in our salmonellalaboratory EURL-Salmonella Workshop Uppsala, DVM Henry Kuronen 6

7 Laboratory activities in Kuopio Serotyping about isolates annually sent to NRL 6 18 different serotypes from samples of domestic food production animals per animal species during years about 90 serotypes from other samples (imported food, sewage, compost etc.) during years resistance monitoring PFGE typing epidemiological investigations laboratory cross-contaminations Ongoing implementation of WGS Own sequencing equipments in Helsinki Harmonization with THL (National Institute for Health and Welfare) EURL-Salmonella Workshop Uppsala, DVM Henry Kuronen 7

8 Guidance of the official laboratories Organization of annual meetings for laboratories Presentations in training courses for laboratories staff Reference Laboratory Newsletter about 4 times / year (including not only salmonella) subjects: detection methods, processing of samples, information from EURL:s etc. Own working group in Evira for reference laboratory activities Lead by the head of Quality Assurance of the Laboratory Services EURL-Salmonella Workshop Uppsala, DVM Henry Kuronen 8

9 Official laboratories At the moment 22 municipal or commercial laboratories have been approved to do the analyses The suspected isolates have to be sent to NRL for confirmation and serotyping The official aboratories have to be accredited according to the ISO For primary production samples ISO :2017 method (MSRV) EURL-Salmonella Workshop Uppsala, DVM Henry Kuronen 9

10 Interlaboratory Comparison Studies (1) The Scandinavian Interlaboratory comparison study on detection of Salmonella in material from animal production, organised by SVA, Sweden Finnish laboratories have participated since 2010 laboratories are required to participate to the study at least every third year 2018 study (the 11 th Study), 10 g pig faeces per sample 4 blanks (4 / 4) 7x S. Derby ca.59 cfu ( 5 of 7) 7x S. Enteritidis ca. 64 cfu ( 5 of 7) 7x S. Typhimurium (no H 2 S) ca. 34 cfu ( 5 of 7) good performance in total 20 (and results in the brackets) More information about the studies in EURL-Salmonella websides EURL-Salmonella workshop 2016, St Malo, Lennart Melin EURL-Salmonella Workshop Uppsala, DVM Henry Kuronen 10

11 Interlaboratory Comparison Studies (2) Follow-up if not good performance in SVAs study Contact by and guiding the laboratory for example power point about the possible problems with MSRV method checking the procedures and making the possible corrections => extrasamples Food: laboratories are required to participate the profiency testing at least once per four years Mainly the scheme organised by the National Food Agency of Sweden Most of the laboratories are the same which are analysing also the primary production samples EURL-Salmonella Workshop Uppsala, DVM Henry Kuronen 11

12 Study organised by SVA (primary production) Total number of participated Finnish laboratories during per year (altogether 38) In this table the 22 laboratories which are approved at this moment Laboratory X c X X 4 X X X i X 7 X 8 X X X X 11 X X X 14 X X 15 X X X h 16 X X g X j 17 X X X j 18 X 20 X 21 X X 22 X X 23 X X X 28 X X X 30 X X X i 31 X a X X X X X X X 32 X f X X X 34 X X X 35 X X X j 37 X b X X X 38 X X i Total

13 Other activities (1) Collaboration with The National Institute for Health and Welfare THL in scientific research THL: phagetyping Enteritidis and Typhimurium strains also from animal, food and feed origin phagetyping until to the end of 2018 WGS implementation comparison of isolates from human, animal and food origin Virtual Zoonoosis Centre experts from Evira and THL Collalaboration with the Ministry of the Agriculture and Forestry (regulation drafts etc.) EURL-Salmonella Workshop Uppsala, DVM Henry Kuronen 13

14 Other activities (2) Collalaboration with the Animal Health ETT the association promotes the health and welfare of foodproducing animals by coordinating national animal health care and steering the import of production animals as well as their embryos, semen and feeds Participation to the EURL-Salmonella studies of serotyping and detection of salmonella in animal faeces (Kuopio) and in food and feed (Helsinki) EURL-Salmonella workshops and other collalaboration with EURL-Salmonella (request of information of serotypes etc.) Nordic co-operation in revising national salmonella control programmes EURL-Salmonella Workshop Uppsala, DVM Henry Kuronen 14

15 The Finnish Salmonella Control Programme the control of salmonella in production animals and products of animal origin has been carried out decades (from s) EU accepted the Finnish Salmonella Control Programme originally1995 (revised some times) concerns live cattle, swine and poultry, fresh meat of cattle, swine and poultry and eggs feed control is part of the programme all serotypes are notifiable vaccination or treating by antibiotics are not allowed aim is to maintain the very low prevalence of salmonella EURL-Salmonella Workshop Uppsala, DVM Henry Kuronen 15

16 Serotypes and number of faecal positive cattlefarms Altona Coeln Derby Eastbourne Enteritidis Goldcoast Haifa Hessarek Infantis Konstanz Livingstone Minnesota Montevideo Muenchen Panama Rissen S. ssp. IIIb 50:z10:z Typhimurium EURL-Salmonella Workshop Uppsala, DVM Henry Kuronen 16

17 Serotypes and number of faecal positive pig farms Bovismorbificans Derby Mbandaka Stockholm Tennessee Typhimurium EURL-Salmonella Workshop Uppsala, DVM Henry Kuronen 17

18 Serotypes and number of salmonella positive poultryflocks Laying hens Broilers Anatum Enteritidis Heidelberg Plymouth S. ssp. II (=salamae) Tennessee Typhimurium Turkeys Albany Cerro Infantis Livingstone Montevideo Nuorikkala Enteritidis Infantis Tennessee Typhimurium Newport Poona Tennessee Typhimurium EURL-Salmonella Workshop Uppsala, DVM Henry Kuronen 18