RVA starch pasting profiles and Promatest protein solubilities of grain sorghums
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1 RVA starch pasting profiles and Promatest protein solubilities of grain sorghums Ha H Truong, Ali Khoddami, Thomas H. Roberts, Sonia Yun Liu and Peter H Selle
2 Weighbridge assessments of feed grain quality Maize Wheat White sorghum Red sorghum White corn RVA starch pasting profiles Promatest protein solubility Texture (Symes PSI) NIR amino acids AusScan
3 Rapid-Visco Analysis (RVA) starch pasting profiles 4.2g sorghum g deionised H 2 O Slurry held at 50 C for 1 minute heated 95 C/2.5 minutes Peak + Holding cp cooled 50 C/2 minutes Final cp
4 Sorghum and wheat RVA starch pasting profiles sorghum wheat Time (seconds) 13 minutes
5 Sorghum and wheat RVA starch pasting profiles Peak sorghum Final wheat Holding Breakdown: Peak Holding Setback: Final - Holding Time (seconds) 13 minutes
6 RVA starch pasting profiles of the six Liverpool Plains sorghums Peak (cp): Min 2455, Mean 4759, Max 6857 RVA profiles of 8 sorghums evaluated in RIRDC sorghum starch project Peak (cp): Min 2392, Mean 4702, Max 9511
7 Cherry-picked correlations between Peak, Holding and Final RVA viscosities and bird performance parameters from RIRDC sorghum starch study #4 Item r Significance Peak- AME Peak-ME:GE Peak-AMEn Holding-FCR Holding-Starch PJ Holding-Starch:Prot. dis. PJ Final-FCR Final-N retention Final-Starch dig. PJ Final-Protein dig. PJ Final-Starch:Prot. dis PJ r = r = r = r = r = r = r = r = r = r = r = P = P = P = P = P = P = P = P = P = P = P = 0.007
8 RVA starch pasting profiles of the six sorghums Starch digestibility
9 AME (MJ/kg DM) RVA viscosity is correlated to AME 12.8 Sorghums: MP JM HP Tiger HFQ R² = Holding RVA viscosity of sorghum (cp)
10 Final RVA viscosity is correlated to FCR (r = ; P < 0.05)
11 Hence RVA starch pasting properties may be indicative of broiler performance on Pros sorghum-based diets -Cheap and quick measurement -A more in depth understanding of starch characteristics than amylose:amylopectin ratios Cons -May only be indicative in sorghum? -Unknown: the effects of other feed ingredients on sorghum starch
12 Na metabisulphite had a profound effect on RVA starch pasting properties presumably as the result of oxidative-reductive depolymerisation (ORD) of starch polysaccharides Effect of Na metabisulphite [0 and 5.25 g/kg] on RVA viscosities of starch extracted from sorghum-based diets Peak viscosity cp -26.2% Final viscosity cp -57.4% Peak With 5.25 g/kg SMBS Peak Final Final
13 Effect of Na metabisulphite [0 to 5.25 g/kg] on final RVA viscosities of starch extracted from sorghum-based diets SMBS reduces RVA starch pasting properites while increases protein solubility better access of amylase to starch?
14 Promatest protein solubility A weighbridge assessment of grain sorghum in chooks? The protein solubilities of the white and red sorghums per se and the six experimental diets were determined. The Promatest method was used, which compares the solubility of extracted protein with albumin as the 100% soluble standard as described by Odjo et al. (2012). Odjo S, Malumba P, Dossou J, Janas S, Bera F (2012) Influence of drying and hydrothermal treatment of corn on the denaturation of saltsoluble proteins and color parameters. Journal of Food Engineering 109,
15 Promatest protein solubility Protein solubility of six red sorghums ex Liverpool Plains, 2009 harvest The Promatest method compares the solubility of extracted protein with albumin as the 100% soluble standard (Odjo et al. 2012).
16 Kafirin concentrations in sorghum negatively correlated with protein solubility Promatest protein solubility (%) R² = Kafirin (g/kg)
17 AMEn (MJ/kg) AME (MJ/kg) ME:GE ratio Promatest protein solubility is correlated with AME, AMEn and ME:GE ratios R² = Promatest protein solubility (%) R² = Promatest protein solubility (%) R² = Promatest protein solubility (%)
18 SMBS increases Promatest protein solubility Responses from 34.8 to > 60%
19 Promatest protein solubility (%) Peak RVA viscosity (cp) Kafirin may depress peak RVA viscosity and Promatest protein solubility of grain sorghums Kafirin (g/kg) R² = P = R² P = = Kafirin (g/kg)
20 Conclusion RVA starch pasting profiles, in combination with Promatest protein solubilities may determine feed grain quality (including digestive dynamics) in broiler chickens. Further R&D is justified but, fortunately, will be completed
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