RESEARCH EXPERIENCE UNIVERSITY OF CALIFORNIA SAN FRANCISCO, CA, USA

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1 Curriculum Vitae PURAN SINGH SIJWALI, Ph.D. Principal Scientist, Center for Cellular and Molecular Biology Uppal Road, Hyderabad , India Phone (O) ; (Cell) Fax: / ; E mail: psijwali@ccmb.res.in RESEARCH INTEREST My lab is investigating roles of proteases in stage-specific development and differentiation of malaria parasite. The goal is to identify proteases that have essential functions in parasite development and are parasite-specific or significantly different from the host homologs, hence, can be targeted for development of new antimalarials. Another area of investigation is host pathogen interactions in which we are focusing on variant antigens of malaria parasites. EDUCATION M.Sc. (Govind Ballabh Pant University of Agriculture & Technology, Pantnagar, India) 1994 Major. Molecular Biology and Biotechnology Thesis. Enzyme-immuno assay method for detection and quantitation of Heliothis armigera nuclear polyhedrosis virus Ph.D International Centre for Genetic Engineering and Biotechnology-Jawaharlal Nehru University, New Delhi, India Thesis. Cloning and characterization of the thrombospondin-related adhesive protein (TRAP) from the simian and the human malaria parasites RESEARCH EXPERIENCE UNIVERSITY OF CALIFORNIA SAN FRANCISCO, CA, USA Assistant Researcher III May 2006 to April 2008 Assistant Researcher II May 2004 to May 2006 Postgraduate Researcher July 2002 to May 2004 Visiting Post-Doctoral Research Scholar August 1999 to June 2002 AWARDS Integrated scholarship for High School 1984 to 1986 Scholarship for M.Sc. from DBT 1991 to 1994 Research fellowship for Ph.D. from CSIR 1994 to 1999 Travel Scholarship for presentation at the Molecular Approaches to Malaria conference, February 2012, Australia Travel Award for presentation in the Gordon Research Conference on malaria. 2009, Oxford, UK. Ph.D. STUDENTS: Supervised 3 students Puran S. Sijwali Page 1

2 PUBLICATIONS 1. Ashok Yadav P, Pavan Kumar C, Siva B, Suresh Babu K, Allanki AD, Sijwali PS, Jain N, Veerabhadra Rao A. (2017) Synthesis and evaluation of anti-plasmodial and cytotoxic activities of epoxyazadiradione derivatives. Eur J Med Chem. 134: doi: /j.ejmech Al-Nihmi FM, Kolli SK, Reddy SR, Mastan BS, Togiri J, Maruthi M, Gupta R, Sijwali PS, Mishra S and Kumar KA. (2017) A Novel and conserved Plasmodium sporozoite membrane protein SPELD is required for maturation of exo-erythrocytic forms. Sci Rep. 2017; 7: doi: /srep Balabadra S, Kotni M, Manga V, Allanki AD, Prasad R and Sijwali PS. (2016) Synthesis and evaluation of naphthyl bearing 1,2,3-triazole analogs as antiplasmodial agents, cytotoxicity and docking studies. Bioorg Med Chem. 2017;25(1): doi: /j.bmc Kolla VK, Prasad R, Sayyad Z, Atul, Shah AY, Allanki AD, Navale R, Singhal N, Tanneru N, Sudhakar R, Venkatesan V, Deshmukh MV and Sijwali PS. (2015) Independent amino acid residues in the S2 pocket of falcipain-3 determine its specificity for P2 residues in substrates. Mol Biochem Parasitol. 202(2): doi: /j.molbiopara Chandra Shekhar A, Lingaiah BP, Shanthan Rao P, Narsaiah B, Allanki AD and Sijwali PS. (2015) Design, Synthesis and Biological Evaluation of Novel Fluorinated Heterocyclic Hybrid Molecules Based on Triazole & Quinoxaline Scaffolds Lead to Highly Potent Antimalarials and Antibacterials. Letters in Drug Design & Discovery 12 (5): Navale R, Atul, Allanki AD and Sijwali PS. (2014) Characterization of the autophagy marker protein Atg8 reveals atypical features of autophagy in Plasmodium falciparum. PLoS One 9(11): e doi: /journal.pone Singhal N, Atul, Mastan B, Arun Kumar K and Sijwali PS. (2014) Genetic ablation of plasmodj1, a multi-activity enzyme, attenuates parasite virulence and reduces oocyst production. Biochem. J. 461 ( ). 8. Chandra Shekhar A, Shanthan Rao P, Narsaiah B, Allanki AD and Sijwali PS. (2014) Emergence of pyrido quinoxalines as new family of antimalarial agents. Eur J Med Chem. 77C: Prasad R, Atul, Kolla VK, Legac, J, Singhal N, Navale, R, Rosenthal PJ and Sijwali PS. (2013) Blocking Plasmodium falciparum development via dual inhibition of hemoglobin degradation and the ubiquitin proteasome system by MG132. PLoS One. 8(9):e doi: /journal.pone Tadigoppula N, Korthikunta V, Gupta S, Kancharla P, Khaliq T, Soni A, Srivastava RK, Srivastava K, Puri SK, Raju KS, Wahajuddin, Sijwali PS, Kumar V and Mohammad IS. (2013) Synthesis and insight into the structure-activity relationships of chalcones as antimalarial agents. J Med Chem Jan 10;56(1): Prasad R, Atul, Soni A, Puri SK and Sijwali PS. (2012) Expression, Characterization, and Cellular Localization of Knowpains, Papain-Like Cysteine Proteases of the Plasmodium knowlesi Malaria Parasite. PLoS One. 7(12):e Sundararaj S, Singh D, Saxena AK, Vashisht K, Sijwali PS, Dixit R and Pandey KC. (2012) The Ionic and Hydrophobic Interactions Are Required for the Auto Activation of Cysteine Proteases of Plasmodium falciparum. PLoS One. 7(10):e Sijwali PS and Rosenthal PJ. (2010) Functional evaluation of Plasmodium export signals in Plasmodium berghei suggests multiple modes of protein export. PLoS One 19;5(4): e Puran S. Sijwali Page 2

3 14. *Subramanian S, *Sijwali PS and Rosenthal PJ. (2007) Falcipain cysteine proteases require bipartite motifs for trafficking to the Plasmodium falciparum food vacuole. Journal of Biological Chemistry 282(34): *Equal Contribution. 15. Rosenthal PJ, Singh A, Walker, KJ, Sijwali PS and Lau A. (2007) A chimeric cysteine protease of Plasmodium berghei engineered to resemble the Plasmodium falciparum protease falcipain- 2. Protein Engineering, Design and Selection 20(4): Sijwali PS, Koo J, Singh N and Rosenthal PJ. (2006) Gene disruptions demonstrate independent roles for the four falcipain cysteine proteases of Plasmodium falciparum. Mol Biochem Parasitol 150(1): 96: Wang SX, Pandey KC, Somoza JR, Sijwali PS, Kortemme T, Brinen LS, Fletterick RJ, Rosenthal PJ, and McKerrow JH. (2006) Structural basis for unique mechanisms of folding and hemoglobin binding by a malarial protease. Proceedings of the National Academy of Sciences USA 103(31): Parikh S, Liu J, Sijwali P, Gut J, Goldberg DE and Rosenthal PJ. (2006) Antimalarial effects of human immunodeficiency virus type 1 protease inhibitors differ from those of the aspartic protease inhibitor pepstatin. Antimicrob Agents Chemother 50(6): Singh N, Sijwali PS, Pandey KC and Rosenthal PJ. (2005) Plasmodium falciparum: Biochemical characterization of the cysteine protease falcipain-2'. Exp Parasitol 112(3): Pandey KC, Wang SX, Sijwali PS, Lau AL, McKerrow JH and Rosenthal PJ. (2005) The Plasmodium falciparum cysteine protease falcipain-2 captures its substrate, hemoglobin, via a unique motif. Proceedings of the National Academy of Sciences USA 102(26): *Sijwali PS, *Kato K, Seydel KB, Gut J, Lehman J, Klemba M, Goldberg DE., Miller LH and Rosenthal PJ. (2004) Plasmodium falciparum cysteine protease falcipain-1 is not essential in erythrocytic stage malaria parasites. Proceedings of the National Academy of Sciences USA 101(23): *Equal Contribution. 22. Na BK, Shenai BR, Sijwali PS, Choe Y, Pandey KC, Singh A, Craik CS and Rosenthal PJ. (2004) Identification and biochemical characterization of vivapains, cysteine proteases of the malaria parasite Plasmodium vivax. Biochemical Journal 378(2): Sijwali PS and Rosenthal PJ. (2004) Gene disruption confirms a critical role for the cysteine protease falcipain-2 in hemoglobin hydrolysis by Plasmodium falciparum. Proceedings of the National Academy of Sciences USA 101(13): Pandey KC, Sijwali PS, Singh A, Na BK and Rosenthal PJ. (2004) Independent intramolecular mediators of folding, activity, and inhibition for the Plasmodium falciparum cysteine protease falcipain-2. Journal of Biological Chemistry 279(5): Singh A, Shenai BR, Choe Y, Gut J, Sijwali PS, Craik CS and Rosenthal PJ. (2002) Critical role of amino acid 23 in mediating activity and specificity of vinckepain-2, a papain-family cysteine protease of rodent malaria parasites. Biochemical Journal 368(1): Rosenthal PJ, Sijwali PS, Singh A and Shenai BR. (2002) Cysteine proteases of malaria parasites: targets for chemotherapy. Current Pharmacology Design 8(18): (REVIEW). Puran S. Sijwali Page 3

4 27. Sijwali PS, Shenai BR and Rosenthal PJ. (2002), Folding of the Plasmodium falciparum cysteine protease falcipain-2 is mediated by a chaperone-like peptide and not the prodomain. Journal of Biological Chemistry 277(17): Sijwali PS, Shenai BR, Gut J, Singh A and Rosenthal PJ. (2001) Expression and characterization of the Plasmodium falciparum haemoglobinase falcipain-3. Biochemical Journal 360(2): Dua M, Raphael P, Sijwali PS, Rosenthal PJ and Hanspal M. (2001) Recombinant falcipain-2 cleaves erythrocyte membrane ankyrin and protein 4.1. Molecular Biochemical Parasitology 116(1): Sijwali PS, Brinen LS and Rosenthal PJ. (2001) Systematic optimization of expression and refolding of the Plasmodium falciparum cysteine protease falcipain-2. Protein Expression and Purification 22(1): *Shenai BR, *Sijwali PS, Singh A and Rosenthal PJ. (2000) Characterization of native and recombinant falcipain-2, a principal trophozoite cysteine protease and essential hemoglobinase of Plasmodium falciparum. Journal of Biological Chemistry 275(37): *Equal Contribution. 32. Sijwali PS, Malhotra P, Puri SK and Chauhan VS. (1997) Cloning and sequence analysis of the thrombospondin-related adhesive protein (TRAP) gene of Plasmodium cynomolgi bastianelli. Molecular Biochemical Parasitology 90(1): Sijwali PS and Lakhchaura BD. (1996) Detection and quantitation of Helicoverpa armigera nuclear polyhedrosis virus by ELISA technique. Indian Journal of Microbiology 36: Book chapter Sijwali PS. and Rosenthal PJ. (2017) The proteolytic repertoire of malaria parasites. D Gaur, CE Chitnis and VS Chauhan (Eds.), Advances in Malaria Research. John Wiley & Sons, Inc. 23 DEC DOI: / ch12 Commentary Invited by the Chemical & Engineering News to write a commentary on an article in the Nature journal. Title of the commentary: Hitting Malaria in the Proteasome. News of The Week Issue. 94(7): p 8. Print Date: February 15, 2016; Web Date: February 11, PRESENTATIONS Invited speaker, September 9-10, 2016: National conference on Malaria parasite biology: drug designing & vaccine development, Nirma University, Ahmedabad, India Invited speaker, January 27-Feb 6, 2015: Discovery of New Drugs against Malaria, ICGEB, New Delhi, India. Invited speaker, October 2014: Emerging fields in Biology & Biotechnology, Govt. Degree College, Srikakulam, India. Invited speaker, December 02-05, 2013: 82 nd annual meeting of the Society of Biological Chemists, India,University of Hyderabad, Hyderabad, India. Invited speaker, December 02-04, 2013: Recent Developments in Malaria Parasite Biology, ICGEB, New Delhi, India. Puran S. Sijwali Page 4

5 Poster, February 2012: Molecular Approaches to Malaria Lorne, Victoria, Australia. Invited speaker, December 2012: 36 th Social Science Congress: Social impact of malaria, KIIT University, Bhubaneswar, India. Invited instructor, December 2011: Refresher Course in Life Sciences, University of Hyderabad, Hyderabad, India. Invited speaker, October 2011: Emerging Trends in Microbial Interactions & Challenges in Human Health, Nirma University, Ahmedabad, Gujrat, India. Invited speaker, February 2010: Impact of Microbes on Health, Kuvempu University, Karnataka, India. Invited speaker, December 2010: Recent Developments in Malaria Research, ICGEB, New Delhi, India. Invited speaker and expert, November, 2008: Molecular Methods in Malaria Research, ICGEB, New Delhi, India. Poster, September 2009: Gordon Research Conference on malaria, Magdalen College, Oxford, UK. Speaker, September 2006: Molecular Parasitology Meeting XVI, Marine Biological Laboratory, Poster, February 2006: Keystone Symposium on Malaria: Functional Genomics to Biology to Medicine, Taos, New Mexico, USA. Poster, September 2004: Molecular Parasitology Meeting XIV, Marine Biological Laboratory, Poster, September 2003: Molecular Parasitology Meeting XIV, Marine Biological Laboratory, Poster, September 2002: Molecular Parasitology Meeting XIII. September 2002, Marine Biological Laboratory Speaker, September 2001: Molecular Parasitology Meeting XII, Marine Biological Laboratory, Speaker, September 2000: Molecular Parasitology Meeting XI, Marine Biological Laboratory, Poster, November 1998: 10 th International Congress of Immunology, New Delhi, India. Poster, August 1997: 2 nd Global Meet on Parasitic Diseases, Hyderabad, India. Poster, March 1996: Global Meet on Parasitic Diseases, New Delhi, India. Funds Received Sl. No. Title of Project Funding Amount Duration Agency (lakhs) 1 Structure-Activity Relationship and DBT /01/2010 to Biological Roles of Falcipain-3, an 31/07/2013 Anti-Malarial Drug Target 2 Cysteine Proteases of Malaria Parasites DBT 31.8 as Drug Targets: Identification of 17/06/2010 to Essential and Functionally Conserved 31/12/2013 Proteases 3 Biological roles and therapeutic DST /06/2013 to potential of a conserved Plasmodium 19/06/2016 DJ1-PfpI superfamily protein 4 Roles of Plasmodium protein CSIR /01/2012 to Puran S. Sijwali Page 5

6 degradation machineries in parasite development and pathogenesis of malaria 5 Molecular Machinery of Plasmodium: Signaling, Autophagy and UPS 6 Investigation of Autophagy in Malaria Parasites DBT (COE) DBT 31/03/ /11/2015 to 5/11/2020) /12/2016 to 4/12/2019 Puran S. Sijwali Page 6