STUDY THE EFFECT OF PURIFIED CATECHINS OF GREEN TEA ON THE MOST IMPORTANT CAUSES OF BACTERIAL INFECTIONS OF THE URINARY TRACT AND SYNERGISTIC EFFECT

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1 * * - - *, API Ecoli (%) Kpneumoniae (%) Ecoli Kpneumoniae Catechin / () / (100,75,,) (Catechin) Kpneumoniae Ecoli Gentamycin Gentamycin (Catechin) Kpneumoniae STUDY THE EFFECT OF PURIFIED CATECHINS OF GREEN TEA ON THE MOST IMPORTANT CAUSES OF BACTERIAL INFECTIONS OF THE URINARY TRACT AND SYNERGISTIC EFFECT ESSAM FAAl-jumaily, *Yasir AJAl-Abdli,*Nazar ENasser Department of Biotechnology, Institute of Genetic Engineering and Biotechnologyfor post graduate Studies, University of Baghdad Baghdad- Iraq *Department of Biology, College of Science, Unversity of Al-Mustansirya Baghdad - Iraq Abstract Two hundred urine samples were collected from patients infected with acute and chronic urinary tract infection, 85 bacterial isolate were isolated from these samples and were diagnosed using biochemical tests and kit API test, most of them identified Ecoli (235%) Kpneumoniae rate (176%) Isolates were selected Ecoli and Kpneumoniae according to their isolation rate from urinary tract infection cases and their antibiotic resistance The inhibition effect of the purified catechin from the green tea was studied when the isolates were grown in the following concentrations (25, 50,75, 100) mg/ml The concentration of 100 mg/ml showed a high action against the two species The combination effect of the purified catechin was estimated with both and Gentamycin on the resistant isolates to these antibiotics (Ecoli and Kpneumoniae) and has been compared to the minimum ٤

2 inhibitor concentration of the isolates before and after the confusion of antioxidants, as the effect of mixing and Gentamycin with Catechin, it gave a synergic effect with on two isolates, either Gentamycin gave synergic effect on Kpneumoniae Key words: Purified catechins, Green tea, Bacterial infections Ecoli) و (Kpneumoniae / (Two fold dilution method) MIC s Muller-Hinton agar [9] Ridgway Stocks : / (Amikacim Gentamycin) [] Jin [1] Ecoli (%-) [2,3] [4] Kpneumoniae (multi microbial infection) [5] (antibacterial activity) [6] وepigallocatechin وepicatechin catechin) وgallate epicatechin وgallate (epigallocatechin Epigallocatechin gallate (EGCg) methicillin-resistant Staphylococcus aureus [7] (MRSA) (EGCg) Minocycline and Ofloxacin (Indifference) (Addition) [8] (EGCg) ٥

3 spp [12,13] Klebsiella [12,13] Ecoli Kpneumoniae / () [14] Willson, ;[15] Piskula and Terao Intestinal normal micro flora Yee [] Takabayashi [] Hpylori Clostridium Bacillus Salmonella (Catechin) : ( / ) ( ) E coli Kpneumoniae (Catechin) Catechin () () Gentamycin Ecoli / () () Gentamycin K pneumoniae / Lyophilizer : Checker Board Assay Muller-Hinton agar (: : : :) (Fractional inhibitory concentration) MIC MIC : = -< -< < [11] > Ecoli ( ) ٦

4 512 Kpneumonia e *: Fractional lnhibitory Concentation () () [19] [20] (Pencillin binding proteins) (PBPs) [] Gradisar DNA [8] ATP gyrase 1 Raksha,R; Srinivasa, H,and Macaden,RS2003Occarrenceandcharacterisatio nofuropathogenic Escherichia coli in Urinary trach infection Indian J Med Microbiol 2: Todar, K 2002 Pathogenic E coli J of Becteriology,33: Nester, EW; Anderson, DG; Roberts, CE, Jr; Pearsall, NN and Nester, MT 2001 Microbiology A Human Perspective (3 rd ed) McGraw- Hill Higher Education,New York 4 Ebringer,A;Rashid,T;Wilson,C;Ptaszy nska,t&markfielder,m2006ankylosi ng Spondylitis, HLA-B27 and Klebsiella An Overview: Proposal for early diagnosis and treatment Current Rheumatology Reviews2: Iwalokun, B;Olukosi, Y;Adejoro, A;Olaye, J; and Fashade, O2004 Comparative biochemical and molecular evaluation of Swarming of proteus and () MIC (Catechin) K E coli / [18] Mandal pneumoniae () (Catechin) Gentamycin ( / 1024 ) / : () MIC () Ecoli () Kpneumoniae Gentamycin Kpneumoniae (Catechin) Ecoli () : Index MIC E coli Kpneumoni ae *: Fractional lnhibitory Concentation : لاضافة Index µg/m l MIC 256 E coli ٧

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