LIST OF TESTS WITH COLLECTION INFORMATION. Form Page 1 of 19

Transcription

1 ALL TESTS Tests listed are the most commonly ordered tests. For further information on tests not listed call the laboratory, look for the test in the computer or look at Lab Corp Manual ACTH (Adenocorticotropic Hormone) (Cortrosyn) AFB culture and smear (TB culture) Activated Protein C Resistance (APCR) Plasma Ice Plastic Lavender-top (EDTA) prior to collection Label Blood Bank specimens with: full name, MR#, V#, DOB, date, time, collector s initials. Other specimens: Full name & ID# or DOB (2 pt-identifiers), date, and time. Iced Plastic Lavender-top; immediately immerse in ice bath after collection Note the Exact Time of Collection Varies Specimen dependent Refrigerate specimens. Specimen dependent; first morning sputum; fasting gastric aspirate 2 ml frozen plasma Blue-top tube Do not draw from arm with Should not be used for patients receiving thrombin inhibitors such as hirudin and argatroban. Improperly labeled, improper collection container or preservative, gross physical contamination of specimen container, gross hemolysis, insufficient specimen quantity, time or storage limits exceeded, improper patient preparation, IV contamination, etc. See Policy QP 100 Criteria for Rejection of Specimens Not received in Lab in ice bath. Collected in nonsiliconized glass tube Same as All Tests above Hemolysis; thawed specimen; clotted specimen; specimen not in 3.2% citrate. One hour for tests on list (see test list) If test performed at AMC Labs: Daily 8 hour TAT If Reference Lab may take 48 or more. Reference Lab: Smears Culture up to 6 weeks. Albumin Serum, 0.5 ml. Gold or Red-top or Green Same as All Tests above Same as All Tests above Daily, 8 Aldolase Serum or plasma, 2 ml Gold, Red, Blue, Green, or Same as All Tests above Same as All Tests above Reference Lab= Lavender-top 48 Aldosterone Level Serum, 1 ml, Plasma Gold, Red or Lavender-top Same as All Tests above Same as All Tests above Reference Lab= 48 Aldosterone, 24-hr. Urine Urine, 10 ml aliquot 4 oz. urine container 24 hour urine collection Boric acid preservative not used, ph<2, recently administered isotopes Aldosterone & Renin Activity 1 ml Serum and 1 ml Plasma 1 red-top and 1 Lavender-top (EDTA) Send to Lab ASAP as plasma must be frozen Patients should ambulate if able for at least 30 minutes prior to collection. Note whether the patient is upright or supine when the specimen is collected. Same as All Tests above Alkaline Phosphatase, Blood Serum, 0.5 ml Gold or Red-top or Green Same as All Tests above Same as All Tests above Daily, 8 Alkaline Phosphatase, Heat Fractionated Alpha-1-antitrypsin Alpha Feto Protein Maternal Serum Serum, 2 ml. Red, Green or Gel Same as All Tests above Same as All Tests above Daily, 8 Serum (preferred) or plasma, 1mL Gold or Red (preferred) or Green-top or Lavender-top Same as All Tests above Overnight fasting preferred Chylous serum Serum, 3 ml Gold-top or Red-top Same as All Tests above Same as All Tests above Form Page 1 of 19

2 Alpha Feto Protein tumor Marker ALT. (SGPT) Alanine Transaminase, Blood Aluminum Serum, 3 ml Gold or Red-top Same as All Tests above Same as All Tests above Serum, 0.5 ml. Gold or Red-top or Green Same as All Tests above Same as All Tests above Daily, 8 Whole blood, serum or plasma Royal Blue-top (Sodium Heparin or EDTA) or Lavender (EDTA) or Redtop No gel tubes Send unopened royal blue or lavender tube. Red-top must be spun and separated within 45 minutes. Gel tube; unspun red-top Amebiasis antibodies Serum, 1 ml Red or Gold top Same as All tests above Same as All tests above Amino Acids, quant. 24-hr urine 10 ml aliquot 24 hr. urine container, no preservative patient s age & sex No preservatives Instructions for 24-hour urine collection Fecal contamination of urine Ammonia Plasma, 2-3 ml. Green-top Collect on ice Not received on ice Daily, 8 on ICE Amylase, Blood Serum, 0.1 ml Gold or Red-top or Green Same as All Tests above Same as All Tests above Daily, 8 LP - Gold or Red-top Amylase, Fluid Body fluid Fluid in green top tube Same as All Tests above Same as All Tests above Daily, 8 Amylase, isoenzymes Serum, 2 ml Gold or Red-top Same as All Tests above Same as All Tests above Amylase, Urine Urine, 10 ml 4-oz specimen collection Same as All Tests above Same as All Tests above Daily, 8 container Amylase, 2-hr Urine Urine, 10 ml. 4-oz specimen collection Same as All Tests above Have patient urinate; start Specimen not timed Daily, 8 container 2-hour period from this point on. correctly. Androstenedione Serum, 1 ml Red or Lavender (EDTA) Same as All Tests above No isotopes 24 before Venipuncture Angiotensin I Conv. Enzyme Serum, 2 ml Gold or Red-top Same as All Tests above Stop captopril, enalapril or lisinopril for 12 prior to venipuncture Whole blood, hemolysis, icterus. Anticytoplasmic Neutrophil Serum, 1 ml Gold or Red-top Same as All Tests above Same as All tests above; Antibody (ANCA) gross lipemia. Antidiuretic Hormone (ADH; Arginine Vasopressin; Vasopressin) Antimicrosomal Thyroid Ab (TPO) 1 ml serum & 2 ml plasma Gold-top or Red-top AND Lavender-top (EDTA) Send tubes to Lab ASAP No isotopes 24 prior to Venipuncture Same as All tests above 1 ml serum Gold-top or Red-top Same as All Tests above Same as All Tests above Form Page 2 of 19

3 Anti-platelet Antibody (qual) 0.5 ml serum Gold-top or Red-top Same as All tests above Same as All Tests above Antithyroglobulin Antibody 1 ml serum Gold-top or Red-top Same as All tests above Hemolysis Antithyroid antibodies (ATA) 1 ml serum Gold-top or Red-top Includes: Antimicrosomal and anti-thyroglobulin Antithrombin III Activity 2 ml frozen plasma Blue-top tube Do not draw from arm with (Functional) Antithrombin III Antigen (Immunologic) Antithrombin III Panel (Antigen and Activity) 2 ml frozen plasma Blue-top tube Do not draw from arm with 3 ml frozen plasma Blue-top tube Do not draw from arm with Same as All Tests above Should not be performed on Hemolysis; thawed specimen; patients receiving heparin clotted specimen therapy. Warfarin therapy can cause false normals; Thrombin inhibitors such as hirudin and argotroban can interfere with analysis. Should not be performed on patients receiving heparin therapy. Warfarin therapy can cause false normals; Thrombin inhibitors such as hirudin and argotroban can interfere with analysis. Should not be performed on patients receiving heparin therapy. Warfarin therapy can cause false normals; Thrombin inhibitors such as hirudin and argotroban can interfere with analysis. Arsenic, Blood 2 ml whole blood Royal Blue (EDTA) Same as All Tests above Patient must avoid all seafood for 72 prior to collection Arsenic, Urine 5 ml urine, Random or 24 hr. aliquot Urine cup or 24-hr urine Same as All Tests above Patient must avoid all seafood for 72 prior to collection Hemolysis; thawed specimen; clotted specimen Hemolysis; thawed specimen; clotted specimen Clotted specimen Same as All Tests above AST, (SGOT) Aspartate Serum, 0.5 ml. Gold or Red-top or Green Same as All Tests above Same as All Tests above Daily, 8 Transaminase, Blood Barbiturates 3 ml serum or plasma Red, lavender (EDTA) or Green. Same as All Tests above Gel tubes No gel tubes Basic Metabolic Profile Serum or Plasma, 1 ml. Gold, Red or Green-top Same as All Tests above Same as All Tests above Daily, 8 Bence Jones Protein see Immunofixation and Protein Electrophoresis, Urine B-2 Glycoprotein 1 Ab 2 ml Serum Gold or Red top Same as All Tests above Same as All Tests above Beta Hydroxybuturate 0.1 ml Serum or Plasma Red, Green or Gel Same as All Tests above Tube opened for extended time Daily, 8 Form Page 3 of 19

4 Beta Sub Unit Serum. 1.0 ml. Gold, Red or Green-top Same as All Tests above Same as All Tests above Daily, 8 Bicarbonate Serum, 1.0 ml. Gold, Red or Green-top Same as All Tests above Same as All Tests above Daily, 8 Bilirubin, Total & Direct Serum 0.5 ml. Gold, Red or Green-top Same as All Tests above Same as All Tests above Daily, 8 Blood Banking Procedures in Separate Section of this Manual. B-type Natriuretic Peptide Plasma Lavender Same as listed for All Tests Same as All Tests above Daily, 8 B12 (alone) or B12 & Folate Serum or Plasma Red, Green or Gel Same as All Tests above Gross Hemolysis Daily, 8 BUN (Blood Urea Nitrogen) Serum or Plasma Gold, Red or Green (Lithium Same as All Tests above Same as All Tests above Daily, 8 heparin) Borrelia bergdorferi see Lyme Disease testing CA 15-3 Tumor antigen 0.8 ml Serum Gold or Red-top Same as All Tests above Same as All Tests above CA Tumor antigen 1 ml Serum Gold or Red-top Same as All Tests above Same as All Tests above CA 125 Tumor antigen 0.8 ml Serum Gold or Red-top Same as All Tests above Same as All Tests above Cadmium, Blood 7 ml Whole Blood Royal Blue (EDTA) Same as listed for All Tests Clotted specimen Cadmium, Urine 5 ml Urine, random or 24 hr. aliquot Urine cup or 24 hr. Same as listed for All Tests Same as listed for All Tests Calcium, Blood Serum, l.0 ml. Gold, Red or Green (Lithium Same as listed for All Tests Same as listed for All Tests Daily, 8 heparin) Calcium, Urine (24 hour) Urine 10 ml. 24-hr Urine container with Same as listed for All Tests Same as listed for All Tests Daily, 8 HCl. (May also be collected without preservative, but must be acidified prior to testing) Cannabinoids (THC) Urine or Blood Urine: random urine cup Blood: Gray top tube Chain of custody documentation required for employment or forensic Same as All tests above purposes. Carbon Dioxide, Total Serum, 1.0 ml. Gold, Red or Green-top Same as listed for All tests Same as All Tests above Daily, 8 Carboxyhemoglobin (CO; Carbon monoxide) Whole blood Green-top tube (Lithium heparin) Same as All Tests above Specimen other than green top tube Daily, 8 Form Page 4 of 19

5 Cardiolipin antibodies IgG and/or IgM and/or IgA 1 ml Serum Gold or Red-top Same as All Tests above Same as All Tests above CEA Serum, 0.8 ml Gold or Red-top Same as All Tests above Same as All Tests above Celiac Disease Profile Serum, 3 ml Gold or Red-top Includes: Gliadin Ab IgG/IgA; t-transglutaminase IgG/IgA; Endomysial Ab IgA; Total IgA Same as above; lipemia Ceruloplasmin Serum, 1 ml preferred or plasma Gold (preferred) or Red-top or Green top Same as All Tests above Room temp storage Chloride, Blood Serum or Plasma, 1 ml. Gold, Red or Green-top Same as All Tests above Same as All Tests above Daily, 8 Chloride, 24 hour Urine Urine 10 ml aliquot 24 hr. container, no See lab instruction sheet on All urine excreted in a 24- Daily, 8 preservative 24-hour urine collections hour period is not collected Chloride, Random Urine Urine 4-oz urine container Spot urine (same as for Same as All Tests above Daily, 8 urinalysis) Chemistry Profile (ER) Serum or Plasma, 1 ml. Gold, Red or Green-top Same as listed for All tests Same as All Tests above Daily, 8 Cholesterol Serum or Plasma Gold, Red or Green-top Same as listed for All tests Same as All Tests above Daily, 8 Chlamydia GC DNA Nucleic Acid amplification Swab from vaginal/cervical area or Obtain container from laboratory Physician does collection when examining patient Males must not urinate for one hour prior to collection Same as All Tests above Daily, 2, or urine Chlamydia GC DNA Nucleic Acid Amplification Other sources: ocular, pharyngeal, rectal Obtain APTIMA Collection kit from laboratory Physician does collection when examining patient Same as All Tests above Clean-Catch Midstream Urine Urine, first morning void Clean-catch kit or sterile. Pt. should follow clean-catch Use wash pack and clean Specimen unpreserved and Daily, 8 Specimen (Urinalysis) or catheterized spec. container with wash pack procedure area thoroughly unrefrigerated>2 Complete Blood Count (CBC) Whole Blood Same as All Tests Same as All Tests Daily, 8 Clonazepam level Serum, 4 ml or plasma Red-top tube; No gel tubes, Lavender or Green Same as listed for All tests Gel tube Clozapine (Clozaril) level Serum, 1 ml Red-top or Lavender or Green (No gel tubes) Same as listed for All tests Gel tube CMV IgG or IgM Serum, 0.5 ml Gold or Red-top tube Same as listed for All tests Hemolysis; lipemia Cocaine Metabolite Urine, 30 cc 4 oz. Urine container Same as above; follow chain of custody documentation for Same as listed for All tests employee testing and forensic purposes Complement C3 Serum 1 ml (preferred) or plasma Gold or Red (preferred) or Green-top Submit ASAP Lipemia Complement C4 Serum 1 ml (preferred) or plasma Gold or Red (preferred) or Green-top or Lavender-top Submit ASAP Lipemia Form Page 5 of 19

6 Comprehensive Metabolic Serum, 2 mils. Gold, Red or Green-top Same as All Tests above Same as listed for All tests Daily, 8 Chemistry Profile (CMP) C-Peptide 0.8 ml serum Gold or Red-top tube Same as All Tests above Patient must be fasting Patient not fasting C-Reactive Protein (CRP) Serum Red-top tube Same as All Tests above Same as All Tests above Daily, 8 C-Reactive Protein (CRP), high sensitivity, cardiac 1 ml serum or plasma Gold, Red or Green-top or Lavender-top Creatinine Serum or Plasma Gold, Red or Green-top Creatinine, Random Urine Urine 4 oz. screw top urine container Creatinine, 12 hour Urine Urine 4 oz. screw-top Urine container Creatinine, 24 hour Urine 10 ml aliquot of urine 24 hr. urine container, no preservatives Creatinine Clearance Serum AND 24 hr. urine or plasma Gold, Green, Red-top tube and 24 hr. urine container, no preservatives Creatinine Clearance, Estimated Creatine Kinase, Blood, (CK) Serum or Plasma Gold, Red or Green-top Same as All Tests above Same as all tests ; lipemia Same as listed for All tests Same as All Tests above Daily, 8 Spot urine collection, (same as for urinalysis) Collect over 12 hour time period Follow collection protocol for 24-hour urines Collect blood specimen within 24 of the urine collection Same as listed for All tests Daily, 8 Same as listed for All tests Daily, 8 Collection procedure must be explained & an information sheet supplied to patient Not all urine passed was collected in the 24-hour time period Daily, 8 24 hr. urine instructions Same as All Tests Daily, 8 Serum Red-top tube Venipuncture Same as All Tests Daily, 8 Same as All Tests above Same as listed for All tests Daily, 8 Cortisol Serum, 0.8 ml. Gold or Red-top tube Same as listed for All tests, unless timed test, times must be recorded Plasma h Form Page 6 of 19

7 Cyanide level Whole blood, 1 ml Gray (sodium fluoride) Submit unopened tubes Same as All tests above Cyclosporine Whole Blood, 2 ml Lavender-top (EDTA) or Green-top Same as all tests above Same as All tests above Cystic fibrosis Whole blood, 7 ml 2 lavender-top tubes (EDTA) Submit signed informed consent form and family history of known mutations Cytogenetics Peripheral Blood D-dimer Plasma, 2 ml Blue-top tube Draw waste tube before specimen tube if using butterfly. DHEA or DHEAS 0.3 ml Serum or plasma Gold or Red-top or Same as all tests above (Dehydroepiandrosterone) Digoxin Serum Gold, Red-top tube or Green top Dilantin (Phenytoin) Serum or plasma Red-top tube or Green top No gel tubes Same as above; frozen specimen Reference Lab= 1 week Whole blood, 5 10 ml Green, kit available Same as all tests above Same as All Tests above Reference Lab 10 day Same as All Tests above Daily, 8 No radioisotopes prior to Gross lipemia; recently test administered isotopes 6 to 8 after dose Same as all tests above Daily, 8 Same as all tests above SST Daily, 8 Drug Screen, qualitative Urine, 30 ml 4 oz. urine container Same as all tests above Same as all tests above Daily, 8 EBV Epstein-Barr Virus See below for specific Testing tests E2 - Estradiol Serum or Plasma Red, Green or Gel Same as all tests above Same as all tests above Daily, 8 EBV Early Antigen Ab IgG 1 ml serum Gold or Red-top tube Same as all tests above Hemolysis, lipemia EBV Nuclear Antigen Ab IgG 1 ml serum Gold or Red-top tube Same as all tests above Hemolysis, lipemia EBV Viral Capsid Antigen Ab 1 ml serum Gold or Red-top tube Same as all tests above Hemolysis, lipemia IgG EBV Viral Capsid Antigen Ab 1 ml serum Gold or Red-top tube Same as all tests above Hemolysis, lipemia IgM EBV Chronic/Active Infection Panel 2 ml serum Gold or Red-top tube Includes Early Antigen IgG, Nuclear Antigen IgG, and Viral Capsid Antigen IgG EBV Acute Infection Panel 2 ml serum Gold or Red-top tube Test includes Early Antigen IgG, Nuclear Antigen IgG, Viral Capsid Antigen IgG, Viral Capsid Antigen IgM EBV DNA by PCR 2 ml plasma 1-2 lavender-top tubes (EDTA) Quantitative range: 100-1,000,000 copies/ml Hemolysis, lipemia Hemolysis, lipemia Same as all tests above Form Page 7 of 19

8 Electrolytes (Na, K, Cl, Serum or Plasma Gold, Red or Green-top Same as all tests above Same as all tests above Daily, 8 TCO2) Eosinophil Count Whole Blood 1 lavender-top tube (EDTA) Same as all tests above Same as all tests above Daily, 8 ESR (Erythrocyte Whole Blood Same as all tests above Same as all tests above Daily, 8 Test Sedimentation Rate) requires l hour Estradiol (E2) Serum Gold-top or Red top Same as all tests above Same as all tests above Daily, 8 Estrogen, Total Serum or plasma Gold, Red or Green-top Ethylene Glycol Serum or plasma 7 ml Gold-top or Green-top ETOH (Ethanol) Serum or plasma Unopened Gold, Red, or Green-top tube Same as all tests above No radioisotopes prior to test Same as all tests above Submit tube unopened Same as all tests above Venipuncture without Same as All Tests above Daily, 8 or alcohol use povidone-iodine capabilities or soap and water Factor V Leiden Mutation 7 ml Whole Blood 2 Same as All Tests above Hemolysis; frozen specimen Factor II Prothrombin DNA (mutation) 7 ml Whole Blood 2 Same as All Tests above Hemolysis; frozen specimen Ferritin Serum Gold, Red or Green-top Same as all tests above Same as listed for All tests Daily, 8 Fibrinogen Plasma Blue-top tube Draw waste tube before Same as listed for All tests Daily, 8 specimen tube if using butterfly. FISH/Target Gene Whole blood, 5-10 ml Green, kit available Same as all tests above Same as listed for All tests Reference Lab 10 days Flow Cytometric Analysis Peripheral Blood 5-10 ml whole blood; collect partner CBC, Green or Yellow; kit available Same as all tests above Same as All Tests above w/smear for path review Folic Acid Serum Gold or Red-top tube Same as all tests above Same as All Tests above Follicle Stimulating Hormone Serum or Plasma Red, Green or Gel Same as all tests above Same as all tests above Daily, 8 (FSH) Free T3 1 ml serum or plasma Gold, Red, Green top tube Same as all tests above Same as All Tests above Daily, 8 Free T4 0.5 ml serum or plasma Gold, Red, Green top tube Same as all tests above Same as All Tests above Daily, 8 Free Kappa / Lambda Light Chains, Serum Free Kappa / Lambda Light Chains, Urine Gastric Poly Count 0.5 ml Serum Gold or Red-top tube Same as all tests above Same as all tests above 0.5 ml Urine, random or Urine cup or 24 hr. Same as all tests above Same as all tests above 24 hour aliquot Gastric aspirate-amount Syringe Physician/PA performs Insufficient quantity Daily, 8 or obtained capabilities Form Page 8 of 19

9 GC DNA Probe Genprobe swab only Gen Probe Tube obtained from Physician/PA obtains swab during examination; maintain swab at room temp. Males must not urinate for one hour prior to collection More than one swab in tube; specimen frozen Daily, 2 or Gentamicin Peak or Trough Serum or Plasma Gold, Red or Green-top Geional Diabetic Screen Serum or Plasma Gold, Red or Green-top GGT (Gamma Glutamyl Transpeptidase), Blood Glucose Tolerance Test (GTT) Geional Serum or Plasma Serum or Plasma 1 ml. Gold, Red or Green-top Gold, Red or Green-top Glucose (Random/Fasting) Serum or Plasma-1 ml. Gold, Red or Green-top Gram Stain 1 swab, 1 slide or 1 ml of Sterile containers for fluids, specimen culturette, needle aspiration, or inoculated slide Haldol 4 ml Serum or plasma; Red or Green-top or No gel tubes Lavender-top Haptoglobin 2 ml Serum (preferred) Gold, Red, Green-top or or plasma Trough: Collect 10 minutes before dose Peak: Collect 30 minutes after infusion Same as all tests above Same as All Tests above Daily, 8 or Patient receives 50 grams glucose, blood collected in 1 hour. Same as All Tests above Daily, 8 Same as all tests above Same as listed for All tests Daily, 8 Same as all tests above Same as all tests above Specimen collected to avoid contamination with skin, & non-sterile surface Patient fasts overnight; remains seated; no smoking throughout the test If fasting specimen pt. must be fasting Same as listed for All tests Patient not fasting for FBG Specimen not collected in appropriate container and All Tests above. Daily, 8 Daily, 8 Daily, 8 or Same as all tests above Use of SST Avoid traumatic Avoid exercise prior to Hemolysis due to traumatic venipuncture collection venipuncture Heavy Metals Profile I, Blood 7 ml Whole Blood Royal Blue (EDTA) Tests for Arsenic, Lead, and Avoid seafood and red wine Clotted specimen Mercury 72 prior to collection Heavy Metals Profile I, Urine 15 ml Urine, Random or Urine cup or 24 hr. container Tests for Arsenic, Lead, and Avoid seafood and red wine Same as All Tests 24 hr. aliquot (no preservative) Mercury 72 prior to collection Heavy Metals Profile II, Blood 7 ml Whole Blood Royal Blue (EDTA) Tests for Arsenic, Lead, Avoid seafood and red wine Clotted specimen Mercury, and Cadmium 72 prior to collection Heavy Metals Profile II, Urine 15 ml Urine, Random or Urine cup or 24 hr. container Tests for Arsenic, Lead, Avoid seafood and red wine Same as All Tests 24 hr. aliquot (no preservative) Mercury, and Cadmium 72 prior to collection Helicobacter pylori Screen Serum, plasma Gold, Green, Blue, Red-top Same as All Tests above Same as All Tests Daily, 8 tube Helicobacter pylori Titer Serum 2 ml Gold or Red-top tube Same as All Tests above Same as All Tests Hemochromatosis Evaluation Whole Blood, 7 ml 2 lavender-top tubes (EDTA) Same as All Tests above Frozen specimen Hemoglobin, glycated (A1C) Plasma 3 ml. 1 lavender-top tube (EDTA) Same as All Tests above Same as All Tests above Daily, 8 Hemoglobin and/or Whole blood 1 lavender-top tube (EDTA) Same as All Tests above Same as All Tests above Daily, 8 or Hematocrit HDL Cholesterol Serum or plasma Gold or Red-top tube or Same as All Tests above Patient must be fasting Patient not fasting Daily, 8 Green-top tube Form Page 9 of 19

10 HEPATITIS TESTING REFER TO SPECIFIC TESTS LISTED BELOW Hepatitis A IgM or Total Serum or plasma, 1 ml Gold or Red-top tube or Hepatitis B Surface Antibody 1 ml serum or plasma Gold or Red-top tube or Hepatitis B Surface Antigen 3.5 ml serum or plasma Gold or Red-top tube or Hepatitis B Core IgM or Total Serum or plasma, 1 ml Gold or Red-top tube or Hepatitis B Quantitation 3 ml serum or plasma Gold or Red-top tube or (by PCR) Same as All Tests above Same as All tests above Same as All Tests above Same as All Tests above Same as All Tests above Same as All Tests above Same as All Tests above Same as All tests above Used for known HBV pos patients. Quantitative range: 29 IU/mL -110,000,000 IU/mL; ( ,200,000 copies/ml) Hepatitis B NGI Superquant (by PCR) 1 ml serum or plasma Gold or Red-top tube or Used for known HBV pos patients. Quantitative range: 100-5,000,000 copies/ml Hepatitis B NGI Ultraqualitative (by PCR) 2.5 ml serum or plasma Gold or Red-top tube or Hepatitis Be Antibody 1 ml serum or plasma Gold or Red-top tube or Hepatitis Be Antigen 1.5 ml serum or plasma Gold or Red-top tube or Hepatitis B Genotype 2.5 ml serum or plasma Gold or Red-top tube or Hepatitis C Ab Serum or Plasma, 2 ml Gold or Red-top tube or Qualitative sensitivity of 1-2 copies/ml Same as All Tests above Same as All Tests above Same as All Tests above Same as All Tests above Test on patients known to be HBV positive Same as All Tests above Same as All tests above Form Page 10 of 19

11 Hepatitis C RT-PCR Quantitation Hepatitis C NGI Quantasure (by PCR) 3.5 ml serum or plasma Gold or Red-top tube or 3.5 ml Serum or plasma Gold or Red-top tube or Hepatitis C NGI Superquant 3.5 ml serum or plasma Gold or Red-top tube or Hepatitis C NGI Ultraqualitative 2.5 ml serum or plasma Gold or Red-top tube or Hepatitis C Genotype 3.0 ml serum or plasma Gold or Red-top tube or Used for known HCV positive patients. Quantitative range: 43 IU/mL -69,000,000 IU/mL Used for known HCV positive patients. Quantitative range: 2 IU/mL -2,000,000 IU/mL (5-5,000,000 copies/ml) Used for known HCV positive patients. Quantitative range: 39 IU/mL -2,000,000 IU/mL (100-5,000,000 copies/ml) Qualitative sensitivity: 2-3 IU/mL (5-8 copies/ml) Test may fail if patient s viral load is <600 copies/ml. Viral load will be performed to confirm samples that fail genotype. Hepatitis Panel Acute 4 ml Serum or Plasma Gold or Red-top tube or HERPES SIMPLEX Refer to specific tests TESTING below Herpes Simplex 1 and 2 IgG 1 ml serum Gold or Red-Top No distinction between types antibodies, total 1 and 2. Herpes Simplex 1 and 2 IgG 2 ml serum Gold or Red-Top Reflexes to type specific if antibodies with reflex to type positive. specific if positive Herpes Simplex 1 and 2 IgG type specific antibodies Herpes Simplex type 1 IgG antibodies Herpes Simplex type 2 IgG antibodies Same as All Tests above Same as All Tests above Hemolysis; lipemia Hemolysis; lipemia 1.5 ml serum Gold or Red-Top Tests for type specific 1 and 2 IgG. Same as All Tests above 1 ml serum Gold or Red-Top Type 1 only Same as All Tests above 1 ml serum Gold or Red-Top Type 2 only Same as All Tests above Form Page 11 of 19

12 Herpes Simplex 1 and 2 IgM antibodies, total Herpes Simplex 1 and 2 IgM specific antibodies Herpes Simplex 1/2 by PCR Herpes Simplex culture and typing 2 ml serum Gold or Red-Top No distinction between types 1 and 2. 1 ml serum Gold or Red-Top Tests for type specific 1 and 2 IgM. CSF, swab, whole blood, serum or plasma Vesicular fluid, ulcerated lesions, pharyngeal and throat swabs, urine, CSF, autopsy and biopsy material, eye exudates, vaginal swabs. Sterile container, swab, Gold or Red-top tube or Lavendertop tube (EDTA) Swabs in viral transport medium, 1 ml fluid, 0.5 grams of tissue in viral transport medium. Do not freeze whole blood. Other specimens may be room temperature, refrigerated, or frozen Deliver to lab ASAP; always refrigerate. HIV - Routine Serum Gold or Red-Top Informed consent form must be signed by HCP; patient s name on tube must exactly match name on form (same order of first and last names, etc.). Hemolysis; lipemia Hemolysis; lipemia Same as All Tests above Per type of specimen Patient must be counseled and must give informed consent. Dry specimen; wooden shaft swab; room temperature storage or transport; Informed consent not received; name mismatch between form and tube. HIV expedited (SUDS; EHT) Whole blood or capillary blood, Green (Na heparin), Blue Same as All Tests above HIV-1 RNA by PCR 3.5 ml Plasma Assay range: 48-10,000,000 copies/ml If testing newborn whose mother refused, informed consent is not required; if testing source patient of employee exposure, informed consent must be obtained. Required informed consent not documented. heparinized plasma Daily, performed. TAT=2. Form Page 12 of 19

13 HIV Genotype 5 ml Plasma 2 Lavender-top tubes (EDTA) May not be successful if viral load is <1000 copies/ml. If specimen fails Genotyping, HIV-1 RNA quantitation will be performed to confirm viral load. HLA B27 2 ml Whole blood Same as All Tests above Hemolysis; clotted specimen; non-edta tube Homocysteine Plasma (preferred) or Same as All Tests above Same as All Tests above; serum or Red or Green-top lipemia Immunofixation (IFE), Serum 2 ml Serum Gold or Red-Top Includes the quantitation of immunoglobulins IgG, IgM, IgA Immunofixation (IFE), Urine Immunofixation and Protein Electrophoresis Panel, Serum (IFE + PE) Immunofixation and Protein Electrophoresis, Urine (IFE + PE) Immunofixation and Protein Electrophoresis Panel, CSF (IFE + PE) Immunoglobulins IgG, IgM, IgA 10 ml Urine, Random or 24 hour aliquot Urine cup or 24 hr. 2 ml serum Gold or Red-Top Includes the quantitation of immunoglobulins IgG, IgM, IgA 30 ml Urine, Random or 24 hour aliquot 5 ml (minimum 2 ml) of CSF Urine cup or 24 hr. Patient should be fasting Hemolysis; lipemia Same as All Tests above unrefrigerated specimen; preservative used. Patient should be fasting Hemolysis, lipemia Same as All Tests above unrefrigerated specimen; preservative used. sterile container Same as All Tests above Bloody specimen; <2 ml Serum, 3 ml Gold or Red-Top Same as All Tests above Patient should be fasting Lipemia Influenza A and/or B Ab Serum Gold or Red-Top Same as All Tests above Same as All Tests above Insulin Level Serum Gold or Red-Top Same as All Tests above Hemolysis; plasma specimen Ionized Calcium Serum Gold-top tube (gel) Must send spun, unopened tube. Hemolysis, gel tube opened; gel barrier not completely sealing the clot. Iron (Fe) Serum or plasma Gold, Red or Green-top Send to lab ASAP Same as All Tests above Daily, 8 Iron Binding Capacity (IBC, Serum or plasma Gold, Red or Green-top Send to lab ASAP Same as All Tests above Daily, 8 TIBC, Transferrin Saturation; Iron Saturation) La (anti-la; SSB) see Sjogren s antibodies Lactose Tolerance Test Timed Test Fasting Call laboratory for protocol Fasting, & several timed tests Lactose soln. rec d from lab Timing and Lactose prep. Daily, 8 OP has to be administered not done correctly LD (Lactose Dehydrogenase; LDH) Serum or plasma Gold, Red or Green-top Same as All Tests above Same as all tests Daily, 8 LDL Direct measurement 0.5 ml serum or plasma Gold, Red, Lavender (EDTA) or Green Send to lab ASAP Same as all tests Form Page 13 of 19

14 Lamotrigine (Lamictal) Serum or plasma Red, Lavender (EDTA) or Green Same as All Tests above USE OF SST TUBE Latex-Specific IgE Serum Gold or Red-top Same as All tests above Same as all tests ; gross lipemia Lead, Blood (Adult) 7 ml Whole blood Royal Blue (EDTA) Same as All Tests above Clotted specimen Lipids Serum or plasma Gold or Red-top, Green Same as All Tests above Pt. must be fasting Pt. not fasting Daily, 8 Lead, Blood (Pediatric) 3 ml Whole Blood (preferred) or 0.5 ml capillary 5 ml Urine, Random or 24 hour aliquot Royal Blue (EDTA) Microtainer, Tan Same as All Tests above Clotted specimen Lead, Urine Urine cup or 24 hr. Same as All Tests above Same as All Tests above Legionella pneumonia AB Serum Gold or Red-top Same as All Tests above Same as all tests ; gross lipemia Lipase Serum or plasma Gold, Red-top, Green Same as All Tests above Same as All Tests above Daily, 8 Lithium Serum Red-top tube Same as All Tests above Same as All Tests above Daily, 8 Lupus Anticoagulant Comprehensive Panel This is not the test for LE or SLE; see ANA or SLE 6 ml frozen plasma (minimum 3 ml) Blue-top tubes Includes aptt-la; dilute drvvt; dpt; thrombin time. Reflex to extended studies if indicated. Avoid warfarin therapy for 2 weeks prior and heparin therapy for 2 days prior to testing. Hemolysis; clotted specimen;. Luteinizing Hormone (LH) Serum or Plasma Red, Green or Gel Same as All Tests above Same as All Tests above Daily, 8 Lyme Disease Ab, Total 2 ml Serum Gold or Red-top Tests for either IgG or IgM. Does not differentiate. Hemolysis; gross lipemia Lyme Disease Ab, IgM 2 ml Serum Gold or Red-top Same as All Tests above Hemolysis; gross lipemia Lyme Disease Ab with Reflex to Western Blot on Positives 2 ml Serum Gold or Red-top Same as All Tests above Hemolysis; gross lipemia Lyme Disease PCR 5 ml Whole Blood, 1 ml CSF or Synovial Fluid Yellow top (ACD) tube or Sterile container Same as All Tests above Clotted blood; Heparin tube Lyme Disease PCR 1 ml Urine, or skin biopsy Sterile container Same as All Tests above Same as All Tests above Lyme Disease Western Blot 1 ml Serum Gold or Red-top Same as All Tests above Hemolysis; Lipemia Lyme Disease Western Blot 2 ml CSF or Synovial Fluid Green-top tube (heparin) for Synovial fld; Sterile CSF Same as All Tests above Hemolysis; Lipemia tube Magnesium, serum Serum or plasma Gold or Red or Green-top Same as All Tests above Same as All Tests above Daily, 8 or Form Page 14 of 19

15 Measles (Rubeola) IgM/IgG Serum 0.5 ml Gold or Red-top Same as All Tests above Same as All Tests above; gross lipemia Mercury, Blood 7 ml Whole Blood Royal blue top tube (EDTA) Same as All Tests above Clotted specimen Mercury, Urine 5 ml Urine, Random or Urine cup or 24 hr. Same as All Tests above Same as All Tests above 24 hour aliquot Methylmalonic acid (MMA) 2 ml Serum or plasma Gold or Red or Green-top Send to lab ASAP Same as All Tests above Microalbumin, Random urine Urine, 10 ml Urine cup Same as All Tests above Bloody specimen Microalbumin 24 hour urine 24 hr. urine, 10 ml aliquot 24 hr. urine container, no preservative. ph must be 4-7 Same as All Tests above Bloody specimen Mononucleosis Screen Serum, whole blood Gold or Red-top, Lavender Same as All Tests above Same as All Tests above (Monospot) (EDTA) Mumps IgM or IgG Serum 0.5 ml Gold or Red-top Same as All Tests above Same as All Tests ; lipemia Norpace (Disopyramide) Serum or plasma, 1 ml Red, Lavender (EDTA) or Green ; No gel tubes Same as All Tests above Gel tube, hemolysis, lipemia, icterus Oligoclonal Bands Serum RT AND frozen CSF 0.5 ml for each Gold, Red-top tube and CSF Same as All Tests above Serum and CSF not collected within 8 of each other; Specimens not frozen Opiates Urine 4 oz. Urine container Same as All Tests above Same as all tests Osmolality, serum Serum 2 ml Gold or Red-top, Green Same as All Tests above Same as All Tests above Osmolality, urine Urine 4 oz. Urine container Same as All Tests above Same as All Tests above Parathyroid Hormone, Intact PTH Partial Thromboplastin Time (PTT) Plasma, 1.5 ml Lavender-top Same as All Tests above Whole blood; hemolysis; specimen not refrigerated Plasma Blue-top tube Draw waste tube before Same as All tests above Daily, 8 specimen tube if using butterfly. PCR (DNA Based) Whole blood; top 200uL; minimum 5 ml Purple, green or yellow, kit available Same as All Tests above Same as All tests above Reference Lab, 10 days Phenobarbital Serum or plasma; No gel tubes Gold, Red-top or Green-top Same as All Tests above Gel tube Phosphorus, serum Serum or plasma Gold or Red or Green-top Same as All Tests above Same as All Tests above Daily, 8 Form Page 15 of 19

16 Platelet Function Analysis PFA Blue-top tube, Priority Label; collect 3 tubes Send to Lab immediately No caffeine or fatty foods 4 prior to collection; many other drugs affect the test. Let lab know what drugs patient takes. Samples cannot be accepted from difficult venipuncture. Cannot test on patients taking Plavix (clopidogrel); can only be collected in SL or LP to minimize transit time and platelet disruption. Potassium (K), serum Serum or plasma Gold or Red or Green-top Same as All Tests above Same as All Tests above Daily, 8 or Potassium (K), urine Random or 24 Random urine or 24 hr. aliquot Urine cup or 24 hr. Same as All Tests above Same as All Tests above Daily, 8 Prealbumin Serum or plasma Gold or Red or Green-top Same as All Tests above Same as All Tests above Daily, 8 Progesterone 1 ml Serum Gold or Red-top Same as All Tests above Same as All Tests above Prolactin Serum or plasma Gold, Red-top tube Same as All Tests above Fasting specimen preferred Same as All Tests above Daily, 8 Proic Acid Phosphatase PSA Protein C Activity (Functional) Serum Gold or Red-top Same as All Tests above Same as All Tests above 2 ml frozen plasma Blue-top tube Do not draw from arm with Avoid warfarin therapy for hemolysis; thawed specimen; 2 weeks and heparin therapy clotted specimen for 2 days prior to collection. Should not be used for patients receiving thrombin inhibitors such as hirudin and argatroban. Protein C Antigen (Immunologic) 2 ml frozen plasma Blue-top tube Do not draw from arm with Avoid warfarin therapy for 2 weeks prior to collection. Should not be used for patients receiving thrombin inhibitors such as hirudin and argatroban. hemolysis; thawed specimen; clotted specimen Protein C Panel (Antigen and Activity) 3 ml frozen plasma Blue-top tube Do not draw from arm with Avoid warfarin therapy for 2 weeks and heparin therapy for 2 days prior to collection. Should not be used for patients receiving thrombin inhibitors such as hirudin and argatroban. hemolysis; thawed specimen; clotted specimen Form Page 16 of 19

17 Protein S Antigen (Free and Total) 2 ml frozen plasma Blue-top tube Do not draw from arm with Protein S Functional 2 ml frozen plasma Blue-top tube Do not draw from arm with Protein S Panel (Antigen and Functional) Protein Electrophoresis, Serum (PE, SPE, SPEP) Protein Electrophoresis, Urine (UPE, UPEP) Prothrombin Time (Protime; PT) 3 ml frozen plasma Blue-top tube Do not draw from arm with Avoid warfarin therapy for 2 weeks and heparin therapy for 2 days prior to collection. Avoid warfarin therapy for 2 week prior to collection. Avoid warfarin therapy for 2 weeks and heparin therapy for 2 days prior to collection. hemolysis; thawed specimen; clotted specimen hemolysis; thawed specimen; clotted specimen hemolysis; thawed specimen; clotted specimen Serum, 2 ml Gold or Red-top Same as All tests above Same as All Tests above 20 ml Urine, Random or 24 hr. aliquot Urine cup or 24 hr. Same as All tests above Same as All tests above Plasma Blue-top tube Draw waste tube before Same as All tests above Daily, 8 LP - Whole blood LP - Fingerstick specimen tube if using butterfly. Reticulocyte count Whole blood Lavender-top Same as All Tests above Same as All Tests above Daily, 8 Rheumatoid factor (RA Serum Gold or Red-top Same as All Tests above Same as All Tests above Daily, 8 Latex) Ro (anti-ro; SSA) see Sjogren s antibodies RPR 1 ml serum or plasma Gold or Red or Green-top or Lavender-top Plasma specimens must be at least half full tubes to avoid anticoagulant interference or dilution. Same as All Tests above; lipemia RSV Nasopharyngeal washes or aspirates Sterile, leak proof container Nasopharyngeal wash or aspirate using routine collection. Nasal swabs are not considered as sensitive for RSV detection. Same as All Tests above Daily, 8 Rubella antibody IgG/IgM Serum, 0.5 ml Gold or Red-top Same as All tests above Same as All Tests above; lipemia Salicylates Serum or Plasma Red, Green or Gel Same as All tests above Same as All tests above Daily, 8 or Serum Protein Electrophoresis Serum, 2 ml Gold or Red-top Same as All tests above Same as All Tests above Sjogren s antibodies (SSA, SSB) (also known as Ro and La) Serum, 1 ml Gold or Red-top Same as All tests above Hemolysis, Lipemia, Icterus SLE panel A 4 ml serum Gold or Red-top Includes: anti-rnp, anti-sm, anti-dna, antichromatin, Sjogren s antibodies, RA Latex SLE panel B 4 ml serum Gold or Red-top Includes: ANA, anti-dna, antichromatin, C3, C4 Same as All tests above Same as All tests above Form Page 17 of 19

18 Sodium (Na), serum Serum or plasma Gold or Red or Green-top Same as All tests above Same as All Tests above Daily, 8 or Sodium, urine Random urine or 24 hr. Urine cup or 24 hr. Same as All Tests above Same as All Tests above Daily, 8 Random or 24 hour aliquot T3 Triiodothyronine 0.5 ml serum or plasma Red-top or Green-top Same as all tests above Same as All Tests above T4 Thyroxine 0.5 ml serum or plasma Gold, Red-top or Green-top Same as All tests above Same as All Tests above Daily, 8 Testosterone, Total Serum, 0.8 ml Gold or Red-top Same as All tests above Same as All Tests above Testosterone, Free and Total Serum, 2 ml Gold or Red-top Same as All tests above Gross lipemia, recently administered isotopes Testosterone, Free and Weakly Bound Serum, 1.2 ml Gold or Red-top Same as All tests above Gross lipemia, recently administered isotopes Tetanus Ab Serum, 2 ml Gold or Red-top Same as All tests above Same as All Tests above; lipemia Thyroglobulin (level) 3 ml serum or heparinized plasma Gold or Red or Green-top Test includes Thyroglobulin quantitation and Same as All tests above Thyroglobulin antibodies Thyroglobulin antibodies 1 ml serum Gold or Red-top Same as All Tests above Hemolysis Thyroid Profile Serum or plasma, 2 ml Gold or Red or Green-top Same as All Tests above Same as All Tests above Daily, 8 Thyroid Stimulating Hormone 1 ml serum or plasma Gold or Red or Green-top Same as All Tests above Same as All Tests above Daily, 8 (TSH) TIBC - see Iron Binding Capacity Total Protein, Serum Serum or Plasma Gold or Red or Green-top Same as All Tests above Same as All Tests above Daily, 8 Toxoplasma antibody IgG Serum, 1 ml Gold or Red-top Same as All tests above Hemolysis; lipemia Toxoplasma antibody IgM Serum, 1 ml Gold or Red-top Same as All tests above Hemolysis; lipemia Transferrin Saturation - See Iron Binding Capacity Triglycerides Serum or plasma Gold or Red or Green-top Same as All tests above Fasting specimen required Same as All Tests above Daily, 8 Troponin I Plasma, tube filled to Green-top, Same as All tests above Same as All Tests above Daily, 8 or capacity, serum Gold or Red Uric Acid, Fluid Body fluid Body fluid in green top tube Same as All tests above Same as All tests above Daily, 8 Uric Acid, serum Serum or plasma Gold or Red or Green-top Same as All tests above Same as All tests above Daily, 8 Uric Acid, Random Urine Urine, random Urine cup Same as All tests above Same as All tests above Daily, 8 Form Page 18 of 19

19 Uric Acid, 24 hour Urine Urine, 24 hour aliquot 24 hr. container, no Same as All tests above Same as All tests above Daily, 8 preservative Varicella Zoster (VZ; Herpes zoster) IgG Serum, 1 ml Gold or Red-top Same as All tests above Hemolysis; lipemia Vitamin D (25 hydroxy; Vitamin D3) Plasma, 0.5 ml Gold or Red or Green-top or Lavender-top Same as All tests above Same as All Tests above Vancomycin Serum or plasma, 0.5 ml Gold or Red or Green-top Trough within 15 mins prior to dose. No peak required. Same as All Tests above Daily, 8 or Wellbutrin (Bupropion) level Serum, 3 ml Red-top tube, or Green-top Same as All tests above Same as All tests above Reason for Revision: 07/11/18 JE deleted Acetone and Cardiac Markers, added BetaHydroxybutyrate, updated container type for several analytes, Phenobarbital sent to Reference Lab, minor format edits Form Page 19 of 19