Chester County Hospital - Depratment of Pathology and Laboratory Medicine Laboratory Sample Requirements and Turnaround Time

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1 Chester County Hospital - Depratment of Pathology and Laboratory Medicine Laboratory Sample Requirements and Turnaround Time TEST NAME ACETAMINOPHEN (TYLENOL) 7 ML RED TOP MINIMUM ACETONE Adenovirus Reference Lab Nasoswab ALBUMIN, SERUM ALCOHOL SERUM ETHANOL (ETOH) RED 7 ML DO NOT REMOVE CAP, MINIMUM ALDOLASE ALKALINE PHOSPHATASE ALPHA - FETOPROTEIN QUAD SCREEN SPECIAL FORM MUST BE COMPLETED BY PATIENT RESULTED 2-3 DAYS AFTER ALPHA FETOPROTEIN TUMOR MARKER ALT (SGPT) AMMONIA VENOUS SPECIMEN RECOMMENDED 7 ML GREEN TUBE/ICE MINIMUM AMYLASE, SERUM ANA (ANTI NUCLEAR ANTIBODY) 2 ML SERUM RED TOP 7 ML RED ROUTINE - 1 RUN PER DAY UNLESS REQUESTED ANGIOTENSIN CONVERTING ENZYME (ACE) ANTI-CENTROMERE ANTIBODY 2 ML SERUM RED TOP 7 ML RED ROUTINE - 1 RUN PER DAY UNLESS REQUESTED

2 ANTI-NEUTROPHIL CYTO AB, IGG (ANCA) ANTITHROMBIN, ENZYMATIC (ACTIVITY) 5 ML BLUE aptt (ACTIVATED PARTIAL THROMBOPLASTIN TIME) ASPIRIN RESISTANCE TEST SPECIAL TUBE, CONTACT LAB AT AST (SGOT) Babesia macrotti Reference Lab ACD tube BASIC METABOLIC PANEL BENCE-JONES PROTEIN, QUANT, URINE 24 HR URINE W NO PRESERVATIVE- REFRIGERATE DURING RESULTED 1-5 DAYS AFTER BETA-2-MICROGLOBULIN (SERUM) BILIRUBIN, DIRECT 7 ML SST PROTECT FROM LIGHT MINIMUM BILIRUBIN, TOTAL AGE 22 DAYS & OVER 7 ML SST MINIMUM REQUIREMENT = 2 ML BLOOD BLOOD PARASITE SCREEN WHOLE BLOOD EDTA SIZE LAV TUBE ANY ROUTINE - 4 Hours BNP 5 ML LAVENDER BODY FLUID CELL COUNT ANTI-COAGULATED SPECIMEN EDTA OR HEPARIN Bordetella pertussis Reference Lab Nasoswab BUN

3 C1-ESTERASE INHIBITOR RESULTED 1-4 DAYS AFTER C3, SERUM MINIMUM C4, SERUM MINIMUM CA 125 CA 15-3 CA 19-9 CA CALCITONIN CALCIUM TOTAL, SERUM CALCIUM, IONIZED CALCULI (STONE) ANALYSIS SUBMIT STONE IN A CLEAN DRY CONTAINER. STATE ANATOMIC SOURCE OF Test sent Mon-Fri at 2 PM - Resulted 1-4 days CARBAMAZEPINE (TEGRETOL) TROUGH - 1/2 HOUR BEFORE NEXT DOSE 7 ML RED TOP TUBE MINIMUM CARBON DIOXIDE (CO2) CARBOXY-HEMOGLOBIN HEPARINIZED GREEN TOP TUBE CARDIOLIPIN ANTIBODIES CATECHOLAMINES PLASMA GREEN ON ICE. BLOOD MUST BE COLLECTED RESULTED 1-4 DAYS AFTER AFTER PATIENT HAS BEEN IN

4 CATECHOLAMINES, 24 HR URINE 24 HR URINE W NO PRESERVATIVE- REFRIGERATE DURING CBC COMPLETE BLOOD COUNT INCLUDES: BLOOD CELL PROFILE AND WBC WHOLE BLOOD EDTA LAV TOP PED: LAV MICROTAINER MINIMUM REQUIREMENTS: 5 CEA MINIMUM REQUIREMENT = 5 ML BLOOD CELIAC DISEASE REFLEX CASCADE (antibodies performed per algorithm) RESULTED 2-6 DAYS AFTER Chlamydia pneumoniae Reference Lab ACD tube/nasoswab Chlamydia trachomatis (genital) Molecular Microbiology Probetec vial/ Liquid cytology/ Urine (males only) Mon/Wed/Friday - 1 X per day Chlamydia trachomatis non genital Reference Lab ACD tube/urine/swab in UTM CHLORIDE, SERUM MINIMUM CHOLESTEROL, SERUM TOTAL SUGGEST FASTING 7 ML SST TUBE MINIMUM CHROMOSOME ANALYSIS (GENETIC DISORDER) CONTACT LABORATORY RESULTED 3-10 DAYS AFTER CHROMOSOME ANALYSIS (PRODUCTS OF CONCEPTION) CONTACT LABORATORY TEST SENT MON-FRI AT 2 PM, RESULT TIME VARIES WITH TYPE OF SAMPLE CK, TOTAL MINIMUM CK-MB/CK RELATIVE INDEX PART OF CK-MB GROUP SEE CK SPECIMEN REQ. MINIMUM REQUIREMENT = 4 ML BLOOD COLD AGGLUTININS TITER 7 ML WARMED SST TUBE Test sent Mon-Fri at 2 PM - Resulted 2- KEEP WARM PRIOR TO 5 days after sending TRANSPORT COMPLEMENT ACTIVITY TOTAL (EIA) COMPREHENSIVE METABOLIC PANEL

5 CORONARY RISK PROFILE (LIPID PROFILE) 14 HR FAST RECOMMENDED MINIMUM REQUIREMENT = 5 ML CORTISOL, 24 HR URINE 24 HR URINE W NO PRESERVATIVE- RESULTED 1-4 DAYS AFTER REFRIGERATE DURING CORTISOL, SERUM C-PEPTIDE C-REACTIVE PROTEIN, QUANTITATE CREATININE CLEARANCE, 24 HR 24 HOUR URINE- NO PRESERVATIVES-REFRIGERA TE THROUGH COLLECTION. CREATININE, SERUM REQUIREMENT = 2 ML CREATININE, URINE RANDOM 2 ML RANDOM URINE CRP HIGH SENSITIVITY CRYSTALS BODY FLUID ANTI-COAGULATED SPECIMEN, EDTA OR HEPARIN CYCLIC CITRULLINATED PEPTIDE AB, IGG (CCP) CYSTIC FIBROSIS GENOTYPE (32 mutations) 5 ML LAV TUBE RESULTED 3-7 DAYS AFTER Cytomegalovirus Reference Lab ACD tube/urine D-DIMER DHEA SULFATE DIGOXIN SPECIAL PREP DRAW BEFORE NEXT DOSE RED TUBE (7ML) MINIMUM

6 DNA ANTIBODIES, DOUBLE STRANDED RESULTED 1-3 DAYS AFTER DRUG SCREEN, URINE ONLY PERFORMED ON INPATIENTS AND ED RANDOM URINE 5 ML MINIMUM REQUIREMENT = 2 ML URINE STAT FOR ED ONLY ELECTROLYTES, RANDOM URINE, URINE CHLORIDE, SODIUM, POTASSIUM, SPECIAL PREP 5-10 ML RANDOM URINE MINIMUM REQUIREMENT = 2 ML ELECTROLYTES, SERUM Epstein Barr Reference Lab ACD tube/urine EPSTEIN-BARR VIRUS ABS (includes antibodies to capsid antigens, nuclear antigens, and ERYTHROCYTE SEDIMENTATION RATE (ESR) (SED RATE) WHOLE BLOOD EDTA LAV TOP PED: LAV MICROTAINER 500 UL MINIMUM ROUTINE - 4 Hours ESTRADIOL (MEN AND CHILDREN) RESULTED 1-4 DAYS AFTER ESTRADIOL FREE BY ed/lc-ms/ms RESULTED 1-6 DAYS AFTER EXPOSURE HEPATITIS PROFILE Reference Lab 2-S EXPOSURE HEPATITIS PROFILE 2-S APPROVED BY ID EXPOSURE RPR BLOOD BANK APPROVED BY ID F-ACTIN SMOOTH MUSCLE ABS, IGG WITH REFLEX RESULTED 1-5 DAYS AFTER FACTOR ASSAYS FVIII, FIX FACTOR V LEIDEN LAV TUBE FERRITIN

7 FETAL FIBRONECTIN (FFN) CALL LAB FIBRIN DEGRADATION PRODUCTS, FPS FIBRINOGEN FOLATE (FOLIC ACID) FASTING SPECIMEN PERFORMED FREE T-3 FREE T-4 FSH GENTAMICIN, PEAK SPECIAL PREP 15 MIN. AFTER A MIN. DRUG INFUSION RED (7 ML) GENTAMICIN, TROUGH SPECIAL PREP MIN. BEFORE IV DOSE RED TOP TUBE MINIMUM GGT GLUCOSE - 6- PHOS. DEHYDROGENASE 5 ML LAV GLUCOSE TOLERANCE TESTING (ORAL), GESTATIONAL AND CALL LAB AT BY APPOINTMENT ONLY. PERFORMED ON OUTPATIENTS ONLY. GLUCOSE, 1 HR POST GLUCOLA DOSE (FOR GESTATIONAL PATIENTS 1 HR AFTER GLUCOLA BY APPOINTMENT ONLY GLUCOSE, 2 HR. PP DRAW 2 HRS. AFTER DESIGNATED MEAL 7 ML SST TUBE MINIMUM GLUCOSE, CSF 2 ML CSF MINIMUM REQUIREMENT = 1 ML CSF GLUCOSE, FLUID 2 ML FLUID IN RED TOP MINIMUM REQUIREMENT = 1 ML FLUID

8 GLUCOSE, RANDOM/ FASTING HAPTOGLOBIN ED MINIMUM HCG, TUMOR MARKER HCG, QUALITATIVE SERUM MINIMUM REQUIREMENT = 4 ML BLOOD HCG, QUALITATIVE URINE BLOOD BANK FIRST MORNING URINE SPECIMEN REFRIGERATE HCG, QUANTITATIVE MINIMUM REQUIREMENT = 4 ML BLOOD STAT/WEEKDAYS ROUTINE/TODAY HEAVY METAL SCREEN, BLOOD FULL ROYAL BLUE TOP TRACE METAL TUBE RESULTED 1-4 DAYS AFTER HEAVY METAL SCREEN, URINE 24 HR URINE W NO PRESERVATIVE- REFRIGERATE DURING RESULTED 1-4 DAYS AFTER HELICOBACTER PYLORI AB IGA HELICOBACTER PYLORI AB IGG HELICOBACTER PYLORI AB IGM RESULTED 1-3 DAYS AFTER HEMOGLOBIN A1C (GLYCOSYLATED HEMOGLOBIN) 7 ML LAVENDER TUBE MINIMUM REQUIREMENT = 2 ML BLOOD HEMOGLOBIN EVALUATION W/ REFLEX TO ELECTROPHORESIS 5 ML LAB Test sent Mon-Fri at 2 PM - Resulted 1-7 days after sending HEPATITIS B SURFACE ANTIBODY HEPATITIS C ANTIBODY Reference Lab HEPATITIS C ANTIBODY 1-2 DAYS

9 HEPATITIS PROFILE: 1-2 DAYS HEPATITIS PROFILE: Reference Lab HERPES SIMPLEX 1 & 2 (IGG AND IGM) Herpes simplex 1&2 Reference Lab CSF Herpes simplex 1&2sub-typing Reference Lab Viral swab in UTM/CSF HERPES ZOSTER (VARICELLA) IGG ANTIBODY HGE Anaplasma phagocytophilum Reference Lab ACD tube HIV SCREEN (RAPID) BLOOD BANK 5 ML LAVENDER, DAYSHIFT ONLY HIV, EXPOSURE 5 ML LAVENDER RESULTED 1-3 DAYS AFTER HME Ehrlichia chaffeensis Reference Lab ACD tube HPV subtype Reference Lab Genital specimen Human Papillomavirus Molecular Microbiology Liquid Cytology Mon/Wed/Friday - 1 X per day Human T cell Leukemia Reference Lab ACD tube IGE, QUANTITATIVE IGG SUBCLASSES RESULTED 1-3 DAYS AFTER IMMUNOFIXATION ELECTROPHORESIS GEL, SERUM RESULTED 1-5 DAYS AFTER

10 Influenza A confirmation Reference Lab Nasoswab INSULIN ANTIBODY 7 ML SST PATIENT MUST BE FASTING INSULIN, SERUM IRON PANEL IRON/TIBC L/S Ratio AMNIOTIC FLUID (CALL LAB IN ADVANCE) SENT STAT TO ADL LABS LACTIC ACID GREEN ON ICE LEAD, BLOOD TRACE METAL TUBE Legionella pneumophila Reference Lab ACD tube LIPASE LITHIUM BEFORE NEXT DOSE 7 ML RED MINIMUM LIVER PROFILE LDH, AST, ALT, ALP, GGT, TBIL, CBIL REQUIREMENT = 3 ML BLOOD LUPUS ANTICOAGULANT, MON-FRI DAYSHIFT ONLY LUTEINIZING HORMONE (LH) LYME ANTIBODY C6 PEPTIDE WITH REFLEX TO **WESTERN BLOT MICROBIOLOGY 7 ML RED TOP (NOT SST) ROUTINE - ONE RUN PER DAY MON, WED, FRI Lyme by PCR Reference Lab CSF/Joint fluid/pericardial fluid LYME DISEASE WESTERN BLOT FOR CSF CSF RESULTED 1-3 DAYS AFTER

11 MAGNESIUM, 24 HR. URINE 24 HR URINE W NO PRESERVATIVE- REFRIGERATE DURING MAGNESIUM, SERUM METANEPHRINE FRACTIONATED, URINE 24 HR URINE W NO PRESERVATIVE- REFRIGERATE DURING RESULTED 1-3 DAYS AFTER Metapneumovirus Reference Lab Nasoswab METHEMOGLOBIN HEPARINIZED ARTERIAL OR GREEN TOP ON ICE - BLOOD PROCESS IMMEDIATELY MICROALBUMIN RANDOM OR 24 HR URINE W NO PRESERVATIVE- REFRIGERATE DURING MIXING TEST Molluscum contagiosum Reference Lab ACD/Swab in UTM MONOSPOT BLOOD BANK MRSA nasal carriage Molecular Microbiology Nasal swab MUMPS VIRUS ANTIBODY (IGG) MYCOPLASMA PNEUMO. (IGG) 7 ML SST Mycoplasma pneumoniae Reference Lab ACD tube/nasoswab Neisseria gonorrhoeae Molecular Microbiology Probetec vial/ Liquid cytology/ Urine (males only) Mon/Wed/Friday - 1 X per day OSMOLARITY, SERUM OSMOLARITY, URINE RANDOM URINE

12 OXYGEN SATURATION SO2 FOR MIXED VENOUS SAMPLES IN CVU MIXED VENOUS BLOOD Parainfluenza 1-4 Reference Lab Nasoswab Parvovirus Reference Lab ACD tube PARVOVIRUS B19 ANTIBODIES RESULTED 1-3 DAYS AFTER PCO2 PART OF ARTERIAL BLOOD GAS PANEL 2 ML HEPARINIZED ARTERIAL PH, ARTERIAL BLOOD PART OF ARTERIAL BLOOD GAS PANEL 2 MLS HEPARINIZED ARTERIAL PHENOBARBITAL PHENYTOIN, (DILANTIN) SPECIAL PREP 7 ML RED TOP TUBE MINIMUM PHOSPHORUS, SERUM SUGGEST FASTING 7 ML SST MINIMUM REQUIREMENT = 2 ML BLOOD PLATELET FUNCTION ASSAY PLAVIX FUNCTION TEST CONTACT AT PO2, ARTERIAL BLOOD PART OF ABGS SPECIAL COLLECTION KIT 2 ML HEPARINIZED ARTERIAL POTASSIUM, SERUM PREALBUMIN PRIMIDONE (MYSOLINE) PROCAINAMIDE/NAPA MINIMUM

13 PROCALCITONIN GREEN PROGESTERONE PROLACTIN PROTEIN C - FUNCTIONAL ACTIVITY BLUE TOP PROTEIN ELECTROPHORESIS, SERUM PROTEIN ELECTROPHORESIS, URINE 24 HOUR COLLECTION WITHOUT PRESERVATIVE. REFRIGERATE SPECIMEN RESULTED 1-5 DAYS AFTER PROTEIN S - FUNCTIONAL ACTIVITY BLUE TOP PROTEIN, CSF COLLECTED BY PATIENT CARE PERSONNEL 2 ML CSF MINIMUM REQUIREMENT = 1 PROTEIN, TOTAL SERUM PROTEIN, TOTAL, 24 HR URINE 24 HR URINE W NO PRESERVATIVE- REFRIGERATE DURING PROTHROMBIN MUTATION 5 ML LAV PSA, FREE AND TOTAL PSA, TOTAL PT /INR PTH, INTACT 5 ML LAV TUE-FRI DAYSHIFT ONLY QUANTITATIVE IMMUNOGLOBULINS IGA-IGG-IGM

14 RAST/IMMUNOCAP 7 ML SST (MUST LIST ALLERGINS TO BE TESTED) RESULTS DEPEND ON TYPE AND NUMBER OF ALLERGENS. RENIN ACTIVITY 5 ML LAV RETICULOCYTE COUNT WHOLE BLOOD EDTA ANY SIZE LAV TOP ROUTINE - 4 Hours RHEUMATOID FACTOR BLOOD BANK ROUTINE - RUN 1 X PER DAY UNLESS REQUESTED, MON-FRI DAYSHIFT ONLY Rickettsia rickettsii RMSF Reference Lab ACD tube RPR BLOOD BANK ROUTINE - 1 RUN PER DAY UNLESS REQUESTED, MON-FRI DAYSHIFT ONLY RSV confirmation Reference Lab Nasoswab RUBELLA (IGG) RUBEOLA VIRUS ANTIBODIES (IGG) SALICYLATE (ASPIRIN) MINIMUM SCLERODERMA ANTIBODIES (SCL-70) SEROTONIN, BLOOD RESULTED 1-5 DAYS AFTER SERUM VISCOSITY 7 ML SERUM RED TOP SICKLE CELL SCREEN WHOLE BLOOD EDTA ANY SIZE LAV TOP ROUTINE - 4 Hours SJÖGREN S SYNDROME ANTIBODIES (SS-A & B) * Test sent Mon-Fri at 2 PM - RESULTED 2-3 DAYS AFTER SMEARS FOR WRIGHT STAIN FECAL LEUKOCYTES URINE FOR EOS STOOL, URINE ROUTINE - 4 Hours

15 SODIUM, SERUM T3, TOTAL TESTOSTERONE, ADULT MALE TESTOSTERONE, FREE AND TOTAL ADULT MALE THEOPHYLLINE MINIMUM REQUIREMENTS = 2 ML BLOOD THYROGLOBULIN ANTIBODIES TUE-FRI DAYSHIFT ONLY THYROGLOBULIN, QUANTITATIVE TUE-FRI DAYSHIFT ONLY THYROID PEROXIDASE (MICROSOMAL) ANTIBODY TUE-FRI DAYSHIFT ONLY TOBRAMYCIN, PEAK TOBRAMYCIN, TROUGH DRAW IMMEDIATELY PRIOR TO NEXT DOSE Toxoplasma gondii Reference Lab ACD tube TRIGLYCERIDES, SERUM HR FAST 7 ML SST MINIMUM REQUIREMENT = 2 ML BLOOD TSH tspot SERUM TEST FOR TB GREEN SENT ONLY MON - FRI. MUST BE IN LAB IN MONRING. RESULTS 2-5 DAYS. Ureaplasma Reference Lab ACD tube/urine URIC ACID, 24 HR URINE SPECIAL PREP 24 HOUR COLLECTION 6 N HCL (PRESERVATIVE)

16 URIC ACID, SERUM URINALYSIS MICROBIOLOGY URINE- COLLECT INTO A CLEAN OR STERILE CONTAINER. REFRIGERATE VALPROIC ACID (DEPAKENE) VANCOMYCIN, PEAK DRAW 2 HRS AFTER MIN DRUG INFUSION 7 ML RED MINIMUM VANCOMYCIN, RANDOM MINIMUM REQUIREMENT = 3 ML BLOOD VANCOMYCIN, TROUGH DRAW TIME MIN BEFORE NEXT DOSE 7 ML RED MINIMUM Varicella zoster Reference Lab Viral swab in UTM/CSF VARICELLA-ZOSTER AB, IGG VARICELLA-ZOSTER AB, IGG AND IGM RESULTED 1-5 DAYS AFTER VITAMIN B12 FASTING SPECIMEN PREFERRED VITAMIN D, 25 HYDROXY West Nile Virus Reference Lab ACD tube/csf Please refer to the Laboratory Service Manual for more information. Updated August 2014

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