N-Cadherin Locks Left-Right Asymmetry by Ending the Leftward Movement of Hensen s Node Cells
|
|
- Roland Gallagher
- 6 years ago
- Views:
Transcription
1 Developmental Cell, Volume 30 Supplemental Information N-Cadherin ocks eft-ight Asymmetry by Ending the eftward Movement of Hensen s Node Cells aquel V. Mendes, Gabriel G. Martins, Ana M. Cristovão, and eonor Saúde
2 Supplemental Figure S1 A Control MNCD2 AB B B C D E E F G 0h00m + 4h20m 0-4h20m 0h00m + 5h20m 0h-5h20m H I I J K M N 4h30m 7h00m 4h30m-7h00m 5h30m 8h20m 5h30m-8h20m Control MNCD2 AB Control MNCD2 AB O P U 5som V 5som shh shh Q W 3som X 3som fgf8 fgf8 cer1 cer1 S T Y 5som Z 5som snai1 snai1
3 Figure S1. oss of N-cadherin activity in the Hensen's node prolongs leftward cell movements and affects asymmetric gene expression in Hensen's node and PM. elated to Figure 2 and Figure 3. (A) A stage diagram showing photoconverted cells (red dots) on the right side of the node. Position of photoconverted cells at two time-points and their tracks in a control (B-C) and in a MNCD2 antibody-treated embryo (E-F). Overlay tracks of all cells analyzed from six controls (D) and five MNCD2 antibody-treated embryos (G) photoconverted at the beginning of stage and tracked until stage. (H) A stage diagram where photoconverted cells at stage (light-red dots) moved to the left and new cells were photoconverted (dark-red dots) on the right side of the node. Position of photoconverted cells at two timepoints and their tracks in a control (I-J) and in a MNCD2 antibody-treated embryo (-M). Overlay tracks of all cells analyzed from six controls (K) and five MNCD2 antibody-treated embryos (N) photoconverted at the beginning of stage and tracked until stage. In control embryos, 11 out of 20 labeled cells at stage crossed the midline and 0 out of 34 labeled cells at stage crossed the midline; in MNCD2 antibody-treated embryos, 7 out of 24 labeled cells at stage crossed the midline and 10 out of 16 labeled cells at stage crossed the midline. The expression of shh was asymmetric on the left side of the node in 93% (n=16) of controls (O) and in 100% (n=19) of MNCD2-treated embryos (P). The expression of fgf8 was asymmetric on the right side of the node in 80% (n=30) of controls (Q) and became bilaterally symmetric in 43% (n=37) of MNCD2-treated embryos (). In MNCD2-treated embryos the expression of
4 in the peri region was downregulated in 63% (n=30) of the embryos (T) in contrast to controls where asymmetric expression on the left peri region was found in 75% (n=12) (S). The expression of was found to be restricted to the left PM in 100% (n=5) of controls (U) and in 100% (n=5) in MNCD2-treated embryos (V). The expression of cer1 was asymmetric on the left side of the PM in 87% (n=15) of controls (W) and downregulated in 53% (n=15) of MNCD2-treated embryos (X). The expression of snai1 was asymmetric on the right side of the PM in 97% (n=31) of controls (Y) and became bilateral in 32% (n=38) of MNCD2-treated embryos (Z). Insets in all panels show a magnification of the node region. Black and white arrowheads indicate normal and abnormal expression, respectively., left;, right; AB, antibody.
5 Supplemental Figure S2 pcaggs-gfp pcaggs-n-cadyfp A B C D E F shh shh G H I J K fgf8 fgf8 M N O P Q S T 5som U V W 5som X cer1 cer1
6 Figure S2. N-cadherin overexpression on the right side of the node does not affect asymmetric gene expression. elated to Figure 3. Chicken embryos electroporated with pcaggs-gfp or pcaggs-n-cadyfp on the right side of the node at stage HH3 + and photographed at stage (A, G, M, S, D, J, P, V). The same embryos photographed at stage (B, H, N, E, K, Q) or at 5-somite stage (T, W) and in situ hybridized with shh (C, F), fgf8 (I, ) (O, ) and cer1 (U, X). Insets are magnifications of the node region. Arrowheads indicate normal gene expression. Black bracket, notochord width in a control embryo; red bracket, notochord width in a N-cadherin overexpressing embryo;, left;, right.
7 Supplemental Table S1 Table S1. Number of cells that crossed the midline at different stages of development in control vs Anti-N-cad treated embryos. elated to Figure 2. Beginning of cell tracks Nº of tracked cells Nº of cells that cross the midline during stages + Nº of cells that do NOT cross the midline # Control 27 16* Anti-N-cad 22 2* Anti-N-cad 13 n/a Control 28 n/a n/a Anti-N-cad 24 n/a n/a * The higher number of cells that cross the midline at stage in control embryos when compared to Anti-N-cad embryos is most probably due to a technical issue. In controls we managed to start the tracks at an earlier time point (spanning on average 3 hours before reaching stage + ), while in Anti-N-cad embryos we only managed to start the tracks at a later time point (spanning on average 2 hours before reaching stage + ). # The cells that did not cross the midline, either stayed on the right side of the node and were displaced anteriorly or died or divided or aggregated. n/a, not applicable.
8 Movie S1. Time-lapse movie of a chicken embryo electroporated on the right side with Kaede-NS photoconvertible fluorescent protein and treated with IgG2a control antibody. elated to Figure 2. Cells on the right side of the node were photoconverted from green to red at stage and followed for a period of 4 hours and 10 minutes after which some cells were found on the left side of the node. When the embryo reached stage, new cells on the right side of the node were photoconverted from green to red and followed over a period of 4 hours and 20 minutes, after which, no cells were found to cross to the left side of the node. Cell tracks of the photoconverted cells are shown in the end. The yellow dashed line in the first frame marks the position of the primitive pit and primitive groove, and the pink circle highlights the photoconverted region. Movie S2. Time-lapse movie of a chicken embryo electroporated on the right side with Kaede-NS photoconvertible fluorescent protein and treated with anti-n-cad antibody. elated to Figure 2. Cells on the right side of the node were photoconverted from green to red at stage and followed for a period of 2 hours after which some cells were found on the left side of the node. When the embryo reached stage, new cells on the right side of the node were photoconverted from green to red and followed over a period of 6 hours and 10 minutes, after which some cells were found to cross to the left side of the node. Cell tracks of photoconverted cells are shown in the end. The yellow dashed line in the first frame marks the position of the
9 primitive pit and primitive groove, and the pink circle highlights the photoconverted region. Movie S3. Time-lapse movie of a chicken embryo electroporated on the right side with full-length N-cadherinYFP. elated to Figure 2. The time-lapse starts at stage and finishes at stage, spanning a 7-hour period. Fluorescent cells overexpressing N-cadherin found on the right side of the node never crossed to the left side. Cell tracks of fluorescent cells are shown in the end. The yellow line in the first frame marks the position of the primitive pit and primitive groove. Movie S4. Time-lapse movie of a chicken embryo electroporated with Kaede-NS photo-convertible fluorescent protein on the right side and treated with PBS. elated to Figure S1 and Figure 2. Cells on the right side of the node were photo-converted from green to red at stage and followed for a period of 4 hours and 20 minutes after which some cells were found on the left side of the node. When the embryo reached stage, new cells on the right side of the node were photo-converted from green to red and followed over a period of 2 hours and 30 minutes, after which no cells were found to cross to the left side of the node. Cell tracks of the photoconverted cells at stage and later at stage are shown in the end. The yellow dashed line in the first frame marks the position of the primitive streak, and the pink circle highlights the region of photoconverted cells.
10 Movie S5. Time-lapse movie of a chicken embryo electroporated with Kaede-NS photo-convertible fluorescent protein on the right side and treated with the N-cadherin blocking MNCD2 antibody. elated to Figure S1 and Figure 2. Cells on the right side of the node were photo-converted from green to red at stage and followed for a period of 5 hours and 20 minutes after which some cells were found on the left side of the node. When the embryo reached stage, new cells on the right side of the node were photo-converted from green to red and followed over a period of 2 hours and 50 minutes, after which some cells were found to cross to the left side of the node. Cell tracks of the photoconverted cells at stage and later at stage are shown in the end. The yellow dashed line in the first frame marks the position of the primitive streak, and the pink circle highlights the region of photoconverted cells.
SUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb3443 In the format provided by the authors and unedited. Supplementary Figure 1 TC and SC behaviour during ISV sprouting. (a) Predicted outcome of TC division and competitive Dll4-Notch-mediated
More informationCD4 and CD8 T cells show a similar accumulation in the tumor stroma.
Fig S1 CD4 Fibronectin EpCM CD8 CD4 and CD8 T cells show a similar accumulation in the tumor stroma. Fluorescently-labeled CD4 (CMFD, green) and CD8 (Hoechst, yellow) T cells were added to a human lung
More informationSupplementary Figure 1. A microarray screen of organizers compared to non-organizer tissue reveals a putative organizer gene set.
Supplementary Figure 1. A microarray screen of organizers compared to non-organizer tissue reveals a putative organizer gene set. (a, b) Venn diagrams of 31 enriched (a) and 17 depleted (b) genes significantly
More informationAhtiainen et al., http :// /cgi /content /full /jcb /DC1
Supplemental material JCB Ahtiainen et al., http ://www.jcb.org /cgi /content /full /jcb.201512074 /DC1 THE JOURNAL OF CELL BIOLOGY Figure S1. Distinct distribution of different cell cycle phases in the
More informationSupplementary Fig. 1 Blocking shh function at the protein level confirms its role as a guidance cue for postcommissural axons.
Supplementary Fig. 1 Blocking shh function at the protein level confirms its role as a guidance cue for postcommissural axons. As an alternative method to demonstrate the role of shh as a guidance cue
More informationT H E J O U R N A L O F C E L L B I O L O G Y
Supplemental material Brooks and Wallingford, http://www.jcb.org/cgi/content/full/jcb.201204072/dc1 T H E J O U R N A L O F C E L L B I O L O G Y Figure S1. Quantification of ciliary compartments in control
More informationArterial and Venous Progenitors of the Major Axial Vessels Originate at Distinct Locations
Article Arterial and Venous Progenitors of the Major Axial Vessels Originate at Distinct Locations Vikram Kohli, 1,4 Jennifer A. Schumacher, 1,4 Sharina Palencia Desai, 1,3 Kira Rehn, 1 and Saulius Sumanas
More informationSUPPLEMENTARY INFORMATION
Supplementary Information included with Nature MS 2008-02-01484B by Colantonio et al., entitled The dynein regulatory complex is required for ciliary motility and otolith biogenesis in the inner ear. This
More informationSupplementary Figure 1. Electroporation of a stable form of β-catenin causes masses protruding into the IV ventricle. HH12 chicken embryos were
Supplementary Figure 1. Electroporation of a stable form of β-catenin causes masses protruding into the IV ventricle. HH12 chicken embryos were electroporated with β- Catenin S33Y in PiggyBac expression
More informationSUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb2988 Supplementary Figure 1 Kif7 L130P encodes a stable protein that does not localize to cilia tips. (a) Immunoblot with KIF7 antibody in cell lysates of wild-type, Kif7 L130P and Kif7
More informationWnt signaling and PKA control Nodal expression and left-right determination in the chick embryo
Development 128, 3189-3195 (2001) Printed in Great Britain The Company of Biologists Limited 2001 DEV9773 3189 Wnt signaling and PKA control Nodal expression and left-right determination in the chick embryo
More informationWhen you see this diagram, remember that you are looking at the embryo from above, through the amniotic cavity, where the epiblast appears as an oval
When you see this diagram, remember that you are looking at the embryo from above, through the amniotic cavity, where the epiblast appears as an oval disc 2 Why the embryo needs the vascular system? When
More informationa 0,8 Figure S1 8 h 12 h y = 0,036x + 0,2115 y = 0,0366x + 0,206 Labeling index Labeling index ctrl shrna Time (h) Time (h) ctrl shrna S G2 M G1
(GFP+ BrdU+)/GFP+ Labeling index Labeling index Figure S a, b, y =,x +, y =,x +,,,,,,,, Time (h) - - Time (h) c d S G M G h M G S G M G S G h Time of BrdU injection after electroporation (h) M G S G M
More informationSupplemental Information. Myocardial Polyploidization Creates a Barrier. to Heart Regeneration in Zebrafish
Developmental Cell, Volume 44 Supplemental Information Myocardial Polyploidization Creates a Barrier to Heart Regeneration in Zebrafish Juan Manuel González-Rosa, Michka Sharpe, Dorothy Field, Mark H.
More informationJohn Nguyen, Nozomi Nishimura, Robert Fetcho, Costantino Iadecola, Chris B. Schaffer
Supplemental figures and text for Occlusion of cortical ascending venules causes blood flow decreases, reversals in flow direction, and vessel dilation in upstream capillaries John Nguyen, Nozomi Nishimura,
More informationSUPPLEMENTARY INFORMATION
Supplementary Figure 1. Formation of the AA5x. a, Camera lucida drawing of embryo at 48 hours post fertilization (hpf, modified from Kimmel et al. Dev Dyn. 1995 203:253-310). b, Confocal microangiogram
More informationÁngel Raya* and Juan Carlos Izpisúa Belmonte
Left right asymmetry in the vertebrate embryo: from early information to higher-level integration Ángel Raya* and Juan Carlos Izpisúa Belmonte Abstract Although vertebrates seem to be essentially bilaterally
More informationSUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb3200 Supplementary Figure 1 Expression analysis of stomach markers in gutlike structure. (a) Differentiation scheme of gut-like structure formation from embryonic stem cells. (b) RT-PCR
More informationBiology Open (2014) 000, 1 10 doi: /bio
(2014) 000, 1 10 doi:10.1242/bio.201410041 Supplementary Material Michael Brauchle et al. doi: 10.1242/bio.201410041 Fig. S1. Alignment of GFP, sfgfp, egfp, eyfp, mcherry and mruby2. Sequence-based alignment
More informationSupplemental Information. Otic Mesenchyme Cells Regulate. Spiral Ganglion Axon Fasciculation. through a Pou3f4/EphA4 Signaling Pathway
Neuron, Volume 73 Supplemental Information Otic Mesenchyme Cells Regulate Spiral Ganglion Axon Fasciculation through a Pou3f4/EphA4 Signaling Pathway Thomas M. Coate, Steven Raft, Xiumei Zhao, Aimee K.
More informationSUPPLEMENTARY INFORMATION
DOI:.38/ncb3399 a b c d FSP DAPI 5mm mm 5mm 5mm e Correspond to melanoma in-situ Figure a DCT FSP- f MITF mm mm MlanaA melanoma in-situ DCT 5mm FSP- mm mm mm mm mm g melanoma in-situ MITF MlanaA mm mm
More informationSupplementary Figures
Supplementary Figures Supplementary Figure 1. nrg1 bns101/bns101 embryos develop a functional heart and survive to adulthood (a-b) Cartoon of Talen-induced nrg1 mutation with a 14-base-pair deletion in
More informationTanimoto et al., http ://www.jcb.org /cgi /content /full /jcb /DC1
Supplemental material JCB Tanimoto et al., http ://www.jcb.org /cgi /content /full /jcb.201510064 /DC1 THE JOURNAL OF CELL BIOLOGY Figure S1. Method for aster 3D tracking, extended characterization of
More informationTbx6 Regulates Left/Right Patterning in Mouse Embryos through Effects on Nodal Cilia and Perinodal Signaling
Tbx6 Regulates Left/Right Patterning in Mouse Embryos through Effects on Nodal Cilia and Perinodal Signaling Anna-Katerina Hadjantonakis 2, Elinor Pisano 1, Virginia E. Papaioannou 1 * 1 Department of
More informationSUPPLEMENTARY INFORMATION
SUPPLEMENTARY INFORMATION Supplementary Figure 1. The expression of ephrin-b2 H2BGFP persists in the post-hearingonset organ of Corti and is specifically restricted to supporting cells. Sox2 immunolabeling
More informationJ. Cell Sci. 129: doi: /jcs : Supplementary information
Movie 1. AgLDL is contained in small sub-regions of the lysosomal synapse that are acidic. J774 cells were incubated with agldl dual labeled with a ph sensitive and a ph insensitive fluorophore for 1 hr.
More informationSupplemental Figure 1. Quantification of proliferation in thyroid of WT, Ctns -/- and grafted
Supplemental Figure 1. Quantification of proliferation in thyroid of WT, Ctns -/- and grafted Ctns -/- mice. Cells immunolabeled for the proliferation marker (Ki-67) were counted in sections (n=3 WT, n=4
More informationSupplementary Figure 1. Baf60c and baf180 are induced during cardiac regeneration in zebrafish. RNA in situ hybridization was performed on paraffin
Supplementary Figure 1. Baf60c and baf180 are induced during cardiac regeneration in zebrafish. RNA in situ hybridization was performed on paraffin sections from sham-operated adult hearts (a and i) and
More informationShh signaling guides spatial pathfinding of raphespinal tract axons by multidirectional repulsion
ORIGINAL ARTICLE Cell Research (2012) 22:697-716. 2012 IBCB, SIBS, CAS All rights reserved 1001-0602/12 $ 32.00 www.nature.com/cr npg Shh signaling guides spatial pathfinding of raphespinal tract axons
More informationDevelopmental Biology
Developmental Biology 362 (2012) 141 153 Contents lists available at SciVerse ScienceDirect Developmental Biology journal homepage: www.elsevier.com/developmentalbiology Dual function of suppressor of
More informationSupplementary Figure S1: TIPF reporter validation in the wing disc.
Supplementary Figure S1: TIPF reporter validation in the wing disc. a,b, Test of put RNAi. a, In wildtype discs the Dpp target gene Sal (red) is expressed in a broad stripe in the centre of the ventral
More informationSupplementary Figure 1: Signaling centers contain few proliferating cells, express p21, and
Supplementary Figure 1: Signaling centers contain few proliferating cells, express p21, and exclude YAP from the nucleus. (a) Schematic diagram of an E10.5 mouse embryo. (b,c) Sections at B and C in (a)
More informationSUPPLEMENTARY MATERIAL. Bimodal distribution of motility and cell fate in Dictyostelium discoideum
doi: 10.1387/ijdb.113384ps www.intjdevbiol.com SUPPLEMENTRY MTERIL corresponding to: imodal distribution of motility and cell fate in ictyostelium discoideum PVN GOURY-SISTL, VIYNN NNJUNIH and GOPL PNE
More informationSupplementary material Legends to Supplementary Figures Figure S1. Figure S2. Figure S3.
Supplementary material Legends to Supplementary Figures. Figure S1. Expression of BICD-N-MTS fusion does not affect the distribution of the Golgi and endosomes. HeLa cells were transfected with GFP-BICD-N-MTS
More informationCorrespondence: mirna regulation of Sdf1 chemokine signaling provides genetic robustness to germ cell migration
Correspondence: mirna regulation of Sdf1 chemokine signaling provides genetic robustness to germ cell migration Alison A. Staton, Holger Knaut, and Antonio J. Giraldez Supplementary Note Materials and
More informationFig. S1. Subcellular localization of overexpressed LPP3wt-GFP in COS-7 and HeLa cells. Cos7 (top) and HeLa (bottom) cells expressing for 24 h human
Fig. S1. Subcellular localization of overexpressed LPP3wt-GFP in COS-7 and HeLa cells. Cos7 (top) and HeLa (bottom) cells expressing for 24 h human LPP3wt-GFP, fixed and stained for GM130 (A) or Golgi97
More informationactivation with anti-cd3/cd28 beads and 3d following transduction. Supplemental Figure 2 shows
Supplemental Data Supplemental Figure 1 compares CXCR4 expression in untreated CD8 + T cells, following activation with anti-cd3/cd28 beads and 3d following transduction. Supplemental Figure 2 shows the
More informationAxis Formation and Mesoderm Induction
Developmental Biology Biology 4361 Axis Formation and Mesoderm Induction October 27, 2005 Amphibian anteroposterior specification polarized eggs animal/vegetal pigment yolk v. clear cytoplasm mitochondrial
More informationT H E J O U R N A L O F C E L L B I O L O G Y
Supplemental material Chen et al., http://www.jcb.org/cgi/content/full/jcb.201210119/dc1 T H E J O U R N A L O F C E L L B I O L O G Y Figure S1. Lack of fast reversibility of UVR8 dissociation. (A) HEK293T
More informationThe M Ruler (Figure 6) Figure 6 M Ruler (Figure 7)
The M Ruler Dr. Alain Méthot, D.M.D. M.Sc. It has been shown that the Golden Rule cannot be universally applied to all patients; it therefore became necessary to find a formula adaptable for each patient.
More informationSupplementary Figure 1. Nature Neuroscience: doi: /nn.4547
Supplementary Figure 1 Characterization of the Microfetti mouse model. (a) Gating strategy for 8-color flow analysis of peripheral Ly-6C + monocytes from Microfetti mice 5-7 days after TAM treatment. Living
More informationSUPPLEMENTARY INFORMATION. Supplementary Figures
SUPPLEMENTARY INFORMATION Supplementary Figures Supplementary Figure 1: Characterization of CerTN-L15 expressed in Arabidopsis roots. a. Ratiometric images of CerTN-L15 in roots under osmotic stress Ratiometric
More informationFig. S1. Upregulation of K18 and K14 mrna levels during ectoderm specification of hescs. Quantitative real-time PCR analysis of mrna levels of OCT4
Fig. S1. Upregulation of K18 and K14 mrna levels during ectoderm specification of hescs. Quantitative real-time PCR analysis of mrna levels of OCT4 (n=3 independent differentiation experiments for each
More informationMII. Supplement Figure 1. CapZ β2. Merge. 250ng. 500ng DIC. Merge. Journal of Cell Science Supplementary Material. GFP-CapZ β2 DNA
A GV GVBD MI DNA CapZ β2 CapZ β2 Merge B DIC GFP-CapZ β2 Merge CapZ β2-gfp 250ng 500ng Supplement Figure 1. MII A early MI late MI Control RNAi CapZαβ DNA Actin Tubulin B Phalloidin Intensity(A.U.) n=10
More informationNature Neuroscience: doi: /nn Supplementary Figure 1. Diverse anorexigenic signals induce c-fos expression in CEl PKC-δ + neurons
Supplementary Figure 1 Diverse anorexigenic signals induce c-fos expression in CEl PKC-δ + neurons a-c. Quantification of CEl c-fos expression in mice intraperitoneal injected with anorexigenic drugs (a),
More informationSupplementary Information. Tissue-wide immunity against Leishmania. through collective production of nitric oxide
Supplementary Information Tissue-wide immunity against Leishmania through collective production of nitric oxide Romain Olekhnovitch, Bernhard Ryffel, Andreas J. Müller and Philippe Bousso Supplementary
More informationSonic hedgehog overexpression regulates the neuroepithelial cells proliferation in the spinal cord of dorsal regions during chicken embryo development
O R I G I N A L A R T I C L E Neuroendocrinology Letters Volume 36 No. 4 2015 ISSN: 0172-780X; ISSN-L: 0172-780X; Electronic/Online ISSN: 2354-4716 Web of Knowledge / Web of Science: Neuroendocrinol Lett
More informationSUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb2294 Figure S1 Localization and function of cell wall polysaccharides in root hair cells. (a) Spinning-disk confocal sections of seven day-old A. thaliana seedlings stained with 0.1% S4B
More informationSupplemental Information. Ciliary Beating Compartmentalizes. Cerebrospinal Fluid Flow in the Brain. and Regulates Ventricular Development
Current Biology, Volume Supplemental Information Ciliary Beating Compartmentalizes Cerebrospinal Fluid Flow in the Brain and Regulates Ventricular Development Emilie W. Olstad, Christa Ringers, Jan N.
More informationCerberus is a feedback inhibitor of Nodal asymmetric signaling in the chick embryo
RESEARCH ARTICLE 2051 Development 134, 2051-2060 (2007) doi:10.1242/dev.000901 Cerberus is a feedback inhibitor of Nodal asymmetric signaling in the chick embryo Ana Teresa Tavares 1, *, Sofia Andrade
More informationSupplementary Figure 1
Supplementary Figure 1 Kif1a RNAi effect on basal progenitor differentiation Related to Figure 2. Representative confocal images of the VZ and SVZ of rat cortices transfected at E16 with scrambled or Kif1a
More informationEl Azzouzi et al., http ://www.jcb.org /cgi /content /full /jcb /DC1
Supplemental material JCB El Azzouzi et al., http ://www.jcb.org /cgi /content /full /jcb.201510043 /DC1 THE JOURNAL OF CELL BIOLOGY Figure S1. Acquisition of -phluorin correlates negatively with podosome
More informationT H E J O U R N A L O F C E L L B I O L O G Y
T H E J O U R N A L O F C E L L B I O L O G Y Supplemental material Courtheoux et al., http://www.jcb.org/cgi/content/full/jcb.200902093/dc1 S1 Figure S2. Visualization of multiple merotelic attachments.
More informationSupplemental Information. Figures. Figure S1
Supplemental Information Figures Figure S1 Identification of JAGGER T-DNA insertions. A. Positions of T-DNA and Ds insertions in JAGGER are indicated by inverted triangles, the grey box represents the
More informationThe left-right axis in the mouse: from origin to morphology
REVIEW 2095 Development 133, 2095-2104 (2006) doi:10.1242/dev.02384 The left-right axis in the mouse: from origin to morphology Hidetaka Shiratori and Hiroshi Hamada* The past decade or so has seen rapid
More informationInfluenza virus exploits tunneling nanotubes for cell-to-cell spread
Supplementary Information Influenza virus exploits tunneling nanotubes for cell-to-cell spread Amrita Kumar 1, Jin Hyang Kim 1, Priya Ranjan 1, Maureen G. Metcalfe 2, Weiping Cao 1, Margarita Mishina 1,
More informationNature Neuroscience: doi: /nn Supplementary Figure 1
Supplementary Figure 1 Subcellular segregation of VGluT2-IR and TH-IR within the same VGluT2-TH axon (wild type rats). (a-e) Serial sections of a dual VGluT2-TH labeled axon. This axon (blue outline) has
More informationSupplementary Figure 1 Lymphocytes can be tracked for at least 4 weeks after
Supplementary Figure 1 Lymphocytes can be tracked for at least 4 weeks after photoconversion by using H2B-Dendra2. 4-5 PPs of H2B-Dendra2 BM chimeras were photoconverted and analyzed 7 days (upper panel)
More informationSupplementary Figure 1: GFAP positive nerves in patients with adenocarcinoma of
SUPPLEMENTARY FIGURES AND MOVIE LEGENDS Supplementary Figure 1: GFAP positive nerves in patients with adenocarcinoma of the pancreas. (A) Images of nerves stained for GFAP (green), S100 (red) and DAPI
More informationNature Neuroscience: doi: /nn Supplementary Figure 1. Distribution of starter cells for RV-mediated retrograde tracing.
Supplementary Figure 1 Distribution of starter cells for RV-mediated retrograde tracing. Parcellation of cortical areas is based on Allen Mouse Brain Atlas and drawn to scale. Thick white curves, outlines
More informationNature Neuroscience doi: /nn Supplementary Figure 1. Characterization of viral injections.
Supplementary Figure 1 Characterization of viral injections. (a) Dorsal view of a mouse brain (dashed white outline) after receiving a large, unilateral thalamic injection (~100 nl); demonstrating that
More informationSupplementary Table 1. List of primers used in this study
Supplementary Table 1. List of primers used in this study Gene Forward primer Reverse primer Rat Met 5 -aggtcgcttcatgcaggt-3 5 -tccggagacacaggatgg-3 Rat Runx1 5 -cctccttgaaccactccact-3 5 -ctggatctgcctggcatc-3
More informationNature Neuroscience: doi: /nn Supplementary Figure 1
Supplementary Figure 1 Atlas representations of the midcingulate (MCC) region targeted in this study compared against the anterior cingulate (ACC) region commonly reported. Coronal sections are shown on
More informationSUPPLEMENTARY INFORMATION
DOI: 0.038/ncb33 a b c 0 min 6 min 7 min (fixed) DIC -GFP, CenpF 3 µm Nocodazole Single optical plane -GFP, CenpF Max. intensity projection d µm -GFP, CenpF, -GFP CenpF 3-D rendering e f 0 min 4 min 0
More informationSUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb2535 Figure S1 SOX10 is expressed in human giant congenital nevi and its expression in human melanoma samples suggests that SOX10 functions in a MITF-independent manner. a, b, Representative
More informationSupplementary Figure S1 (a) (b)
Supplementary Figure S1: IC87114 does not affect basal Ca 2+ level nor nicotineinduced Ca 2+ influx. (a) Bovine chromaffin cells were loaded with Fluo-4AM (1 μm) in buffer A containing 0.02% of pluronic
More informationFigure 4.25: SEM Photo of Hyaluronic Acid Specimen, High Load, 22X
Figure 4.25 shows the specimen from test CS18 at a magnification of 22X. Hyaluronic acid, dissolved at a concentration of 0.375 wt.% in buffered saline solution, was the lubricant in this test, with high
More informationNeocortex Zbtb20 / NFIA / Sox9
Neocortex / NFIA / Sox9 Supplementary Figure 1. Expression of, NFIA, and Sox9 in the mouse neocortex at. The lower panels are higher magnification views of the oxed area. Arrowheads indicate triple-positive
More informationSupplementary Figure 1 Expression of Crb3 in mouse sciatic nerve: biochemical analysis (a) Schematic of Crb3 isoforms, ERLI and CLPI, indicating the
Supplementary Figure 1 Expression of Crb3 in mouse sciatic nerve: biochemical analysis (a) Schematic of Crb3 isoforms, ERLI and CLPI, indicating the location of the transmembrane (TM), FRM binding (FB)
More informationGregor Mendel. What is Genetics? the study of heredity
Gregor Mendel What is Genetics? the study of heredity Gregor Mendel s Peas Pollen: plant s sperm Egg Cells: plants reproductive cells Fertilization: joining of pollen + egg cells develops into embryo in
More informationA new subtype of progenitor cell in the mouse embryonic neocortex. Xiaoqun Wang, Jin-Wu Tsai, Bridget LaMonica & Arnold R.
A new subtype of progenitor cell in the mouse embryonic neocortex Xiaoqun Wang, Jin-Wu Tsai, Bridget LaMonica & Arnold R. Kriegstein Supplementary Figures 1-6: Supplementary Movies 1-9: Supplementary
More informationLong term ex vivo maintenance of testis tissues producing fertile sperm in a microfluidic device
Long term ex vivo maintenance of testis tissues producing fertile sperm in a microfluidic device Mitsuru Komeya 1, 2, Hiroshi Kimura 3, Hiroko Nakamura 3, Tetsuhiro Yokonishi 1,2, Takuya Sato 1, Kazuaki
More informationSupplemental Material
Supplemental Material Supplementary Fig. 1. EETs stimulate primary tumor growth. a) Schematic presentation of genetic and pharmacological tools used to manipulate endogenous EET levels. b) Endothelial
More informationtom tom 24hpf tom tom 48hpf tom 60hpf tom tom 72hpf tom
a 24hpf c 48hpf d e 60hpf f g 72hpf h i j k ISV ISV Figure 1. Vascular integrity defects and endothelial regression in mutant emryos. (a,c,e,g,i) Bright-field and (,d,f,h,j) corresponding fluorescent micrographs
More information(a-r) Whole mount X-gal staining on a developmental time-course of hearts from
1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 Supplementary Figure 1 (a-r) Whole mount X-gal staining on a developmental time-course of hearts from Sema3d +/- ;Ephb4 LacZ/+ and Sema3d -/- ;Ephb4 LacZ/+ embryos.
More informationSUPPLEMENTARY INFORMATION
DOI: 10.1038/ncb2610 Figure S1 FSMCs derived from MSLN CLN transgenic mice express smooth muscle-specific proteins. Beta-galactosidase is ubiquitously expressed within cultured FSMCs derived from MSLN
More informationSUPPLEMENTARY INFORMATION
In the format provided by the authors and unedited. 2 3 4 DOI: 10.1038/NMAT4893 EGFR and HER2 activate rigidity sensing only on rigid matrices Mayur Saxena 1,*, Shuaimin Liu 2,*, Bo Yang 3, Cynthia Hajal
More informationType of file: PDF Title of file for HTML: Supplementary Information Description: Supplementary Figures
Type of file: PDF Title of file for HTML: Supplementary Information Description: Supplementary Figures Type of file: MOV Title of file for HTML: Supplementary Movie 1 Description: NLRP3 is moving along
More informationConserved properties of dentate gyrus neurogenesis across postnatal development revealed by single-cell RNA sequencing
SUPPLEMENTARY INFORMATION Resource https://doi.org/10.1038/s41593-017-0056-2 In the format provided by the authors and unedited. Conserved properties of dentate gyrus neurogenesis across postnatal development
More informationSupporting Online Material for
www.sciencemag.org/cgi/content/full/1171320/dc1 Supporting Online Material for A Frazzled/DCC-Dependent Transcriptional Switch Regulates Midline Axon Guidance Long Yang, David S. Garbe, Greg J. Bashaw*
More informationNature Neuroscience: doi: /nn Supplementary Figure 1. ACx plasticity is required for fear conditioning.
Supplementary Figure 1 ACx plasticity is required for fear conditioning. (a) Freezing time of conditioned and control mice before CS presentation and during CS presentation in a new context. Student s
More informationSupporting Information
Supporting Information Creuzet 10.1073/pnas.0906072106 Fig. S1. Migrating CNC cells express Bmp inhibitors. (A) HNK1 immunolabeling of migrating CNC cells at 7 ss. (B) At this stage, Fgf8 is strongly expressed
More informationVertebrate Limb Patterning
Vertebrate Limb Patterning What makes limb patterning an interesting/useful developmental system How limbs develop Key events in limb development positioning and specification initiation of outgrowth establishment
More informationMore Than 95% Reversal of Left Right Axis Induced by Right-Sided Hypodermic Microinjection of Activin into Xenopus Neurula Embryos
Developmental Biology 221, 321 336 (2000) doi:10.1006/dbio.2000.9666, available online at http://www.idealibrary.com on More Than 95% Reversal of Left Right Axis Induced by Right-Sided Hypodermic Microinjection
More informationSupplemental Data Figure S1 Effect of TS2/4 and R6.5 antibodies on the kinetics of CD16.NK-92-mediated specific lysis of SKBR-3 target cells.
Supplemental Data Figure S1. Effect of TS2/4 and R6.5 antibodies on the kinetics of CD16.NK-92-mediated specific lysis of SKBR-3 target cells. (A) Specific lysis of IFN-γ-treated SKBR-3 cells in the absence
More informationTransient β-hairpin Formation in α-synuclein Monomer Revealed by Coarse-grained Molecular Dynamics Simulation
Transient β-hairpin Formation in α-synuclein Monomer Revealed by Coarse-grained Molecular Dynamics Simulation Hang Yu, 1, 2, a) Wei Han, 1, 3, b) Wen Ma, 1, 2 1, 2, 3, c) and Klaus Schulten 1) Beckman
More informationSupplementary information
Supplementary information 1 Supplementary Figure 1. CALM regulates autophagy. (a). Quantification of LC3 levels in the experiment described in Figure 1A. Data are mean +/- SD (n > 3 experiments for each
More informationSupplementary Figure 1 Madm is not required in GSCs and hub cells. (a,b) Act-Gal4-UAS-GFP (a), Act-Gal4-UAS- GFP.nls (b,c) is ubiquitously expressed
Supplementary Figure 1 Madm is not required in GSCs and hub cells. (a,b) Act-Gal4-UAS-GFP (a), Act-Gal4-UAS- GFP.nls (b,c) is ubiquitously expressed in the testes. The testes were immunostained with GFP
More informationT H E J O U R N A L O F C E L L B I O L O G Y
T H E J O U R N A L O F C E L L B I O L O G Y Supplemental material Lu et al., http://www.jcb.org/cgi/content/full/jcb.201012063/dc1 Figure S1. Kinetics of nuclear envelope assembly, recruitment of Nup133
More informationSupplementary Figure 1. Properties of various IZUMO1 monoclonal antibodies and behavior of SPACA6. (a) (b) (c) (d) (e) (f) (g) .
Supplementary Figure 1. Properties of various IZUMO1 monoclonal antibodies and behavior of SPACA6. (a) The inhibitory effects of new antibodies (Mab17 and Mab18). They were investigated in in vitro fertilization
More informationA genetically targeted optical sensor to monitor calcium signals in astrocyte processes
A genetically targeted optical sensor to monitor calcium signals in astrocyte processes 1 Eiji Shigetomi, 1 Sebastian Kracun, 2 Michael V. Sofroniew & 1,2 *Baljit S. Khakh Ψ 1 Departments of Physiology
More informationDepartment of Cell and Developmental Biology, Vanderbilt University, Nashville, TN
UNC-6/Netrin mediates dendritic self-avoidance Cody J. Smith 1, Joseph D. Watson 1,4,5, Miri K. VanHoven 2, Daniel A. Colón-Ramos 3 and David M. Miller III 1,4,6 1 Department of Cell and Developmental
More informationCapu and Spire Assemble a Cytoplasmic Actin Mesh
Developmental Cell 13 Supplemental Data Capu and Spire Assemble a Cytoplasmic Actin Mesh that Maintains Microtubule Organization in the Drosophila Oocyte Katja Dahlgaard, Alexandre A.S.F. Raposo, Teresa
More informationDermoscopy: Recognizing Top Five Common In- Office Diagnoses
Dermoscopy: Recognizing Top Five Common In- Office Diagnoses Vu A. Ngo, DO Department of Family Medicine and Dermatology Choctaw Nation Health Services Authority Learning Objectives Introduction to dermoscopy
More informationF-actin VWF Vinculin. F-actin. Vinculin VWF
a F-actin VWF Vinculin b F-actin VWF Vinculin Supplementary Fig. 1. WPBs in HUVECs are located along stress fibers and at focal adhesions. (a) Immunofluorescence images of f-actin (cyan), VWF (yellow),
More informationExtracellular vesicles are transferred from melanocytes to keratinocytes after UVA irradiation
Supplementary material; Title; Extracellular vesicles are transferred from melanocytes to keratinocytes after UVA irradiation Authors; Petra Wäster 1, Ida Eriksson 1, Linda Vainikka 1, Inger Rosdahl 2,
More informationZhu et al, page 1. Supplementary Figures
Zhu et al, page 1 Supplementary Figures Supplementary Figure 1: Visual behavior and avoidance behavioral response in EPM trials. (a) Measures of visual behavior that performed the light avoidance behavior
More informationPatterning the Embryo
Patterning the Embryo Anteroposterior axis Regional Identity in the Vertebrate Neural Tube Fig. 2.2 1 Brain and Segmental Ganglia in Drosophila Fig. 2.1 Genes that create positional and segment identity
More informationDevelopmental Biology
Developmental Biology 363 (2012) 348 361 Contents lists available at SciVerse ScienceDirect Developmental Biology journal homepage: www.elsevier.com/developmentalbiology Convective tissue movements play
More informationThe Rho kinase Rock2b establishes anteroposterior asymmetry of the ciliated Kupffer s vesicle in zebrafish
RESEARCH ARTICLE 45 Development 138, 45-54 (2011) doi:10.1242/dev.052985 2011. Published by The Company of Biologists Ltd The Rho kinase Rock2b establishes anteroposterior asymmetry of the ciliated Kupffer
More information