CURRICULUM VITAE. Mobile: ,

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1 CURRICULUM VITAE Name Sex Marital Status Nationality Anthony Kebira Nyamache Male Married Kenyan Contacts Department of Medical Microbiology, Jomo Kenyatta University of Agriculture & Technology Faculty of Science P.O. Box Nairobi Mobile: , Education PhD Medical Virology Institute of infectious diseases & tropical medicine, Jomo Kenyatta University of Agri & Technology, Nairobi Kenya MSc Medical Virology, Institute of infectious diseases & tropical medicine, Jomo Kenyatta University of Agri & Technology, Nairobi Kenya Dissertation Extent of drug resistance mutations among HIV-1 positive drug-naive patients in Kenya BSc Medical Microbiology, 2 nd Class Honour Upper division, Dept of Medical microbiology, Jomo Kenyatta University of Agri & Technology, Nairobi, Kenya

2 Diploma Medical Laboratory Technology, Credit: Mombasa Polytechnic University College, Mombasa, Kenya Koelel High School A levels Jomo Kenyatta University of Agri. & Technology, Department of Medical Microbiology Career Progression July 2005-to date Laboratory Technologist, Department of Botany, Jomo Kenyatta University of Agr. & Technology Duties: Supervision of practical sessions in Medical Microbiology/general microbiology in various techniques, bacterial, fungal and viral isolations. Molecular techniques, PCR amplification, protein separations, gel electrophoresis etc, Development of practical protocols, SOPs, and supervision and maintenance of laboratory inventories, order and procurement of laboratory reagents, supervise monitor and maintenance of laboratory equipments. Training and supervision of other laboratory technologist/technicians, Participate in departmental research activities including co-supervision of undergraduate student s research projects, FTSE/timetabling officer, safety officer etc. Feb 2005-June 2007 HIV/AIDS research assistant, University of Washington/Varsity of Nairobi/Kenyatta National Hospital, Dept of Medical Microbiology HIV/AIDS research/ctl project P.O. Box Nairobi Kenya. Research participation Involved in both basic and clinical trials on HIV/AIDS prevention vertical transmission, assessment of immune responses in both mother and their infants to HIV peptides, Techniques Proliferation assays for infants to various antigens e.g. HIV peptides, Rubella virus, tetanus etc, PBMC isolation from blood, saliva, breast milk and cell counts, freeze cryogenation, thawing of cell cultures, ELISPOT assays and ELISA techniques. Intracellular staining, PCR genotyping for chemokine polymorphisms, Lymphoproliferation assays, RT PCR assay, use of ARMS PCR in HLA biotyping, Viral load estimation by amplicor & Nucleisins kit and Flow cytometry in CD4/CD8 count. Bacterial and fungal isolations by use of specific media and HPLC applications in xenobiotics research. Sep 2003-Feb2005 Research assistant, African Medical Research Foundation P.O Box Nairobi Kenya

3 Involved in routine laboratory diagnosis in hematological, parasitological, clinical chemistry, immunology, and microbiology tests. Use of photometer, flame photomers, bacterial, fungal isolation, ELISA, Viral load, CD4/CD8 counts Quality control on all laboratory tests for AMREF, MALTISER and NASCOP rural-urban clinics, NGOs from southern Sudan project. National Public blood donor unit Nairobi, Kisumu Project and bacterial cultures scheme. Testing and monitoring on HIV and AIDS patients undergoing ARV (antiretroviral) drugs at AMREF-KIBERA CBHC (AMREF/CDC Project) for CD4/CD8 counts, viraloads, LFTs, RFTs and hematological profiles etc. Involved with ARV toxicity and resistance research. Microbiological water quality analysis and monitoring of fields projects : Laboratory incharge, Maximum Médical Centre, P.O. Box 3295 Nairobi Tel Involved in routine diagnosis, hematological, tests using coulter machines, parasitological tests. Clinical chemistry tests using photometer, flame photomers for electrolytes. Microbiological bacterial, fungal isolations and viral diagnosis ELISA, and Immunological or serological etc. Feb2000-Dec 2000: Lab histotechnologist, Nairobi City Mortuary P.O. Box Nairobi Entirely involved with autopsies postmortem tissue processing both legal and normal deaths Apr 1998-Jan 2000: Laboratory technologist, Aga Khan Hospital P.O Box Nairobi, Kenya Duties: Involved in routine diagnosis, hematological test using Maxim, Coulter machines, QBC, parasitological tests and clinical chemistry tests by Hitach machine, photometer, flame photomers for electrolytes, blood gases, drug Analysis, T4 T3, LFTs, RFTs cardiac enzymes, cancer markers, fertility hormones & endocrinology by automated analyzer. Microbiological bacterial, fungal isolations, & viral diagnosis ELISA, western blot electrophoresis, viral load, CD4/CD8 count, RIA-IgM-IgG for Rubella, Toxoplasma, Cytomegallo viruses and Immunological or serological tests tissue processing of biopsies with fewer autopsies etc. Research trainings MSc. Laboratory technical training 2006/2007 Molecular characterization of HIV virus and drug resistances testing; techniques:

4 RNA isolations, Convection PCR RT amplifications targeting gag, pol and env regions of HIV genome. HIV screening by both rapid & ELISA/PCR & cloning. HIV sequencing both direct and clone vector-sequencing approaches Other techniques trained at KEMRI Kenya Viral isolations: preparation of cell cultures and isolation of Sindbis virus, West Nile virus, Rift valley fever, Chikungunya virus from ticks collected from fields by use of mice (Brain inoculation & monitoring) and cell cultures, PCR diagnosis for Human papilloma virus, HIV, ELISA for measles, Rubella virus, poliovirus isolation & sub typing. PCR determination of HIV and sequencing by use of ABI 3100 gene sequencer for HIV subtypes Bioinformatics tools i.e. BLAST, ClustaW, Phylogenetic alignment etc. Aug -Nov 08 3 Months went for Advanced research training on advanced techniques in Molecular characterization of HIV genome at Medical Virology Division, Dept of Pathology faculty of health science, University of Stellenbosch: PCR amplifications, cloning, DNA Sequencing, Phenotypic and genotypic HIV drug resistance testing; Allele specific PCR sequencing, List of written work, publications & presentations BSc project: MSc thesis: A comparative study of the efficacy of generics and original of Norfloxacin against Escherichia coli Extent of drug resistance mutations among HIV-1 positive drug-naive patients in Kenya Others presentations and publication and those in press: 1. Kebira A, P. Ochola; Isolation and antimicrobial susceptibility patterns of pathogenic Escherichia coli causing UTI. Presented & published on at 2 nd university conference proceedings held Nov th Nov 2006 at Jomo Kenyatta University of agricultural and technology Kenya. ISBN no Kebira A., Onyango W., Wasike. D & Matiru V.; A comparative evaluation on broth microdilution, disc diffusion and agar dilution methods on antifungal susceptibility testing of plant extracts. Presented and published at 2 nd university faculty conference proceedings held June13-14 th 2006 at Jomo Kenyatta university of agricultural and technology ISBN no

5 3. Kebira A. & H. Boga, A comparative study of the efficacy of generics and original of Norfloxacin against Escherichia coli Presented and published at 3 rd University faculty conference proceedings held on Nov Kebira A, Waihenya R, Khamadi S.A. Extent of drug resistance mutations among HIV-1 positive drug-naive patients in Kenya submitted and accepted in International AIDS HIV pathogenicity, prevention and treatment conference 2009 Cape Town South Africa on July abstract No. CDC Kebira A, Waihenya R, Khamadi S.A. Primary genotypic drug resistance mutations among selected HIV-1 positive drug-naive populations of Kenya presented and published at inaugural Institute of tropical medicine and infectious diseases post graduate conference th May 2009 at Jomo Kenyatta University of Agricultural and Technology 6. Kebira A, Waihenya R, Khamadi S.A. HIV genetic diversity among drug naïve patients in Kenya submitted for the upcoming International AIDS Vaccine conference Oct 2009 in Paris 7. Kebira A, Waihenya R, Khamadi S.A. Extent of drug resistance mutations among HIV-1 positive drug-naive patients in Kenya. Submitted 8. Kebira A, Waihenya R, Khamadi S.A. HIV genetic diversity among drug naïve patients in Kenya Submitted Workshops/ Research training and seminars attended 4 th -5 th May Introductory course in Bioinformatics, CGRI/ ILRI Nairobi Kenya th March 2009 Science and technology-europe Africa Project Workshop, European Union s 7 th Framework programme for Research, Hilton Hotel Nairobi Kenya 10 th Feb 2009 Emergency First AIDS, training, St John Ambulance Nairobi th July st International workshop on HIV pediatrics, Cape Town South Africa Accepted, & to present a paper on primary genotypic drug resistance mutations among drug naïve populations of Kenya June nd Workshop on Pharmacogenetics of Drug Metabolism Nairobi, Kenya University of Nairobi, Kenya, African institute of science and technology, South Africa Aug 22 nd 08 Real Time PCR and its applications in diagnostic & research Division of Medical virology, University of Stellenbosch, Cape Town South Africa Sept 5 th 08 HIV drug resistance and its implication in clinical practice University of Western Cape, Cape Town South Africa 3 rd Nov 08 Use and application of QiaCube automated RNA/DNA extractions from viruses, bacteria, fungal, PBMCs, Plasma and body fluids. Division of Medical virology, University of Stellenbosch, Cape Town South Africa

6 19 th -24 th Jan 09 Principle of HIV research, application in today s research Workshop by University of Washington, Centre for HIV/AIDS research, Seattle USA & Centre for clinical research, Kenya Medical Research Institute KEMRI Headquarters Kenya Research Awards 2008, HIV Research Trust scholarship award 2009 won an award for best presenter in ITROMID, JKUAT postgraduate conference Research grants 2008 HIV Research Trust scholarship research grant USD 17,500 Research grants applications 2009 Applied for young scientist mentorship in molecular biology grants 2009 African doctoral dissertation research fellowship 2009 Kenya -south Africa collaborative research application Current Research participation 1. SDF-1 polymorphism in relation to HIV disease progression in Kenyan population (KEMRI) ongoing 2. CCR2 polymorphism in relation to HIV disease progression in Kenyan population (KEMRI) ongoing 3. Mapping of Molecular characterization of HIV-1circulating subtypes in Kenya (KEMRI) ongoing 4. Global fund in infant diagnosis by PCR ELISA (KEMRI) ongoing 5. Immune responses and side effects in antiretroviral therapy (AMREF) Research interests: HIV evolution, molecular epidemiology, drug resistances and strategies in design for vaccines, diagnostic kits and microbicides in control of viral diseases Other qualifications Computer packages; Ms Word, Ms Excel, Ms power point, Ms Access, SSPS for word, , Internet, Ms. outlook Acrobat reader etc.

7 Research collaborations 1. Division of Medical virology Department of Pathology, University of Stellenbosch, Cape Town South Africa. Prof Susan Engelbretch, Prof Wolfgang Preiser. 2. Center for Virus Research, Kenya Medical Research Institute, KEMRI, Kenya. Point person, Dr Samoel Ashimosi Khamadi, Dr Matilu Mwau Collaborative research interest: HIV genetic diversity & epidemiology, evolution, drug resistances, diagnostic & Vaccine design and immunology Memberships: Kenya Society of Microbiology. International society of AIDS EXTRA CURRICULA ACTIVITIES Dorm captain Koelel High school, P.o Box 151 Gilgil Kenya Committee member college magazine writers club Mombasa Polytechnic University College P.O Box 92420, Mombasa,Kenya 2008 to date Sunday school teacher NPC Buruburu, Nairobi to date Bible study leader NPC Buruburu, Nairobi HOBBIES: Football & Listening to music REFEREES 1. Prof. Hamadi Boga, Prof in Microbiology JKUAT P.o Box Nairobi Mobile (067) Dr Rebecca Waihenya, S. lecturer immunology JKUAT P.o Box Nairobi Mobile (067) Dr. Samoel Ashimosi Khamadi, S.Research scientist Head of HIV laboratories, Kenya Medical research institute (KEMRI) Center for virus research P.o. box Nairobi Kenya mobile