Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Royal Liverpool and Broadgreen University Hospitals NHS Trust Liverpool Clinical Laboratories Clinical Immunology 8 th floor Duncan Building Royal Liverpool University Hospital Prescot Street Liverpool L7 8XP Contact: Angela Parker Tel: +44 (0) Angela.Parker@rlbuht.nhs.uk Website: Testing performed at the above address only DETAIL OF ACCREDITATION Autoimmune Disease Testing Manual slide preparation in conjunction with manufacturer s instructions using: Indirect immunofluorescence microscopy Serum, plasma Anti-Nuclear antibodies Manual indirect immunofluorescence using human epithelia type 2(Hep 2) antibodies, Werfen HEP2 kit I&I-CI-AI-SOP-7 Serum, plasma Anti-Neutrophil Cytoplasmic Antibody(ANCA) screen Manual indirect immunofluorescence, Werfen NOVA Lite ANCA kit I&I-CI-AI-SOP3 Serum, plasma Endomysial antibodies (IgA) Manual indirect immunofluorescence, AESKU monkey oesophagus Assessment Manager: GS2 Page 1 of 14
2 : Manual Enzyme linked Immunosorbent assay (ELISA) Using plate washer Ref: LT-3500 washer serial E3EZC: Serum, plasma Glutamic acid decarboxylase 65 (GAD65) antibodies RSR manual ELISA. Qualitative reporting. I&I-CI-AI-SOP-15. Serum, plasma C1 esterase inhibitor function Quidel MicroVue C1 inhibitor Plus manual ELISA. SOP AIS065 Serum, plasma Haemophilus Influenzae type B antibodies Tetanus Antibody Binding Site manual ELISA. I&I-CI-AI-SOP-13 Binding Site manual ELISA Serum, plasma Intrinsic factor antibody Orgentec manual ELISA. I&I-CI-AI-SOP-12. Serum, plasma Ds DNA quantification screen Farr assay using Trinity Biotech manual ELISA. I&I-CI-AI-SOP-11 Serum, plasma Extractable Nuclear Antigens: Ro La Sm RMP Scl-70 Jo-1 Ro-52. Euroimmune immunoblot kit and Euroimmune EuroBlotOne. Result interpretation using EUROLineScan. I&I-CI-AI-SOP-5 AIS062 Assessment Manager: GS2 Page 2 of 14
3 Serum/plasma Serum, plasma Antibodies to the following antigens: AMA-M2 M2-3E Sp100 PML gp210 LKM-1 LC-1 SLA/LP Ro52 Myositis antibodies: Mi2-alpa and beta TIF1gamma MDA5 NXP2 SAE1 Ku PM-Scl100 Pm-Scl75 Jo-1 SRP PL-7 PL-12 EJ OJ Liver Immunoblot qualitative assay I&I-CI-AI-SOP-5 Euroimmune immunoblot kit and Euroimmune EuroBlotOne, and result interpretation using EUROLineScan. Myositis immunoblot qualitative assay I&I-CI-AI-SOP-5 AIS047 Serum, plasma Detection of Interferon-gamma Interferon-gamma release assay using Qiagen QuantiFERON-TB Gold plus ELISA. Manual. I&I-CI-AI-SOP-8 Assessment Manager: GS2 Page 3 of 14
4 Serum Immunochemistry Paraprotein typing and quantitation Cyclic citrullinated peptide (CCP) antibody Sebia Capillarys capillary zone electrophoresis, Sebia Hydrasys 2 scan gel electrophoresis using Hydragel 9 IF kit. I&I-CI-IC-SOP-8. Phadia 250 EliA fluorescent enzyme sandwich immunoassay. I&I-CI-IC-SOP-3, 1CS096,ICS,ICS099 Phadia 250 EliA fluorescent enzyme sandwich immunoassay. Qualitative reporting. I&I-CI-IC-SOP-3 Ds DNA screen. Extractable nuclear antigen (ENA) screen (Symphony) Rheumatoid factor IgA Tissue transglutaminase Glomerular basement membrane (GBM) antibodies Myeloperoxidase (MPO sensitive) antibody quantitation Proteinase 3 (PR3 sensitive) antibody quantitation IgA tissue transglutaminase Assessment Manager: GS2 Page 4 of 14
5 Serum Immunological examinations for the IgA Albumin Free light chains quantitation Turbidimetry using Optilite analyser and Binding Site Optilite kit I&I-CI-IC-SOP-6 I&I-CI-IC-SOP-6 I&I-CI-IC-SOP-6 Urine Free light chains quantitation Sebia Hydrasys on gel electrophoresis. Serum C1 Est Inhibitor Turbidimetry using Optilite analyser ICS096 ICS098 ICS099 Avian precipitins Phadia 250 Immunocap. IgG Aspergillus Tryptase Lymphocyte markers: CD3 CD4 CD8 CD19 CD56 Flow cytometry using BD FACSCanto ICS 094ICS096 ICS 100 I&I-CI-IC-SOP4 Urine Bence Jones Protein Electrophoresis using Sebia CZE and Sebia Hydrasys 2 scan gel electrophoresis using Hydragel kit. I&I-CI-IC-SOP-7 ICS 086 ICS087 ICS032 Assessment Manager: GS2 Page 5 of 14
6 Serum Monoclonal protein identification Immunofixation using Sebia Hydrasys 2 Scan and Hydragel 9IF Sebia kit Quantitation: using albumin and delimiting the paraprotein peak I&I-CI-IC-SOP-7 ICS066 Serum IgE total Phadia 250 Immunocap. Serum Allergy related testing Almond nut Alternia alternata Alpha-amylase Apple Aspergillus Fumig Avian antibodies Bee Venom Beef Banana Brazil Nut Budgie Droppings Morphine Pholcodine Ampicilloyl Amoxicilloyl Gelatin Chlorhexidine Candida Albicans Dog Dander Cat Epithelium E1 Cheddar cheese Coconut Chicken Cashew nut Cod Cladosporium Herb Cotton fibre Phadia 250 Immunocap Assessment Manager: GS2 Page 6 of 14
7 Crab Cotton seed D.farinae Dog Dander IgE Canary bird dropping Parrot feathers Pigeon feathers Ferret epithelium Chicken Droppings Chicken, S.proteins Guinea pig epith. Goose feathers Budgerigar droppings Budgerigar feathers Budgie serum protein Goat epithilium Sheep epithelium Chicken feathers Duck feathers Egg White Buckwheat Pea Lima bean Pecan nut Pistachio Trout Clam Lemon Grapefruit Pineapple Blackberry Spinach Lettuce Cabbage Paprika Tea Pumpkin Assessment Manager: GS2 Page 7 of 14
8 ngal d 2 Ovalbumin ngal d 1 Ovomucoid Beetroot Lentil Apricot Cherry Cucumber Egg Guar, Guar Gum Pine Nut Plaice Plum Grape Broccoli Green Pepper Caraway Cardamon Basil Fennel Black pepper Oregano Turkey meat Red Kidney Bean Blueberry Date Oyster Cauliflower Papaya Carob (E410) Sweet chestnut Mandarin Langust Fenugreek Sardine (Pilchard) Carrot Anchovy Rape seed Coriander Assessment Manager: GS2 Page 8 of 14
9 Garlic Crayfish Red Currant Watermelon Orange Mint Linseed Lupin Seed Cochineal extract Cranberry Raspberry Sage Macadamia Nut Lychee rpen a 1 Tropomyosin rara h8 (Peanut) rgly m4 (Soy) Blue mussel Tuna Salmon Omega-5 gliadin rpru p1 (rpeach) Haddock rpru p3 (rpeach) rara h1 (Peanut) rara h2 (rpeanut) rara h 3 Peanut rcor a8 (Hazel nut) rara h9 (Peanut) rcor a1 (Hazel nut) ngly m 5, Soy ngly m 6 Glycinin rcor a14 (Hazel nut) Strawberry ncor a9 (Hazel nut) rjug r 1 Walnut rjug r 3 LTP, Walnut rana o 3 Cashew nut Assessment Manager: GS2 Page 9 of 14
10 Cocksfoot Pollen Onion Rye Chub mackerel Sweet Potato Pacific squid Octopus Barley Oat Egg yolk nbos d4(lactalbumin) nbos d 5 (Milk) Casein Gluten Maize(corn) Lobster Kiwi Parsley Melon Mustard Rice Mango Chocolate (cacao) Pear Peach Avocado Gliadin Feathers (Fowl) Feathers (Tropical) Mixed nuts Mixed fish Mixed cereal foods IgG Anti-Aspergillus Budgerigar droppings Budgerigar feathers Budgerigar mixture Pigeon mixture Pigeon feathers Assessment Manager: GS2 Page 10 of 14
11 Cows Milk Timothy Grass Pollen Pigeon serum Grass pollens (GX1) Grass pollens (GX3) Hazel tree pollen House Dust Mite D1 Hamster epithelium Hazel Nut Horse Dander White faced hornet Horse fly Bumblebee rapi m1 (Honey bee) rves v5(common Wasp) rves v1(common Wasp) Yellow hornet Cockroach Fire ant Mosquito European hornet European Paper Wasp Moth Isocyanate Maxatase Savinase Castor bean Isocyanate TDI Isocyanate MDI Isocyanate HDI Ethylene oxide Phthalic anhydride Formaldehyde Cotton seed Sunflower Latex PehryChrysogenum Cheese, mould type Assessment Manager: GS2 Page 11 of 14
12 Green bean Mutton Mixed Moulds MUXF3 CCD, Bromelain Alpha-Galactose Seminal fluid Pigeon droppings Penicilloyl G Penicilloyl V Peanut Potato Pork Rat epithilium Rabbit epithelium Cefaclor Chinchilla Gerbil epithelium Mackerel Mushroom Cinnamon Coffee Poppy seed Pumpkin seed Vanilla Honey Squid Clove Ginger Chilipepper Curry (Santa Maria) Nutmeg Passion fruit Goat milk Halibut Lime Hake Chickpea Swordfish Assessment Manager: GS2 Page 12 of 14
13 Hop (fruit cone) Sole Scallop Allspice Olive Elder Pine Sacc. Cerevisiae Silver Birch Pollen Sesame seed Shrimp Acarus siro Lepidoglyphus destr. Tyrophagus putresc. Soya Bean Suxamethonium Box elder Maple leaf sycamore Acacia rbet v1 (Birch) rbet v2 (Birch) European ash American Beech Olive Tree Pollen Tomato Mixed tree pollens Tree mix Common ragweed Nettle Rape Sunflower Giant ragweed Mugwort Plantain pollen Wasp Venom White bean Wheat Assessment Manager: GS2 Page 13 of 14
14 Mixed weed pollens Walnut END Assessment Manager: GS2 Page 14 of 14
Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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