Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Oxford University Hospitals NHS Foundation Trust The Churchill Hospital Department of Immunology Old Road Headington OX3 7LE Contact: Kamini Mistry Tel: +44 (0) Testing performed at the above address only Website: DETAIL OF ACCREDITATION purpose of clinical diagnosis /Plasma ELIA assay using in house Myeloperoxidase antibodies Proteinase 3 antibodies Glomerular basement membrane antibodies CCP antibodies Anti- Cardiolipin IgG anti-beta-2 Glycoprotein IgG Extractable nuclear antigen antibody screen Extractable nuclear antigen antibody profile (including antibodies to RNP70, U1-snRNP, Sm, SSA (Ro), SSB (La), Scl-70, CENP, Jo-1, Ro52 and Ro60) Total IGE Tryptase Thyroid peroxidase abs Specific IGE abs t2 Alder (Grey) Pollen f20 Almond c6 Amoxicilloyl f313 Anchovy f49 Apple f237 Apricot m3 Aspergillus Fumigatus f262 Aubergine SOP Immunology In conjunctions with manufacturer s Phadia ImmunoCAP 250 Assessment Manager: JG4 Page 1 of 13
2 purpose of clinical diagnosis /Plasma ELIA assay using in house Specific IGE abs SOP Immunology In conjunctions with manufacturer s f96 Avocado f92 Banana Phadia ImmunoCAP 250 f6 Barley f269 Basil i1 Bee venom (Honey) f27 Beef t3 Birch (Common Silver) f211 Blackberry f280 Black pepper f37 Blue Mussel f18 Brazil Nut f11 Buckwheat m5 Candida albicans f31 Carrot f202 Cashew nut e1 Cat (& dander) f85 Celery f81cheese (cheddar type) f82 Cheese (Mold type). f242 Cherry f299 Chestnut Sweet f309 Chick Pea f83 Chicken meat f279 Chili pepper c8 Chlorhexidine f93 Chocolate (Cacao) f207 Clam f36 Coconut f3 Cod f221 Coffee w1 Common Ragweed f317 Coriander f8 Corn (Maize) f2 Cow's Milk f23 Crab f244 Cucumber f289 Date e5 Dog dander f1 Egg White i75 European hornet venom Assessment Manager: JG4 Page 2 of 13
3 purpose of clinical diagnosis /Plasma ELIA assay using in house Specific IGE abs SOP Immunology ex71 feathers of goose, chicken, duck & turkey ex72 feathers of budgie, finch, canary, parakeet, parrot e217 Ferret epithelium f47 Garlic c74 Gelatin (Bovine) f270 Ginger f79 Gluten f300 Goats Milk f259 Grape g6 Grass (Timothy) gx1 Grass pollen mix e6 Guinea Pig epithelium f42 Haddock e84 Hamster epithelium f17 Hazelnut f247 Honey e3 Horse dander d1 House Dust Mite k77 Isocyanate HDI k76 Isocyanate MDI k75 Isocyanate TDI f84 Kiwi f88 Lamb ( Mutton) k82 Latex f208 Lemon f235 Lentil f215 Lettuce f306 Lime f333 Linseed f80 Lobster f335 Lupin Seed f345 Macadamia nut f206 Mackerel f91 Mango f87 Melon fx1 Mixed Nuts (almond, brazil nut, peanut, hazel nut & coconut) i71 Mosquito In conjunctions with manufacturer s Phadia ImmunoCAP 250 Assessment Manager: JG4 Page 3 of 13
4 purpose of clinical diagnosis /Plasma ELIA assay using in house Specific IGE abs SOP Immunology mx1- Mixed Moulds ( Penicillium Chrysogenum, Cladosporum herbarum, Aspergillus fumigatis, Alternaria alternata) e72 Mouse Urine Protein w6 Mugwort f212 Mushroom f89 Mustard f7 Oat f342 Olive (black) f48 Onion f33 Orange f290 Oyster f86 Parsley f12 Pea f95 Peach f13 Peanut f94 Pear f201 Pecan nut c1 Penicilloyl G c2 Penicilloyl V e215 Pigeon feathers f253 Pine Nut f210 Pineapple f203 Pistachio f255 Plum f224 Poppy seed f26 Pork f35 Potato f225 Pumpkin e82 Rabbit epithelium w203 Rape f316 Rape seed i208 r Api m 1 Phospholipase A2, Honey bee f343 Raspberry e74 Rat Urine Proteins f9 Rice i211 rves v 1 Phospholipase A1, Common wasp In conjunctions with manufacturer s Phadia ImmunoCAP 250 Assessment Manager: JG4 Page 4 of 13
5 purpose of clinical diagnosis /Plasma ELIA assay using in house Specific IGE abs SOP Immunology In conjunctions with manufacturer s i209 rves v 5 Common wasp f5 Rye Phadia ImmunoCAP 250 f41 Salmon f338 Scallop o70 Seminal fluid f10 Sesame seed f24 Shrimp f14 Soy bean f214 Spinach f58 Squid (pacific) k84 Sunflower seed c202 Suxamethonium f218 Sweet pepper/paprika f44 Strawberry f25 Tomato tx8 Tree pollen mix (tx8) (Acer negrundo, Betula Verrucosa, Corylus Avellana, Quercus alba, Plantus acerifulia f204 Trout f40 Tuna f256 Walnut i3 Wasp venom (common) f4 Wheat f15 White Bean f45 Yeast Peanut components F422 r Ara h 1 Peanut F423 r Ara h 2 Peanut F424 r Ara h 3 Peanut F352 r Ara h 8 PR-10 Peanut F427 r Ara h 9 LTP Peanut Hazelnut components F428 rcor a 1 PR-10 Hazelnut F425 rcor a 8 LTP Hazelnut F440 ncor a 9 Hazelnut F439 rcor a 14 Hazelnut Assessment Manager: JG4 Page 5 of 13
6 purpose of clinical diagnosis /Plasma ELIA assay using in house Specific IGE abs SOP Immunology Egg components F233 ngal d 1 Ovomucoid Egg F232 ngal d 2 Ovalbumin Egg Soy components F353 rgly m 4 PR-1o Soy F431 ngly m 5 B conglycinin Soy F432 ngly m 6 Glycinin Soy Wheat components F416 rtri a 19 Omega-5 Gliadin F433 rtri a 14 LTP Wheat Birch components T215 rbet v 1 PR-10 Birch T216 rbet v 2 Profillin Birch T220 rbet v 4 Birch T225 rbet v 6 Birch In conjunctions with manufacturer s Phadia ImmunoCAP 250 Neuroimmunology and Radioimmunoassay (RIA) using Wallac Wizard 1470 automatic Gamma Counter and In-house documented procedures based on equipment manuals as : Acetylcholine receptor antibody immunology 116 Muscle specific Kinase antibody immunology 118 /CSF Voltage Gated potassium Channel antibody immunology 153 /Plasma/CSF NMDA receptor antibodies (Fixed Cell) Manual Indirect immunofluorescence using fixed transfected cell lines using in house SOP immunology 1234 in conjunction with kit instructions using: Euroimmun Assessment Manager: JG4 Page 6 of 13
7 /Plasma /CSF /Plasma /Plasma /Plasma /Plasma /Plasma CSF /Plamsa purpose of clinical diagnosis IgG IgA IgM Total protein Complement C3 Complement C4 Alpha 1 anti-trypsin Ceruloplasmin β2-microglobulin Rheumatoid factor Albumin free light chains IgG subclasses IgG1 IgG2 IgG3 IgG4 Hevylite IgGk IgGλ IgAk IgAλ IgMk IgMλ Monoclonal immunoglobulin proteins (Quantification and identification) Immunochemistry Protein quantitation by turbidimetry on Abbott c4000 Architect Immunochemistry analyser using SOP immunology Immunochemistry Protein quantitation by turbidimetry using in house using SOP immunology 761 In conjunction with manufacturer s Binding Site SPAplus analyser Capillary zone electrophoresis and Immunodisplacement (for monoclonal band identification) using in house SOP immunology 1017 In conjunction with manufacturer s Helena V8 analyser Assessment Manager: JG4 Page 7 of 13
8 Urine, urine purpose of clinical diagnosis Monoclonal immunoglobulin proteins (Bence-Jones proteins) Monoclonal immunoglobulin proteins (identification via immunofixation) Protein electrophoresis using in house SOP immunology 1014 In conjunction with manufacturer s Helena SAS3&4 Protein Immunofixation using in house SOP immunology 1013 (serum) SOP immunology 1015 (urine) SOP immunology 1016 (serum and urine combined) In conjunction with manufacturer s Helena SAS 3&4 using C3 Nephritic factor Protein Immunofixation using in house SOP immunology 1042 In conjunction with manufacturer s Helena SAS 3&4 using Detection of the following using: Isoelectric focusing (IEF) using Sebia Hydrasys in conjunction with in house and CSF IgG oligoclonal bands SOP immuno 64 Alpha 1 anti-trypsin phenotyping SOP immunology 192 Detection of the following using: CH100 Classical complement pathway function AP100 Alternative complement pathway function In-house manual haemolytic assay, measured on Shimadzu UV spectrophotometer using: SOP immuno 67 In-house manual haemolytic assay, measured on Shimadzu UV spectrophotometer using: SOP immuno 67 Assessment Manager: JG4 Page 8 of 13
9 purpose of clinical diagnosis Detection of the following using: C1 esterase inhibitor function Chromogenic assay from Technochrom kit measured on Shimadzu UV spectrophotometer using: SOP immuno 72 C1 esterase inhibitor level Manual Radial immunodiffusion assay from Binding Site - using SOP immunology 475 Cryoglobulin detection, measurement and typing Electrophoresis platform used for typing of croglobulin by immunofixation using: Helena SAS3 In conjunction with in house procedure: SOP immunology 1049 Automated or Manual slide preparation in conjunction with manufacturer s Werfen Quantalyser followed by Indirect Immunofluorescence microscopy using in house Anti-Nuclear antibodies SOP immuno 77 Anti-Neutrophil Cytoplasmic antibodies SOP immuno 78 Anti-Endomysial antibodies (IgG) SOP immuno 88 Anti-Liver/Kidney/Stomach antibodies SOP immuno 76 (GPC, mitochondrial, smooth muscle and LKM) Anti-Endomysial antibodies (IgA) SOP immuno 79 Anti-Ovarian antibodies SOP immuno 82 Anti-Adrenal antibodies SOP immuno 80 Anti-Skin antibodies SOP immuno 84 Anti-Islet cell antibodies SOP immuno 83 Anti-Striated Muscle antibodies SOP immuno 85 /CSF Anti-Paraneoplastic antibodies SOP immuno 114 Assessment Manager: JG4 Page 9 of 13
10 /CSF purpose of clinical diagnosis Anti-Myositis antibodies (Mi2, Ku, PM-Scl 100, PM-Scl 75, Jo-1, SRP, PL-7, PL-12, EJ, OJ Anti-autoimmune liver disease antibodies including (M2, M2-3E(BPO), Sp100, gp210, LKM-1, LC-1, SLA/LP and PML) Anti-Paraneoplastic antibodies (including Hu, Yo, Ri, Ma1, Ma2, CV2/CRMP-5, Amphyphisin, Tr) Immunoblot Manual Immunoblot method from Euroimmun, read and analyser by Euroimmun scanner using: SOP immunology Manual Immunoblot method from Euroimmun, read and analyser by Euroimmun scanner using: SOP immunology Manual Immunoblot method from Euroimmun, read and analyser by Euroimmun scanner using: SOP immunology 115 Anti- tissue Transglutaminase IgA Chemiluminescence immunoassay using in house SOP immunology 791 In conjunction with manufacture s Automated Werfen Bioflash Chemiluminescent Analyser EDTA Blood EDTA Blood EDTA Blood Lymphocyte subset enumeration (Total T cells, CD4 T cells, CD8 T cells, CD19 B cells and NK cells) B cell memory markers (CD19, CD27, CD21, CD38, IgD and IgM T cell memory markers (CD3, CD4, CD27, CD28, CD45RO, CD45RA, CCR7, CD31, CD25, CD127, CXCR5) Flow cytometry using in house procedures and manufacturer s instructions for staining and acquisition and analysis: SOP immunology CHI-SOP-CI19 BD multitest Becton Dickinson SPA III Becton Dickinson FACScanto II SOP immunology Becton Dickinson FACScanto II SOP immunology 973 Becton Dickinson FACScanto II Assessment Manager: JG4 Page 10 of 13
11 EDTA Blood purpose of clinical diagnosis Extended T cell phenotyping (CD3, CD25, CD45, HLA-DR, HLA A+B+C, TCR αβ, TCR γδ ) Flow cytometry using in house procedures and manufacturer s instructions for staining and acquisition and analysis: SOP immunology Becton Dickinson FACScalibur EDTA Blood Double negative alpha/beta T cells SOP immunology Becton Dickinson FACScanto II Heparin Blood T cell proliferation SOP immunology Becton Dickinson FACScalibur EDTA Blood Neutrophil function test SOP immunology 42317Becton Dickinson FACScalibur Becton Dickinson FACScanto II EDTA Blood Toll like receptor (TLR4 and TLR7/8) function SOP immunology Becton Dickinson FACScalibur Becton Dickinson FACScanto II using CD62 ligand shedding Heparin Blood TB specific T cell response Interferon-gamma ELISpot using in house documented procedure: SOP immuno 145 In conjunction with manufacture s Oxford Immunotec kit Quantiferon TB Gold blood collection tubes or heparin whole blood TB specific T cell response Interferon-gamma release assay using in house documented procedure: SOP immuno 149 in conjunction with manufacturer s QuantiFERON-TB gold ELISA Kit, Qiagen Multiskan FC plate reader, ThermoFisher Assessment Manager: JG4 Page 11 of 13
12 / CSF purpose of clinical diagnosis Pneumococcal serotype antibodies (S1, S3, S5, S4, S6b, S7f, S9v, S14, S18c, S19a, S19f, S23f) Anti glutamic acid decarboxylase antibody Thyroid Stimulating Hormone receptor antibody In-house Luminex method using in house documented procedure: SOP immunology 358 In conjunction with manufacturer s Luminex machine Enzyme-Linked immunosorbent assays (ELISA) using manufacture s DS2 automated ELISA analyser, in conjunctions with in house RSR Ltd Sandwich ELISA performed manually or on the DS2 SOP immunology 260 RSR Ltd ELISA - performed manually or on the DS2 - SOP immunology 261 /Plasma dsdna antibody Phadia varelisa dsdna ELISA performed on the DS2 SOP immuno 94 Intrinsic factor antibody Werfen ELISA performed on the DS2 SOP immuno 98 Infliximab level TheraDiag ELISA performed on the DS2 - SOP immunology 1461 anti-infliximab antibody TheraDiag ELISA performed on the DS2 SOP immunology 1462 Adalimumab level TheraDiag ELISA performed on the DS2 SOP immunology 844 Assessment Manager: JG4 Page 12 of 13
13 purpose of clinical diagnosis Enzyme-Linked immunosorbent assays (ELISA) using manufacture s DS2 automated ELISA analyser, in conjunctions with in house Anti-Adalimumab antibody TheraDiag ELISA performed on the DS2 SOP immunology 1460 Tetanus antibodies Binding site ELISA performed on the DS2 SOP immunology 1560 Haemophilus Influenza B antibodies Binding site ELISA performed on the DS2 using SOP immuno 101 Pneumococcal antibodies Binding site ELISA performed on the DS2 using SOP immunology 1473 / plasma / CSF Ganglioside abs, GM1 and GQ1B In-house manual ELISA SOP immuno 99 Anti Myelin associated glycoprotein ab Buhlmann Laboratories ELISA performed manually SOP immuno 100 GBM/ANCA SCREEN for MPO, PR3 and GBM antibodies WIESLAB - Euro-Diagnostica - ELISA performed manually SOP immuno 97 END Assessment Manager: JG4 Page 13 of 13
Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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