Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK

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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Issue No: 001 Issue date: 26 March 2017 Immunology Contact: Vikki Banton Pathology Department Tel: +44 (0) Russells Hall Hospital Fax: +44 (0) Pensnett Road Dudley Website: dudleygroup.nhs.uk DY1 2HQ Testing performed at the above address only DETAIL OF ACCREDITATION Serum Detection of: Indirect Immunofluorescence assay using Quanta-lyser 240 slide processor with specific Serum Serum Anti-Nuclear Antibodies Anti Neutrophil Cytoplasmic antibodies Detection of organ specific autoantibodies Pancreatic Islet antibodies Adrenal antibodies Crithidia antibodies Liver antibodies: Mitochondrial antibodies Smooth Muscle antibodies Liver Kidney Microsomal antibodies Gastric Parietal Cell antibodies Epithelial antibodies Endomysial antibodies Paraneoplastic antibody screen SOP/I/067 SOP/I/001 Manual indirect immunofluorescence assay using : INOVA slides with specific reference to : SOP/I/003 INOVA slides with specific reference to : SOP/I/002 Werfen / Innova slides with specific SOP/I/003 SOP/I/107 Serum Detection of functional antibodies: Manual ELISA procedures using binding site kits with specific HIB Pneumococcus SOP/I/084 SOP/I/083 Assessment Manager: JG4 Page 1 of 9

2 Serum Detection of: Enzyme Immunoassays using the Thermofisher Immunocap 250 instrument with specific reference to SOP/I/075, 066, 005, 096, 106, 007, 072, 006, 050 dsdna abs Intrinsic Factor abs PR3 / MPO antibodies CCP antibodies ACA IgG antibodies B2GP1 antibodies GBM antibodies ENA screen ttg antibodies (A and G) Respiratory IgG antibodies: Aspergillus Pigeon Serum Protein M Faeni T Vulgarins SOP/I/100 Tryptase SOP/I/087, 073 Serum Detection of Extractable Nuclear Antigen specificities (ENA): Paraneoplastic abs Systemic Sclerosis Myositis Automated method using Euroimmun Blotmaster instrument dot blot kit Euroimmun ENA profile with specific SOP/I/022 SOP/I/110 SOP/I/113 SOP/I/112 Serum Detection of Cryoglobulin Manual precipitation method including immunofixation with specific reference to SOP/I/048 Assessment Manager: JG4 Page 2 of 9

3 Cellular investigations: Flow cytometry using Beckman Coulter FC500 and BC kits with specific EDTA samples CD3, 4 & 8 panel T,B, NK panel CD34 panel Euroclass - B cell class switching HLA B27 SOP/I/024 SOP/I/101 SOP/I/071 SOP/I/108 SOP/I/070 Serum Detection of: Electrophoresis using Sebia Hydrasys gel electrophoresis system with specific Serum EPS Serum IMF Paraprotein quantification SOP/I/021 SOP/I/020 SOP/I/080 Serum C1 Esterase inhibitor Radial Immunodiffusion method using Binding site kit with specific SOP/I/047 Assessment Manager: JG4 Page 3 of 9

4 allergens: SP IGE TO A-LACTALBUMIN ALMOND ALT.TENUIS IGE AMOXICILLOYL AMPHICILLOYL Anchovy Ano3 cashew component APPLE APPLE COMPONENT (rmal d1) APRICOT ARA H1 Peanut Component ARA2 ARA H3 Peanut Component ARA8 ARA9 ASPERGILLUS (IGE) AUBERGINE AVOCADO BANANA BARLEY WHITE BEAN BEEF Sp IGE to Brazil comp rber e 1 Betv 1 PR-10 (birch component) Betv2 Profilin birch component BIRCH TREE CAGED BIRDS BLACKBERRY BLACK PEPPER SP IGE TO B-LACTOGLOBULIN BLUEBERRY BOTRITIS CINEREA BRAZIL NUT BROCCOLI BUCKWHEAT BUDGIE DROPPINGS IGE BUDGIE FEATHERS IGE BEE IGE BEE COMPONENT (rapi m1) CACAO Assessment Manager: JG4 Page 4 of 9

5 allergens : CANARY FEATHER CARROT IGE SP IGE TO CASEIN CASHEW CAT CAULIFLOWER CEDAR CELERY IGE CEREAL MIX CHEDDAR TYPE CHEESE CHERRY CHESTNUT CHICKEN FEATHERS CHICKEN MEAT CHICKPEA CHEESE, MOULD TYPE CLADOSPORIUM HERBARUM COCONUT COD COFFEE COR1 COR8 COR9 COR14 CORIANDER CORN COW CHILLI PEPPER CRAB CUCUMBER CURRY (SANTA MARIA MIX) DOG DUCK FEATHERS EGG WHITE EGG YOLK FEATHER FENNEL SEED FOOD MIX FORMALDEHYDE GARLIC Assessment Manager: JG4 Page 5 of 9

6 allergens : GINGER GRAPEFRUIT GLUTEN GOATS MILK GOAT GUINEA PIG GRAIN MIX GRAPE GRASS POLLEN HADDOCK HAMSTER HAZEL NUT HAZEL TREE POLLEN HDM:D farinae HOUSE DUST MITE HONEY Herring HORSE FLY HOPS European Hornet Venom HORSE Yellow Hornet Venom KIWI LAMB LATEX LEMON LENTIL LETTUCE LIME LOBSTER LUPIN SEED Macadamia nut MACE MACKEREL (CHUB) MALT MANGO MELON MILK MILLETT (COMMON) Mint Assessment Manager: JG4 Page 6 of 9

7 allergens : MOULD MIX MORPHINE MOUSE MUGWORT Blue Mussel MUSHROOM MUSTARD Nutmeg NUTS OMEGA 5 GLIADIN OAK OATS OCTOPUS ONION ORANGE OREGANO SP IGE TO OVALBUMIN SP IGE TO OVOMUCOID OYSTER PALM OIL PARROT PEA PEACH / NECTARINE PEACH COMPONENT (rpru p3 LTP) PEAR PECAN NUT PENICILLOYL G&V IGE ABS PIGEON DROPPINGS IGE PIGEON FEATHERS IGE PINEAPPLE PINE NUT PISTACHIO PLUM IGE PEANUT POLLOCK Poppy seed PORK POTATO PUMPKIN SEED Assessment Manager: JG4 Page 7 of 9

8 allergens : RABBIT RAPE SEED FOOD RAPE WEED POLLEN RASPBERRY RAT RED SNAPPER RICE RED KIDNEY BEAN RYE FOOD SALMON SCALLOP SEAFOOD MIX SESAME SEED SHEEP IGE SHEEPS MILK SHRIMP SOYA SWEET PEPPER / PAPRIKA SPICE MIX 1 SPICE MIX 2 SPINACH SQUID STRAWBERRY SUNFLOWER SEED IGE SUXAMETHONIUM (SCOLINE) TOMATO TREE MIX (LATE) TREE EARLY TUNA WALNUT WEED MIX WHEAT WHITE ASH WHITE PINE WASP IGE Assessment Manager: JG4 Page 8 of 9

9 allergens : YEAST (BAKERS) rves v1 Wasp component rves v5 Wasp component Total IgE END Assessment Manager: JG4 Page 9 of 9

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