Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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1 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Clinical Immunology Service The Medical School Vincent Drive Edgbaston Birmingham B15 2TT Contact: Karen Walker Tel: +44 (0) Fax: +44 (0) Website: Testing performed at the above address only DETAIL OF ACCREDITATION HUMAN BODY FLUIDS Immunochemistry Serum : Hitachi COBAS 6000 turbidimeter SOP: COBAS Immunoblobulins (IgG,A,M) IgG subclasses (IgG 1,2,3,4) Serum Free Light Chains Beta 2 Microglobulin Complement components C3/C4 CRP Rheumatoid Factor latex screen Serum Paraprotein Quantitation LAUNCH Microgel using Interlab G26 and Sebia Hydrasys (electrophoresis and densitometry) SOP: G26 DENS SOP: G26SPE SOP: HYDRASYS2 SOP: GEN - MSP ver1.0 Serum/Urine Identification of monoclonal proteins: Serum Cryoglobulins SEBIA Hydrasys (immunofixation of monoclonal immunoglobulins) SOP: HYSYSufix SOP: HYDRASYS2 SOP: CRYO Assessment Manager: DE Page 1 of 14
2 HUMAN BODY FLUIDS (cont d) (cont d) Serum IgD Binding Site radial immunodiffusion SOP: IgD SOP : SRID Serum C1 esterase inhibitor concentration Binding Site radial immunodiffusion SOP : C1 INH Plasma (Citrate) C1 esterase inhibitor function Technochrom C1 inhibitor kit, Pathway Diagnostics. SOP: FC1inh Plasma Viscosity Bensons Viscometer. SOP: VISC Serum IgG Functional antibodies against :- Tetanus toxoid, Pneumococcal serotypes; 1, 3, 4,5, 6B, 7F, 9V, 14, 18, 19A, 19F, 23F, Haemophilus Influenzae b, Meningococcus C LUMINEX multiplex assay SOP: EP-15PG Autoimmune Serology: Serum Quantalyser 160 Indirect Immunofluorescence. SOP: IMF Antinuclear antibodies Centromere antibodies dsdna antibodies (crithidia) Neutrophil cytoplasmic antibodies Adrenal/Ovarian/Testicular antibodies Epidermal antibodies Islet cell antibodies Mitochondrial antibodies Assessment Manager: DE Page 2 of 14
3 HUMAN BODY FLUIDS (cont d) (cont d) Autoimmune Serology Serum Smooth muscle antibodies Quantalyser 160 Indirect Immunofluorescence. SOP: IMF LKM antibodies Gastric parietal cells antibodies Myocardial antibodies Endomysial Neuroantibodies Detection of the following paraneoplastic antibodies: Hu, Yo, Ri, Ma, CV2, amphiphysin, SOX1, Zic4, GAD65 and Tr. MAG antibodies Skeletal Muscle antibodies Serum Detection of liver antibodies : M2/nPDC, gp210, sp100, LKM1, LC1, SLA, f-actin Immunoblot (D-Tek). SOP : Blue Diver Blot Serum dsdna antibodies SOP: DNA Dynex DS2 Immuno Sorbent Assay (ELISA). SOP: DSX ENA antibodies screen ENA antibodies type Cardiolipin antibodies (IgG & M) Cardiolipin antibodies (IgG & M) B2GP1 antibodies (IgG) SOP: ENAS SOP: ENAS SOP: ENAT SOP: CLP SOP: B2GP Assessment Manager: DE Page 3 of 14
4 HUMAN BODY FLUIDS (cont d) Serum (cont d) Autoimmune Serology MPO/PR3 antibodies Anti-C1q autoantibodies M2 antibodies Intrinsic factor antibodies DGP GBM antibodies CCP antibodies Thyroid microsomal (TPO) abs Aspergillus fumigatus Avian (budgerigar, pigeon) Micropolyspora faenii Candida albicans Total IgE Mast cell tryptase allergens: Almond Alternaria alternate Amoxicilloyl Dynex DS2 Immuno Sorbent Assay (ELISA). SOP: DSX SOP: MPO SOP : PR3 SOP: AC1Q SOP: M2 SOP: IF Assessment Manager: DE Page 4 of 14
5 HUMAN BODY FLUIDS allergens: (cont d) Apple Apricot Aspergillus fumigatus Aubergine, eggplant Banana Barley Beef Black pepper Brazil nut Budgerigar feathers Budgerigar serum proteins, feathers and droppings Cacao Canary bird feathers Candida albicans Carrot Cashew nut Cat dander Cheese, cheddar type Cherry Assessment Manager: DE Page 5 of 14
6 HUMAN BODY FLUIDS allergens: (cont d) Chick pea Chicken Chilipepper Cladosporium herbarum Coconut Cod Coffee Common wasp (Yellow jacket) Crab Curry (Santa Maria) Dog dander Egg white Egg yolk Gerbil epithelium Grape Grapefruit Green pepper (unripe seed) Guinea pig epithelium Hamster epithelium Assessment Manager: DE Page 6 of 14
7 HUMAN BODY FLUIDS allergens: (cont d) Hazel nut Honey bee Horse dander House dust mite Kiwi Latex Lemon Lentil Maize, Corn Mango Melon Micropolyspora faenii Milk Mouse serum proteins Mushroom (champignon) Mutton Oat Onion Orange Assessment Manager: DE Page 7 of 14
8 HUMAN BODY FLUIDS Papaya allergens: (cont d) Parrot feathers Passion fruit Pea Peach Peanut Pear Pecan nut Penicilloyl G Penicilloyl V Pigeon droppings Pigeon feathers Pigeon serum proteins, feathers and droppings Pine nut Pineapple Pistachio Plum Pork Potato Rabbit epithelium Assessment Manager: DE Page 8 of 14
9 HUMAN BODY FLUIDS Raspberry allergens: (cont d) Mouse serum proteins Mushroom (champignon) Mutton Oat Onion Orange Papaya Parrot feathers Passion fruit Pea Peach Peanut Pear Pecan nut Penicilloyl G Penicilloyl V Pigeon droppings Pigeon feathers Assessment Manager: DE Page 9 of 14
10 HUMAN BODY FLUIDS allergens: (cont d) Pigeon serum proteins, feathers and droppings Pine nut Pineapple Pistachio Plum Pork Potato Rabbit epithelium Raspberry Rat epithelium, serum proteins and urine proteins Rice Rye Salmon Sesame seed Shrimp Soybean Spinach Strawberry Assessment Manager: DE Page 10 of 14
11 HUMAN BODY FLUIDS allergens: (cont d) Timothy grass Tomato Tuna Walnut Wheat Yeast specific IgE to allergen components: Alpha-gal Thyroglobulin Bovine ngal d 1 Ovomucoid, Egg ngal d 2 Ovalbumin, Egg rapi m 1 Phospholipase A2, Honey bee rara h 1 Peanut rara h 2 Peanut rara h 3 Peanut rara h 8 PR-10, Peanut rara h 9 LTP, Peanut rbet v 1 PR-10, Birch rcor a 1 PR-10, Hazel nut Assessment Manager: DE Page 11 of 14
12 HUMAN BODY FLUIDS specific IgE to allergen components rcor a 8 LTP, Hazel nut rmal d 1 PR-10, Apple rmal d 3 LTP, Apple rpru p 3 LTP, Peach rves v 1 Phospholipase A1, Common wasp rves v 5 Common wasp Mixed trees (Maple, Birch, Oak, Elm, walnut) Mixed weed (Ragweed Mugwort, Oxeye daisy, dandelion, Goldenrod) Mixed nut (Peanut, Hazel nut, Brazil nut, Almond, Coconut) Mixed Seafood (Fish, Shrimp, Blue mussel, Tuna, Salmon) Mixed Cage Birds (Budgerigar, Parrot, Canary, Parakeet, Finch) ttg antibodies (IgA quant) M2 Mitochondrial antibodies GAD antibodies Bioflash Chemiluminescence SOP: BIO-FLASH SOP: ATTG Euroimmune ELISA SOP: M2 SOP: GAD Assessment Manager: DE Page 12 of 14
13 HUMAN BODY FLUIDS Neuroimmunology Serum and CSF Oligoclonal IgG bands In-house Isoelectric focussing and Western blotting. SOP: IEF Serum NMDAR, AMPAR1, AMPAR2, GABA B1R, LGI1, CASPR2 PLA2 Immunofluoresence (Sprinter) SOP: IMFS Body Fluids Tau protein In-house electrophoresis and Western blotting. SOP: Tau Serum Serum Whole Blood (EDTA) Detection of cerebellar antibodies: Hu, Yo, Ri, Ma2, Ma1, CV2, amphiphysin, SOX1, Zic4, GAD65, Tr. Detection of Ganglioside antibodies: (GM1, GM2, GM3, GM4, GD1a, GD1b, GD2, GD3, GT1a, GT1b, sulphatide, GQ1b Immunophenotyping examination activities for the purpose of clinical diagnosis and disease monitoring: Cellular Immunology Quantification of T, B and NK cell subsets Quantification of dual negative CD4/CD8 T cell subsets Immunoblot (Ravo). SOP : Blue Diver Blot Immunoblot (Generic Assays). SOP : Blue Diver Blot Becton Dickinson FACSCanto II (flow cytometry) with specific reference to: SOP: BD FACS Lyse Wash Assistant SOP: CELLPREP SOP: TBNK SOP: Immunodeficiency_FC Whole Blood (Lithium heparin) Neutrophil function tests PhagoBurst kit from BD. SOP: SOP-Neutrophil Burst Assessment Manager: DE Page 13 of 14
14 HUMAN BODY FLUIDS Immunophenotyping examination activities for the purpose of clinical diagnosis and disease monitoring: Cellular Immunology (cont d) Whole Blood (Lithium heparin) T-SPOT TB Oxford Immunotec kit. SOP: TSPOT Whole Blood (Lithium heparin) Quantiferon Quaigen kit. SOP: QFT-PLUS-SOP Serum Whole Blood (EDTA) / Bone Marrow/ CSF / Body Fluids / Lymph Node Whole Blood (EDTA) Quantitative measurement of TNF alpha, VEGF, IL-6, IFN gamma Haemato-oncology immunophenotyping Leukaemia/lymphoma type Paroxysmal nocturnal hemoglobinuria END BioRad Bioplex Kit. Quantitative measurement of cytokines in serum by the Luminex method. SOP: EP-Cytokines Becton Dickinson FACSCanto II (flow cytometry) with specific reference to: SOP: BD FACS Lyse Wash Assistant SOP: CELLPREP SOP: Slide prep and staining SOP: IQC-FC-Clinical SOP: Immunophenotyping clinical reporting SOP: LPD SOP: Acute Leukaemia Screen SOP: B-ALL Screen SOP: T-ALL Screen SOP: AL cytoplasmic SOP: Myeloid screen SOP: AML SOP: PML stain SOP: Myeloma Flow SOP: PNH Assessment Manager: DE Page 14 of 14
Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK
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