Mystic Air Quality Consultants, Inc North Road, Groton, Connecticut

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1 Mystic Air Quality Consultants, Inc. 04 North Road, Groton, Connecticut July 30, 05 Mrs. Ann Burdick New London Public Schools 4 Williams Street New London, CT 0630 Re: Indoor Air Quality and Environmental Fungi (mold) Evaluation, 4 Williams Street, Lower Level Health Dear Ms. Burdick, Mystic Air Quality Consultants, Inc. conducted an indoor air quality survey including sampling for environmental fungi (mold) at the location referenced above on July 4, 05. The survey was conducted by Chris Muller, under the direction of a Certified Industrial Hygienist and Certified Safety Professional. Indoor mold air samples were collected using Allergenco-D cassettes taken at 5 LPM for 5 minutes. The mold samples were analyzed by SanAir Technologies, an AIHA and EMPAT accredited laboratory. The findings of the air sampling for environmental fungi are presented in Enclosure (). Please note that even though all mold spores and particles are potential allergens and sources of infection, there are currently no State or Federal legal standards for mold levels in the air and in or on surfaces. At extremely high levels the mold spores and particles are irritants that could cause eye and respiratory tract irritation with symptoms including congestion, tearing, coughing, etc. The primary purpose of sampling and inspections is to determine if the levels are higher than normal or higher than outside air and/or control samples. To evaluate mold levels we normally compare the total counts (pieces of mold and mold spores) per cubic meter of air for each area with the outside air level. A summary of the mold level findings are presented in the following table: Air Sample Location Mold Counts/M3 Office Veronica Abbott 440 Waiting Room 547 Outside Control Sample 547 Mystic Air Quality is an AIHA Accredited Lab FAX:

2 Mystic Air Quality Consultants, Inc. 04 North Road, Groton, Connecticut The interior sample results collected in the administrative area depict minimal total mold spore counts. Significant mold spore levels are typically regarded as levels 0 times the Outside Control Sample count or counts greater than 5,000/M3. The mold spore levels are typical of normal seasonal conditions. If a thorough cleaning is desired, it is recommended to clean all horizontal and vertical surfaces in the rooms with a vacuum equipped with a High Efficiency Particulate Air (HEPA) filtration system. The cleaning of hard surfaces may be performed with a mild detergent soap or a to 0 bleach solution. Sincerely, 707,..Mer Mark Trotochaud, CSP Mystic Air Quality is an All-IA Accredited Lab FAX

3 SanAir Technologies Laboratory Analysis Report prepared for Mystic Air Quality Consultants Report Date: 7/9/05 Project Name: New London, CT Project #: 4 William St. SanAir ID#: 507 Kivta NVLAP LAB CODE ilvainalic ACCIEMOLPLOMMIN ealsawspas minim DB/695 Certification / 6593 Texas Lkensect Mold Analysis Laboratory License C LA8066 Sank Technologies Laboratory ENCLOSURE! PAGE I OF5

4 SanAit SanAir Technologies Laboratory, Inc. SanAir ID Number Oakbridge Drive, Suite B, Powhatan, VA Toll Free: Fax: Web: Name: Address. FINAL REPORT Mystic Air Quality Consultants Linda Lastella 04 North Road Groton, CT Project Number: P.O. Number: Project Name: 4 William St. New London Schools New London, CT Collected Date: Received Date: Report Date: Analyst: 7/4/05 7/8/05 0:5:00 AM 7/9/05 :0:9 PM Acharya, Uttam Air Cassette Anal sis NO = None Defected SanAir ID Number Analysis Using STL: Sample Number Sample Identification Sample Type Volume Analytical Sensitivity S A O-c 0W 3WS00N3 Background Density Fungal Identification Altemaria species Ascospores Basidiospores Bipolaris/Drechslera Cladosporium species Curvularia species Epicoccum species Rusts Smuts/Msommycetes Total Signature: C Outside Air Cassette - Allergenco-D 75 Liters Count/Ma + 05C Office - Veronica Abbott Air Cassette - Allergenco-D 75 Liters Count/Ma Raw Count Count/NP % Raw Count Count/M, C Waiting Room Air Cassette - Allergenco-D 75 Liters Count/Ma + % Date: 7/9/05 Reviewed Raw Count CountRA, % a,. Date: 7/9/05 Page of

5 laarr SanAir Technologies Laboratory, Inc. 55 Oakbridge Drive, Suite B, Powhatan, VA Toll Free: Fax: Web: Name: Mystic Air Quality Consultants Address: Linda Lastella 04 North Road Groton, CT SanAir ID Number 507 FINAL REPORT Project Number: P.O. Number: Project Name: Collected Date: Received Date: Report Date: 4 William St. New London Schools New London, CT 7/4/05 7/8/05 0:5:00 AM 7/9/05 :0:9 PM ORGANISM DESCRIPTIONS The descriptions of the organisms presented are derived from various reference materials. The laboratory report is based on the data derived from the samples submitted and no interpretation of the data, as to potential, or actual, health effects resulting from exposure to the numbers of organisms found, can be made by laboratory personnel. Any interpretation of the potential health effects of the presence of this organism must be made by qualified professional personnel with first hand knowledge of the sample site, and the problems associated with that site. ALTERNARIA SPECIES - This genus compromises a large number of saprobes and plant pathogens. It is one of the predominate airborne fungal spores indoor and outdoor. Outdoors it may be isolated from samples of soil, seeds, and plants. It is one of the more common fungi found in nature, extremely widespread and ubiquitous. Conidia are easily carried by the wind, with peak concentrations in the summer and early fall. It is commonly found in outdoor samples. It is often found in indoor environments, on drywall, ceiling tiles, in house dust, carpets, textiles, and on horizontal surfaces in building interiors. Often found on window frames. Health Effects: In humans, it is recognized to cause type I and III allergic responses. Because of the large size of the spores, it can be deposited in the nose, mouth and upper respiratory tract, causing nasal septum infections. It has been known to cause Bakers asthma, farmer's lung, and hay fever. It has been associated with hypersensitivity pneumoniti, sinusitis, deratomycosis, onychomycosis, subcutaneous phaeohyphomycosis, and invasive infection. Common cause of extrinsic asthma (immediate-type hypersensitivity: type l). Acute symptoms include edema and bronchiospasms, chronic cases may develop pulmonary emphysema. References: Flannigan, Brian, Robert A. Samson, and J. David Miller, eds. Microorganisms in Home and Indoor Work Environments: Diversity, Health Impacts, Investigation, and Control. London and New York: Taylor & Francis, 00. ASCOSPORES - From the fungal Subphylum Ascomycotina. Ascospores are ubiquitous in nature and are commonly found in the outdoor environment. This class contains the "sac fungi" and yeasts. Some ascospores can be identified by spore morphology, however; some care should be excercised with regard to specific identification. They are identified on tape lifts and non-viable analysis by the fact that they have no attachment scars and are sometimes enclosed in sheaths with or without sacs Ascomycetes may develop both sexual and asexual stages. Rain and high humidity may help asci to release, and dispurse ascospores, which is why during these weather conditions there is a great increase in counts. Health Effects: This group contains possible allergens. BASIDIOSPORES - From the Subphylum Basidiomycotina which contains the mushrooms, shelf fungi, and a variety of other macrofungi. They are saprophytes, ectomycorrhizal fungi or agents of wood rot, which may destroy the structure wood of buildings. It is extremely difficult to identify a specific genera of mushrooms by using standard culture plate techniques. Some basidiomycete spores can be identified by spore morphology; however, some care should be exercised with regard to specific identification. The release of basidiospores is dependant upon moisture, and they are dispersed by wind. Health Effects: Many have the potential to produce a variety of toxins. Members of this group may trigger Type I and III fungal hypersensitivity reactions. Rarely reported as opportunistic pathogens. BIPOLARISIDRECHSLERA - Found on grasses, grains, various plants, and decaying food. May grow in semi-dry environments. Some species are found in indoor environments. Because of the microscopic similarities between the two genera, they are grouped together on non-viable analyses. Health Effects: Can occasionally cause comeal infection of the eye. This group of fungi constitutes the most commonly reported causes of allergic fungal sinusitis. They produce type I fungal hypersensitivity in humans. References: St-Germain, Guy, and Richard Summerbell. Identifying Filamentous Fungi: A Clinical Laboratory Handbook. Califomia: Star Publishing Co., 996. CLADOSPORIUM SPECIES - The most commonly identified outdoor fungus. The outdoor numbers are reduced in the winter and are often high in the summer. Often found indoors in numbers less than outdoor numbers. It is commonly found on the surface of fiberglass duct liner in the interior of supply ducts. A wide variety of plants are food sources for this fungus. It is found on dead plants, woody plants, food, straw, soil, paint and textiles. Often found in dirty refrigerators and especially in reservoirs where condensation is collected, on moist window frames it can easily be seen covering the whole painted area with a velvety olive green layer. Health Effects: It is a common allergen. It can cause mycosis. Common cause of extrinsic asthma (immediate-type hypersensitivity: type l). Acute symptoms include edema and bronchiospasms, chronic cases may develop pulmonary emphysema. Illnesses caused by this genus can include phaeohyphomycosis, chromoblastomycosis, hay fever and common allergies. References: Flannigan, Brian, Robert A. Samson, and J. David Miller, eds. Microorganisms in Home and Indoor Work Environments: Diversity, Health Impacts, Investigation, and Control. London and New York: Taylor & Francis, 00. Page of ENCLOSURE / PAGE 3 OF.<

6 -5Sai TAir SanAir Technologies Laboratory, Inc. 55 Oakbridge Drive, Suite B, Powhatan, VA Toll Free: Fax: Web: iaq sanair.com Name: Address: Mystic Air Quality Consultants Linda Lastella 04 North Road Groton, CT SanAir ID Number 507 FINAL REPORT Project Number: P.O. Number: Project Name: Collected Date: Received Date: Report Date: 4 William St. New London Schools New London, CT 7/4/05 7/8/05 0:5:00 AM 7/9/05 :0:9 PM ORGANISM DESCRIPTIONS The descriptions of the organisms presented are derived from various reference materials. The laboratory report is based on the data derived from the samples submitted and no interpretation of the data, as to potential, or actual, health effects resulting from exposure to the numbers of organisms found, can be made by laboratory personnel. Any interpretation of the potential health effects of the presence of this organism must be made by qualified professional personnel with first hand knowledge of the sample site, and the problems associated with that site. CURVULARIA SPECIES - Curvularia is found on plant material and is considered a saprobe. It has also been isolated from dust samples and from wallpaper. Health Effects: It has been reported to cause type I hypersensitivity and to be a cause of allergic fungal sinusitis. It may cause corneal infections, mycetoma and infections in immune compromised hosts. References: De Hoog, G.S., J. Guarro, J. Gene, and M.J. Figueras. Atlas of Clinical Fungi, nd Edition. The Netherlands: CBS, 000. EPICOCCUM SPECIES - It is found in plants, soil, grains, textiles, and paper products. Frequently isolated from air and occasionally occurs in house dust. Is a saprophyte and considered a weakly parasitic secondary invader of plants, moldy paper and textiles. Epicoccum is usually isolated with either Cladosporium species or Aureobasidium species. Health Effects: A common allergen. It also has the potential to produce type I fungal hypersensitivity reactions. References: Flannigan, Brian, Robert A. Samson, and J. David Miller, eds. Microorganisms in Home and Indoor Work Environments: Diversity, Health Impacts, Investigation, and Control. London and New York: Taylor & Francis, 00. RUSTS - From the group Uredinales, called Rusts due to the color of the spores, which are known for causing disease in plants. SMUTS/MYXOMYCETES - Smuts and Myxomycetes are parasitic plant pathogens. They are typically grouped together due to their association with plants, the outdoors and because they share similar microscopic morphology. Heafth Effects: Can produce type I fungal hypersensitivity reactions. References: Martin, G.W., C.J. Alexopoulos, and M.L. Farr. The Genera of Myxomycetes. Iowa City, Iowa: University of Iowa Press, 983. Page of ENCLOSURE I PAGE q OF5

7 sanair recnnoiogies Laboratory, Int 55 OakOndge Dnve, Suite B - Powhatan, VA / / Fax wwksanaircorri Company: Mystic Air Address: 04 Quality Consultants North Road City, State, zip: Groton, Date Collected: Sam las Coleded_By. C \MA. k.-/4-tt.sample Types AC Air Cassette T B Se Tape Bulk Swab AP B Air Plate Bulk S W 0 Swab Water Dust Microbiology Chain of Custody Project Number 4 Prolect Name: CT anAlr 0 Number WWI IAbV St, Nisp..z (0.040,..:4, t _(/ ppm.t pews is t (T7)4-Ls Fa P.O. Number PUGu3 LO4O4/ 4 C Analysis Types Turn Around Time Al - Identification and Enumeration of Fungal spores, plus total dander, fiber, and pollen count Hours 3//4/4 8-Std A- Identification and Enumeration of Fungal spores only Hours 3/64/4:Std D - Direct Identification of Fungi Hours 3034/4-Std 0 - Direct Identification of Mites, Insects, Pollen, etc Hours 3/8448-Std Cl - Culture Identification and Enumeration of Fungisinly 5-0 Days C - Culture Identification and Enumeration of Bacteria only -4 Days C3- Culture Identification and Enumeration of Fungi and Bacteria 5-0 Days -64- Culture Identification and Enumeration of Thermophilic Bacteria with C or C3 analysis -4 or 5-0 Days L -Culture Identification and Enumeration of Legianella S. 7-0 Days M - Dust Mite Allergen Test Hours 384/48-Std SanAlr Technologies Laboratom offers speclatlon by PCR. Please call for details and pricing. Sample 0 Sample Identification Sample Type Turn Total Anays l si Time Around Volume (L) Type(s) Start - Stop Time or Area in').8.406go otri SiDE Fie_ 47,zilloas-o W\ 6 Va_orA& it\-gcrrr.ec. i 80 0 Met uetk-vco tc. cknoa i 50,(; 707. / STO 7C L) Special Instructions [ Relinquished by Date r.4notatk (37-7 Li AC Time C( Rreived by \-li---- Date Jt.. $ ' Time L0.5 Unless scheduled, the turn around time for all samples received after pm Friday will begin at 8 am Monday morning. Weekend or Holiday work must be scheduled ahead of time and is charged 50% of analytical rate. 'Although we allow Direct Identification from a swab sample, best results are received from rape samples. Page ENCLOSURE I PAGE.5 OFS

8 ivestic Alf LkialityConsiltants 04 North Road (Rt. 7) Groton, CT I 30Vd -C 3E600NR 0 DAILY JOB LOG Client (aa.ko Site Location GENERAL OBSERVATIONS a N (0 Ct 6 MO un GKeurrict 0 CC S-r-itiJS.-A--lac) Ls Page ( of I Site Supervisor h\j LL co ICJ C-7 Containment Location LOLuet c_c-ver to N H as uu ri- cc& S i cv CS7-48U CH 6- AS/9- S cocia_s A..) a Tt jr/c LS OP igiattinict Date cri U4 ht-6 z> 7.0 Is SO -7E44" is 70r UYGIENIST'S HAME TINE CN SITE: 04 LA.-6C HYGIENIST'S SIGNATURE TIME OFF SITE: Telecommun ications Office: Nights & Weekends:

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