Curriculum Vitae. Personal information. Work experience. Education and training. First name / Surname. Laura Pancrazi. Date of birth 01/01/1984
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1 Curriculum Vitae Personal information First name / Surname Laura Pancrazi Date of birth 01/01/1984 Place of birth Sansepolcro (AR) Work experience Dates Occupation or position held Main activities and responsibilities Name and address of employer Type of business or sector Jan 2012-present Research Fellow I am involved in a project aimed at investigating the role of Nerve Growth Factor signalling in the pathogenesis of Alzheimer s disease and in the pathogenesis of congenital insensitivity to pain. The project implies the utilization of molecular biology techniques as cell culture, cloning, Western blot analysis., Piazza dei Cavalieri 7, Pisa (PI), Italy Research in Neurobiology Education and training Dates Jan present PhD I am involved in a project that implies the utilization of a mouse model for Rett Syndrome and both electrophysiological and molecular and cellular biology techniques. I am also involved in a project that investigates the role of Nerve Growth Factor (NGF) in the pathogenesis of Alzheimer s disease and the possibility to use NGF as a therapeutic tool. Supervisors: Prof. Antonino Cattaneo, Dr Mario Costa. ISCED6 and final Dates Oct Dec 2008 MSc in Biomolecular Sciences and Technology (Molecular and Cellular curriculum); final grade: 110/110 magna cum laude Molecular and Cellular Biology, Genetics and Genomics, Biochemistry, Statistics, Virology, Neurobiology. My MSc thesis work focused on molecular and developmental neurobiology. My MSc thesis title is A study for the generation of serotonergic neurons starting from an embryonic stem cell line knockin Tph2 EGFP(frt) and during my MSc thesis work I got a huge knowledge regarding the development of the nervous system and the generation of mouse models by gene targeting; I also acquired practical skills regarding cloning techniques, embryonic stem cell culture and Southern blot analysis to detect recombinant cell clones. Supervisor: Prof. Massimo Pasqualetti. ISCED5 Dates Oct Nov 2009 Second-level university diploma Neurobiology, Molecular Medicine, Molecular Virology. Title of the final dissertation: NGF mutants with altered intracellular signalling properties ; final mark: 70/70 cum laude. Supervisor: Prof Antonino Cattaneo.
2 and final and final Dates Oct July 2006 BSc in Molecular Biology; final grade 110/110 magna cum laude Cytology and Histology, Molecular and Cellular Biology, Chemistry and Biochemistry, Physiology, Microbiology, Genetics. I took my BSc degree with a thesis titled Knock down of Ku80 and relative consequences on homologous recombination. I have done the relative experimental work at the Molecular Biology Laboratory of under the supervision of Prof. Arturo Falaschi. Here I acquired a lot of knowledge and skills in Molecular Biology techniques, especially as regards RNAi, protein quantification and cell culture..external supervisor: Dott Giuseppe Rainaldi; internal supervisor: Prof. Stefano Landi. ISCED5 Dates Oct July 2006 First-level university diploma Mathematics, Physics, Biochemistry, Neurobiology, Molecular and Cellular Biology. Dates Sept July 2003 School-leaving certificate, final grade: 100/100 Mathematics, Physics, Biology, Chemistry, Latin, English Language and Literature, Italian Literature, History and Philosophy. Liceo Scientifico Città di Piero, Sansepolcro (Arezzo), Italy ISCED3 Boards Personal skills and competences Dates Dec 2010 Italian Qualifying Examination for Board Certified Biologist Mother tongue Italian Other languages English, French Self-assessment Understanding Speaking Writing European level (*) Listening Reading Spoken interaction Spoken production English C1 Proficient user C2 Proficient user B2 Independent user B2 Independent user C1 Proficient user French A2 Basic user A2 Basic user A2 Basic user A2 Basic user A2 Basic user
3 (*) Common European Framework of Reference for Languages Social skills and competences Organisational skills and competences Technical skills and competences Computer skills and competences Other skills and competences Driving licence Very good capacities to maintain good relationships and to efficiently interact with other people even in a stressful situation. Excellent capacities to coordinate a work together with other people. During my laboratory training I learned a lot of molecular and cellular biology techniques such as cell culture, mammalian cell transfection methods, Western and Southern blot, PCR, cloning techniques, mutagenesis, Fluorescence Recovery After Photobleaching, Raster Image Correlation Spectroscopy. I m also training in some electrophysiological techniques. I m able to use very efficiently Internet and online databases of biological interest. I also efficiently use Microsoft Office package. I usually use Windows operative system, but I can also use Linux and Mac. In 2004 I passed the European Computer Driving Licence START tests. I have a flair for Mathematics; in 2003 I ve been admitted to participate at the Italian national finals of Mathematics Olympiads. European driving licence, b category
4 Additional information Publications: Marchi M, Pancrazi L, Maffei M, Ratto GM, Costa M. ERK1 nucleo-cytoplasm shuttling rate depends on specific aminoacids in its N-terminus. Biochem Biophys Res Commun Jul 23;398(2): De Filippis R, Pancrazi L, Bjørgo K, Rosseto A, Kleefstra T, Grillo E, Panighini A, Cardarelli F, Meloni I, Ariani F, Mencarelli M, Hayek J, Renieri A, Costa M, Mari F. Expanding the phenotype associated with FOXG1 mutations and in vivo FoxG1 chromatin-binding dynamics. Clin Genet Nov 17. doi: /j x. [Epub ahead of print] Abstracts: Pancrazi L, Marchi M, Cattaneo A, Landsberger N, Kilstrup-Nielsen C, and Costa M. Spatio-temporal Dynamics and Localization of MeCP2 and FoxG1 in Living Cells. 2nd European Workibg group on Rett Syndrome, Stresa (VB), Italy, September 17 th - 19 th, 2009, p.29. Pancrazi L, Marchi M, Landsberger N, Costa M Spatio-temporal dynamics and localization of MeCP2, FOXG1and CDKL5 in living cells. I incontro scientifico dell Istituto di Neuroscienze, Cavalese (TN), Italy, January 13 th - 16 th, (poster) Pancrazi L, Panighini A, Marchi M, Hayek J, Renieri A, Maffei M, Costa M In vivo chromatin binding and intracellular localization of wild type and mutant Foxg1 proteins. 2nd European conference on Rett Syndrome, Edinburgh, Scotland (UK), October 7 th - 10 th, (poster) Mari F, Bjørgo K, Pancrazi L, Mencarelli MA, Ariani F, Melino I, Amenduni M, De Filippis R, Grillo E, Rosseto A, Pollazzon M, Hayek J, Costa M, Renieri A FOXG1 mutation leading to reduced chromatin affinity causes "Rett fruste" overlapping with EHMT1 phenotype. 2nd European conference on Rett Syndrome, Edinburgh, Scotland (UK), October 7 th - 10 th, (poster) Pancrazi L, Panighini A, Marchi M, Hayek J, Renieri A, Maffei M, Costa M In vivo chromatin binding and intracellular localization of wild type and mutant Foxg1 proteins. II incontro scientifico dell Istituto di Neuroscienze, Cavalese (TN), Italy, January 19 th - 22 nd, (poster) Pancrazi L., Della Sala G., Panighini A., Masala A., Cardarelli F., Maccari G., Colombaioni L. Costa M. Rett Syndrome and FOXG1 mitochondrial localization: is there a link?. III incontro scientifico dell Istituto di Neuroscienze, Brixen (BZ), Italy, February 19 th -March 23 rd, (poster). Pancrazi L., Della Sala G., Panighini A., Masala A., Cardarelli F., Maccari G., Colombaioni L. Costa M. Rett Syndrome and FOXG1 subcellular localization. Membrane Trafficking and Organelle Biogenesis Meeting, Bertinoro (FC), Italy, April 20 th -21 st, 2012 (oral presentation). Pancrazi L., Capsoni S., Costa M., Cattaneo A. Towards an NFG-based therapy for Hereditary Sensory and Autonomic Neuropathies IV and V. XVII Telethon Scientific Convention, Riva del Garda (TN), Italy; March 11 th -13 th, 2013 (poster). Selected congresses and seminars attendances: Gene Targeting into the 21st Century: Modelling Human Cancer In the mouse held by Professor Mario R. Capecchi, Toscan Cancer Institute, Florence, Italy, November 8 th, Organization and Plasticity of Visual Cortex,, Pisa, Italy, May 18 th - 19 th, The Brain in Health and Disease. Celebrating Rita Levi Montalcini s 100th birthday Rome; Italy, April 22 nd, I incontro scientifico dell Istituto di Neuroscienze Cavalese (TN) January 13 th - 16 th, nd European conference on Rett Syndrome, Edinburgh, Scotland (UK), October 7 th - 10 th, II incontro scientifico dell Istituto di Neuroscienze, Cavalese (TN,) January 19 th - 22 nd, 2011.
5 III incontro scientifico dell Istituto di Neuroscienze, Brixen (TN,) February 29 th March 3 rd, Membrane Trafficking and Organelle Biogenesis Meeting, Bertinoro (FC), Italy, April 20 th -21 st, 2012.XVII Telethon Scientific Convention, Riva del Garda (TN), Italy; March 11 th -13 th, 2013 Courses: June 3 rd - 9 th, 2011: partecipation at the Cold Spring Harbor Laboratory course Biology of memory July 21 st - August 3 rd, 2011: partecipation at the Cold Spring Harbor Laboratory course Biology of memory. Sicurezze: conoscere e condividere - Five hours module, seminar of information/training on chemical/carcinogenic-organic risk, Enclosures-ROA- Laboratory Waste Management, within the program of Information and Training of 'Research Area of CNR in Pisa I authorize the processing of my personal data.
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