Matrix metalloproteinase 1 and circulating tumor cells in early breast cancer.

Transcription

1 National Cancer Institute Slovakia Translational Research Unit Slovakia Matrix metalloproteinase 1 and circulating tumor cells in early breast cancer. Mego M 1, 2, Karaba M 2, Cierna Z 1, Janega P 1, Minarik G 1, Benca J 2, Sedl ckov T 1, Manasova D 1, Gronesova P 3, Pechan J 2,4, Reuben JM 5, Mardiak J 1, 2 1 Faculty of Medicine, Comenius University, Bratislava, Slovakia, 2 National Cancer Institute, Bratislava, Slovakia, 3 Cancer Research Institute, Bratislava, Slovakia, 4 Slovak Medical University, 5 University of Texas MD Anderson Cancer Center, Houston, Texas, USA

2 Matrix metalloproteinase 1 (MMP1)!!!!! zinc-dependent endopeptidase collagen-cleaving MMP cleave extracellular matrix components produced by tumor cells and tumor associated stroma high MMP1 expression in tumor is associated with: Ð! Ð! Ð! Ð! tumor evolution poor prognosis shortened survival induction of epithelial to mesenchymal transition

3 MMPs and EMT Radisky et al. J Mammary Gland Biol Neoplasia. 21, 15: 21-12

4 Mannello F. BMC Med. 211 Aug 11;9:95.

5 CTC heterogeneity Different methods detect different CTC subpopulations with different clinical and biological value All data regarding CTCs, should be interpreted within the context of the detection method used. Mego M, Mani SA, Cristofanilli M. Nat Rev Clin Oncol, 21

6 Factors affecting CTC count Mego M, Mani SA, Cristofanilli M. Nat Rev Clin Oncol, 21

7 Study hypothesis We hypothesize that MMP1 is involved in CTC release, and CTC are detected more often in breast cancer patients with high MMP1 expression in primary tumor.

8 Study aims! To assess association between CTC and MMP1 expression in breast cancer cells! To assess association between CTC and MMP1 expression in tumor stroma! To correlate MMP1 expression and patients` tumor characteristics

9 CTC detection

10 5 ml CTC detection RossetteSep CD45 depletion CD45 depleted cells RNA isolation, reverse transcription, quantitative RT-PCR for EMT related genes (TWIST,SNAIL,SLUG, ZEB2) and CK19) Quantitative RT-PCR dissociation curve Mego, et al. Int J Cancer, 212

11 MMP1 expression scoring! Immunohistochemical staining was quantified using a weighted histoscore.! The proportion of cells with nuclear staining was multiplied by the intensity of staining to provide a score of 3.! Score = ( x % not stained) + (1 x % weakly stained) + (2 x % moderately stained) + (3 x % strongly stained)

12 MMP1 expression scoring staining intensity staining intensity 1 staining intensity 2 staining intensity 3

13 Patients` characteristics All patients (N = 1) 1. % Invasive ductal carcinoma 86 T1 66 > T1 33 N 57 N ER/PR positive 87 HER2 positive 16 Triple negative 15 Grade 3 36

14 CTC detection Gene expression % samples KRT SNAIL1 SLUG 13 TWIST 2 ZEB1 Circulating tumor cells % patients All CTC 24 Epithelial CTC 12 EMT CTC 14 Both CTC 2

15 CTC and MMP1 expression in cancer cells M M P 1 a n d E p ith e lia l C T C MMP1 and EMT_CTC 1 5 p = p = MMP1 expression 1 5 C T C - C T C + CTC - CTC + E p ith e lia l C T C EMT_CTC M M P 1 a n d A n y C T C p =.37 C T C - C T C + A n y C T C

16 MMP1 expression in cancer cells and tumor characteristics M M P 1 e x p r e s s io n a n d tu m o r g r a d e M M P 1 a n d h o r m o n a l r e c e p to r s (H R ) 1 5 p =.4 2 p = G r a d e 1 a n d 2 G r a d e 3 H R + H R - M M P 1 a n d K i6 7 M M P 1 a n d tr ip le n e g a tiv e (T N ) b r e a s t c a n c e r p = p =.1 K I6 7 lo w K i6 7 h ig h N o n - T N T N

17 Multivariate analysis Independent variable Regression coefficient Standard error p-value EMT_ CTC (present vs. absent) Ki 67 (cut-off 14%) (low vs. high)

18 CTC and MMP1 expression in stromal cells 1 5 M M P 1 e x p r e s s io n a n d e p ith e lia l C T C p = M M P 1 e x p r e s s io n a n d E M T _ C T C p = C T C - C T C + C T C - C T C M M P 1 e x p r e s s io n a n d a n y C T C p = C T C - C T C +

19 MMP1 expression in stromal cells and tumor characteristics 1 5 M M P 1 e x p r e s s io n a n d tu m o r g r a d e M M P 1 e x p r e s s io n a n d tu m o r h is to lo g y p =.7 1 p = G r a d e 1 a n d 2 G r a d e 3 In v a s iv e d u c t a l c a r c in o m a O t h e r 1 5 M M P 1 e x p r e s s io n a n d K i6 7 p = K I6 7 lo w K I6 7 h ig h

20 Conclusions! High MMP1 expression in breast cancer cells, but not in cancer stroma was associated with CTC_EMT in peripheral blood! High MMP1 expression is associated with more aggressive biological features including increased proliferation, hormone receptor negativity and high grade.! Our data suggest a link between MMP1 and CTCs with EMT phenotype, and further support a role for MMPs and EMT in tumor dissemination.

21 Acknowledgements National Cancer Institute, Slovakia Department of Oncology Michal Mego Jozef Mardiak Jozef Sufliarsky Zlatica Péková Zuzana Hlavata Eva Oravcova Andrej Jurik Tomas Minarik Iveta Oravcova Michaela Kubi!ková Veronika "vábová Department of Surgery Marian Karaba Juraj Benca Juraj Pechan Translational Research Unit Denisa Ma#asová Daniela Svetlovska Department of Pathology Gabriela Sieberová Ján Macúch Michal Majer!ík Peter Jáni Comenius University, Faculty of Medicine Department of Pathology Zuzana $ierna Pavol Janega Ludovit Danihel Pavel Babal Institute of molecular biomedicine Gabriel Minárik Tatiana Sedla!ková Cancer Research Institute, Slovak Academy of Sciences Paulína Gronesová Ivana Fridrichová MD Anderson Cancer Center James M Reuben Jefferson Medical College, Philadelphia Massimo Cristofanilli

22 Thank you for your attention