PROGRAM. MONDAY, December 12 7:00 PM. Introductory Remarks. Andrew Hill, La Trobe Institute for Molecular Science, Melbourne, Australia

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1 PROGRAM MONDAY, December 12 7:00 PM Introductory Remarks SESSION 1 KEYNOTE LECTURES Andrew Hill, La Trobe Institute for Molecular Science, Melbourne, Australia Identification of a membrane transporter to uptake mature microrna Chen-Yu Zhang, Ke Zen, Qipeng Zhang, Qun Chen, Fan Zhang, Lei Dong [ ] Presenter affiliation: Najing University, Najing, China. 1 Exploiting exosomes biology in the diagnosis and treatment of pancreatic cancer Raghu Kalluri [ ] Presenter affiliation: MD Anderson Cancer Center, Houston, Texas. 2 TUESDAY, December 13 9:00 AM SESSION 2 LOADING AND FUNCTIONAL TRANSFER OF EV RNA Janusz Rak, McGill University, Montréal, Canada Mechanisms of mirna activity regulation by EV mediated export in mammalian cells Suvendra N. Bhattacharyya [ ] Presenter affiliation: CSIR-Indian Institute of Chemical Biology, Kolkata, India. 3 vii

2 Exosomes derived from mesenchymal phenotype lung cancer cells promote epithelial mesenchymal transition in epithelial cells through transfer of distinct small RNA cargo Yueting Tang, Yiyao Huang, Sihua Qin, Yong Xu, Taixue An, Lei Zheng [ ] Presenter affiliation: Nanfang Hospital, Southern Medical University, Guangzhou, China. 4 Coffee Break Exosomal transfer of MiR-151a enhances chemosensitivity to temozolomide in glioblastoma by targeting XRCC4 Ailiang Zeng, Wei Yan, Yongping You [10 +5 ] Presenter affiliation: The First Affiliated Hospital of Nanjing Medical University, Nanjing, China. 5 RNA profiles of three subtypes of extracellular vesicles released by human colorectal cancer LIM1863 cell lines Maoshan Chen, Rong Xu, Hong Ji, David Greening, Alin Rai, Richard Simpson [10 +5 ] Presenter affiliation: Department of Biochemistry and Genetics, Melbourne, Victoria, Australia. 6 Ubiquitination controls mirna-binding of HuR and augments extracellular export of mirna Kamalika Mukherjee, Suvendra N. Bhattacharyya [10 +5 ] Presenter affiliation: CSIR-Indian Institute of Chemical Biology, Kolkata, India. 7 Extracellular vesicle-mediated transfer of functional RNA between tissues Stefan Momma [ ] Presenter affiliation: University Hospital Frankfurt, Frankfurt am Main, Germany. 8 viii

3 TUESDAY, December 13 2:00 PM SESSION 3 POSTER SESSION Calcium-calpain dependent pathways regulate vesiculation in malignant breast cells Jack D. Taylor, Gregory R. Monteith, Mary Bebawy Presenter affiliation: The University of Technology Sydney, Sydney, Australia. 9 Long term stability of isolated human serum derived exosomes Candice de Boer, Neil H. Davies Presenter affiliation: University of Cape Town, Cape Town, South Africa. 10 Characterization of the DNA content of extracellular vesicle subsets Elisa Lázaro-Ibáñez, Rossella Crescitelli, Anaís García, Ganesh Shelke, Cecilia Lässer, Jan Lötvall Presenter affiliation: Krefting Research Centre, University of Gothenburg, Gothenburg, Sweden; Centre for Drug Research, Faculty of Pharmacy, Helsinki, Finland. 11 Exosomes secreted from human ips-derived mesenchymal stem cells prevent osteonecrosis of the femoral head by promoting angiogenesis Qing Li, Xiaolin Liu, Xin Niu, Bin Hu, Yang Wang Presenter affiliation: Institute of Microsurgery on Extremities, Shanghai, China. 12 BRAF V600 inhibition alters the microrna cargo in the vesicular secretome of malignant melanoma cells Taral R. Lunavat, Lesley Cheng, Berglind O. Einarsdottir, Roger O. Bagge, Robin A. Sharples, Cecilia Lässer, Yong S. Gho, Andrew F. Hill, Jonas A. Nilsson, Jan Lötvall Presenter affiliation: Krefting Research Centre, Gothenburg, Sweden. 13 Human ipsc-derived extracellular vesicles Characterization of their properties in vitro and in vivo Vasiliki Machairaki, Hao Bai, Yongxing Gao, Samarjit Das, Kenneth Kenneth Witwer, Linzhao Cheng Presenter affiliation: Johns Hopkins University School of Medicine, Baltimore, Maryland. 14 ix

4 Optimized EV isolation and EV-derived mirna analysis from urine and stool for colorectal cancer diagnosis Seung-ick Oh, Sookil Tae, Seokjin Ham, Hyunjoo Ko, Insoon Jang, Tae-Young Roh Presenter affiliation: Pohang University of Science and Technology, Pohang, South Korea. 15 Exosomes isolated from 3D-physiological oxygen cultured human Wharton s Jelly-derived mesenchymal stem cells (MSCs) induce cell death in the in-vitro cultured MCF 7 cells Balasubramanian Sundaram, Sanjay Kumar Presenter affiliation: Centre for Stem Cell Research, A unit of instem Bengaluru, Christian Medical College, Vellore, India. 16 Intercellular transmission of molecular chaperones via exosomes contributes to maintenance of organismal protein homeostasis Toshihide Takeuchi, Mari Suzuki, Nobuhiro Fujikake, Akiko H. Popiel, Hisae Kikuchi, Satoko Sakai, Shiroh Futaki, Keiji Wada, Yoshitaka Nagai Presenter affiliation: Osaka University, Osaka, Japan; National Center of Neurology and Psychiatry, Tokyo, Japan; Kyoto University, Kyoto, Japan. 17 Surface charge and pattern analysis as promising differentiating tools in scanning NTA Alireza Fazeli, Clemens Helmbrecht, Hanno Wachernig, Sascha Raschke Presenter affiliation: ; Particle Metrix, Meerbusch, Germany. 18 Exosomes secreted by human urine derived stem cells improves functional recovery after stroke in rats Guowei Zhang, Qingwei Zhu, Xiaozheng Ling, Xin Niu, Yang Wang, Zhifeng Deng Presenter affiliation: Institute of Microsurgery on Extremities, Shanghai, China. 19 Ionizing radiation affects mirna composition of exosomes Yi Wang, Sukhmani Billing, Michelle Budgen, Kei Cheng Mak, Farrah Flegal, Dmitry Klokov Presenter affiliation: Canadian Nuclear Laboratories, Chalk River, Canada. 20 x

5 Exosomes Characteristic and significance of cancer patient plasma Meng Xia, Ming-Bo Huang, Zhao Gao, Jing Leng Presenter affiliation: Guangxi University of Chinese Medicine, Nanning, China. 21 Curvature sensing MARCKS-ED peptides bind to membranes in a stereo-independent manner Lei Yan, Armando Jerome de Jesus, Ryo Tamura, Victoria Li, Kui Cheng, Hang Yin Presenter affiliation: Tsinghua University, Beijing, China. 22 Curcuma longa-derived nanoparticles reduce colitis and promote intestinal wound repair by inactivating NF-κB pathway Mingzhen Zhang, Emilie Viennois, Moon Kwon Han, Changlong Xu, Didier Merlin Presenter affiliation: Georgia State University, Atlanta, Georgia. 23 Impact of extracellular vesicles on host immune responses mediated by endosomal Toll-like receptors Ying Zhang, Hang Yin Presenter affiliation: Center of Basic Molecular Science, Beijing, China. 24 Thrombin-activated platelets-derived exosomes regulate ICAM-1 expression of endothelial cell via microrna-223 in thrombosisinflammation reaction Jiannan Li, Hongbing Yan, Zhou Zhou Presenter affiliation: Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China. 25 Exosomes released from human-induced pluripotent stem cellderived mesenchymal stem cells have superior effects than exosomes released from human synovial membrane derived mesenchymal stem cells for the treatment of osteoarthritis Yu Zhu, Yuchen Wang, Xin Niu, Yang Wang, Yunfeng Chen Presenter affiliation: Shanghai Jiao Tong University Affiliated Sixth People s Hospital, Shanghai, China. 26 TUESDAY, December 13 4:30 PM Chinese Tea and Beer Tasting xi

6 TUESDAY, December 13 7:00 PM SESSION 4 EVs AND THE CENTRAL NERVOUS SYSTEM Vasiliki Machairaki, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA The role of extracellular vesicles in neurodegenerative diseases Andrew F. Hill [ ] Presenter affiliation: La Trobe University, Bundoora, Australia. 27 Exosomes in neural cell communication 'Goodies' for neuronal fitness? Wen-Ping Kuo-Elsner, Carsten Frühbeis, Dominik Fröhlich, Eva-Maria Krämer-Albers [ ] Presenter affiliation: Johannes Gutenberg University (JGU) Mainz, Mainz, Germany. 28 Are exosomes responsible for the intercellular spread of Alzheimer's disease neuropathology? Laura J. Vella, Benjamin Scicluna, Lesley Cheng, Andrew F. Hill, Kevin Barnham [10 +5 ] Presenter affiliation: The Florey Institute of Neuroscience and Mental Health, Melbourne, Australia. 29 The extracellular vesicle environment in cell growth and HIV-1 production Kenneth W. Witwer [ ] Presenter affiliation: The Johns Hopkins University School of Medicine, Baltimore, Maryland. 30 xii

7 WEDNESDAY, December 14 9:00 AM SESSION 5 VESICLES: NOT JUST FOR MAMMALS Kenneth Witwer, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA EV-mediated transfer of RNA between helminths and their mammalian Hosts Amy H. Buck [ ] Presenter affiliation: University of Edinburgh, Edinburgh, United Kingdom. 31 Trypanosoma cruzi Trypomastigotes release EVs containing proinflammatory virulence factors that enhance host-cell invasion by Toll like receptor 2 Ana Claudia Torrecilhas, Rodrigo P. Soares [ ] Presenter affiliation: UNIFESP, São Paulo, Brazil. 32 Coffee Break Lipophosphoglycan (LPG), the major Leishmania surface glycoconjugate, is found in the extracellular vesicles (EVs) of Leishmania infantum and Leishmania braziliensis Rodrigo P. Soares, Armando Menezes-Neto, Blima Fux, Ana C. Torrecilhas [10 +5 ] Presenter affiliation: Centro de Pesquisas René Rachou/Fiocruz, Belo Horizonte, Brazil. 33 Gyongyi Szabo [ ] Presenter affiliation: University of Massachusetts Medical School, Worcester, Massachusetts. Extracellular membrane vesicles in hyperthermophilic archaea Sukhvinder Gill, Rie Matsumi, Evelyne Marguet, Jacques Oberto, Patrick Forterre [10 +5 ] Presenter affiliation: Institute for Integrative Biology of the Cell (I2BC), CEA, CNRS, Gif-sur-Yvette, France. 34 WEDNESDAY, December 14 2:00 PM Visit to Old Suzhou xiii

8 WEDNESDAY, December 14 7:00 PM SESSION 6 INTRA- AND EXTRACELLULAR VESICLES Takahiro Ochiya, National Cancer Center Research Institute, Tokyo, Japan Correlative light electron microscopy (CLEM) of endo-lysosomal compartments Judith Klumperman [ ] Presenter affiliation: University Medical Center Utrecht, Utrecht, Netherlands. 35 Types of secretory vesicles and their functions in axonal terminals Xu Zhang [ ] Presenter affiliation: Chinese Academy of Sciences, Shanghai, China. 36 Insights into endometrial-embryo interaction Defining the role of exosomes during implantation David W. Greening, Lois Salamonsen, Richard J. Simpson, Hong P. Nguyen, Kirstin Elgass [10 +5 ] Presenter affiliation: La Trobe University, Melbourne, Australia. 37 Free Open Bar Sponsored by BioBAY xiv

9 THURSDAY, December 15 9:00 AM SESSION 7 EVs IN CANCER PROGRESSION AND CELL-CELL COMMUNICATION I Lei Zheng, Nanfang Hospital, Southern Medical University, Guangzhou, China Extracellular vesicles as a novel diagnostic and therapeutic target for cancer Takahiro Ochiya [ ] Presenter affiliation: National Cancer Center, Tokyo, Japan. 38 Oncogenic driver mutations as regulators of cancer cell vesiculome Janusz Rak, Tae-Hoon Lee, Shilpa Chennakrishnaiah, Cristiana Spinelli, Esterina D'Asti, Dongsic Choi [ ] Presenter affiliation: McGill University, Montreal, Canada. 39 Coffee Break Migrasome and migracytosis Li Yu [ ] Presenter affiliation: Tsinghua University, Beijing, China. 40 Hypoxic exosome signature predicts disease progression in nonsmall cell lung cancer patients Andreas Möller [ ] Presenter affiliation: QIMR Berghofer Medical Research Institute, Brisbane, QLD, Australia. 41 Effects of lung cancer exosomes on mesenchymal stem cells and the underlying molecular mechanism Xiaoxia Li, Meiqian Xu, Shihua Wang, Qin Han, Robert Chunhua Zhao [10 +5 ] Presenter affiliation: Institute of Basic Medical Sciences&Chinese Academy of Medical Sciences, Beijing, China. 42 xv

10 THURSDAY, December 15 2:00 PM SESSION 8 EVs IN CANCER PROGRESSION AND CELL-CELL COMMUNICATION II Lei Zheng, Nanfang Hospital, Southern Medical University, Guangzhou, China Large oncosomes New frontiers for cell-to-cell communication in cancer Dolores Di Vizio [ ] Presenter affiliation: Cedars-Sinai, Los Angeles, California. 43 The complexities of multidrug resistance Mary Bebawy [ ] Presenter affiliation: The University of Technology Sydney, Sydney, Australia. 44 THURSDAY, December 15 3:30 PM SESSION 9 STEM CELLS AND EVs Mary Bebawy, University of Technology Sydney, Sydney, Australia MSC exosome preparation An assessment of homogeneity Sai Kiang Lim [ ] Presenter affiliation: Institute of Medical Biology, Singapore. 45 Exosomes secreted by human-induced pluripotent stem cellderived mesenchymal stem cells promote angiogenesis after stroke in rats Guowen Hu, Xiaozheng Ling, Qingwei Zhu, Xin Niu, Yang Wang, Zhifeng Deng [10 +5 ] Presenter affiliation: Department of Neurosurgery, Shanghai, China. 46 The multifaceted role of mesenchymal stem cell exosomes in cartilage regeneration Shipin Zhang, Ruenn Chai Lai, James Hoi Po Hui, Eng Hin Lee, Sai Kiang Lim, Wei Seong Toh [10 +5 ] Presenter affiliation: National University of Singapore, Singapore. 47 xvi

11 Effects of human umbilical cord blood-derived exosome on altered fetoplacental vascular function in gestational diabetes mellitus Julei Yao, Linyan Jia, Xinyao Zhou, Tao Duan, Kai Wang [10 +5 ] Presenter affiliation: Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, China. 48 Human ipsc-derived extracellular vesicles Characterization of their properties in vitro and in vivo Linzhao Cheng [10 +5 ] Presenter affiliation: Johns Hopkins University School of Medicine, Balitmore, Maryland. THURSDAY, December 15 6:00 PM COCKTAILS and BANQUET FRIDAY, December 16 9:00 AM SESSION 10 ADVANCES IN EV FUNCTIONALITY AND ENGINEERING Dolores Di Vizio, Cedars-Sinai, Los Angeles, California, USA Exosome and exosome-mimetics for theranostics Changjin Lee, Yong Song Gho [ ] Presenter affiliation: POSTECH, Pohang, South Korea. 49 Extracellular vesicle-sensing peptides derived from MARCKS block coagulation complex assembly Hang Yin [ ] Presenter affiliation: University of Colorado Boulder, Boulder, Colorado; Tsinghua University, Beijing, China. 50 Coffee Break From seeing to believing Visualization and tracking of extracellular vesicles Charles P. Lai [ ] Presenter affiliation: National Tsing Hua University, Hsinchu, Taiwan. 51 xvii

12 Definition and functionality of extracellular vesicle subtypes released simultaneously by dendritic cells Mercedes Tkach, Joanna Kowal, Clotlde Thery [ ] Presenter affiliation: Institut Curie, Paris, France; PSL Research University, Paris, France. 52 Development of High Sensitivity Flow Cytometry for the Quantitative and Multiparameter analysis of exosomes at the single-particle level Xiaomei Yan, Ye Tian, Manfei Gong, Guoqiang Su, Lin Ma, Wenqiang Zhang, Chaoxiang Chen, Shaobin Zhu [10 +5 ] Presenter affiliation: Xiamen University, Xiamen, China. 53 xviii