CANNABIS TESTING. LC/GC/SFC/SPE/QuEChERS. Analytical Options For Your Laboratory Needs.

Transcription

1 CANNABIS TESTING LC/GC/SFC/SPE/QuEChERS Analytical ptions For Your Laboratory Needs

2 If products in this guide do not provide at least an equivalent separation as compared to other products of the same phase and dimensions, send in your comparative data within 45 days and keep the product for FREE! 217, Inc. All rights reserved. 2

3 Your Testing Needs Get Started... pp. 4-5 Potency... pp. 6-7 Pesticides... pp. 8-9 Purifications/Extractions... pp Terpenes... p. 12 Residual Solvents... p. 13 Products...pp For complete details on applications visit: Search: 3

4 Sample Preparation The key to successful and effective analysis is sample preparation. Sample matrix effects can result in an array of interferences which can lead to poor chromatography as well as instrumentation drawbacks. Liquid Chromatography Improvements in particle morphologies and surface chemistries have increased analysis selectivity and productivity options. Simple Extraction Targeted Clean up Targeted Extraction Speed Efficiency Polar Selectivity Portability (HPLC UHPLC) Great Reproducibility Choose your options: pp Choose your options: pp Solid Phase Extraction Core-Shell Technology MEGA 4

5 Gas Chromatography Advancements in GC technology have provided more options to achieve the best separation and identification of a variety of pesticides, cannabinoids, terpenes and residual solvents for safer, higher quality cannabis products. SFC Columns High efficiency and great loading capacity for cannabis extractions purfications Rugged and Versatile Dependable Support and ptions Excellent Loadability Stable in: Normal Phase Reversed Phase Polar rganic SFC Conditions Choose your options: p.18 Choose your options: p.19 GC Columns Chiral LC Columns Your Tools for Success 5

6 Potency HPLC/UHPLC 12 Cannabinoids using Methanol and Kinetex 2.6 µm C18 LC Columns mau Core-Shell Technology CBDV CBDVA CBDA CBD CBG THC-V CBN CBG-A D9-THC D8-THC CBC THCA-A Column: Kinetex 2.6 µm C18 Part No.: B-4462-AN Dimensions: 5 x 2.1 mm Mobile Phase: A: Water with.1 % Formic acid** B: Methanol with.1 % Formic acid** ** Different acidic modifiers might affect the retention behavior of matrix peaks Gradient: Time (min) % B Flow Rate:.5 ml/min Back Pressure: 24 Bar Temperature: 5 C Detection: 23 nm min 12 Cannabinoids using Acetonitrile and Kinetex Core-Shell 2.6 µm Polar C18 LC Columns mau Core-Shell Technology CBDVA CBG-A CBN CBC App ID Elution rder MeH Elution rder AcCN CBDV 1 2 CBDVA 2 1 THC-V 3 7 CBD 4 6 CBG 5 5 CBDA 6 3 CBN 7 8 CBG-A 8 4 D9-THC 9 9 D8-THC 1 1 CBC THCA-A CBDV CBDA CBG CBD THC-V D9-THC D8-THC THCA-A App ID Column: Kinetex 2.6 µm Polar C18 Part No.: F-4759-E Dimensions: 15 x 4.6 mm Mobile Phase: A: Water with.1 % TFA** B: Acetonitrile with.1 % TFA** ** Different acidic modifiers might affect the retention behavior of matrix peaks Gradient: Time (min) % B Flow Rate: 1 ml/min Back Pressure: 15 Bar Temperature: 55 C Detection: 23 nm 2 4 min For more details visit TN

7 Potency UHPLC 1 Cannabinoids using Luna mega 1.6 µm Polar C18 UHPLC Columns mau Excellent resolution MEGA min 1 Cannabinoids using Kinetex Core-Shell 1.7 µm F5 UHPLC Columns mau Core-Shell Technology Faster analysis with similar peak order App ID 2358 Conditions for all columns: Columns: Luna mega 1.6 µm Polar C18 Kinetex 1.7 µm F5 Dimension: 1 x 2.1 mm Mobile Phase: A: 2 mm Ammonium Formate ph 3.2 B: Acetonitrile Gradient: Time (min) % B Flow Rate:.4 ml/min Temperature: 4 C Detection: 256 nm Sample: 1. CBVD 2. Cannabidiol 3. CBG 4. Cannabidolic Acid 5. CBG-A 6. Cannabinol 7. D-9 THC 8. D-8 THC 9. CBC 1. THCA-A App ID min 7

8 Pesticides GC-MS 112 Pesticides Screen Using Zebron ZB-MultiResidue-1 GC Columns ,95 96 GC Columns Column: Zebron ZB-MultiResidue -1 Dimensions: 3 meter x.25 mm x.25 µm Part No.: 7HG-G16-11 Injection: 26 C, 1 µl Recommended Liner: Zebron PLUS Single Taper Liner Part No.: AG2-A1-5 Carrier Gas: ml/min (constant flow) ven Program: 8 C for.5 min to 15 1 C/min to 24 4 C/min to C/min for 3 min Detector: 32 C; 45-4 amu Sample: Analytes are 1 ppm in Dichloromethane , , , App ID min 23 Pesticides from Leafy Produce Using Zebron ZB-5plus GC Columns , , , roq QuEChERS used for sample preparation 23 GC Columns min App ID Column: Zebron ZB-5plus with 5 µm Guardian Dimensions: 3 m x.25 mm x.25 µm Part No.: 7HG-G32-11-GGA Injection: 25 C, 2 μl Recommended Liner: Zebron PLUS Single Taper Liner Part No.: AG2-A1-5 Carrier Gas: 1 ml/min (constant flow) ven Program: 1 C to C/min to 28 1 C/min for 1 min then to C/min for 5 min Detector: 35 C Sample: 1. Dichlorvos 2. o-phenylphenol 3. Trifluarlin 4. a-hch-d6 5. Atrazine 6. Chlorothalonil 7. Chlorpyrifosmethyl 8. Carbaryl 9. d1-parathion 1. Chlorpyrifos 11. Cyprodnil 12. Tolyfluanid 13. Procymidone 14. o,p-ddd 15. Kresoxim-methyl 16. Ethion 17. Endosulfan Sulfate 18. Tebuconazole 19. Triphenyl Phosphate 2. Bifenthrin 21. L-Cyhalothrin 22. Permethrins 23. Azoxystrobin 8

9 Pesticides HPLC/UHPLC Intensity, cps Intensity, cps 64 Pesticides using Kinetex Core-Shell 2.6 µm Biphenyl LC Columns 5.8e6 5.5e6 5.e6 4.5e6 4.e6 3.5e6 3.e6 2.5e5 2.e5 1.5e5 1.e5 5.e5. 64 Pesticides using Kinetex Core-Shell 2.6 µm Polar C18 LC Columns 5.2e6 5.e6 4.e6 3.e6 2.e6 1.e min For more details visit TN-1224 Core-Shell Technology Core-Shell Technology min App ID App ID Column: Kinetex 2.6 µm Biphenyl Dimension: 5 x 4.6 mm Part No.: B-4622-E Mobile Phase: A: 1 mm Ammonium Acetate B: Methanol with.1 % Formic Acid Gradient: Time (min) 7 % B 1 Flow Rate:.7 ml/min Inj. Volume: 5 µl Temperature: 4 C Detection: MS/MS Detector: SCIEX API 4 Analyte: regon Pesticides List plus 5 For full list search APP ID Column: Kinetex 2.6 µm Polar C18 Dimension: 5 x 4.6 mm Part No.: B-4759-E Mobile Phase: A: 1 mm Ammonium acetate B: Methanol with.1 % formic acid Gradient: Time (min) 7 % B 1 Flow Rate:.7 ml/min Injection: 5 µl Temperature: 4 C Detection: MS/MS Instrument: SCIEX API 4 Analytes: For full list search APP ID

10 Purifications Reversed Phase/PREP Separation of 12 Cannabinoids using Luna mega Polar C18 LC Columns 1 Preparative options for scaling up MEGA App ID and min verlayed Chromatograms Column: Luna mega 3 µm Polar C18 Luna mega 5 µm Polar C18 Dimensions: 15 x 4.6 mm Part No.: F-476-E F-4754-E Mobile Phase: Water with.1 % Formic Acid / Acetonitrile with.1 % Formic Acid (25:75) Flow Rate: 1 ml/min Temperature: 22 C Detection: 23 nm Analytes: 1. Cannabidiol 2. Cannabidiolic Acid (CBDA) 3. Cannabinol (CBN) 4. D-9-Tetrahydrocannabinol (THC) 5. D-9-Tetrahydrocannabinolic Acid (THCA-A) 6. Cannbichromene (CBC) 7. Cannabigerol (CBG) 8. D-8-Tetrahydrocannabinol (THC) 9. Cannabidivarinic Acid (CBDVA) 1. Cannabigerolic Acid (CBGA) 11. Cannabidivarin (CBDV) 12. Tetrahydrocannabivarin (THCV) Luna 3 µm mega Polar C18 Luna 5 µm mega Polar C18 For more details visit or

11 Purifications Analytical/PREP 1 Cannabinoids using Lux 5 µm Cellulose-2 Chiral LC Columns Normalized Response 22 nm Chiral LC Columns min App ID Column: Lux 5 µm Cellulose-2 Dimensions: 25 x 4.6 mm Part No.: G-4457-E Mobile Phase: A: Carbon Dioxide B: Ethanol Gradient: Time (min) % B Injection: 2 µl Flow Rate: 6 ml/min Temperature: Ambient Backpressure: 12 Bar Detection: 22 nm Sample: 1. Cannabidivarin (CBDV) 2. Cannabidiol (CBD) 3. Cannabichromene (CBC) 4. Tetrahydrocannabivarin (THCV) 5. Tetrahydrocannabinol (THC) 6. Cannabinol (CBN) 7. Cannabigerol (CBG) 8. Cannabidiolic acid (CBD-A) 9. Tetrahydrocannabinolic Acid (THCA) 1. Cannabigerolic Acid (CBG-A) Note: 1 µg/ml mix of 1 cannabinoid standards obtained from Restek and Cayman Chemicals Instrument: JASC 4 analytical SFC (C2 pump PU-435, Co-solvent PU-438, PDA MD-41, BPR BP-434 AS AS-435) 13 Cannabinoids using Lux 3 µm Cellulose-2 Chiral LC Columns mau Enantiomers Separated Chiral LC Columns App ID Column: Lux 3 μm Cellulose-2 Dimensions: 15 x 3. mm Part No.: F-4456-Y Mobile Phase: A: Carbon Dioxide B: Methanol Gradient: Time (min) % B Flow Rate: 5 ml/min Detection: 22 nm Temperature: 4 C Sample: Cannabinoid mix of CBDV 2. CBN 3. D-8-THC 4. CBC (Enantiomer 1) 5. CBD 6. D-9-THC 7. CBC (Enantiomer 2) 8. THCV 9. CBG 1. CBDA 11. CBDVA 12. THCA 13. CBG-A min 11

12 Terpenes GC-FID and LC-MS 33 Primary and Secondary Terpenes by GC-FID using Zebron ZB-5PLUS GC Columns pa GC Columns min App ID Column: Zebron ZB-5PLUS Dimensions: 2 m x.18 mm x.36 μm Part No.: 7FD-G32-53 Guard: 5 m Z-Guard (7AD-G--GZ) Injection: Split 25 C, 1 μl Recommended Liner: Zebron PLUS Single Taper Z-Liner Liner Part No.: AG2-A13-1 Carrier Gas: 1.9 ml/min ven Program: 35 C to 15 1 C/min to C/min to C/min for 1.9 min Detector: 34 C Sample: Terpenes are 5-1 ppm in acetonitrile 1. α-pinene 2. Camphene 3. Myrcene 4. α-phellandrene 5. 3-Carene 6. α-terpinene 7. p-cymene 8. Limonene 9. cimene-1 1. cimene γ-terpinene 12. Sabinine hydrate 13. Terpineolene 14. Linalool 15. Fenchol 16. Isoborneol 17. Borneol 18. Menthol 19. α-terpineol 2. Citronellol 21. Pulegone 22. Geraniol 23. Geranyl acetate 24. Trans-Caryophyllene 25. α-humulene 26. Valencene 27. Nerolidol Nerolidol Caryophyllene oxide 3. Guaiol 31. α-bisabolol 32. Phytol Phytol-2 7 Primary Terpenes by LC-MS/MS Using Kinetex Core-Shell 2.6 µm Biphenyl LC Columns Intensity, cps 1.e7 9.e6 8.e6 7.e6 6.e6 5.e6 4.e6 3.e6 2.e6 1.e6.e Core-Shell Technology min App ID Column: Kinetex 2.6 μm Biphenyl Dimensions: 1 x 4.6 mm Part No.: D-4622-E Mobile Phase: A: 2 mm Ammonium acetate +.1 % Formic acid in Water B: 2 mm Ammonium acetate +.1 % Formic acid in Methanol Gradient: Time (min) B (%) Flow Rate:.55 ml/min Injection: 3 μl Temperature: Ambient Detection: MS/MS Instrument: SCIEX 35 Triple Quad Sample: 1. Linalool 2. Myrcene 3. Terpinolene 4. Limonene 5. a-pinene 6. Humulene 7. b-caryophyllene 12

13 Residual Solvents GC-FID Isobutane and Butane 1 ppm Standard 1 2 GC Columns App ID Residual Solvents including Butane and Isobutane 4 4 min Conditions for both: Column: Zebron ZB-624 Capillary GC Dimensions: 3 m x.32 mm x 1.8 µm Part No.: 7HM-G5-31 Injection: Split 25 C, 1 μl Carrier Gas: 2 ml/min (constant flow) ven Program: 35 C for 2 min to 25 C/min to 24 hold for 1. min Detector: 25 C Sample: 1. Isobutane 2. Butane 3. Acetone 4. IPA 5. ACN 6. DCM 7. EA 8. THF GC Columns 3 7 Rs = Rs = Rs = 2.89 App ID min 1 ppm Standard 13

14 Sample Preparation rdering Information QuEChERS, a quicker and easier sample preparation choice Step 1 Extraction Easily Weigh ut Samples No Mess Salt Packets riginal Method Packet 4. g Magnesium Sulfate 1. g Sodium Chloride Packet & Tube p/n: KS-891 Packet p/n: AH riginal Method Packet 4. g Magnesium Sulfate 1. g Sodium Chloride Packet & Tube p/n: KS-891 Packet p/n: AH riginal Method Packet 4. g Magnesium Sulfate 1. g Sodium Chloride Packet & Tube p/n: KS-891 Packet p/n: AH-942 Blend fruits or vegetables to be analyzed. Weigh blended sample. Add salts and acetonitrile. Shake tube for 1 minute. Centrifuge tube for 5 minutes. Step 2 riginal Method Packet 4. g Magnesium Sulfate 1. g Sodium Chloride Packet & Tube p/n: KS-891 Packet p/n: AH-942 Clean Up/dSPE Avoid Leaky Tubes! ur tubes are designed to seal completely riginal Method Packet 4. g Magnesium Sulfate 1. g Sodium Chloride Packet & Tube p/n: KS-891 Packet p/n: AH riginal Method Packet 4. g Magnesium Sulfate 1. g Sodium Chloride Packet & Tube p/n: KS-891 Packet p/n: AH Add supernatant from extraction procedure into a roq dspe tube. Shake dspe tube for 3 seconds. Centrifuge dspe tube for 5 minutes. roq Extraction Kits Extraction kits contain fifty easy-pour salt packets and fifty 5 ml standalone centrifuge tubes Description Unit Part No. AAC 27.1 Method Extraction Kits 6. g MgS 4, 1.5 g NaAc 5/pk KS-8911* EN Method Extraction Kits 4. g MgS 4, 1. g NaCl, 1. g SCTD,.5 g SCDS 5/pk KS-899* riginal Non-buffered Method Extraction Kits 4. g MgS 4, 1. g NaCl 5/pk KS g MgS 4, 1.5 g NaCl 5/pk KS-8912 *AAC and EN Extraction Kits also available in traditional non-collared 5 ml centrifuge tubes, Part No.: KS-8911-NC and KS-899-NC roq dspe Kits dspe kits contain pre-weighed sorbents/salts inside 2 ml or 15 ml centrifuge tubes Description Unit Part No. 2 ml dspe Kits 15 mg MgS 4, 25 mg PSA, 25 mg C18E 1/pk KS mg MgS 4, 25 mg PSA, 2.5 mg GCB 1/pk KS mg, MgS 4, 25 mg PSA, 7.5 mg GCB 1/pk KS mg MgS 4, 25 mg PSA 1/pk KS mg MgS 4, 5 mg PSA, 5 mg C18E, 5 mg GCB 1/pk KS mg MgS 4, 5 mg PSA, 5 mg C18E 1/pk KS mg MgS 4, 5 mg PSA, 5 mg GCB 1/pk KS mg MgS 4, 5 mg PSA 1/pk KS ml dspe Kits 9 mg MgS 4, 15 mg PSA, 15 mg C18E 5/pk KS mg MgS 4, 15 mg PSA, 15 mg GCB 5/pk KS mg MgS 4, 15 mg PSA, 45 mg GCB 5/pk KS mg MgS 4, 15 mg PSA 5/pk KS mg MgS 4, 4 mg PSA, 4 mg C18E, 4 mg GCB 5/pk KS mg MgS 4, 4 mg PSA, 4 mg C18E 5/pk KS mg MgS 4, 4 mg PSA, 4 mg GCB 5/pk KS mg MgS 4, 4 mg PSA 5/pk KS

15 Sample Preparation rdering Information Solid Phase Extraction (SPE) Targeted Extraction & Clean-up Polymeric SPE Strata-X polymer-based SPE sorbent designed to meet your separation needs. Acidic Neutral Basic Strong Acids (pk a < 2) Weak Acids (pk a 2 4) Neutral Compounds Weak Bases (pk a 8 1) Strong Bases (pk a > 1) Strata-X-AW Strata-X-A Strata-X Strata-X-C Strata-X-CW rdering Information Format Tube Strata-X Strata-X-A Strata-X-AW Strata-X-C Strata-X-CW Sorbent Mass Unit Part Number Part Number Part Number Part Number Part Number 1 mg 6 ml (3/box) 8B-S1-ECH 8B-S123-ECH 8B-S38-ECH 8B-S29-ECH 8B-S35-ECH 2 mg 3 ml (5/box) 8B-S1-FBJ 8B-S123-FBJ 8B-S38-FBJ 8B-S29-FBJ 8B-S35-FBJ 2 mg 6 ml (3/box) 8B-S1-FCH 8B-S123-FCH 8B-S38-FCH 8B-S29-FCH 8B-S35-FCH 5 mg 6 ml (3/box) 8B-S1-HCH 8B-S123-HCH 8B-S38-HCH 8B-S29-HCH 8B-S35-HCH Giga Tube 5 mg 12 ml (2/box) 8B-S1-HDG 8B-S123-HDG 8B-S38-HDG 8B-S29-HDG 1 g 12 ml (2/box) 8B-S1-JDG 8B-S123-JDG 8B-S38-JDG 8B-S29-JDG 8B-S35-JDG 1 g 2 ml (2/box) 8B-S1-JEG 8B-S123-JEG 8B-S38-JEG 8B-S29-JEG 8B-S35-JEG 2 g 2 ml (2/box) 8B-S1-KEG 8B-S123-KEG 8B-S29-KEG 8B-S35-KEG 5 g 6 ml (16/box) 8B-S1-LFF 8B-S123-LFF 8B-S38-LFF 8B-S29-LFF 8B-S35-LFF Watch How SPE Works 15

16 HPLC/UHPLC Chromatography rdering Information 1.7 μm Minibore Columns (mm) SecurityGuard ULTRA Cartridges Phases 3 x x x x 2.1 3/pk F5 B-4722-AN D-4722-AN F-4722-AN AJ-9322 Biphenyl A-4328-AN B-4628-AN D-4628-AN F-4628-AN AJ-929 XB-C18 A-4498-AN B-4498-AN D-4498-AN F-4498-AN AJ-8782 C18 A-4475-AN B-4475-AN D-4475-AN F-4475-AN AJ-8782 for 2.1 mm ID Core-Shell Technology 1.7 μm MidBore Columns (mm) SecurityGuard ULTRA Cartridges Phases 3 x 3. 5 x 3. 1 x 3. 3/pk XB-C18 A-4498-Y B-4498-Y D-4498-Y AJ-8775 C18 B-4475-Y D-4475-Y AJ-8775 HILIC B-4474-Y AJ-8779 for 3. mm ID 2.6 μm Microbore Columns (mm) Phases 5 x 1. 1 x x 1. XB-C18 B-4496-A D-4496-A F-4496-A 2.6 μm Minibore Columns (mm) SecurityGuard ULTRA Cartridges Phases 3 x x x x x 2.1 3/pk Polar C18 A-4759-AN B-4759-AN D-4759-AN F-4759-AN AJ-9532 F5 A-4723-AN B-4723-AN D-4723-AN F-4723-AN AJ-9322 Biphenyl A-4622-AN B-4622-AN D-4622-AN F-4622-AN AJ-929 XB-C18 A-4496-AN B-4496-AN C-4496-AN D-4496-AN F-4496-AN AJ-8782 C18 A-4462-AN B-4462-AN C-4462-AN D-4462-AN F-4462-AN AJ-8782 for 2.1 mm ID 2.6 μm MidBore Columns (mm) SecurityGuard ULTRA Cartridges Phases 3 x 3. 5 x x 3. 1 x x 3. 3/pk Polar C18 B-4759-Y D-4759-Y F-4759-Y AJ-9531 F5 B-4723-Y D-4723-Y F-4723-Y AJ-9321 Biphenyl B-4622-Y D-4622-Y F-4622-Y AJ-928 XB-C18 A-4496-Y B-4496-Y C-4496-Y D-4496-Y F-4496-Y AJ-8775 C18 A-4462-Y B-4462-Y C-4462-Y D-4462-Y F-4462-Y AJ-8775 for 3. mm ID 2.6 μm Analytical Columns (mm) SecurityGuard ULTRA Cartridges Phases 3 x x x x x 4.6 3/pk Polar C18 B-4759-E D-4759-E F-4759-E AJ-953 F5 B-4723-E D-4723-E F-4723-E AJ-932 Biphenyl B-4622-E D-4622-E F-4622-E AJ-927 XB-C18 B-4496-E C-4496-E D-4496-E F-4496-E AJ-8768 C18 A-4462-E B-4462-E C-4462-E D-4462-E F-4462-E AJ-8768 for 4.6 mm ID 5 μm Minibore Columns (mm) SecurityGuard ULTRA Cartridges Phases 3 x x x x 2.1 3/pk F5 A-4724-AN B-4724-AN D-4724-AN F-4724-AN AJ-9322 Biphenyl A-4627-AN B-4627-AN D-4627-AN AJ-929 XB-C18 A-465-AN B-465-AN D-465-AN AJ-8782 C18 A-461-AN B-461-AN D-461-AN F-461-AN AJ-8782 for 2.1 mm ID 5 μm MidBore Columns (mm) SecurityGuard ULTRA Cartridges Phases 5 x 3. 1 x x 3. 3/pk F5 B-4724-Y D-4724-Y F-4724-Y AJ-9321 Biphenyl B-4627-Y D-4627-Y F-4627-Y AJ-928 XB-C18 B-465-Y D-465-Y F-465-Y AJ-8775 C18 B-461-Y D-461-Y F-461-Y AJ-8775 for 3. mm ID 5 μm Analytical Columns (mm) SecurityGuard ULTRA Cartridges Phases 5 x x x x 4.6 3/pk F5 B-4724-E D-4724-E F-4724-E G-4724-E AJ-932 Biphenyl B-4627-E D-4627-E F-4627-E G-4627-E AJ-927 XB-C18 B-465-E D-465-E F-465-E G-465-E AJ-8768 C18 B-461-E D-461-E F-461-E G-461-E AJ-8768 for 4.6 mm ID SecurityGuard ULTRA Cartridges require holder, Part No.: AJ-9 * PREP SecurityGuard Cartridges require holder, Part No.: AJ-8223 ** PREP SecurityGuard Cartridges require holder, Part No.: AJ-8277 *** SemiPrep SecurityGuard Cartridges require holder, Part No.: AJ

17 HPLC/UHPLC Chromatography rdering Information 1.6 μm Microbore Columns (mm) Phases 5 x 1. 1 x x 1. Polar C18 B-4748-A D-4748-A F-4748-A MEGA 1.6 μm Minibore Columns (mm) SecurityGuard ULTRA Cartridges Phases 3 x x x x 2.1 3/pk Polar C18 A-4748-AN B-4748-AN D-4748-AN F-4748-AN AJ-955 C18 A-4742-AN B-4742-AN D-4742-AN F-4742-AN AJ-952 for 2.1 mm ID 3 μm Minibore Columns (mm) SecurityGuard Cartridges (mm) Phases 3 x x x x x 2.* Polar C18 A-476-AN B-476-AN D-476-AN F-476-AN AJ-76 for ID: mm 3 μm MidBore Columns (mm) SecurityGuard Cartridges (mm) Phases 5 x 3. 1 x x 3. 4 x 2.* Polar C18 B-476-Y D-476-Y F-476-Y AJ-76 for ID: mm 3 μm Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases 5 x x x x x 3.* Polar C18 B-476-E D-476-E F-476-E G-476-E AJ-761 for ID: mm 5 μm Minibore Columns (mm) SecurityGuard Cartridges (mm) Phases 3 x x x x x 2.* Polar C18 A-4754-AN B-4754-AN D-4754-AN F-4754-AN AJ-76 for ID: mm 5 μm MidBore Columns (mm) SecurityGuard Cartridges (mm) Phases 5 x 3. 1 x x 3. 4 x 2.* Polar C18 B-4754-Y D-4754-Y F-4754-Y AJ-76 for ID: mm 5 μm Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases 5 x x x x x 3.* Polar C18 B-4754-E D-4754-E F-4754-E G-4754-E AJ-761 for ID: mm 5 μm Axia Packed Preparative Columns (mm) SecurityGuard Cartridges (mm) Phases 15 x x x 3 15 x 21.2** 15 x 3. Polar C18 F-4754-P-AX G-4754-P-AX F-4754-U-AX AJ-763 AJ-764 for ID: 21.2 mm for ID: 3 mm 5 μm Axia Packed Preparative Columns (mm) SecurityGuard Cartridges (mm) Phases 25 x 3 25 x 5 15 x 21.2** 15 x 3. Polar C18 G-4754-U-AX G-4754-V-AX AJ-763 AJ-764 for ID: 21.2 mm for ID: 3 mm SecurityGuard ULTRA Cartridges require holder, Part No.: AJ-9 * SecurityGuard Analytical Cartridges require holder, Part No.: KJ-4282 ** PREP SecurityGuard Cartridges require holder, Part No.: AJ-8223 PREP SecurityGuard Cartridges require holder, Part No.: AJ-8277 Straggering UHPLC and HPLC Efficiency and Performance 17

18 GC Chromatography rder Information Zebron ZB-MultiResidue GC Columns ZB-MultiResidue-1 GC Columns Length (m) ID (mm) df (µm) Temp. Limits ( C) Part No to 32/34 7FD-G to 32/34 7HG-G to 32/34 7HM-G to 32/34 7HM-G to 32/34 7HK-G16-17 ZB-MultiResidue-2 GC Columns Length (m) ID (mm) df (µm) Temp. Limits ( C) Part No to 32/34 7HG-G to 32/34 7HM-G to 32/34 7HK-G17-17 Zebron ZB-624 GC Columns Zebron ZB-5plus GC Columns GC Columns Length (m) ID (mm) df (µm) Temp. Limits ( C) Part No to 36/37 7CD-G to 36/37 7EG-G to 36/37 7FD-G to 36/37 7HG-G to 36/37 7HG-G to 36/37 7HG-G to 36/37 7HM-G to 36/37 7HM-G to 36/37 7HM-G to 36/37 7KG-G32-11 Note: If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G5-27-B. Some exceptions may apply. Agilent 685 and some SRI and process GC systems use only 5 in. cages. Length (m) ID (mm) df (µm) Temp. Limits ( C) Part No to 26 7FD-G to 26 7HG-G to 26 7HM-G to 26 7HK-G to 26 7KG-G to 26 7KM-G to 26 7KK-G to 26 7LK-G to 26 7NK-G5-36 Note: If you need a 5 in. cage, simply add a (-B) after the part number, e.g., 7HG-G5-27-B. Some exceptions may apply. Agilent 685 and some SRI and process GC systems use only 5 in. cages. For more details visit 18

19 Supercritical Fluid Extractions rdering Information Lux High efficiency and loading capacity Stable in normal phase, polar organic, SFC, and reversed phase conditions 3 µm and 5 µm packed columns for separation and speed preferences Chiral LC Columns 5 µm Minibore and Analytical Columns (mm) SecurityGuard Cartridges (mm) Phases 5 x 2. 5 x x x x x 2.* 4 x 3.* i-cellulose-5 B-4756-E D-4756-E F-4756-E G-4756-E AJ-8631 AJ-8632 Cellulose-1 B-4459-B B-4459-E D-4459-E F-4459-E G-4459-E AJ-842 AJ-843 Cellulose-2 B-4457-B B-4457-E D-4457-E F-4457-E G-4457-E AJ-8398 AJ-8366 Cellulose-3 B-4493-B B-4493-E D-4493-E F-4493-E G-4493-E AJ-8621 AJ-8622 Cellulose-4 B-4491-B B-4491-E D-4491-E F-4491-E G-4491-E AJ-8626 AJ-8627 Amylose-1 B-4732-B B-4732-E D-4732-E F-4732-E G-4732-E AJ-9337 AJ-9336 Amylose-2 B-4472-B B-4472-E D-4472-E F-4472-E G-4472-E AJ-8471 AJ-847 for ID: mm mm *SecurityGuard Analytical Cartridges require holder, Part No.: KJ-4282 CH 3 CH 3 CI CI H 3 C H 3 C H 3 C CI NH NH NH NH Amylose Amylose Amylose Cellulose Lux Amylose-1 Lux Amylose-1 Lux Amylose-2 Lux i-cellulose-5 Amylose tris (3,5-dimethylphenylcarbamate) Amylose tris (3,5-dimethylphenylcarbamate) Amylose tris (5-chloro-2-methylphenylcarbamate) Cellulose tris (3,5-dichlorophenylcarbamate) H 3 C CH 3 CI CH 3 CH 3 H 3 C CI NH NH NH Cellulose--CNH Cellulose Cellulose Cellulose Lux Cellulose-1 Lux Cellulose-2 Lux Cellulose-3 Lux Cellulose-4 Cellulose tris (3,5-dimethylphenylcarbamate) Cellulose tris (3-chloro-4-methylphenylcarbamate) Cellulose tris(4-methylbenzoate) Cellulose tris (4-chloro-3-methylphenylcarbamate) 19

20 Accessories Selection rdering Information Phenex Syringe Filters Increase column life and save money! Ensure more accurate, consistent results mm diameter. 2. Hydrophobic membrane. Can be made hydrophilic by pre-wetting with IPA mm diameter mm diameter Phenex-RC (Regenerated Cellulose) Phenex-PTFE 2 (Polytetrafluoroethylene) Phenex-NY (Nylon) Phenex-GF/NY (Glass Fiber/Nylon) Phenex-PVDF (Polyvinylidene Fluoride) Phenex-GF/PVDF (Glass Fiber / Polyvinylidene Fluoride) 4 mm diameter for 2 ml sample volumes 15 mm diameter for 2 1 ml sample volumes mm diameter for 1 1 ml sample volumes Part No. Unit Part No. Unit Part No. Unit.2 µm.45 µm.2 µm.45 µm.2 µm.45 µm AF AF /pk AF AF /pk AF AF /pk AF AF /pk AF AF /pk AF AF /pk AF AF /pk AF AF /pk AF AF /pk AF AF /pk AF AF /pk AF AF /pk AF AF /pk AF AF /pk AF AF /pk AF AF /pk AF AF /pk AF AF /pk An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter AF-1A and a Nylon (NY) membrane. Excellent for filtration of particle-laden samples. Use AF-1B /pk less hand pressure to filter even the most difficult samples. utlet connection is luer lock. AF-1A AF-1B /pk AF AF /pk AF AF /pk AF AF /pk AF AF /pk An integrated syringe filter unit containing an inert borosilicate glass fiber prefilter and a PVDF membrane. The hydrophilic PVDF membrane provides high flow rates and throughput, low extractables and broad chemical compatibility. This membrane binds less protein than nylon or PTFE. AF6-6C6-12 AF6-6D6-12 1/pk AF6-6C6-52 AF6-6D6-52 5/pk Verex Assembled Vial Kits and Storage Vial Kits Clear or amber, with open top or closed caps PTFE (Teflon ) faced.125 in. silicone septa or PTFE with foam urethane backing Part No. Description Unit AR VA/ASE 4 ml Screw, Clear w/ Caps 24-4 white PTFE/Silicone, 1/pk open top AR A VA/ASE 4 ml Screw, Clear w/ Caps 24-4 white PTFE/Silicone, 1/pk (assembled), open top AR Storage, 2 ml Screw, Amber w/ Caps 24-4 white PTFE/Foam 1/pk Urethane liner, closed top AR Storage, 2 ml Screw, Clear w/ Caps 24-4 white PTFE/Foam Urethane liner, closed top 1/pk Verex Vials and Caps (Separate) Part No. Description Unit AR-35V-22 Vial, VA/ASE, 4 ml Screw, Clear, 24-4 Threads (No Cap) 2/pk AR B Cap (pre-assembled), Screw (24-414), w/ Bonded-in PTFE/Silicone septa, white 1/pk Headspace Screw-Top and Crimp-Top Seals / Closures Part No. Description Unit Crimp-Top AR Seal, 2 mm Diameter, PTFE/Silicone magnetic cap 1/pk AR Seal, 2 mm Diameter, PTFE/Silicone, silver 1/pk AR Seal, 2 mm Diameter, PTFE/Silicone Pressure Release, silver 1/pk Screw-Top AR-815M-13 Screw Cap, 18 mm, Magnetic, Silver, PTFE/Silicone septa (red/ 1/pk white) AR-81AM-13 Screw Cap, 18 mm, Magnetic, Silver, PTFE/Silicone septa (blue/ white) 1/pk Headspace Vials - Screw-Top and Crimp-Top Headspace Vials Part No. Description Unit Crimp-Top AR Headspace Vial, 23 x 75 mm, 2 ml Beveled Edge, Flat Bottom, 1/pk Clear, No Patch AR Headspace Vial, 23 x 75 mm, 2 ml Beveled Rim, Flat Bottom., 1/pk Clear, No Patch, Silanized AR Headspace Vial, 23 x 75 mm, 2 ml Square Rim, Flat Bottom, Clear, 1/pk No Patch AR Headspace Vial, 23 x 75 mm, 2 ml Beveled Edge, Round Bottom, 1/pk Clear, No Patch Screw-Top AR Headspace Vial, 23 x 75 mm, 2 ml 18 mm Screw, Round Bottom, 1/pk Clear, No Patch AR Headspace Vial, 23 x 75 mm, 2 ml 18 mm Screw, Round Bottom, Amber, No Patch 1/pk Part No. Description Unit Crimp-Top AR Headspace Vial, 23 x 75 mm, 2 ml Beveled Edge, Flat Bottom, 1/pk Clear, No Patch AR Headspace Vial, 23 x 75 mm, 2 ml Beveled Rim, Flat Bottom., 1/pk Clear, No Patch, Silanized AR Headspace Vial, 23 x 75 mm, 2 ml Square Rim, Flat Bottom, Clear, 1/pk No Patch AR Headspace Vial, 23 x 75 mm, 2 ml Beveled Edge, Round Bottom, 1/pk Clear, No Patch Screw-Top AR Headspace Vial, 23 x 75 mm, 2 ml 18 mm Screw, Round Bottom, 1/pk Clear, No Patch AR Headspace Vial, 23 x 75 mm, 2 ml 18 mm Screw, Round Bottom, Amber, No Patch 1/pk 2

21 Accessories Selection rdering Information Easy Seals Description Unit Part No. Easy Seal Inlet Base Seal, Gold Plated, for Agilent GCs 2/pk AG-8619 Easy Seal Inlet Base Seal, Gold Plated, for Agilent GCs 1/pk AG-862 Zebron PLUS GC Inlet Liners for Agilent Systems Description Applications Dimensions ID x L (mm) Unit Part No. Single Taper with Wool Semi-volatiles 4 x /pk 25/pk AG2-A11-5 AG2-A11-25 Single Taper Pesticides 4 x /pk 25/pk AG2-A1-5 AG2-A1-25 Single Taper Z-Liner Semi-volatiles Dirty samples 4 x /pk 25/pk AG2-A13-5 AG2-A13-25 Straight Z-Liner Straight Single Baffle Dirty samples Volatiles High initial oven temperatures Semi-volatiles Pesticides 4 x /pk 25/pk 1.8 x 71 5/pk 25/pk AG2-A3-5 AG2-A3-25 AG2-1F6-5 AG2-1F6-25 Zebron Z-Guard GC Column Protection Improve separation and peak shapes (especially early elutors) Extend column lifetime by preventing stationary phase damage ID (mm) Description Part No. Part No. 5 Meter 1 Meter.25 Guard Column 7AG-G--GZ 7CG-G--GZ Guard Column Kit 7AG-G--GZK 7CG-G--GZK.32 Guard Column 7AM-G--GZ 7CM-G--GZ Guard Column Kit 7AM-G--GZK 7CM-G--GZK.53 Guard Column 7AK-G--GZ 7CK-G--GZ Guard Column Kit 7AK-G--GZK 7CK-G--GZK Zebron Guardian Integrated Guard Columns Built-In Column Protection: No Leaks, No Worries! Eliminate the potential for leaks Extend column life Guardian System VS. Traditional Guard Column Potential for leaks Hassle to install Increased activity Continuous deactivation for better peak shape and inertness Seamless guard transition, no connection hassles and no potential for leaks 21

22 Accessories Selection rdering Information SecurityGuard Standard and PREP Column Protection* Effectively removes both contaminants and particulates to increase column lifetime Prevents micro-particulates and chemical contaminants from damaging your column and detector Choose your HPLC Column ID (mm) Material Description ph Stability Analytical SemiPrep Prep Cartridges for General Purpose 1/pk 1/pk 3/pk Ea Ea C18 (DS, ctadecyl) AJ-4286 AJ-4287 AJ-7221 AJ-7839 AJ-831 C8 (MS, ctyl) AJ-4289 AJ-429 AJ-7222 AJ-784 AJ-832 Silica AJ-4347 AJ-4348 AJ-7223 AJ-7229 AJ-8312 HILIC HILIC AJ-8328 AJ-8329 AJ-892 NH2 (Amino, Aminopropyl) AJ-431 AJ-432 AJ-7364 AJ-8162 AJ-839 CN (Cyano, Cyanopropyl) AJ-434 AJ-435 AJ-7313 AJ-822 AJ-8311 Phenyl (Phenylhexyl) AJ-435 AJ-4351 AJ-7314 AJ-7841 AJ-833 PFP(2) Pentafluorophenyl AJ-8326 AJ-8327 AJ-8376 AJ-8377 AJ-8378 Polar-RP (Ether-linked Phenyl) AJ-675 AJ-676 AJ-7276 AJ-7845 AJ-837 Fusion-RP (C18 Polar Embedded) AJ-7556 AJ-7557 AJ-7558 AJ-7844 AJ-836 Gemini NX-C18 (C18 TWIN-NX Technology) 1-12 AJ-8367 AJ-8368 AJ-8369 AJ-837 AJ-8371 Gemini C18 (C18 TWIN-NX Technology) 1-12 AJ-7596 AJ-7597 AJ-7598 AJ-7846 AJ-838 Gemini C6-Phenyl (C6-Phenyl TWIN Technology) 1-12 AJ-7914 AJ-7915 AJ-9156 AJ-9157 AJ-9158 Luna mega Polar C18 (Polar Function C18) AJ-76 AJ-761 AJ-9519 AJ-763 AJ-764 Guard Cartridge Holders (one-time purchase only) /kit /holder /kit /kit *For additional guard column phases see KJ-4282 AJ-9281 AJ-8223 AJ-8277 SecurityGuard ULTRA Column Protection ** Protects your columns and detectors from micro-particulates in the sample, solvent, or instrument Improve your UHPLC column lifetime Part No. Description Unit Cartridges for 4.6 mm ID UHPLC columns AJ-8768 C18 (DS, ctadecyl) with * ph stability 3/pk AJ-932 F5 (Pentafluorphenyl) with ph stability 3/pk AJ-877 C8 (MS, ctyl) with * ph stability 3/pk AJ-8774 Phenyl (Phenylhexyl) with * ph stability 3/pk AJ-8772 HILIC (HILIC) with ph stability 3/pk Holder AJ-9 SecurityGuard ULTRA Cartridge Holder Ea * ph stable under gradient conditions. ph stable under isocratic conditions **For additional guard column phases see HPLC / UHPLC Core-Shell Performance Enhancement Kits Increase method efficiency, resolution, and detection Improve results for sensitive and demanding applications Part No. Description Unit AQ-8892 Core-Shell Performance Enhancement Kit, Includes: PEEKsil Tubing, Fittings and Tool* Ea *Kit AQ-8892 includes the following components: Kit Quantity PEEKsil Tubing.1 mm ID x 1/16 in. D x 2 cm L, Red 2/pk PEEKsil Tubing.1 mm ID x 1/16 in. D x 1 cm L, Red Ea Sure-Lok High Pressure PEEK 1-Pc Nut, 1-32, for 1/16 in. Tubing 1/pk Sure-Lok Fitting Tightening Tool, Aluminum Ea 22

23 Complete Cannabis Testing Solutions HPLC-UHPLC-SFC-PREP-GC-SPE-QuEChERS Application Support & Resources Pesticide Residues Potency Terpenes Residual Solvents And More For more details visit 23

24 CANNABIS TESTING LC/GC/SFC/SPE/QuEChERS PRSRT STD U.S. PSTAGE PAID TRRANCE, CA PERMIT N. 185 Australia t: +61 () f: +61 () Austria t: +43 () f: +43 () Belgium t: +32 () (French) t: +32 () (Dutch) f: +31 () Canada t: +1 (8) f: +1 (31) China t: f: +86 () Denmark t: f: Finland t: +358 () f: France t: +33 () f: +33 () franceinfo@phenomenex.com Germany t: +49 () f: +49 () anfrage@phenomenex.com India t: +91 () f: +91 () indiainfo@phenomenex.com Ireland t: +353 () f: eireinfo@phenomenex.com Luxembourg t: +31 () f: +31 () nlinfo@phenomenex.com Mexico t: f: tecnicomx@phenomenex.com The Netherlands t: +31 () f: +31 () nlinfo@phenomenex.com New Zealand t: +64 () f: +64 () nzinfo@phenomenex.com Norway t: f: nordicinfo@phenomenex.com Puerto Rico t: +1 (8) 541-HPLC f: +1 (31) info@phenomenex.com Spain t: f: espinfo@phenomenex.com Sweden t: +46 () f: nordicinfo@phenomenex.com United Kingdom t: +44 () f: +44 () ukinfo@phenomenex.com USA t: +1 (31) f: +1 (31) info@phenomenex.com All other countries Corporate ffice USA t: +1 (31) f: +1 (31) info@phenomenex.com Italy t: f: italiainfo@phenomenex.com products are available worldwide. For the distributor in your country, contact USA, International Department at international@phenomenex.com BR568317_us Terms and Conditions Subject to Standard Terms and Conditions, which may be viewed at phenomenex.com/termsandconditions. Trademarks Kinetex, Luna, Lux, and Strata are registered trademarks of. Easy Seals, Giga, Guardian, MidBore, MultiResidue, Phenex, roq, SecurityGuard, Verex, 5plus, Z-Guard, Z-Liner, AXIA and Zebron and are trademarks of. Agilent is registered trademarks of Agilent Technologies, Inc. Triple Quad and API 4 are trademarks of AB SCIEX Pte. Ltd. AB SCIEX is being used under license. Restek is a registered trademark of Restek Corporation. Caymen Chemicals is a registered trademark of CAYMAN CHEMICAL CMPANY. JASC is a registered trademark of JASC Corporation. Strata-X is patented by. U.S. Patent No. 7,119,145. SecurityGuard is patented by. U.S. Patent No. 6,162,362 CAUTIN: this patent only applies to the analytical-sized guard cartridge holder, and does not apply to SemiPrep, PREP or ULTRA holders, or to any cartridges. 217, Inc. All rights reserved. 411 Madrid Avenue Torrance, CA USA