YMC CHIRAL columns. Polysaccharides NEA(R)(S) CD BR

Transcription

1 YMC CHIRAL columns Polysaccharides NEA(R)(S) CD BR

2 HPLC Columns for Optical Isomer Separation Chirality has become vitally important in the production of pharmaceuticals, agrochemicals, food and related products due to the different pharmacological or taste/odour effects which the different optical isomers can present. The pharmacological effects can range no activity through undesirable effects to having potentially life threatening adverse effects. This has led to the development of highly efficient CHIRAL stationary phases (CSP) for analytical and preparative scale separations. If the CSP is available in two enantiomeric configurations the elution order of enantiomeric pairs can be reversed. This is particularly useful when the two isomers are not present in equal quantities; a later eluting minor component can often be hidden by the tail of a major peak but on reversal of elution order can be totally resolved from the major component. 2 YMC Europe GmbH Phone: +49 (0) Fax: +49 (0) info@ymc.de Website:

3 Contents YMC CHIRAL Coated Polysaccharide Derivatives Series... page YMC CHIRAL Immobilised Polysaccharide Derivatives Series... page YMC CHIRAL NEA(R)(S)... page YMC CHIRAL CD BR... page Ordering Information... page * Application data by courtesy YMC Co., Ltd. 3

4 Coated Polysaccharides YMC CHIRAL Coated Polysaccharide Derivatives Series Polysaccharide chiral selector Wide application range With high durability, also for SFC/SMB Extremely attractive pricing for packed columns and preparative grade bulk media Introduction A new family of chiral products is announced by YMC, designed to be competitively positioned against Daicel and Phenomenex, specifically with regard to pricing. In addition and typical of YMC preparative grades are available in large quantities. Mobile phase and sample solvent The silica packing material is coated with the polysaccharide derivative. Therefore trace quantities of a solvent that might potentially dissolve the polysaccharide derivative (eg. THF, acetone, ethyl acetate, chloroform, dichloromethane, DMSO, DMF, etc.) should be eliminated. These solvents must be avoided for a mobile phase and the sample solvent. Effective for Preparative Separation of Enantiomers* CHIRALCEL OD-H YMC CHIRAL Cellulose-C Column: 5 µm, 250 x 4.6 mm ID Eluent: n-hexane/ethanol (90/10) Flow rate: 1.0 ml/min 4 YMC Europe GmbH Phone: +49 (0) Fax: +49 (0) info@ymc.de Website:

5 Coated Polysaccharides YMC CHIRAL Coated Polysaccharide Derivatives Series Specifications Item YMC CHIRAL Amylose-C YMC CHIRAL Cellulose-C Particle size 5, 10, 20 µm CHIRAL selector Type Separation mode Amylose tris (3,5-dimethylphenylcarbamate) (USP L51) Coating type Normal Phase Shipping solvent n-hexane/2-propanol (90/10) Temp. range 0-40 C Pressure limit Recommended flow rate 30 MPa (4350 psi) Cellulose tris (3,5-dimethylphenylcarbamate) (USP L40) 4.6 mm ID: ml/min (Max. flow rate: 3.0 ml/min) 10 mm ID: ml/min (Max. flow rate: 15 ml/min) Product Line-up Product name Particle size [µm] Chiral selector Type Competitive product YMC CHIRAL Amylose-C 5 10 YMC CHIRAL Cellulose-C 20 Amylose tris (3,5- dimethylphenylcarbamate Cellulose tris (3,5- dimethylphenylcarbamate Coating Coating CHIRALPAK AD, AD-H CHIRALCEL OD, OD-H CHIRALPAK and CHIRALCEL are registered trademarks of Daicel Corporation. * Application data by courtesy YMC Co., Ltd. 5

6 Coated Polysaccharides Stability using TFA Analysis of Ibuprofen* Column: YMC CHIRAL Amylose-C (5 µm) 250 x 4.6 mm ID Eluent: hexane / 2-propanol / TFA (99/1/0.1) Detection: UV at 220 nm Injection: 10 µl (1 mg/ml) Retention stability* 6 YMC Europe GmbH Phone: +49 (0) Fax: +49 (0) info@ymc.de Website:

7 Coated Polysaccharides Extended packing stability* Sequential gradient test x 20 runs Column performance test Sequential gradient test Column: 5 µm, 250 x 4.6 mm ID Eluent: A) n-hexane, B) ethanol 0-100% B (0-15 min) Flow rate: 3.0 ml/min Pressure: MPa/run Temperature: 37 C Column performance test Column: 5 µm, 250 x 4.6 mm ID Eluent: n-hexane/ethanol (90/10) Temperature: 37 C Detection: UV at 230 nm Sample: trans-stilbene oxide * Application data by courtesy YMC Co., Ltd. 7

8 Coated Polysaccharides Applications Benzoin* Benzoin* Column: YMC CHIRAL Amylose-C (5 µm) 250 x 4.6 mm ID Part No.: KAN99S WT Eluent: n-hexane / 2-propanol (90/10) Injection: 10 µl (0.1 mg/ml) Column: YMC CHIRAL Cellulose-C (5 µm) 250 x 4.6 mm ID Part No.: KCN99S WT Eluent: n-hexane / 2-propanol (90/10) Injection: 10 µl (0.1 mg/ml) N-CBZ-DL-Alanine* N-CBZ-DL-Alanine* Column: YMC CHIRAL Amylose-C (5 µm) 250 x 4.6 mm ID Part No.: KAN99S WT Eluent: n-hexane / 2-propanol / TFA (80/20/0.1) Injection: 10 µl (1 mg/ml) Column: YMC CHIRAL Cellulose-C (5 µm) 250 x 4.6 mm ID Part No.: KCN99S WT Eluent: n-hexane / 2-propanol / TFA (80/20/0.1) Injection: 10 µl (1 mg/ml) 8 YMC Europe GmbH Phone: +49 (0) Fax: +49 (0) info@ymc.de Website:

9 Coated Polysaccharides Duloxetine hydrochloride* Column: YMC CHIRAL Amylose-C (5 µm) 250 x 4.6 mm ID Part No.: KAN99S WT Eluent: n-hexane / ethanol / diethylamine (95/5/0.2) Temperature: 30 C Detection: UV at 230 nm Injection: 10 µl (0.1 mg/ml) Duloxetine hydrochloride* Column: YMC CHIRAL Cellulose-C (5 µm) 250 x 4.6 mm ID Part No.: KCN99S WT Eluent: n-hexane / ethanol / diethylamine (83/17/0.2) Temperature: 40 C Detection: UV at 230 nm Injection: 10 µl (The United States Pharmacopeia 37th; Limit of Duloxetine related compound A) * Application data by courtesy YMC Co., Ltd. 9

10 Coated Polysaccharides DL-Mandelic acid* DL-Mandelic acid* Column: YMC CHIRAL Amylose-C (5 µm) 250 x 4.6 mm ID Part No.: KAN99S WT Eluent: n-hexane / 2-propanol / TFA (80/20/0.1) Injection: 20 µl (1 mg/ml) Column: YMC CHIRAL Cellulose-C (5 µm) 250 x 4.6 mm ID Part No.: KCN99S WT Eluent: n-hexane / 2-propanol / TFA (80/20/0.1) Injection: 20 µl (1 mg/ml) trans-stilbene oxide* trans-stilbene oxide* Column: YMC CHIRAL Amylose-C (5 µm) 250 x 4.6 mm ID Part No.: KAN99S WT Eluent: n-hexane / 2-propanol (90/10) Detection: UV at 230 nm Injection: 5 µl (0.1 mg/ml) Column: YMC CHIRAL Cellulose-C (5 µm) 250 x 4.6 mm ID Part No.: KCN99S WT Eluent: n-hexane / 2-propanol (90/10) Detection: UV at 230 nm Injection: 5 µl (0.1 mg/ml) 10 YMC Europe GmbH Phone: +49 (0) Fax: +49 (0) info@ymc.de Website:

11 Coated Polysaccharides Atropine* Atropine* Column: YMC CHIRAL Amylose-C (5 µm) 250 x 4.6 mm ID Part No.: KAN99S WT Eluent: n-hexane / ethanol / ethanolamine (87/13/0.1) Detection: UV at 230 nm Injection: 2 µl (1 mg/ml) Column: YMC CHIRAL Cellulose-C (5 µm) 250 x 4.6 mm ID Part No.: KCN99S WT Eluent: n-hexane / ethanol / ethanolamine (90/10/0.1) Detection: UV at 230 nm Injection: 2 µl (1 mg/ml) Metoprolol* Metoprolol* Column: YMC CHIRAL Amylose-C (5 µm) 250 x 4.6 mm ID Part No.: KAN99S WT Eluent: n-hexane / ethanol / ethanolamine (80/20/0.1) Injection: 5 µl (1 mg/ml) Column: YMC CHIRAL Cellulose-C (5 µm) 250 x 4.6 mm ID Part No.: KCN99S WT Eluent: n-hexane / ethanol / ethanolamine (90/10/0.1) Injection: 5 µl (1 mg/ml) * Application data by courtesy YMC Co., Ltd. 11

12 Coated Polysaccharides Valsartan* (The United States Pharmacopeia) * Test solution was prepared from Valsartan supplied as a reagent for laboratory use. Column: YMC CHIRAL Cellulose-C (5 µm) 250 x 4.6 mm ID Part No.: KCN99S WT Eluent: n-hexane / 2-propanol / trifluoroacetic acid (85/15/0.1) Flow: 0.8 ml/min Detection: UV at 230 nm Injection: 10 µl (The United States Pharmacopeia 34th, Related compounds) Sertraline hydrochloride* (The European Pharmacopeia) * 1 Test solution and Reference solution were prepared from Sertraline hydrochloride supplied as a reagent for laboratory use. Column: YMC CHIRAL Amylose-C (5 µm) 250 x 4.6 mm ID Part No.: KAN99S WT Eluent: mixture* 2 / n-hexane (70/30) * 2 n-hexane / 2-propanol / diethylamine (75/25/1) Flow: 0.4 ml/min Detection: UV at 275 nm Injection: 20 µl (The draft for The European Pharmacopeia, Enantiomeric purity) 12 YMC Europe GmbH Phone: +49 (0) Fax: +49 (0) info@ymc.de Website:

13 Coated Polysaccharides Verapamil* Propanolol* Column: YMC CHIRAL Amylose-C (5 µm) 250 x 4.6 mm ID Part No.: KAN99S WT Eluent: n-hexane / 2-propanol / diethylamine (90/10/0.1) Injection: 10 µl (1 mg/ml) Column: YMC CHIRAL Cellulose-C (5 µm) 250 x 4.6 mm ID Part No.: KCN99S WT Eluent: n-hexane / 2-propanol / diethylamine (80/20/0.1) Detection: UV at 230 nm Injection: 10 µl (0.1 mg/ml) Column Care The recommended ph range for using YMC CHIRAL Polysaccharide derivatives columns is Remove acid and buffer salts before storage. Store the column in methanol/water = 50/50. If columns are affected by undesired contaminants or clogged inlet frits which cause back pressure increases, flush the column with THF in the opposite flow direction. For detailed information please refer to the Column Care and Use Instructions which are shipped with each analytical column. * Application data by courtesy YMC Co., Ltd. 13

14 Immobilised Polysaccharides YMC CHIRAL Immobilised Polysaccharide Derivatives Series Immobilised polysaccharide CHIRAL selector Applicable for normal and reversed phase modes Highly robust, also suitable for SFC/SMB Remarkably reduced background signal HPLC columns and preparative grade bulk media with particle sizes of 5, 10 and 20 µm available Extremely attractive pricing Introduction YMC CHIRAL polysaccharides derivatives are a series of CHIRAL separation columns / packing materials with high stereoselectivity. They are suitable for separations of wide range of chiral compounds, cis-trans isomers and geometric isomers. A range of particle sizes and column dimensions offer outstanding cost effectiveness from analytical to preparative separations. Immobilised Type Now, immobilised versions of YMC CHIRAL polysaccharides are announced. They can be used either in normal phase or in reversed phase modes. YMC CHIRAL immobilised polysaccharide derivatives are available in HPLC columns and in preparative grades, in large (multi kg) quantities. Excellent Peak Shape* Column: 5 µm, 250 x 4.6 mm ID Eluent: n-hexane / IPA / DEA (80/20/0.1) Temperature: 25 C Detection: 230 nm Injection: 10 µl (0.1 mg/ml) CHIRALPAK IB YMC CHIRAL Cellulose-SB YMC CHIRAL polysaccharides derivatives provide excellent peak shapes for ionic and metal coordinating compounds. Low Column Bleeding* Cellulose-SB CHIRALPAK IB Gradient test Column: 5 µm, 250 x 4.6 mm ID Eluent: A) n-hexane / B) ethanol 2-80% B (0-30 min), 80-2% B ( min), 2% B ( min) Temperature: 25 C Detection: UV at 230 nm 14 YMC Europe GmbH Phone: +49 (0) Fax: +49 (0) info@ymc.de Website:

15 Immobilised Polysaccharides Specifications YMC CHIRAL Immobilised Polysaccharide Derivatives Series Item YMC CHIRAL Amylose-SA YMC CHIRAL Cellulose-SB YMC CHIRAL Amylose-SE YMC CHIRAL Cellulose-SC Particle size 5, 10, 20 µm CHIRAL selector Type Separation mode Amylose tris (3,5-dimethylphenylcarbamate) Cellulose tris (3,5-dimethylphenylcarbamate) Immobilised type Amylose tris (3,5-dichlorophenylcarbamate) Normal Phase / Reversed Phase Cellulose tris (3,5-dichlorophenylcarbamate) Shipping solvent n-hexane / 2-propanol (90/10) Usable ph-range Pressure limit Recommended flow rate 30 MPa (4350 psi) 4.6 mm ID: ml/min (Max. flow rate: 3.0 ml/min) 10 mm ID: ml/min (Max. flow rate: 15 ml/min) Product Line-up Product name Particle size [µm] CHIRAL selector Type Competitive product YMC CHIRAL Amylose-SA Amylose tris (3,5-dimethylphenylcarbamate CHIRALPAK IA YMC CHIRAL Cellulose-SB YMC CHIRAL Amylose-SE Cellulose tris (3,5-dimethylphenylcarbamate Amylose tris (3,5-dichlorophenylcarbamate) Immobilised CHIRALPAK IB CHIRALPAK IE YMC CHIRAL Cellulose-SC Cellulose tris (3,5-dichlorophenylcarbamate) CHIRALPAK IC CHIRALPAK is a registered trademark of Daicel Corporation. * Application data by courtesy YMC Co., Ltd. 15

16 Immobilised Polysaccharides Wide Usable ph Range* Continuous flow of acid/alkaline solution x 1000 CV Column performance test Continuous flow of acid/alkaline solution Column: YMC CHIRAL Cellulose-SB 5 µm, 50 x 4.6 mm ID Eluent: buffer/methanol (90/10) Acidic condition Buffer: 0.1% H 3 PO 4 (ph 2) Temperature: 40 C Basic condition Buffer: 20 mm NH 4 HCO 3 -DEA (ph 9) Temperature: 25 C Column performance test Column: YMC CHIRAL Cellulose-SB 5 µm, 50 x 4.6 mm ID Eluent: acetonitrile/water (30/70) Temperature: 25 C Sample: Benzoin High Solvent Versatility* Column: 5 µm, 50 x 4.6 mm ID Eluent: n-hexane / 2-propanol (95/5) Sample: Benzoin 16 YMC Europe GmbH Phone: +49 (0) Fax: +49 (0) info@ymc.de Website:

17 Immobilised Polysaccharides Applications Donepezil* Donepezil* Column: YMC CHIRAL Cellulose-SB, 5 µm, 250 x 4.6 mm ID Part No.: KSB99S WT Eluent: 20 mm NH 4 HCO 3 -diethylamine (ph 9.0) / acetonitrile (40/60) Injection: 2 µl (200 µg/ml) Chloroquine* Column: YMC CHIRAL Amylose-SA, 5 µm, 250 x 4.6 mm ID Part No.: KSA99S WT Eluent: n-hexane / 2-propanol / diethylamine (80/20/0.1) Detection: UV at 230 nm Hydroxychloroquine* Column: YMC CHIRAL Amylose-SA, 5 µm, 250 x 4.6 mm ID Part No.: KSA99S WT Eluent: methyl tert-butyl ether / ethanol / diethylamine (95/5/0.1) Detection: UV at 344 nm Injection: 5 µl (100 µg/ml) Astaxanthin* Column: YMC CHIRAL Amylose-SA, 5 µm, 250 x 4.6 mm ID Part No.: KSA99S WT Eluent: methyl tert-butyl ether / ethanol / diethylamine (90/10/0.1) Detection: UV at 344 nm Injection: 5 µl (100 µg/ml) Warfarin* Column: YMC CHIRAL Cellulose-SB, 5 µm, 250 x 4.6 mm ID Part No.: KSB99S WT Eluent: n-hexane / THF (85/15) Flow rate: 0.5 ml/min Detection: UV at 476 nm Column: YMC CHIRAL Cellulose-SB, 5 µm, 250 x 4.6 mm ID Part No.: KSB99S WT Eluent: acetonitrile / 20 mm phosphoric acid (50/50) Injection: 2 µl (200 µg/ml) * Application data by courtesy YMC Co., Ltd. 17

18 NEA YMC CHIRAL NEA(R)(S) Normal and reversed phase modes Reversal of elution order Nonpolar to medium polar compounds Available in bulk quantities Specifications Item YMC CHIRAL NEA(R)(S) Particle size 5 µm Pore size 30 nm Surface area proprietary Carbon content proprietary Recommended ph range (reversed phase) Properties Although separation modes are chosen according to the purpose of separation, it is recommended to use one column dedicated for one separation mode in order to maximise the lifetime of the column. In normal phase mode YMC CHIRAL NEA allows the separation of a wide range of nonpolar to moderately polar compounds. The separation mechanisms involve a combination of: p-p interactions hydrogen bonding dipole interactions steric effects and for a successful separation at least three points of interaction between the CSP and the target compound must exist. Occasionally, for analytical separations, there may be a need to derivatise the sample with, for example p-donating groups such as dinitobenzoyl, dinitrophenylurea or dinitrophenylcarbamate groups. In some cases, the increase in detectability can offset the disadvantages of derivatisation. CHIRAL Separation Mechanism* A' A R B' B C' C A' A C B' B C' R 18 YMC Europe GmbH Phone: +49 (0) Fax: +49 (0) info@ymc.de Website:

19 NEA Applications using normal phase mode 1,1 -Bi-2-Naphthol* 2,2,2-Trifluoro-1-(9-anthryl) ethanol* Elution Order Reversal min YMC CHIRAL NEA(R) min YMC CHIRAL NEA(S) Column: YMC CHIRAL NEA (R) 250 x 4.6 mm ID Part No.: CR30S WT Eluent: hexane / dichloromethane / ethanol (70/30/2) Column: YMC CHIRAL NEA (R) and YMC CHIRAL NEA (S) 250 x 4.6 mm ID Part No.: CR30S WT and CS30S WT Eluent: hexane / dichloromethane / ethanol (70/30/1) Flow rate: 0.5 ml/min Hexobarbital* 1-Phenylethanol* carbamate formation min Column: YMC CHIRAL NEA (R) 250 x 4.6 mm ID Part No.: CR30S WT Eluent: hexane / CH 2 Cl 2 / ethanol (90/10/2) Temperature: ambient Detection: UV at 220 nm Column: YMC CHIRAL NEA (R) 250 x 4.6 mm ID Part No.: CR30S WT Eluent: hexane / CH 2 Cl 2 / ethanol (90/10/5) Temperature: 35 C * Application data by courtesy YMC Co., Ltd. 19

20 NEA Applications using reversed phase mode 1,1 -Bi-2-Naphthol* Propranolol HCl* Column: YMC CHIRAL NEA (R) 250 x 4.6 mm ID Part No.: NR30S WT Eluent: acetonitrile / water (50/50) Pressure: 80 bar Detection: UV at 235 nm Injection: 1.0 µl (2.8 mg/ml) Temperature: ambient a) Column: YMC CHIRAL NEA (R) 250 x 4.6 mm ID Part No.: NR30S WT Eluent: acetonitrile / 0.5M NaClO 4 (40/60) Temperature: ambient Time: 100 hours b) Eluent: acetonitrile / 0.5M NaClO 4 (40/60) Temperature: ambient Hexobarbital* CBZ-Phenylalanine (Z-Phe-OH)* Column: YMC CHIRAL NEA (R) 250 x 4.6 mm ID Part No.: NR30S WT Eluent: 0.5M NaClO 4 -HClO 4 (ph 2.0) / acetonitrile (70/30) Injection: 10 µl (1.5 mg/ml) Temperature: ambient Column: YMC CHIRAL NEA (R) 250 x 4.6 mm ID Part No.: NR30S WT Eluent: acetonitrile / water (30/70) Flow: 0.7 ml/min Detection: UV at 210 nm Injection: 1.0 µl (1.2 mg/ml) Temperature: ambient 20 YMC Europe GmbH Phone: +49 (0) Fax: +49 (0) info@ymc.de Website:

21 NEA Applications using reversed phase mode 2,2,2-Trifluoro-1-(9-anthryl)-ethanol* Ketamin HCl* Column: YMC CHIRAL NEA (R) 250 x 4.6 mm ID Part No.: NR30S WT Eluent: acetonitrile / water (40/60) Injection: 1.0 µl (0.14 mg/ml) Temperature: ambient Column: YMC CHIRAL NEA (R) 250 x 4.6 mm ID Part No.: NR30S WT Eluent: acetonitrile / 0.5M NaClO 4 (40/60) Detection: UV at 268 nm Injection: 10 µl (1.4 mg/ml) Temperature: ambient Column Care The recommended ph range for using YMC CHIRAL NEA(R)(S) columns is Remove acid and buffer salts before storage. Store the column in methanol/water = 50/50. If columns are affected by undesired contaminants or clogged inlet frits which cause back pressure increases, flush the column with THF in the opposite flow direction. For detailed information please refer to the Column Care and Use Instructions which are shipped with each analytical column. * Application data by courtesy YMC Co., Ltd. 21

22 CD BR YMC CHIRAL CD BR Reversed phase Polar pharmaceuticals Positional isomers Water-soluble compounds Specifications Item YMC CHIRAL CD BR Particle size 5 µm Pore size 12 nm Surface area proprietary Carbon content proprietary Recommended ph range Properties YMC CHIRAL CD BR columns offer an alternative approach to enantioseparation. Covalent bonding of a bromide derivative of a cyclodextrin to YMC silica provides a novel CSP. The bromide derivative, in which the primary hydroxyl groups at carbon 6 are substituted for bromine, provides a different CHIRAL selectivity to the normal cyclodextrins. These cyclodextrin bromide derivatives are used in reversed phase mode to separate a wide range of polar, water-soluble compounds. In addition they will separate, under similar conditions, positional isomers of substituted aromatic compounds. HO HO HO HO HO HO HO HO HO HO HO Br Br Br Br NH Br S IL I C A HO HO Br HO hydrophobic cavity hydrophilic rim -CD BR 22 YMC Europe GmbH Phone: +49 (0) Fax: +49 (0) info@ymc.de Website:

23 CD BR Hexobarbital* Ketoprofen* Column: YMC CHIRAL -CD BR 250 x 4.6 mm ID Part No.: DB12S WT Eluent: 0.1M acetic acid-triethylamine in water (ph 5.6) / methanol (30/70) Temperature: 30 C Injection: 5 µl (1 mg/ml) Column: YMC CHIRAL -CD BR 250 x 4.6 mm ID Part No.: DB12S WT Eluent: 0.1M acetic acid-triethylamine in water (ph 5.6) / methanol (30/70) Injection: 10 µl (1 mg/ml) Fluorbiprofen* Column: YMC CHIRAL -CD BR 250 x 4.6 mm ID Part No.: DB12S WT Eluent: 0.1M acetic acid-triethylamine in water (ph 4.0) / acetonitrile (10/90) Injection: 2 µl (1 mg/ml) Column: YMC CHIRAL -CD BR 250 x 4.6 mm ID Part No.: DG12S WT Eluent: 0.1M acetic acid-triethylamine in water (ph 4.0) / methanol (30/70) Injection: 2 µl (1 mg/ml) * Application data by courtesy YMC Co., Ltd. 23

24 CD BR 1-(1-naphthyl)-ethylalcohol* Phenylethylalcohol* Column: YMC CHIRAL -CD BR 250 x 4.6 mm ID Part No.: DG12S WT Eluent: 0.1M AcOH aq -TEA (Triethylamine) aq (ph5.6) / methanol (90/10) Temperature: 30 C Injection: 5 µl (1 mg/ml) Column: YMC CHIRAL -CD BR 250 x 4.6 mm ID Part No.: DG12S WT Eluent: 0.1M AcOH aq -TEA (Triethylamine) aq (ph4.0) / methanol (90/10) Temperature: 30 C Injection: 5 µl (10 mg/ml) Cresols* Column: YMC CHIRAL -CD BR 250 x 4.6 mm ID Part No.: DB12S WT Eluent: methanol / water (20/80) Temperature: ambient Column: YMC Pack ODS-AM 250 x 4.6 mm ID Part No.: AM12S WT Eluent: methanol / water (40/60) Temperature: ambient 24 YMC Europe GmbH Phone: +49 (0) Fax: +49 (0) info@ymc.de Website:

25 CD BR Estradiols* Propranolol HCl* Column: YMC CHIRAL -CD BR 250 x 4.6 mm ID Part No.: DG12S WT Eluent: methanol / water (50/50) Temperature: 30 C Detection: UV at 230 nm Nitrophenols* Column: YMC CHIRAL -CD BR 250 x 4.6 mm ID Part No.: DG12S WT Eluent: acetonitrile / methanol / acetic acid / triethylamine (99/1/0.3/0.25) Injection: 5 µl (1 mg/ml) Column: YMC CHIRAL -CD BR 250 x 4.6 mm ID Part No.: DA12S WT Eluent: 0.1M CH 3 COOH-CH 3 COONa (ph4.0) / methanol (90/10) Column Care The recommended ph range for using YMC CHIRAL CD BR columns is Remove acid and buffer salts before storage. Store the column in methanol/water = 50/50. If columns are affected by undesired contaminants or clogged inlet frits which cause back pressure increases, flush the column with THF in the opposite flow direction. For detailed information please refer to the Column Care and Use Instructions which are shipped with each analytical column. * Application data by courtesy YMC Co., Ltd. 25

26 Ordering Information YMC CHIRAL Amylose-C Phase dimension 5 µm Column ID [mm] Column length [mm] Guard cartridges* with 10/23 mm length 2 KAN99S WT KAN99S WT KAN99S CC (10 mm length, pack of 2) 4.6 KAN99S WT KAN99S WT KAN99S05-G304CC (23 mm length, pack of 3) 10 KAN99S WT KAN99S WTG (30 mm length, 1 unit) 20 KAN99S WX KAN99S CC (10 mm length, pack of 2) 30 KAN99S WX KAN99S CC (10 mm length, pack of 2) YMC CHIRAL Cellulose-C Phase dimension 5 µm Column ID [mm] Column length [mm] Guard cartridges* with 10/23 mm length 2 KCN99S WT KCN99S WT KCN99S CC (10 mm length, pack of 2) 4.6 KCN99S WT KCN99S WT KCN99S05-G304CC (23 mm length, pack of 3) 10 KCN99S WT KCN99S WTG (30 mm length, 1 unit) 20 KCN99S WX KCN99S CC (10 mm length, pack of 2) 30 KCN99S WX KCN99S CC (10 mm length, pack of 2) YMC CHIRAL Amylose-SA Phase dimension 5 µm Column ID [mm] Column length [mm] Guard cartridges* with 10/23 mm length 2 KSA99S WT KSA99S WT KSA99S CC (10 mm length, pack of 2) 4.6 KSA99S WT KSA99S WT KSA99S05-G304CC (23 mm length, pack of 3) 10 KSA99S WT KSA99S WTG (30 mm length, 1 unit) 20 KSA99S WX KSA99S CC (10 mm length, pack of 2) 30 KSA99S WX KSA99S CC (10 mm length, pack of 2) YMC CHIRAL Cellulose-SB Phase dimension 5 µm Column ID [mm] Column length [mm] Guard cartridges* with 10/23 mm length 2 KSB99S WT KSB99S WT KSB99S CC (10 mm length, pack of 2) 4.6 KSB99S WT KSB99S WT KSB99S05-G304CC (23 mm length, pack of 3) 10 KSB99S WT KSB99S WTG (30 mm length, 1 unit) 20 KSB99S WX KSB99S CC (10 mm length, pack of 2) 30 KSB99S WX KSB99S CC (10 mm length, pack of 2) *Guard cartridge holder required, part no. XPCHSMW (2 mm ID) XPCHW (4 mm ID) XPCHSPW2 (20 mm ID) XPCHSPW3 (30 mm ID) no holder required for 10 mm ID 26 YMC Europe GmbH Phone: +49 (0) Fax: +49 (0) info@ymc.de Website:

27 Ordering Information Normal Phase: YMC CHIRAL NEA(R)(S) Phase dimension 30 nm 5 µm NEA(R) 30 nm 5 µm NEA(S) Column ID [mm] Column length [mm] Guard cartridges* with 10 mm length [pack of 5] 4.6 CR30S WT CR30S WT CR30S WT CR30S WT CR30S GC 4.6 CS30S WT CS30S WT CS30S WT CS30S WT CS30S GC *Guard cartridge holder required, part no. XPGCH-Q1 Reversed Phase: YMC CHIRAL NEA(R)(S) Phase dimension 30 nm 5 µm NEA(R) 30 nm 5 µm NEA(S) Column ID [mm] Column length [mm] Guard cartridges* with 10 mm length [pack of 5] 4.6 NR30S WT NR30S WT NR30S WT NR30S WT NR30S GC 4.6 NS30S WT NS30S WT NS30S WT NS30S WT NS30S GC *Guard cartridge holder required, part no. XPGCH-Q1 a-cd BR: YMC CHIRAL CD BR Phase dimension 12 nm 5 µm Column ID [mm] Column length [mm] Guard cartridges* with 10 mm length [pack of 5] 4.6 DA12S WT DA12S WT DA12S WT DA12S GC *Guard cartridge holder required, part no. XPGCH-Q1 b-cd BR: YMC CHIRAL CD BR Phase dimension 12 nm 5 µm Column ID [mm] Column length [mm] Guard cartridges* with 10 mm length [pack of 5] 4.6 DB12S WT DB12S WT DB12S WT DB12S GC *Guard cartridge holder required, part no. XPGCH-Q1 g-cd BR: YMC CHIRAL CD BR Phase dimension 12 nm 5 µm Column ID [mm] Column length [mm] Guard cartridges* with 10 mm length [pack of 5] 4.6 DG12S WT DG12S WT DG12S WT DG12S GC *Guard cartridge holder required, part no. XPGCH-Q1 * Application data by courtesy YMC Co., Ltd. 27

28 Your local distributor: Co., Ltd. YMC Karasuma-Gojo Bld. 284 Daigo-cho, Karasuma Nisiiru Gojo-dori Shimogyo-ku, Kyoto Japan TEL. +81(0) , FAX +81(0) Europe GmbH Schöttmannshof 19 D Dinslaken Germany TEL. +49(0)2064/427-0, FAX +49(0)2064/ America, Inc. 941 Marcon Boulevard Suite 201 Allentown, PA18109 USA TEL , FAX Taiwan Co., Ltd. 3F, No. 1353, Zhongzheng Rd., Taoyuan City, Taoyuan Country 330, Taiwan (R.O.C.) TEL , FAX Korea Co., Ltd. #208, Owners Tower, 16-5, Sunae-dong, Bundang-gu, Seongnam-si, Gyeonggi-do, Korea TEL , FAX India Pvt. Ltd. A-154, Eros Boulevard Hilton Hotel Complex Plot No.13-B, District Centre Mayur Vihar Phase-I New Delhi India TEL ; , FAX Co., Ltd. Shanghai Rep. Office Far East International Plaza A2404 No. 319 Xianxia Road, Shanghai P.R. China TEL , FAX Singapore Tradelinks Pte. Ltd. 6 Indus Road, #13-09 Emerald Park, Tower 1, Singapore TEL CHIRAL Columns 10/14_EN