Orbitrap technology update

Transcription

1 Orbitrap technology update Users Meeting Somerset, Oct 12, 211 Dr. Thomas Moehring

2 Outline Users Meeting 21 Technology Update Orbitrap Elite - Q Exactive Summary 2

3 Your Goal: The ideal mass analyzer All-in-one device suitable for every purpose 3

4 Users Meeting 21: What to expect from Orbitrap mass spectrometry? Faster and more powerful Orbitraps High-field Orbitraps (dimensions, voltage) Higher-performance (resolving power, mass accuracy) Orbitraps Higher space charge capacity C-trap Intelligent is better than fast : multiple fills allow to improve quality of analysis Stepped collision energy in 1 scan Multiple SIM windows in 1 scan Multiple MS/MS in 1 scan... 4

5 Technology update Orbitrap Elite

6 Technology Update: Compact High-field Orbitrap analyzer 1.5 x 1.2 x Standard Orbitrap High-field Orbitrap Smaller size- 1.8x frequency at the same voltage 1.8 x higher resolution over standard Orbitrap New lenses for focusing ions onto Orbitrap entrance Change of Orbitrap geometry for the first time since the introduction of Orbitrap technology in 25!!! Prof. Neil Kelleher, Kelleher Lab,Northwestern University: This is not an incremental improvement! 6

7 Original Orbitrap and High Field Orbitrap Real Size Cutaways Orbitrap Elite LTQ Orbitrap Classic/XL/Discovery/Velos(Pro) (Q)Exactive 7

8 Advanced Signal Processing A) Initial phase degrees Magnitude spectra B) Initial phase -45 degrees Fourier transformations provide a complex value consisting of Absorption spectrum Dispersion spectrum Mixedmode spectrum Mixed-mode spectrum Figure courtesy of R. Malek magnitude and phase component On all our previous FT mass spectrometers magnitude mass spectra were generated Mass spectrum doesn t depend on initial phase (but broader peak shape) To increase resolution absorption-spectra are used: This requires an initial phase of degrees (a) for all ions Synchronization of ion injection in the Orbitrap and start of transient acquisition (determination of t ) is required Hardware optimization See aslo ASMS Poster MP93 Oliver Lange et al. 8

9 Advanced Signal Processing - working principle Injection of ions into the Orbitrap: At time t all the ions are injected at (nearly) identical phase φ and start oscillating Recorded transient starts close to this point t but not exactily (ToF, electronics delay and jitter) OE-1 Deflector C-trap CE Lenses OE-2 In order to make use of the inner information these starting conditions must be known with high accuracy (tens of nanoseconds) If multiple ions are present, the parameters t and φ can be found out by matching 9 See ASMS poster: M93 Lange et al., Enhanced FT for Orbitrap Mass Spectrometry

10 Fast stabilization of the transient Relative Intensity Previous set up 8.9 ms ms m/z Relative Intensity <.6 ms New set up ms m/z 1

11 Advanced Signal Processing on the Orbitrap Elite Advanced Signal Processing ON R= R= R= R= R= R= R= m/z Advanced Signal Processing OFF R= R= R=64816 R= R= R= R= m/z m/z Intact Yeast Enolase (46.64 kda), 47+ ion, 768 ms transients 11 (P<1*1-1 Torr)

12 Intact protein analysis: Yeast Enolase (46.64 kda) Relative Abundance Orbitrap Elite R= R= R= R= R= R= R=9935 R= R= R= R= R= R= R= R= R= R=4234 R= R=3244 Yeast Enolase, 47+ is baseline resolved on Orbitrap Elite. At 47+ charge state, the mass difference between isotopic peaks is only.21 Th! LTQ Orbitrap Velos m/z 12 ASMS 211, MP92: Increased Analytical Performance on a Hybrid Linear Ion Trap-FTMS Mass Spectrometer with a High-Field Orbitrap Mass Analyzer, Martin Zeller, Catharina Crone, Mathias Mueller, Eugen Damoc, Eduard Denisov, Alexander Makarov, Dirk Nolting, and Thomas Moehring

13 Orbitrap Elite: Schematics Faster Dual trap electronics: >12 scans/sec More robust interface optics Compact high-field Orbitrap analyzer+ eft 13

14 Orbitrap Elite: Major Improvement in Scan Speed Orbitrap Velos 1 Hz Relative Abundance Time (min) Orbitrap Elite 4 Hz Relative Abundance Time (min) Orbitrap Velos R 68, Relative Abundance R= R= R= m/z Orbitrap Elite R 64, Relative Abundance R= R= R= R= R= R= R= m/z 14

15 MS scan speed at different resolutions MS scan speed (Hz) k Orbitrap Elite Orbitrap Velos Pro m/z 4 24, at 1 Hz: 6, at 4 Hz: 15, at 8 Hz: Exploring new possibilities Exploiting the faster acquisition rate Increasing HCD scan speed 15

16 Orbitrap Elite vs. LTQ Orbitrap Velos: Cycle times for TOP1 HCD experiment 192_48_eFTon_ 1ms_delay_ /12/21 5:25:37 PM LTQ Orbitrap Velos LTQ Orbitrap Velos LTQ Orbitrap Velos Orbitrap Elite Relative Abundance Relative Abundance Relative Abundance Relative Abundance Time (min) s Time (min) Time (min) s 2.9 s 2.48 s NL: 1.62E8 TIC MS calmix_ddhcdto p1_mrfa_15 _2ms_MS_1ms _MS2 NL: 1.62E8 TIC MS calmix_ddhcdtop1 _MRFA_75_2ms_ MS_1ms _MS2_ NL: 1.5E8 67 TIC MS calmix_ddhcdtop1 _MRFA_75_2m s_ms_1ms_ms2_3 k_ftms Time (min) NL: 5.23E8 TIC MS 192_48_eFTo n_1ms_delay _ *Calmix, MS: 1e6 ions, IT~2 ms; MS2: 2e4 ions, IT~1 ms with pagc

17 Orbitrap Elite: The New Performance Standard New High Field Orbitrap provides unprecedented mass resolution Technology Breakthroughs: New High Field Orbitrap Resolving power of >24, 4X scan speed improvement Advanced signal processing New 7 Hz HCD capability New pre-amplifier Multiple fragmentation techniques for comprehensive peptide and protein ID; unambiguous small molecule identification Ultimate in top down protein analysis A quantum leap in ultra high resolution and accurate mass analysis 17

18 Technology update 211 Q Exactive Quanfirmation identify, quantify, confirm

19 New Q-Exactive Features Instrument Layout Ion source Predictive Quadrupole C-trap Multiple automatic S-lens directly mass fills for interfaced filter gain for spectrum higher control, increased to transmission multiplexing HCD parallel sensitivity, filling increases with & increases rugged selectivity, detection spectrum optics duty true quality more cycle MS/MS speed Advanced signal processing for Orbitrap data speed & resolution increase 19

20 What do we gain by selected ion monitoring? N= Full MS S/N = 745 NL: 1.94E8 [15.-2.] Lowest detected signal/scan Relative Abundance N= SIM (1amu) S/N = 54 NL: 1.12E8 [ ] Lowest detected signal/scan Gain in sensitivity (7x) S/N (spectrum) Caffeine S/N (FMS) S/N (SIM1) Sensitivity gain 5 1 x with SIM mode 2

21 Spectrum multiplexing: principle of operation Standard operation mode vs Spectrum multiplexing 5.x x1 8 4.x x1 8 Ion count (arb.) 3.x x1 8 2.x x1 8 1.x1 8 m/z m/z262.6 m/z393.2 m/z m/z1622. C-Trap storage: No ion loss over a broad range of storage times! (St.Steel, fused silica, ceramics) 5.x Inject time [ms] 21

22 Q Exactive: Redefining Qual/Quan Workflows Trace-level metabolites, contaminants, peptides/proteins in complex mixtures Quanfirmation TM : Simultaneously identify, quantify and confirm in one analytical run Resolving power of >14, New high performance quadrupole parent ion selection capability Advanced signal processing for faster scanning New S Lens ion optics 3X sensitivity Multiple ion filling technologies for faster analysis 1 Hz HCD MS/MS Exceptional MS/MS sensitivity, data quality and quantitation 22

23 What s next?

24 What to expect from Orbitrap mass spectrometry? Faster and more powerful Orbitraps High-field Orbitraps (dimensions, voltage) Higher-performance (resolving power, mass accuracy) Orbitraps Improved data processing methods Dynamic Range/sensitivity (Higher space charge capacity C-trap) Intelligent is better than fast : multiple fills allow to improve quality of analysis 24

25 Resolving power ~ R 1,, EpiT21_ _1uscan_5e4_3sec #1 RT:.1 AV: 1 NL: 9.64E7 T: FTMS + p ESI SIM ms [ ] R=1185 z= Relative Abundance R=11824 z= R=1981 z= m/z 25 Research only! Martin Zeller

26 Summary: Science Community Applications Technology Products New & Events... Happenings at Thermo Fisher and Scientific Community 26

27 Acknowledgements San Jose, CA: E. Hemenway M. Antonczak M. Senko J. Syka J. Schwartz V. Zabrouskov I. Jardine T. Ziberna J-J. Dunyach E. Wouters P. Remes D. Taylor B. Tehlirian V. Kovtoun N. Izgarian P. Fong J. Gabel S. Fenske P. Cardenas M. Konicek R. Hermezian W. Dewey B. Siebert P. Atherton M. Ahrens J. Sklenar S. Zanon W. Wang A. Specht J. Horner J. Blethrow H. Bui Bremen, DE: E. Denisov A. Kholomeev W. Balschun O. Lange K. Strupat S. Horning R. Pesch J. Srega G. Jung W. Huels F. Czemper O. Hengelbrock A. Wieghaus J. Griep-Raming E. Schroeder U. Froehlich D. Nolting R. Malek M. Kellmann M. Zeller S. Strube S. Moehring R. Seedorf S. Kanngiesser 27

28 28 Thank you for your attention

29 29 Q Exactive: Instrument model