Advanced Column Designs and Innovative Packing Procedures

Transcription

1 Advanced Column Designs and Innovative Packing Procedures The Perfect Combination for Preparative and Process HPLC Separations 2002 Product Catalog

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3 MODcol Yesterday and Today Founded in 1982 by Dr. Yehuda Shalon, MODcol is a leading provider of stainless steel columns and packing services for high performance preparative liquid chromatography. Early on, the company s modular column concept and wide selection of column sizes, diameters and lengths were praised for their high level of performance. Continued innovation has led to a number of patents being issued for our products. MODcol is renowned for providing the chromatography community with high performance chromatographic column packing services for 20 years. As a result of the experience that MODcol has garnered we succeed where others fail, giving us a unique advantage in the chromatography market. We know that there are no secrets or gimmicks behind manufacturing high quality preparative chromatography columns. The best columns combine high quality hardware, specialized packing technology and high quality media to yield columns that provide the highest level of LC performance, stability and longevity. Following this philosophy, MODcol offers a range of chromatography column products that provide high performance solutions that suit your needs. Today, MODcol is building on its reputation for high quality packed columns with its novel Spring Column and MultiPacker products. These products offer advancements to the modern chromatographer in terms of chromatographic performance, reproducibility and dependability. MODcol is poised at the forefront of column technology with new and novel ideas from the Research and Development department located in its Morgan Hill California headquarters. Look to MODcol to solve your difficult problems. Our new facility in Morgan Hill.

4 A Brief Overview of MODcol s Product Lines MODcol pioneered Axial Compression Spring Columns, a novel concept for preparative HPLC chromatography that features a powerful self-contained axial compression mechanism. As the volume occupied by the media gradually contracts, this mechanism expands to maintain a high density in the packed column, yielding high efficiency and increasing column lifetime. The MultiPacker is an instrument for on-site slurry packing of Spring Columns. This instrument packs Spring Columns in only a few minutes, start to finish. The MultiPacker and Spring Column are designed as separate units. Once a column is packed, the MultiPacker is immediately ready to pack another column while the packed Spring Column is relocated to the point of use. Used together, the Spring Column and MultiPacker provide a dramatic cost savings over conventional dedicated packing systems. MODcol s Patented Fixed Bed Flanged Chromatography Columns are widely accepted as the standard for high performance prep-lc. MODcol s patented flow distribution system provides uniform flow across the top of the media bed at the column inlet and outlet, yielding analytical level performance in a prep-scale column. MODcol Industrial-Size Fixed-Bed Columns are custom designed and manufactured for the pharmaceutical and biotechnology industries in sizes from 150 mm (6 in) up to 600 mm (24 in) internal diameter and are offered with pressure ratings from 100psi to over 2000psi. MODcol process and industrial scale columns are based on the patented design of the preparative and semi-preparative fixed bed column products completing our family of columns that stand ready to serve your high-performance liquid chromatography scale-up needs. MODcol Value-Line Threaded Columns are a non-repackable threaded design that offers high performance for the value-conscious chromatographer. These columns are offered in 10mm, 20mm and 30mm internal diameters. MODcol s Slurry-Packing Service sets us apart from other chromatography companies. Our experience in this area enables us to solve problems better than any other column manufacturer in the market today. MODcol s expertise has been built from 20 years of specialized column packing, with thousands of preparative HPLC fixed bed columns packed to date. MODcol s success and efficiency in slurry packing is due to the in-house proprietary procedures that have been developed and refined over the years. Our know-how makes it possible for us, in many instances, to achieve packing efficiencies beyond the media manufacturer s expectations. Bulk Media offered by MODcol was selected by drawing on our extensive experience in packing media from all players in the preparatory market. We are proud to offer three media product lines that balance the purity, performance and economy required for cutting-edge preparative chromatographic separations. The trio of MODsil, Kromasil and Daiso represent the finest quality media available today with their excellent level of performance, high capacity and robust mechanical properties. Using our proprietary methods for slurry packing, we deliver these medias as high quality packed columns with a high level of economy.

5 Table of Contents Product Spotlight: Axial Compression Spring Columns Product Spotlight: Fixed Bed Flanged Columns Bulk Media MODsil Kromasil DaisoGel Protein and Peptide Purification Media Prepacked Flanged and Spring Columns MODsil C MODSil C MODSil C MODSil Silica Kromasil C Kromasil C Kromasil C Kromasil Silica Daiso C Daiso C Daiso C Daiso Silica Value-Line Threaded Chromotography Columns MODsil C MODsil C MODsil C MODsil Silica Kromasil C Kromasil C Kromasil C Kromasil Silica Daiso C Daiso C Daiso C Daiso Silica Application-Specific Prepacked Columns Columns for Chiral Chromatography Columns for Super-Critical-Fluid Chromatography Columns for Rapid and High-Throughput Separations Columns for Purification of Peptides and Proteins MultiPacker Instruments Axial Compression Spring Columns Columns for Process-Scale Separations Parts and Accessories Slurry Packing Services for Flanged Columns Slurry Packing Services for Spring Columns General Conversion Charts and Tables Ordering Information inside back cover

6 Product Spotlight: Axial Compression Spring Columns The Spring Column products are MODcol s highest performance chromatography columns. Spring Columns have an integrated axial compression system that delivers high performance in a small and portable package. Axial Compression Spring technology separates the axial compression that is used during column packing (requiring a large displacement) from the axial compression used during column use (requiring a small displacement). This results in a lighter, smaller and less expensive axial compression system that is fully contained within the body of the Spring Column. Fail-safe and fault-tolerant operation is ensured by the uncomplicated, purely mechanical design of the axial compression system. Some products available today from other manufacturers are continually powered by bulky hydraulic systems that must be mounted on a cart and some require tedious manual actuation during packing while providing only marginal or unreliable performance. In contrast, MODcol s Axial Compression Spring system provides industry-leading chromatographic performance in an easy-to-use package. The Technology Behind the Spring Column All axial compression systems compensate for void formation that can occur under certain circumstances commonly found in preparative-scale chromatography columns. Typically, a movable piston defines the end of the column at which the inlet flow is located. A force is continuously used to push the piston against the bed of the packed column. The force used to push the piston against the bed may be active or passive, mechanical or hydraulic. This action allows the piston to maintain contact with the bed when it consolidates or when particles are physically degraded and washed out of the column. In traditional columns designed with fixed bed geometry, bed consolidation can lead to an internal void or an uneven bed at the inlet side causing peak tailing, broadening or splitting. Such severe performance degradation renders the column useless for high performance separations. In general, axial compression systems must compensate for void formation as discussed earlier and a careful consideration of the void volume can be used to show that a spring driven system is well suited for this task. Most columns used in modern preparative chromatography are 25cm and shorter in length. Even the worst modern chromatographic resin will not consolidate more than five to ten percent in a packed bed. At these high levels of bed settling, other problems such as frit blockage or extensive media collapse would render the column unusable. It is therefore sufficient to compensate for voids on the order of one centimeter in length, roughly equal to five or ten percent of the bed length. Over this length, a spring can be used to provide the force necessary for axial compression. A spring-driven axial compression system does not operate in the same manner as a hydraulic powered one. In a spring, the restorative force increases from zero as the spring is compressed. In order for the spring to perform useful work it must be under a minimum amount of compression at all times. To advance the piston, the force generated by the spring must be greater than the static frictional forces. The spring force must also be sufficient to counteract the hydrostatic force that is generated by the pressure of the liquid within the column acting upon the piston head. The power of axial compression fits in the palm of your hand when you use MODcol s Spring Columns. Spring Column principle of operation. 2

7 The limits discussed in the previous paragraph define a window of operation for the spring. Consider the figure below. Illustration of force vs. compression relationship for a spring. When the spring is fully compressed, the force is greatest indicated by Lmin, Fmax. In the spring column design, one end of the spring is locked in place while the other is free to push against the piston. As the spring uncompresses to push the piston along the column, the force it is generating decreases. When the force is equal to the hydrostatic force in the column plus the static frictional force, the spring can no longer advance the piston and the axial compression action is lost. This is indicated by L0,F0 on the graph. If the hydrostatic pressure is reduced to zero, i.e. the pump is shut off, the piston could advance but when the pump was restarted the piston could be pushed back to L0. This situation is easily overcome by returning the column to the MultiPacker instrument and applying hydraulic pressure to recompress the spring back to Lmin, Fmax. Experience has proven that, under most real-world conditions, the Spring Column provides dramatically improved performance over similar fixed bed columns. This improvement occurs in both performance and longevity. In addition, Spring Column performance is very reproducible from column to column when compared to fixed bed columns. This translates to dependable high performance at little additional cost. MODcol Spring Columns are available for use with the MultiPacker column-packing instrument for users who would like to pack the columns on site using their own media and/or procedures. The Spring Column is also available as a ready-to-use packed column, a feature that is unique among axial compression columns on the market today. The columns used above were 50mm ID Spring Columns packed to a bed depth of 15cm using 20µ Chiracel OD. Each column was packed only once. 3

8 Product Spotlight: Fixed Bed Flanged Columns MODcol s high quality fixed bed flanged column hardware has been awarded several patents for its design. All of our flanged column products are constructed from a high quality 316 stainless steel alloy that is electro-polished to provide the highest possible corrosion protection and chemical inertness. The flanged construction is designed to be rugged and is very conservatively rated at 2000psi. The column closure is achieved using a set of highstrength bolts and two heavy-duty endplates that are engineered to provide a long product lifetime. The simplicity of this design, without threads or other parts that can wear out, makes the column hardware practically infinitely reusable and repackable. Central to the ability to provide near-analytical levels of performance in preparative scale columns is the flow distribution system. MODcol s column designs promote homogeneous flow across the boundaries of the media bed to reduce band broadening. Pure Teflon seals provide maximum inertness and solvent compatibility under all conditions. All frits used in MODcol columns are made by sintering stainless steel powder into a porous disc. These critical parts are sourced from suppliers who provide this material with a high open area (area available for flow) because this ensures a negligible pressure drop across the frit. Special care is taken to passivate the internal surfaces of the frits so that they are inert towards biological materials. MODcol fixed bed columns are offered in a variety of inner diameters and lengths to suit a wide range of needs. We feature these columns in all of our packed column products for the highest level of performance and reliability. When your high performance separation requires nothing but the best, let MODcol flanged columns lead the way. Flanged column product line. 4

9 Bulk Media MODcol offers three lines of media that balance the purity, performance and economy required for cutting edge preparative chromatographic separations. Each media brand represents an excellent choice for high performance separations. MODsil MODsil is premium spherical silica packing material that is designed to meet the highest HPLC or SMB needs from preparative to process scale. MODsil was developed to be the perfect complement to the MODcol s extremely successful packing technology and outstanding performance is achieved in all column sizes. MODsil packing material is fully end capped with high surface area and mechanical strength. It is ideally suited for separation of peptides and other medium sized molecules. Due to its high purity, it separates basic compounds well. For a selection of prepacked, ready-to-use columns featuring MODsil media, please consult pages 7-10 and Bulk media product line. 8 µm, 100Å 10 µm, 100Å 12 µm, 100Å C18 MM MM MM C08 MM MM MM C04 MM MM MM Bare Silica MMSI MMSI MMSI Kromasil The renowned performance of Kromasil spherical silica results from the combination of: High surface area High mechanical strength Chemical purity and bonding stability Optimized surface properties and a well-defined pore structure This media features a narrow particle size distribution for high efficiency, lowpressure drop and high-throughput. Kromasil is a time-proven performer in a variety of applications from basic peptide separations to acidic natural product purifications. A high level of quality control and a reputation of excellence are associated with Kromasil media. Use it with confidence in your most demanding applications. We offer 7µm and 10µm particle sizes and 100Å pore diameters, however other particle sizes and porosities are available upon request. For a selection of prepacked, ready-to-use columns featuring Kromasil media, please consult pages and µm, 100Å 10µum, 100Å C18 MM MM C08 MM MM C04 MM MM Bare Silica MMSI MMSI

10 Bulk Media DaisoGel Daiso s SP-P (spherical ultra pure) series is based on a spherical and totally porous silica gel with a low level of metal impurities (specified to be less than 10 ppm each for Al, Fe, Ti and Zr). This packing material also features a narrow particle size distribution, high loading capacity and enhanced mechanical strength. DaisoGel is a workhorse, production-grade media for large-scale applications. Even with such advanced features it represents a very cost effective media for all preparative applications. We offer 10µm, 15µm and 20µm particle sizes and 120Å pore diameters, however other particle sizes and porosities are available upon request. For a selection of prepacked, ready-to-use columns featuring DaisoGel media, please consult pages and µm, 120Å 15 µm, 120Å 20 µm, 120Å C18 MM MM MM C08 MM MM MM C04 MM MM MM Bare Silica MMSI MMSI MMSI Protein and Peptide Purification Media MODcol has selected two media for this demanding application: the Vydac 300Å and the Daiso 300Å, both exhibiting very good separations and complementary selectivity. The selection of 300Å pore is due to the fact that larger polypeptides must enter the pore interior to interact with the majority of the stationary phase. This allows separations that are not possible when separating large polypeptides on media having 100Å pores. At the same time, wide pore silica are well-suited for the separation of small peptides. DAISO 10 µm, 300Å 15µm, 300Å C18 MM MM C08 MM MM C04 MM MM VYDAC 10-15µm, 300Å 15-20µm, 300Å C18 MM MM C08 MM MM C04 MM MM

11 Prepacked Flanged and Spring Columns The Perfect Combination Using our proprietary methods for slurry packing, we are able to supply impressive results with virtually any LC media on the market today. We know that to obtain the best packed column, the packing conditions must be tailored to each media a time consuming, difficult and expensive task. We have chosen to offer MODsil, Kromasil and DaisoGel media because of the excellent level of performance and reliability they demonstrate. By eliminating packing development, you get high quality products with a high level of economy. MODsil C18 Flanged Columns Prepacked flanged column product line. Column Size 8 µm, 100Å 10 µm, 100Å 12 µm, 100Å 12 mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 50 cm FP FP FP Spring Columns* Column Size 8 µm, 100Å 10 µm, 100Å 12 µm, 100Å 25 mm ID x 10 cm SP SP SP mm ID x 15 cm SP SP SP mm ID x 25 cm SP SP SP mm ID x 10 cm SP SP SP mm ID x 15 cm SP SP SP mm ID x 25 cm SP SP SP Analytical Columns Column Size 8 µm, 100Å 10 µm, 100Å 12 µm, 100Å 4.6 mm ID x 25 cm FP FP FP * Prepacked Spring Columns are not user-serviceable and must be returned to MODcol for repacking. 7

12 Prepacked Flanged and Spring Columns MODsil C 8 Flanged Columns Column Size 8 µm, 100Å 10 µm, 100Å 12 µm, 100Å 12 mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 50 cm FP FP FP Spring Columns* Column Size 8 µm, 100Å 10 µm, 100Å 12 µm, 100Å 25 mm ID x 10 cm SP SP SP mm ID x 15 cm SP SP SP mm ID x 25 cm SP SP SP mm ID x 10 cm SP SP SP mm ID x 15 cm SP SP SP mm ID x 25 cm SP SP SP Analytical Columns Column Size 8 µm, 100Å 10 µm, 100Å 12 µm, 100Å 4.6 mm ID x 25 cm FP FP FP * Prepacked Spring Columns are not user-serviceable and must be returned to MODcol for repacking. 8

13 Prepacked Flanged and Spring Columns MODsil C 4 Flanged Columns Column Size 8 µm, 100Å 10 µm, 100Å 12 µm, 100Å 12 mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 50 cm FP FP FP Spring Columns* Column Size 8 µm, 100Å 10 µm, 100Å 12 µm, 100Å 25 mm ID x 10 cm SP SP SP mm ID x 15 cm SP SP SP mm ID x 25 cm SP SP SP mm ID x 10 cm SP SP SP mm ID x 15 cm SP SP SP mm ID x 25 cm SP SP SP Analytical Columns Column Size 8 µm, 100Å 10 µm, 100Å 12 µm, 100Å 4.6 mm ID x 25 cm FP FP FP * Prepacked Spring Columns are not user-serviceable and must be returned to MODcol for repacking. 9

14 Prepacked Flanged and Spring Columns MODsil Silica Flanged Columns Column Size 8 µm, 100Å 10 µm, 100Å 12 µm, 100Å 12 mm ID x 5 cm FPSI FPSI FPSI mm ID x 10 cm FPSI FPSI FPSI mm ID x 15 cm FPSI FPSI FPSI mm ID x 25 cm FPSI FPSI FPSI mm ID x 5 cm FPSI FPSI FPSI mm ID x 10 cm FPSI FPSI FPSI mm ID x 15 cm FPSI FPSI FPSI mm ID x 25 cm FPSI FPSI FPSI mm ID x 5 cm FPSI FPSI FPSI mm ID x 10 cm FPSI FPSI FPSI mm ID x 15 cm FPSI FPSI FPSI mm ID x 25 cm FPSI FPSI FPSI mm ID x 5 cm FPSI FPSI FPSI mm ID x 10 cm FPSI FPSI FPSI mm ID x 15 cm FPSI FPSI FPSI mm ID x 25 cm FPSI FPSI FPSI mm ID x 5 cm FPSI FPSI FPSI mm ID x 10 cm FPSI FPSI FPSI mm ID x 15 cm FPSI FPSI FPSI mm ID x 25 cm FPSI FPSI FPSI mm ID x 25 cm FPSI FPSI FPSI mm ID x 25 cm FPSI FPSI FPSI mm ID x 50 cm FPSI FPSI FPSI Spring Columns* Column Size 8 µm, 100Å 10 µm, 100Å 12 µm, 100Å 25 mm ID x 10 cm SPSI SPSI SPSI mm ID x 15 cm SPSI SPSI SPSI mm ID x 25 cm SPSI SPSI SPSI mm ID x 10 cm SPSI SPSI SPSI mm ID x 15 cm SPSI SPSI SPSI mm ID x 25 cm SPSI SPSI SPSI Analytical Columns Column Size 8 µm, 100Å 10 µm, 100Å 12 µm, 100Å 4.6 mm ID x 25 cm FPSI FPSI FPSI * Prepacked Spring Columns are not user-serviceable and must be returned to MODcol for repacking. 10

15 Prepacked Flanged and Spring Columns Kromasil C18 Flanged Columns Column Size 7 µm, 100Å 10 µm, 100Å 12 mm ID x 5 cm FP FP mm ID x 10 cm FP FP mm ID x 15 cm FP FP mm ID x 25 cm FP FP mm ID x 5 cm FP FP mm ID x 10 cm FP FP mm ID x 15 cm FP FP mm ID x 25 cm FP FP mm ID x 5 cm FP FP mm ID x 10 cm FP FP mm ID x 15 cm FP FP mm ID x 25 cm FP FP mm ID x 5 cm FP FP mm ID x 10 cm FP FP mm ID x 15 cm FP FP mm ID x 25 cm FP FP mm ID x 5 cm FP FP mm ID x 10 cm FP FP mm ID x 15 cm FP FP mm ID x 25 cm FP FP mm ID x 25 cm FP FP mm ID x 25 cm FP FP mm ID x 50 cm FP FP Spring Columns* Column Size 7 µm, 100Å 10 µm, 100Å 25 mm ID x 10 cm SP SP mm ID x 15 cm SP SP mm ID x 25 cm SP SP mm ID x 10 cm SP SP mm ID x 15 cm SP SP mm ID x 25 cm SP SP Analytical Columns Column Size 7 µm, 100Å 10 µm, 100Å 4.6 mm ID x 25 cm FP FP * Prepacked Spring Columns are not user-serviceable and must be returned to MODcol for repacking. 11

16 Prepacked Flanged and Spring Columns Kromasil C 8 Flanged Columns Column Size 7 µm, 100Å 10 µm, 100Å 12 mm ID x 5 cm FP FP mm ID x 10 cm FP FP mm ID x 15 cm FP FP mm ID x 25 cm FP FP mm ID x 5 cm FP FP mm ID x 10 cm FP FP mm ID x 15 cm FP FP mm ID x 25 cm FP FP mm ID x 5 cm FP FP mm ID x 10 cm FP FP mm ID x 15 cm FP FP mm ID x 25 cm FP FP mm ID x 5 cm FP FP mm ID x 10 cm FP FP mm ID x 15 cm FP FP mm ID x 25 cm FP FP mm ID x 5 cm FP FP mm ID x 10 cm FP FP mm ID x 15 cm FP FP mm ID x 25 cm FP FP mm ID x 25 cm FP FP mm ID x 25 cm FP FP mm ID x 50 cm FP FP Spring Columns* Column Size 7 µm, 100Å 10 µm, 100Å 25 mm ID x 10 cm SP SP mm ID x 15 cm SP SP mm ID x 25 cm SP SP mm ID x 10 cm SP SP mm ID x 15 cm SP SP mm ID x 25 cm SP SP Analytical Columns Column Size 7 µm, 100Å 10 µm, 100Å 4.6 mm ID x 25 cm FP FP * Prepacked Spring Columns are not user-serviceable and must be returned to MODcol for repacking. 12

17 Prepacked Flanged and Spring Columns Kromasil C 4 Flanged Columns Column Size 7 µm, 100Å 10 µm, 100Å 12 mm ID x 5 cm FP FP mm ID x 10 cm FP FP mm ID x 15 cm FP FP mm ID x 25 cm FP FP mm ID x 5 cm FP FP mm ID x 10 cm FP FP mm ID x 15 cm FP FP mm ID x 25 cm FP FP mm ID x 5 cm FP FP mm ID x 10 cm FP FP mm ID x 15 cm FP FP mm ID x 25 cm FP FP mm ID x 5 cm FP FP mm ID x 10 cm FP FP mm ID x 15 cm FP FP mm ID x 25 cm FP FP mm ID x 5 cm FP FP mm ID x 10 cm FP FP mm ID x 15 cm FP FP mm ID x 25 cm FP FP mm ID x 25 cm FP FP mm ID x 25 cm FP FP mm ID x 50 cm FP FP Spring Columns* Column Size 7 µm, 100Å 10 µm, 100Å 25 mm ID x 10 cm SP SP mm ID x 15 cm SP SP mm ID x 25 cm SP SP mm ID x 10 cm SP SP mm ID x 15 cm SP SP mm ID x 25 cm SP SP Analytical Columns Column Size 7 µm, 100Å 10 µm, 100Å 4.6 mm ID x 25 cm FP FP * Prepacked Spring Columns are not user-serviceable and must be returned to MODcol for repacking. 13

18 Prepacked Flanged and Spring Columns Kromasil Silica Flanged Columns Column Size 7 µm, 100Å 10 µm, 100Å 12 mm ID x 5 cm FPSI FPSI mm ID x 10 cm FPSI FPSI mm ID x 15 cm FPSI FPSI mm ID x 25 cm FPSI FPSI mm ID x 5 cm FPSI FPSI mm ID x 10 cm FPSI FPSI mm ID x 15 cm FPSI FPSI mm ID x 25 cm FPSI FPSI mm ID x 5 cm FPSI FPSI mm ID x 10 cm FPSI FPSI mm ID x 15 cm FPSI FPSI mm ID x 25 cm FPSI FPSI mm ID x 5 cm FPSI FPSI mm ID x 10 cm FPSI FPSI mm ID x 15 cm FPSI FPSI mm ID x 25 cm FPSI FPSI mm ID x 5 cm FPSI FPSI mm ID x 10 cm FPSI FPSI mm ID x 15 cm FPSI FPSI mm ID x 25 cm FPSI FPSI mm ID x 25 cm FPSI FPSI mm ID x 25 cm FPSI FPSI mm ID x 50 cm FPSI FPSI Spring Columns* Column Size 7 µm, 100Å 10 µm, 100Å 25 mm ID x 10 cm SPSI SPSI mm ID x 15 cm SPSI SPSI mm ID x 25 cm SPSI SPSI mm ID x 10 cm SPSI SPSI mm ID x 15 cm SPSI SPSI mm ID x 25 cm SPSI SPSI Analytical Columns Column Size 7 µm, 100Å 10 µm, 100Å 4.6 mm ID x 25 cm FPSI FPSI * Prepacked Spring Columns are not user-serviceable and must be returned to MODcol for repacking. 14

19 Prepacked Flanged and Spring Columns Daiso C18 Flanged Columns Column Size 10 µm, 120Å 15 µm, 120Å 20 µm, 120Å 12 mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 50 cm FP FP FP Spring Columns* Column Size 10 µm, 120Å 15 µm, 120Å 20 µm, 120Å 25 mm ID x 10 cm SP SP SP mm ID x 15 cm SP SP SP mm ID x 25 cm SP SP SP mm ID x 10 cm SP SP SP mm ID x 15 cm SP SP SP mm ID x 25 cm SP SP SP Analytical Columns Column Size 10 µm, 120Å 15 µm, 120Å 20 µm, 120Å 4.6 mm ID x 25 cm FP FP FP * Prepacked Spring Columns are not user-serviceable and must be returned to MODcol for repacking. 15

20 Prepacked Flanged and Spring Columns Daiso C 8 Flanged Columns Column Size 10 µm, 120Å 15 µm, 120Å 20 µm, 120Å 12 mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 50 cm FP FP FP Spring Columns* Column Size 10 µm, 120Å 15 µm, 120Å 20 µm, 120Å 25 mm ID x 10 cm SP SP SP mm ID x 15 cm SP SP SP mm ID x 25 cm SP SP SP mm ID x 10 cm SP SP SP mm ID x 15 cm SP SP SP mm ID x 25 cm SP SP SP Analytical Columns Column Size 10 µm, 120Å 15 µm, 120Å 20 µm, 120Å 4.6 mm ID x 25 cm FP FP FP * Prepacked Spring Columns are not user-serviceable and must be returned to MODcol for repacking. 16

21 Prepacked Flanged and Spring Columns Daiso C 4 Flanged Columns Column Size 10 µm, 120Å 15 µm, 120Å 20 µm, 120Å 12 mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 5 cm FP FP FP mm ID x 10 cm FP FP FP mm ID x 15 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 25 cm FP FP FP mm ID x 50 cm FP FP FP Spring Columns* Column Size 10 µm, 120Å 15 µm, 120Å 20 µm, 120Å 25 mm ID x 10 cm SP SP SP mm ID x 15 cm SP SP SP mm ID x 25 cm SP SP SP mm ID x 10 cm SP SP SP mm ID x 15 cm SP SP SP mm ID x 25 cm SP SP SP Analytical Columns Column Size 10 µm, 120Å 15 µm, 120Å 20 µm, 120Å 4.6 mm ID x 25 cm FP FP FP * Prepacked Spring Columns are not user-serviceable and must be returned to MODcol for repacking. 17

22 Prepacked Flanged and Spring Columns Daiso Silica Flanged Columns Column Size 10 µm, 120Å 15 µm, 120Å 20 µm, 120Å 12 mm ID x 5 cm FPSI FPSI FPSI mm ID x 10 cm FPSI FPSI FPSI mm ID x 15 cm FPSI FPSI FPSI mm ID x 25 cm FPSI FPSI FPSI mm ID x 5 cm FPSI FPSI FPSI mm ID x 10 cm FPSI FPSI FPSI mm ID x 15 cm FPSI FPSI FPSI mm ID x 25 cm FPSI FPSI FPSI mm ID x 5 cm FPSI FPSI FPSI mm ID x 10 cm FPSI FPSI FPSI mm ID x 15 cm FPSI FPSI FPSI mm ID x 25 cm FPSI FPSI FPSI mm ID x 5 cm FPSI FPSI FPSI mm ID x 10 cm FPSI FPSI FPSI mm ID x 15 cm FPSI FPSI FPSI mm ID x 25 cm FPSI FPSI FPSI mm ID x 5 cm FPSI FPSI FPSI mm ID x 10 cm FPSI FPSI FPSI mm ID x 15 cm FPSI FPSI FPSI mm ID x 25 cm FPSI FPSI FPSI mm ID x 25 cm FPSI FPSI FPSI mm ID x 25 cm FPSI FPSI FPSI mm ID x 50 cm FPSI FPSI FPSI Spring Columns* Column Size 10 µm, 120Å 15 µm, 120Å 20 µm, 120Å 25 mm ID x 10 cm SPSI SPSI SPSI mm ID x 15 cm SPSI SPSI SPSI mm ID x 25 cm SPSI SPSI SPSI mm ID x 10 cm SPSI SPSI SPSI mm ID x 15 cm SPSI SPSI SPSI mm ID x 25 cm SPSI SPSI SPSI Analytical Columns Column Size 10 µm, 120Å 15 µm, 120Å 20 µm, 120Å 4.6 mm ID x 25 cm FPSI FPSI FPSI * Prepacked Spring Columns are not user-serviceable and must be returned to MODcol for repacking. 18

23 Value-Line Threaded Chromatography Columns MODcol recognizes the need for an alternative to the flanged column for chromatographers who need a high-performance solution that represents a good value. MODcol s threaded columns provide excellent performance but are not designed to be repackable. These columns are offered with inner diameters of 10mm, 20mm and 30mm to suit many of the modern preparative chromatographer s needs. MODcol threaded columns are offered prepacked with MODsil, Kromasil and Daiso media that are well suited for a broad range of chromatographic separations. Combined with the economy of the threaded column package, this represents a true value. Value-line products. MODsil C18 Column Size 8 µm, 100Å 10 µm, 100Å 12 µm, 100Å 10 mm ID x 5 cm TP TP TP mm ID x 10 cm TP TP TP mm ID x 15 cm TP TP TP mm ID x 25 cm TP TP TP mm ID x 5 cm TP TP TP mm ID x 10 cm TP TP TP mm ID x 15 cm TP TP TP mm ID x 25 cm TP TP TP mm ID x 5 cm TP TP TP mm ID x 10 cm TP TP TP mm ID x 15 cm TP TP TP mm ID x 25 cm TP TP TP MODsil C 8 Column Size 8 µm, 100Å 10 µm, 100Å 12 µm, 100Å 10 mm ID x 5 cm TP TP TP mm ID x 10 cm TP TP TP mm ID x 15 cm TP TP TP mm ID x 25 cm TP TP TP mm ID x 5 cm TP TP TP mm ID x 10 cm TP TP TP mm ID x 15 cm TP TP TP mm ID x 25 cm TP TP TP mm ID x 5 cm TP TP TP mm ID x 10 cm TP TP TP mm ID x 15 cm TP TP TP mm ID x 25 cm TP TP TP MODsil C 4 Column Size 8 µm, 100Å 10 µm, 100Å 12 µm, 100Å 10 mm ID x 5 cm TP TP TP mm ID x 10 cm TP TP TP mm ID x 15 cm TP TP TP mm ID x 25 cm TP TP TP mm ID x 5 cm TP TP TP mm ID x 10 cm TP TP TP mm ID x 15 cm TP TP TP mm ID x 25 cm TP TP TP mm ID x 5 cm TP TP TP mm ID x 10 cm TP TP TP mm ID x 15 cm TP TP TP mm ID x 25 cm TP TP TP

24 Value-Line Threaded Chromatography Columns MODsil Silica Column Size 8 µm, 100Å 10 µm, 100Å 12 µm, 100Å 10 mm ID x 5 cm TPSI TPSI TPSI mm ID x 10 cm TPSI TPSI TPSI mm ID x 15 cm TPSI TPSI TPSI mm ID x 25 cm TPSI TPSI TPSI mm ID x 5 cm TPSI TPSI TPSI mm ID x 10 cm TPSI TPSI TPSI mm ID x 15 cm TPSI TPSI TPSI mm ID x 25 cm TPSI TPSI TPSI mm ID x 5 cm TPSI TPSI TPSI mm ID x 10 cm TPSI TPSI TPSI mm ID x 15 cm TPSI TPSI TPSI mm ID x 25 cm TPSI TPSI TPSI Kromasil C18 Column Size 7 µm, 100Å 10 µm, 100Å 10 mm ID x 5 cm TP TP mm ID x 10 cm TP TP mm ID x 15 cm TP TP mm ID x 25 cm TP TP mm ID x 5 cm TP TP mm ID x 10 cm TP TP mm ID x 15 cm TP TP mm ID x 25 cm TP TP mm ID x 5 cm TP TP mm ID x 10 cm TP TP mm ID x 15 cm TP TP mm ID x 25 cm TP TP Kromasil C 8 Column Size 7 µm, 100Å 10 µm, 100Å 10 mm ID x 5 cm TP TP mm ID x 10 cm TP TP mm ID x 15 cm TP TP mm ID x 25 cm TP TP mm ID x 5 cm TP TP mm ID x 10 cm TP TP mm ID x 15 cm TP TP mm ID x 25 cm TP TP mm ID x 5 cm TP TP mm ID x 10 cm TP TP mm ID x 15 cm TP TP mm ID x 25 cm TP TP

25 Value-Line Threaded Chromatography Columns Kromasil C 4 Column Size 7 µm, 100Å 10 µm, 100Å 10 mm ID x 5 cm TP TP mm ID x 10 cm TP TP mm ID x 15 cm TP TP mm ID x 25 cm TP TP mm ID x 5 cm TP TP mm ID x 10 cm TP TP mm ID x 15 cm TP TP mm ID x 25 cm TP TP mm ID x 5 cm TP TP mm ID x 10 cm TP TP mm ID x 15 cm TP TP mm ID x 25 cm TP TP Kromasil Silica Column Size 7 µm, 100Å 10 µm, 100Å 10 mm ID x 5 cm TPSI TPSI mm ID x 10 cm TPSI TPSI mm ID x 15 cm TPSI TPSI mm ID x 25 cm TPSI TPSI mm ID x 5 cm TPSI TPSI mm ID x 10 cm TPSI TPSI mm ID x 15 cm TPSI TPSI mm ID x 25 cm TPSI TPSI mm ID x 5 cm TPSI TPSI mm ID x 10 cm TPSI TPSI mm ID x 15 cm TPSI TPSI mm ID x 25 cm TPSI TPSI Daiso C18 Column Size 10 µm, 120Å 15 µm, 120Å 10 mm ID x 5 cm TP TP mm ID x 10 cm TP TP mm ID x 15 cm TP TP mm ID x 25 cm TP TP mm ID x 5 cm TP TP mm ID x 10 cm TP TP mm ID x 15 cm TP TP mm ID x 25 cm TP TP mm ID x 5 cm TP TP mm ID x 10 cm TP TP mm ID x 15 cm TP TP mm ID x 25 cm TP TP

26 Value-Line Threaded Chromatography Columns Daiso C 8 Column Size 10 µm, 120Å 15 µm, 120Å 10 mm ID x 5 cm TP TP mm ID x 10 cm TP TP mm ID x 15 cm TP TP mm ID x 25 cm TP TP mm ID x 5 cm TP TP mm ID x 10 cm TP TP mm ID x 15 cm TP TP mm ID x 25 cm TP TP mm ID x 5 cm TP TP mm ID x 10 cm TP TP mm ID x 15 cm TP TP mm ID x 25 cm TP TP Daiso C 4 Column Size 10 µm, 120Å 15 µm, 120Å 10 mm ID x 5 cm TP TP mm ID x 10 cm TP TP mm ID x 15 cm TP TP mm ID x 25 cm TP TP mm ID x 5 cm TP TP mm ID x 10 cm TP TP mm ID x 15 cm TP TP mm ID x 25 cm TP TP mm ID x 5 cm TP TP mm ID x 10 cm TP TP mm ID x 15 cm TP TP mm ID x 25 cm TP TP Daiso Silica Column Size 10 µm, 120Å 15 µm, 120Å 10 mm ID x 5 cm TPSI TPSI mm ID x 10 cm TPSI TPSI mm ID x 15 cm TPSI TPSI mm ID x 25 cm TPSI TPSI mm ID x 5 cm TPSI TPSI mm ID x 10 cm TPSI TPSI mm ID x 15 cm TPSI TPSI mm ID x 25 cm TPSI TPSI mm ID x 5 cm TPSI TPSI mm ID x 10 cm TPSI TPSI mm ID x 15 cm TPSI TPSI mm ID x 25 cm TPSI TPSI

27 Application-Specific Prepacked Columns MODcol has historically been known for combining media, packing procedures and hardware into products with extraordinary Prep-LC performance. We are now delivering this optimized package as application-specific prepacked columns. Chromatographers who need products suitable for demanding applications will appreciate the convenience of these products. We have eliminated the tedious task of selecting and optimizing the media and column, freeing time for other important tasks. The members of MODcol s application-specific family include: Columns for Chiral Separation Columns for Super-Critical-Fluid Chromatography Columns for Rapid and High-Throughput Separations Columns for Purification of Peptides and Proteins Please consult the following pages for a selection of these products. Name Retention Time Plates per Column Asymmetry(10.0%) Plates per Meter Toluene Reproducibility Vydac 300 Å spring columns. Name Retention Time Plates per Column Asymmetry(10.0%) Plates per Meter Toluene Kromasil 5um, C18 20 mm ID, 5 cm length column performance after 10,000 column volume of mobile phase. 23

28 Application-Specific Prepacked Columns Columns for Chiral Chromatography Kromasil s Chiral phases are covalently bonded to the same high performance spherical silica used throughout their product line. The covalent attachment of the bonded phase to the support material increases the chemical stability above the level achieved by other manufacturer's chiral materials. Kromasil chiral phases feature the same high mechanical strength, chemical purity, stability and well-defined pore structure found in other Kromasil materials. This media features a narrow particle size distribution for high efficiency, low-pressure drop and best total economy in chromatographic purification. Two phases have been developed to complement each other in selectivity and are offered in a 10 µm particle size with 100 Å pores. These high performance medias are offered in the flanged column product line. A test kit containing two 4.6mm x 250mm analytical columns (one each of the DMB and TBB phases) is also available as part number A00000-MDK001. Two phases have been developed to complement each other in selectivity: Kromasil CHI-DMB: The chiral monomer is 0,0'-bis (3,5-dimethylbenzoyl)- N,N'-diallyl-L-tartar diamide. Kromasil CHI-TBB: The chiral monomer is 0,0'-bis (4-tert-butylbenzoyl)- N,N'-diallyl-L-tartar diamide. Thermostatted spring column with integrel water jacket for chiral application. An application guide with examples of chiral separations performed on Kromasil materials and technical information can be found on the Kromasil web site at: Prepacked Flanged Columns DMB phase TBB phase Description 10 µm 100Å 10 µm 100Å 12 mm ID x 25 cm FPCH FPCH mm ID x 25 cm FPCH FPCH mm ID x 25 cm FPCH FPCH mm ID x 25 cm FPCH FPCH mm ID x 25 cm FPCH FPCH Prepacked Spring Columns DMB phase TBB phase Description 10 µm 100Å 10 µm 100Å 25 mm ID x 10 cm PSCH PSCH mm ID x 15 cm PSCH PSCH mm ID x 25 cm PSCH PSCH mm ID x 10 cm PSCH PSCH mm ID x 15 cm PSCH PSCH mm ID x 25 cm PSCH PSCH Other packed column sizes available upon request. 24

29 Application-Specific Prepacked Columns Columns for Super-Critical-Fluid Chromatography Super-Critical-Fluid Chromatography (SFC) is a rapidly expanding field of chromatography that uses a high-pressure liquid-like mobile phase, typically consisting of CO 2 and a modifier. MODcol is leading the industry with high performance columns for SFC applications that use specially designed highpressure seals to permit reliable use at the elevated pressures and temperatures commonly encountered. MODcol offers Kromasil and Daiso media in its SFC columns. Other column lengths and bonded phases are available on request. Silica Flanged Columns Description 12x25 column 7µm 100Å Kromasil Silica 18x25 column 7µm 100Å Kromasil Silica 25x25 column 7µm 100Å Kromasil Silica 50x25 column 7µm 100Å Kromasil Silica 12x25 column 10µm 100Å Kromasil Silica 18x25 column 10µm 100Å Kromasil Silica 25x25 column 10µm 100Å Kromasil Silica 50x25 column 10µm 100Å Kromasil Silica 12x25 column 10µm 120Å Daiso Silica 18x25 column 10µm 120Å Daiso Silica 25x25 column 10µm 120Å Daiso Silica 50x25 column 10µm 120Å Daiso Silica C18 Flanged Columns Description 12x25 column 7µm 100Å Kromasil C18 18x25 column 7µm 100Å Kromasil C18 25x25 column 7µm 100Å Kromasil C18 50x25 column 7µm 100Å Kromasil C18 12x25 column 10µm 100Å Kromasil C18 18x25 column 10µm 100Å Kromasil C18 25x25 column 10µm 100Å Kromasil C18 50x25 column 10µm 100Å Kromasil C18 12x25 column 10µm 120Å Daiso C18 18x25 column 10µm 120Å Daiso C18 25x25 column 10µm 120Å Daiso C18 50x25 column 10µm 120Å Daiso C18 Part Number XPSI XPSI XPSI XPSI XPSI XPSI XPSI XPSI XPSI XPSI XPSI XPSI Part Number XP XP XP XP XP XP XP XP XP XP XP XP The SFC column includes a special groove and O-ring design for a high-pressure seal. 25

30 Application-Specific Prepacked Columns Columns for Rapid and High-Throughput Separations MODcol delivers the highest quality columns for rapid LC applications by combining 20 years of know-how and expertise in column hardware, packing procedures and media to create a product line that is specifically designed for this demanding application. A new proprietary packing process has been developed that delivers outstanding performance with short columns. MODcol is offering both flanged and threaded columns in its high-throughput separations product line. To improve the sample throughput, a common solution is to increase the flow of the mobile phase. High flow produces very high shear forces within the bed. Shear-driven media degradation generates fine debris that clogs the outlet frit and increases backpressure, eventually making the column unusable. Media having high mechanical strength such as Kromasil and Daiso resist shear-driven breakdown and increases the use life of the column when compared to columns packed with less robust media. Daiso 5µm C18 performance in a 30mm ID x 5cm length column. C18 Flanged Columns Kromasil Daiso Description 5 µm 100Å 5 µm 120Å 18x05 column FP FP x10 column FP FP x15 column FP FP x25 column FP FP x05 column FP FP x10 column FP FP x15 column FP FP x25 column FP FP x05 column FP FP x10 column FP FP x15 column FP FP x25 column FP FP C 8 Flanged Columns Kromasil Daiso Description 5 µm 100Å 5 µm 120Å 18x05 column FP FP x10 column FP FP x15 column FP FP x25 column FP FP x05 column FP FP x10 column FP FP x15 column FP FP x25 column FP FP x05 column FP FP x10 column FP FP x15 column FP FP x25 column FP FP

31 Application-Specific Prepacked Columns C 4 Flanged Columns Kromasil Daiso Description 5 µm 100Å 5 µm 120Å 18x05 column FP FP x10 column FP FP x15 column FP FP x25 column FP FP x05 column FP FP x10 column FP FP x15 column FP FP x25 column FP FP x05 column FP FP x10 column FP FP x15 column FP FP x25 column FP FP Silica Flanged Columns Kromasil Daiso Description 5 µm 100Å 5 µm 120Å 18x05 column FPSI FPSI x10 column FPSI FPSI x15 column FPSI FPSI x25 column FPSI FPSI x05 column FPSI FPSI x10 column FPSI FPSI x15 column FPSI FPSI x25 column FPSI FPSI x05 column FPSI FPSI x10 column FPSI FPSI x15 column FPSI FPSI x25 column FPSI FPSI C18 Threaded Columns Kromasil Daiso Description 5 µm 100Å 5 µm 120Å 20x05 column TP TP x10 column TP TP x15 column TP TP x25 column TP TP x05 column TP TP x10 column TP TP x15 column TP TP x25 column TP TP

32 Application-Specific Prepacked Columns C 8 Threaded Columns Kromasil Daiso Description 5 µm 100Å 5 µm 120Å 20x05 column TP TP x10 column TP TP x15 column TP TP x25 column TP TP x05 column TP TP x10 column TP TP x15 column TP TP x25 column TP TP C 4 Threaded Columns Kromasil Daiso Description 5 µm 100Å 5 µm 120Å 20x05 column TP TP x10 column TP TP x15 column TP TP x25 column TP TP x05 column TP TP x10 column TP TP x15 column TP TP x25 column TP TP Silica Threaded Columns Kromasil Daiso Description 5 µm 100Å 5 µm 120Å 20x05 column TPSI TPSI x10 column TPSI TPSI x15 column TPSI TPSI x25 column TPSI TPSI x05 column TPSI TPSI x10 column TPSI TPSI x15 column TPSI TPSI x25 column TPSI TPSI

33 Application-Specific Prepacked Columns Columns for Purification of Peptides and Proteins The separation of proteins and polypeptides has become one of the largest applications in semi-preparative and preparative HPLC. With almost 20 years of experience, MODcol has created a complete solution for the protein chemist wanting to quickly and effectively purify proteins and peptides. MODcol has selected two media for this demanding application: the Vydac 300 Å and the Daiso 300 Å, both exhibiting very good separations and complementary selectivity. The selection of 300 Å pore is due to the fact that larger polypeptides must enter the pore interior to interact with the majority of the stationary phase. This allows separations that are not possible when separating large polypeptides on media having 100 Å pores. At the same time wide pore silica are well-suited for the separation of small peptides. MODcol offers flanged and Spring Columns in their product line for purification of peptides and proteins. Spring Columns provide the highest performance and enhanced column lifetime and represent the ultimate in separation technology available today. Name Retention Plates per Asymmetry Plates per Time Column (10.0%) Meter Uracil Phenol Toluene Napthalene Typical performance of Daiso 300 C18 10 µm in a preparative column. C18 Columns Daiso Vydac Description 10 µm 300Å µm 300Å 12x25 flanged column FP FP x25 threaded column TP TP x25 flanged column FP FP x25 spring column* PS PS x25 flanged column FP FP x25 spring column* PS PS Daiso Vydac Description 15 µm 300Å µm 300Å 12x25 flanged column FP FP x25 threaded column TP TP x25 flanged column FP FP x25 spring column* PS PS x25 flanged column FP FP x25 spring column* PS PS * Prepacked Spring Columns are not user-serviceable and must be returned to MODcol for repacking. 29

34 Application-Specific Prepacked Columns C 8 Columns Daiso Vydac Description 10 µm 300Å µm 300Å 12x25 flanged column FP FP x25 threaded column TP TP x25 flanged column FP FP x25 spring column* PS PS x25 flanged column FP FP x25 spring column* PS PS Daiso Vydac Description 15 µm 300Å µm 300Å 12x25 flanged column FP FP x25 threaded column TP TP x25 flanged column FP FP x25 spring column* PS PS x25 flanged column FP FP x25 spring column* PS PS C 4 Columns Daiso Vydac Description 10 µm 300Å µm 300Å 12x25 flanged column FP FP x25 threaded column TP TP x25 flanged column FP FP x25 spring column* PS PS x25 flanged column FP FP x25 spring column* PS PS Daiso Vydac Description 15 µm 300Å µm 300Å 12x25 flanged column FP FP x25 threaded column TP TP x25 flanged column FP FP x25 spring column* PS PS x25 flanged column FP FP x25 spring column* PS PS * Prepacked Spring Columns are not user-serviceable and must be returned to MODcol for repacking. 30

35 MultiPacker Instruments Series 2002 MultiPacker Instruments MultiPacker instruments are high-performance systems designed to pack MODcol Spring Columns. Their ease of operation and low cycle time provide a self-contained and versatile column packing solution. Spring Columns offer outstanding convenience and flexibility when used with the MultiPacker Instrument. The ability to continuously vary bed length up to 30cm and the advantages of its spring-driven axial compression mechanism provide a high performance, adaptive solution for both the product development and the demanding process chromatography arenas. Furthermore, compared to traditional column packing processes, MODcol Spring Columns require significantly less media per packing because the bed is formed from the entire quantity of media that is introduced into the column without wastage. More columns can be packed from the same batch of media, reducing concerns regarding lot-to-lot variations. Also, by reducing media requirements, the cost of the MultiPacker may be recouped in as little as a few months time. Unlike other axial compression systems, the MODcol Spring Column is selfcontained. Once packed, the Spring Column is independent of the MultiPacker Instrument. Subsequently, one MultiPacker can be used to maintain an entire fleet of Spring Columns. For the first time, the chromatographer has the ability to pack columns on demand in order to quickly respond to in-house column needs. Series 2002 Multipacker Instruments. The MultiPacker provides several unique advantages to the chromatographer. You will find peace of mind by being independent from column packers and manufacturers. In addition, you have the ability to pack columns when and where they are needed. Money is saved with every column packed because less media is required. You have control of bed length and column volume and columns can be unpacked and repacked in a matter of minutes. Configure the Spring Column to use the optional axial compression subsystem when you wish to extend column lifetime and eliminate voids or use the MultiPacker to pack spring columns without the axial compression subsystem in order to maintain a constant bed volume. Because axial compression systems are able to pack columns at a lower pressure the performance of less robust or economical media is superior when the MultiPacker is used. Less mechanical damage is imparted to media when packed using the MultiPacker making it a perfect match to delicate media that is not bonded to silica. Unlike other column packing instruments that use an awkward hand pump and can require hours for column packing, from beginning to end, Spring Column packing takes only minutes and operates with the push of a button. The MultiPacker uses modular construction techniques that make it lightweight and easily assembled or disassembled. Many key components use advanced aluminum casting techniques that reduce manufacturing costs and provide high strength. The MultiPacker Series 2002 instruments are easy-to-use packing stations specifically designed to be used with the MODcol Spring Columns. Two separate instruments are available for use with the 50mm ID Spring columns and the 25mm ID Spring Columns. MultiPacker Series 2002 instrument for 25mm ID Spring Columns Part Number: IM IM1 MultiPacker Series 2002 instrument for 50mm ID Spring Columns Part Number: IM IM1 MultiPacker Series 2002 Two-Instrument Bundle One MultiPacker for 25mm ID Spring Columns and one MultiPacker for 50mm ID Spring Columns Part Number: IM Please consult the following catalog section for a complete list of Spring Columns and Spring Column parts that may be used with the MultiPacker instruments. 31

36 Axial Compression Spring Columns About Spring Columns The MODcol Spring Columns described in this section are designed to be used in conjunction with a MultiPacker instrument. Please see the previous section for information on the MultiPacker product line. Spring Columns can also be order prepacked and ready-to-use, however prepacked Spring Columns are not user-serviceable and must be returned to MODcol for repacking. Please consult pages 7-30 for prepacked Spring Column products that are appropriate for your application. How to Order Spring Columns for Use with the MultiPacker Instrument MODcol Spring Columns and parts can be ordered with a variety of configurations and options. The 25mm ID and 50mm ID versions are offered in different lengths to suit your needs. Each complete Spring Column consists of a series of parts that are ordered separately: 1. A Base Spring Column Parts Kit (including column body, all seals, locking mechanism guide tube, endplate and assembly hardware. 70cm L Spring Column Parts Kit includes a set of packing spacers). Choose a 25mm or 50mm inner diameter, a 40cm or 70cm length, and a waterjacketed or non-water-jacketed configuration for the column body. 2. A ready-to-use piston assembly (consisting of the piston body, piston frit and Teflon sleeve). The piston assembly can be ordered with a 1 µm or 5 µm piston frit porosity. 3. A Spring Kit (choose the appropriate pressure range that will suit your needs). 4. An outlet frit (choose a 1 µm or 5 µm frit porosity). 5. Tubing and connectors (choose tubing with an outer diameter and bore that suits your needs). MODcol Axial Compression Spring Column components. MODcol Axial Compression Spring Column components. The guide on the following pages will help you select the combination of options that is appropriate for your needs. For further assistance, call your sales rep or the MODcol headquarters directly at the number shown at the bottom of the page. Example Purchase Order To order a 25mm ID Spring Column without water jacket to pack a 10cm bed length with 0.040" ID tubing and 5µm frits at up to 900psi packing pressure: Item Part number Description 1 SA Non-Water-Jacketed Spring Column, 25mm ID, 40cm L for bed lengths up to 15cm 2 AS A02 Assembled Piston for 25mm ID Spring Column, with 5µm frit 3 AF0000-C02503 Outlet Frit, 5µm porosity 4 AS A04 Spring Kit for 25mm ID Spring Columns, for packing pressures from 500 to 900psi 5 AS A07 1/16" OD 0.040" ID connecting tube and fittings 32

37 Axial Compression Spring Columns For 25mm ID Spring Columns, order one of each of the following items: 1. Spring Column Parts Kit - Choose an option 1.1. Non-Water-Jacketed Spring Column Parts Kit, 25mm ID, 40cm L. For bed lengths up to 15cm. Part Number SA Water-Jacketed Spring Column Parts Kit, 25mm ID, 40cm L. For bed lengths up to 15cm. Part Number SA2002-W Non-Water-Jacketed Spring Column Parts Kit, 25mm ID, 70cm L. For bed lengths up to 30cm. Part Number SA Water-Jacketed Spring Column Parts Kit, 25mm ID, 70cm L. For bed lengths up to 30cm. Part Number SA2002-W Piston Assembly - Choose an option 2.1. Piston Assembly for 25mm ID Spring Column with 1µm frit. Part Number AS A Piston Assembly for 25mm ID Spring Column with 5µm frit. Part Number AS A02 3. Outlet Frit - Choose an option 3.1. Outlet Frit with 1µm porosity. Part Number AF0000-C Outlet Frit with 5µm porosity. Part Number AF0000-C Spring Kit - Choose an option 4.1. Spring Kit for 25mm ID Spring Columns, for packing pressures from 1000 to 2000psi. Part Number AS A Spring Kit for 25mm ID Spring Columns, for packing pressures from 500 to 900psi. Part Number AS A Spring Kit for 25mm ID Spring Columns, for packing pressures up to 400psi. Part Number AS A05 5. Tubing and Connectors - Choose an option /16" OD, 0.020" ID connecting tube and fittings for use with 25mm ID Spring Column Part Number AS A /16" OD, 0.040" ID connecting tube and fittings for use with 25mm ID Spring Column Part Number AS A07 33

38 Axial Compression Spring Columns For 50mm ID Spring Columns, order one of each of the following items: 1. Spring Column Parts Kit - Choose an option 1.1. Non-Water-Jacketed Spring Column Parts Kit, 50mm ID, 40cm L. For bed lengths up to 15cm. Part Number SA Water-Jacketed Spring Column Parts Kit, 50mm ID, 40cm L. For bed lengths up to 15cm. Part Number SA2002-W Non-Water-Jacketed Spring Column Parts Kit, 50mm ID, 70cm L. For bed lengths up to 30cm. Part Number SA Water-Jacketed Spring Column Parts Kit, 50mm ID, 70cm L. For bed lengths up to 30cm. Part Number SA2002-W Piston Assembly - Choose an option 2.1. Piston Assembly for 25mm ID Spring Column with 1µm frit. Part Number AS A Piston Assembly for 25mm ID Spring Column with 5µm frit. Part Number AS A02 3. Outlet Frit - Choose an option 3.1. Outlet Frit with 1µm porosity. Part Number AF0000-B Outlet Frit with 5µm porosity. Part Number AF0000-B Spring Kit - Choose an option 4.1. Spring Kit for 50mm ID Spring Columns, for packing pressures from 1000 to 2000psi. Part Number AS A Spring Kit for 50mm ID Spring Columns, for packing pressures from 900 to 1500psi. Part Number AS A Spring Kit for 50mm ID Spring Columns, for packing pressures from 400 to 900psi. Part Number AS A Spring Kit for 50mm ID Spring Columns, for packing pressures up to 400psi. Part Number AS A06 5. Tubing and Connectors - Choose an option /16" OD, 0.040" ID connecting tube and fittings for use with 50mm ID Spring Column. Part Number AS A /8" OD, 0.060" ID connecting tube and fitting for use with 50mm ID Spring Column. Part Number AS A /8" OD, 0.085" ID connecting tube and fitting for use with 50mm ID Spring Column. Part Number AS A09 34

39 Axial Compression Spring Columns Replacement Parts Spring Columns are designed to be easily serviceable. Frit replacement is easily accomplished using the frit replacement kits. In addition to the parts that are listed above, a full array of replacement parts may be ordered from the list below. All of these parts can be reordered from MODcol at any time. For 25mm ID Spring Columns Part Number Description AF0000-C µm Outlet Frit for 25mm ID Spring Columns AF0000-C µm Outlet Frit for 25mm ID Spring Columns AH0000-R025A1 Oring Set for 25mm ID Spring Column Outlet AS A08 Replacement bolts, nuts, and washers for non-water-jacketed 25mm ID Spring Columns AS A11 Replacement bolts, nuts, and washers for water-jacketed 25mm ID Spring Columns AS Threaded Spacer for 25mm ID, 40cm L Spring Columns AS Threaded Spacer for 25mm ID, 70cm L Spring Columns AS Threaded Flange for 25mm ID Spring Columns AS Packing Spacer for 25mm ID Spring Columns AS Unpacking Spacer for 25mm ID, 40cm L Spring Columns AS Unpacking Spacer for 25mm ID, 70cm L Spring Columns AS Piston for 25mm ID Spring Columns AS A12 1µm Piston Frit replacement kit for 25mm ID Spring Columns AS A13 5µm Piston Frit replacement kit for 25mm ID Spring Columns For 50mm ID Spring Columns Part Number Description AF0000-D05001 Outlet Disperser Frit for 50mm ID Spring Columns AF0000-B µm Outlet Bed Support Frit for 50mm ID Spring Columns AF0000-B µm Outlet Bed Support Frit for 50mm ID Spring Columns AH0000-R050A1 Oring Set for 50mm ID Spring Column Outlet AS A10 Replacement bolts, nuts, and washers for non-water-jacketed 50mm ID Spring Columns AS A13 Replacement bolts, nuts, and washers for water-jacketed 50mm ID Spring Columns AS Threaded Spacer for 50mm ID, 40cm L Spring Columns AS Threaded Spacer for 50mm ID, 70cm L Spring Columns AS Threaded Flange for 50mm ID Spring Columns AS Packing Spacer for 50mm ID Spring Columns AS Unpacking Spacer for 50mm ID, 40cm L Spring Columns AS Unpacking Spacer for 50mm ID, 70cm L Spring Columns AS Piston for 50mm ID Spring Columns AS A14 1µm Piston Frit replacement kit for 50mm ID Spring Columns AS A15 5µm Piston Frit replacement kit for 50mm ID Spring Columns 35

40 Columns for Process-Scale Separations Large Scale Process Columns and Accessories MODcol builds columns with internal diameters of up to 600 mm (24 inches) on a custom-order basis for a variety of process and industrial applications. The design of MODcol process and industrial scale columns is based on the patented geometry used in its preparative and semi-preparative columns. In this way, production-scale separations can be transferred from MODcol s preparative family of flanged columns using the best available scalability. In addition, all MODcol columns greater than 4" I.D. are ASME or ISO certified to provide the maximum level of product safety and compliance with national and local regulatory boards and agencies. MODcol is able to build custom fixed bed and axial compression systems as permanent or mobile installations to suit the needs of a project or facility. We work with customers to provide individually tailored solutions. MODcol s expertise has been proven over and over again by repeat customers in both large and complex manufacturing and development projects. Take advantage of MODcol s advanced procedures, technology and column packing expertise when developing your complex separations. Common options available with custom large scale process columns include bed support frits or sieves with perforated support plates, an integrated pivoting mobile column stand, removable vibratory packing system for dry packing, sanitary or pipe fittings and other accessibility options. When soliciting quotations, please provide the desired pressure rating and the internal volume and/or the internal diameter and length of the column. 600mm ID industrial column (ASME certified). 36

41 Parts and Accessories Flanged Columns Modular in construction and manufactured to the highest standards, these patented stainless steel columns are known for durability and their special leak proof seal system. A uniform approach to the design of MODcol column components is used across MODcol s preparative, semi-preparative and industrial column product lines. These feature pure Teflon seals, passivated stainless steel frits, electro polished column bodies and endplates and Valco compression fittings. The result is a family of columns with exceptional scale-up performance. Empty Flanged Column Kit Each Empty Column Kit comes with the following: One Column Body One Pair of End Plates Frits, Orings and Tubing Connectors The pricing table below lists the lengths and diameter offered by MODcol in the standard product line. Other sizes are available on a custom basis. Please call your MODcol sales representative or dealer for additional information. Flanged column, exploded view. ID Column Length [cm] [mm] FA FA FA FA C C 18 FA FA FA FA C C 25 FA FA FA FA C C 30 FA FA FA FA C C 50 FA FA FA FA FA FA X FA FA FA FA FA X FA FA FA FA FA X = not available C = available as a custom order 37

42 Parts and Accessories Seals, Frits* and Assembly Hardware for Flanged Columns Description Assembly hardware for 12mm columns, set Seal rings for 12mm columns, pair Dual Density Frits for 12mm column, available in 1µm and 5µm porosities, pair Part Number AH0000-A01201 AH0000-R012A1 AF0000-B012Ax* Assembly hardware for 18mm columns, set Seal rings for 18mm columns, pair Dual Density Frits for 18mm column, available in 1µm and 5µm porosities, pair Assembly hardware for 25mm columns, set Seal rings for 25mm columns, pair Dual Density Frits for 25mm column, available in 1µm and 5µm porosities, pair Assembly hardware for 30mm columns, set Orings for 30mm columns, set Disperser frits for 30mm columns, pair Bed Support Frits for 30mm columns, available in 2µm, 5µm and 10µm porosities, pair Assembly hardware for 50mm columns, set Orings for 50mm columns, set Disperser frits for 50mm columns, pair Bed Support Frits for 50mm columns, available in 2µm, 5µm and 10µm porosities, pair Assembly hardware for 76mm columns, set Orings for 76mm columns, set Disperser frits for 76mm columns, pair Bed Support Frits for 76mm columns, available in 2µm, 5µm and 10µm porosities, pair Assembly hardware for 101mm columns, set Orings for 101mm columns, set Disperser frits for 101mm columns, pair Bed Support Frits for 101mm columns, available in 2µm, 5µm and 10µm porosities, pair AH0000-A01801 AH0000-R018A1 AF0000-B018Ax* AH0000-A02501 AH0000-R025A1 AF0000-B025Ax* AH0000-A03001 AH0000-R030A1 AF0000-D030A1 AF0000-B030Ax* AH0000-A05001 AH0000-R050A1 AF0000-D050A1 AF0000-B050Ax* AH0000-A07601 AH0000-R076A1 AF0000-D076A1 AF0000-B076Ax* AH0000-A10101 AH0000-R101A1 AF0000-D101A1 AF0000-B101Ax* Bed support frits for every type of media. * When specifying the part number for flanged column frits, please indicate the desired porosity by substituting the trailing x with 1 for 1µm, 2 for 2µm, 3 for 5µm and 4 for 10µm. 38