OIE Reference Laboratory Reports Activities
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1 OIE Reference Laboratory Reports Activities Activities in 2014 This report has been submitted : :47:51 Name of disease (or topic) for which you are a designated OIE Reference Laboratory: Salmonellosis Address of laboratory: New Haw, Addlestone Surrey KT15 3NB Weybridge UNITED KINGDOM Tel.: Fax: address: rob.davies@ahvla.gsi.gov.uk Website: Name (including Title) of Head of Laboratory (Responsible Official): Dr. Kath Webster Name (including Title and Position) of OIE Reference Expert: Rob Davies Which of the following defines your laboratory? Check all that apply: Governmental OIE Reference Laboratory Reports Activities,
2 ToR 1: To use, promote and disseminate diagnostic methods validated according to OIE Standards 1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (t for quality control, proficiency testing or staff training) Diagnostic Test Indicated in OIE Manual (/) Total number of test performed last year Indirect diagnostic tests Nationally Internationally SAT (S. Gallinarum/Pullorum) yes 22 SAT (S. Abortusequi) yes 75 SAT (S. Dublin) yes 66 SAT (S. Typhimurium) yes 16 Direct diagnostic tests Nationally Internationally Serotyping yes Phagetyping yes Antimicrobial susceptibility testing yes Tests for live Salmonella vaccines yes Salmonella culture yes 9000 ToR 2: To develop reference material in accordance with OIE requirements, and implement and promote the application of OIE Standards. To store and distribute to national laboratories biological reference products and any other reagents used in the diagnosis and control of the designated pathogens or disease. 2. Did your laboratory produce or supply imported standard reference reagents officially recognised by the OIE? 3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries? 2 OIE Reference Laboratory Reports Activities, 2014
3 Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg). of recipient OIE Member Countries Region of recipients Salmonella typing sera serotyping 31,526 ml 21,987 ml 600 ml commercially sensitive information America s Asia and Pacific Middle East S. Pullorum control serum SAT ml 2.25 ml 8 ml commercially sensitive information America s Asia and Pacific Middle East 4. Did your laboratory produce vaccines? 5. Did your laboratory supply vaccines to OIE Member Countries? ToR 3: To develop, standardise and validate, according to OIE Standards, new procedures for diagnosis and control of the designated pathogens or diseases 6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease? 7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientific and technical advice on disease control measures to OIE Member Countries 8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? OIE Reference Laboratory Reports Activities,
4 9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Name of the OIE Member Country receiving a technical consultancy Purpose How the advice was provided ALBANIA Technical advice on implementation of laboratory methods and the Salmonella National Control Programmes TAIEX Mission to ALbania; in situ assistance SERBIA Conference presentation and advisory visits to chicken breeding farms In situ assistance CHILE Advice on detection of S. Gallinarum/Pullorum Remote assistance IRELAND Sequencing of unusual isolates Testing of samples FINLAND Advice on S. Gallinarum/Pullorum serology Remote assistance GERMANY Advice on treatment/vaccination of chicken breeding flocks Remote assistance SAUDI ARABIA Advice on possible Salmonella-related illness in chickens Remote assistance UNITED ARAB EMIRATES Advice on serotyping/serogrouping Remote assistance FRANCE Advice on Screening of pigs for Salmonella Remote assistance BELGIUM Advice on Salmonella in pigs Remote assistance ToR 5: To carry out and/or coordinate scientific and technical studies in collaboration with other laboratories, centres or organisations 10. Did your laboratory participate in international scientific studies in collaboration with OIE Member Countries other than the own? 4 OIE Reference Laboratory Reports Activities, 2014
5 Title of the study Duration Purpose of the study Partners (Institutions) Salmonella in pigs 1 year Knowledge exchange on Salmonella in pigs various Molecular serotyping study 2 years Evaluation of molecular methods for serotyping Salmonella various Salmonella vaccine trial 2 years Field study; Salmonella in pigs IDT Biologica, BPEX DISCONTOOLS 3 years Collection and ranking of disease control and infection various S. Infantis study 1 year Molecular analysis and comparison of S. Infantis strains MTA Centre for Agricultural Research ToR 6: To collect, process, analyse, publish and disseminate epizootiological data relevant to the designated pathogens or diseases 11. Did your Laboratory collect epizootiological data relevant to international disease control? 12. Did your laboratory disseminate epizootiological data that had been processed and analysed? 13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category) a) Articles published in peer-reviewed journals: ARNOLD, M.E., GOSLING, R.J., LA RAGIONE, R.M., DAVIES, R.H. & FRANCESCA MARTELLI, F.M. (2014): Estimation of the impact of vaccination on faecal shedding and organ and egg contamination for Salmonella Enteritidis, Salmonella Typhiumurium and monophasic Salmonella Typhimurium, Avian Pathology, DOI: / ARNOLD, M.E., GOSLING, R.J., MARTELLI, F ; MUELLER-DOBLIES, D. & DAVIES R.H (2014). Evaluation of the sensitivity of faecal sampling for detection ofmonophasic Salmonella Typhimurium and other Salmonella in cattle and pigs. Epidemiol. Infect., Page 1 of 11. Cambridge University Press 2014 doi: /s ARNOLD M. E., MARTELLI, F., MCLAREN, I..& DAVIES R.H (2014). Estimation of the sensitivity of environmental sampling for detection of Salmonella in commercial layer flocks post-introduction of national control programmes. Epidemiol. Infect., 142, GOSLING, R.J., MARTELLI, F., WINTRIP, A., SAYERS, A.R., K. WHEELER, K. & DAVIES, R.H. (2014) Assessment of producers response to Salmonella biosecurity issues and uptake of advice on laying hen farms in England and Wales. British Poultry Science, 55 (5) DOI: / GOSLING, R.J., MARTELLI, F., SAYERS, R., LARKIN, L., DAVIES, R.H., (2014). A review of the official sampling of flocks of laying hens in the Salmonella National Control Programme in Great Britain. Br. Poult. Sci. 55, HORIGAN, V., DAVIES, R. H., KELLY, L. A., MEAD, G. C., IRVINE, R. M., & SIMONS, R. R. L. (2014). A qualitative risk assessment of the microbiological risks to consumers from the production and consumption of uneviscerated and eviscerated small game birds in the UK. Food Control, 45, OIE Reference Laboratory Reports Activities,
6 7. MARIER, E. A., SNOW, L. C., FLOYD, T., MCLAREN, I. M., BIANCHINI, J., COOK, A. J. C., & DAVIES, R. H. (2014). Abattoir based survey of Salmonella in finishing pigs in the United Kingdom Preventive Veterinary Medicine - on line ahead of publication. 8. MARTELLI, F., GOSLING, R., MCLAREN, I., WALES, A., & DAVIES, R. (2014). Development and testing of external quality assessment samples for Salmonella detection in poultry samples. Letters in applied microbiology, 59(4), MARTELLI, F., GOSLING, R., KENNEDY, E., RABIE, A., REEVES, H., CLIFTON-HADLEY, F., DAVIES, R., LA RAGIONE, R. (2014) Characterization of the invasiveness of monophasic and aphasic Salmonella Typhimurium strains in 1- day-old and point-of-lay chickens. Avian Pathol.43 (3): doi: / MUELLER-DOBLIES, D., CARRIQUE-MAS, J. and DAVIES, R. (2014). A study of the dynamics of Salmonella infection in turkey breeding, rearing and finishing houses with special reference to elimination, persistence and introduction of Salmonella. Avian Pathology 43 (2), THRELFALL, E.J., WAIN, J., PETERS, T., LANE, C., DE PINNA, E., LITTLE, C.L., WALES, A.D. & DAVIES, R.H. (2014). Egg-borne infections of humans with Salmonella: not only an Enteritidis problem. World's Poultry Science Journal. 70, b) International conferences: 5 GOSLING, R.J., MAWHINNEY, I., MUELLER-DOBLIES, D. The most appropriate sample type to detect Salmonella in a cohort of Salmonella positive duck flocks. 24th ECCMID, Barcelona, Spain, May 10-13, 2014 HORTON, R.A., CARD, R., RANDALL, L.P., TEALE, C. Differentiation of epidemic and endemic strains of Salmonella enterica serovar Newport by PCR detection of a truncated bapa chromosomal gene. 24th ECCMID, Barcelona, Spain, May 10-13, 2014 LAWES, J.R., KIDD, S.A., TEALE, C.J and DAVIES, R.H. Trends in Regulated Salmonella serovars in GB Livestock: Society for Veterinary Epidemiology and Preventive Medicine conference, Dublin, Ireland, March 26-28, 2014 MUELLER-DOBLIES, D., HORTON, R., DAVIES, R.H. Threat awareness and mitigation strategies for potential epidemic Salmonella in the United Kingdom. IMED 2014, Vienna, Austria, October 31-vember 3, 2014 RABIE, A., GOSLING, R.J., MARTELLI, F., DAVIES R.H. A study of the efficacy of products used for washing duck eggs. German Symposium on Zoonoses Research and 7th International Conference on Emerging Zoonoses, Berlin, Germany, October 16-17, 2014 c) National conferences: 2 CHENEY, T. Salmonella, Toxoplasma, Hepatitis E virus, Yersinia, PRRSv, Antimicrobial Resistance in Campylobacter coli & ESBL E. coli in UK pigs at slaughter. East of England Zoonoses Interdisciplinary Group Seminar, March 27, 2014 CHENEY, T. Salmonella, Toxoplasma, Hepatitis E virus, Yersinia, PRRSv, Antimicrobial Resistance in Campylobacter coli & ESBL E. coli in UK pigs at slaughter. ACMSF meeting, London, UK, 26 June 2014 d) Other: (Provide website address or link to appropriate information) 6 The battle against Salmonella. By: Mueller-Doblies, D. World Poultry, Volume: 30 Issue: 7 Pages: Published: 2014 ANON (2014) EFSA BIOHAZ Panel (EFSA Panel on Biological Hazards), Scientific Opinion on the evaluation of molecular typing methods for major food-borne microbiological hazards and their use for attribution modelling, outbreak investigation and scanning surveillance: Part 2 (surveillance and data management activities). EFSA Journal 2014;12(7):3784, 46 pp. doi: /j.efsa ANON (2014) EFSA BIOHAZ Panel (EFSA Panel on Biological Hazards), Scientific Opinion on the public health risks of table eggs due to deterioration and development of pathogens. EFSA Journal 2014;12(7):3782, 147 pp. doi: /j.efsa OIE Reference Laboratory Reports Activities, 2014
7 Journal: Pig World (August 2014) A dynamic approach to reducing high salmonella prevalence. By R. Smith and H. Clarke. Journal: Pig World (Sept 2014) - Trial underway to get salmonella vaccine licensed for UK. By R. Davies. Journal: Pig World (Oct 2014) - Finisher housing cleaning and disinfecting are central to salmonella control. By R. Davies and G. Crayford. ToR 7: To provide scientific and technical training for personnel from OIE Member Countries To recommend the prescribed and alternative tests or vaccines as OIE Standards 14. Did your laboratory provide scientific and technical training to laboratory personnel from other OIE Member Countries? a) Technical visits: b) Seminars: 1 c) Hands-on training courses: d) Internships (>1 month): 2 Type of technical training provided (a, b, c or d) Country of origin of the expert(s) provided with training. participants from the corresponding country b Ethiopia 5 d Spain 2 ToR 8: To maintain a system of quality assurance, biosafety and biosecurity relevant for the pathogen and the disease concerned 15. Does your laboratory have a Quality Management System certified according to an International Standard? Quality management system adopted ISO GLP ISO 9001 Certificate scan (PDF, JPG, PNG format) UKAS accreditation certificate.pdf GLP certificate APHA name change.pdf 9001 Certificate pdf 16. Is your laboratory accredited by an international accreditation body? OIE Reference Laboratory Reports Activities,
8 Test for which your laboratory is accredited Various Salmonella surveillance and diagnostic tests Various serological and AMR tests Various research methodologies Accreditation body UKAS UKAS Lloyds register 17. Does your laboratory maintain a biorisk management system for the pathogen and the disease concerned? (See Manual of Diagnostic Tests and Vaccines for Terrestrial Animals 2014, Chapter 1.1.3a) ToR 9: To organise and participate in scientific meetings on behalf of the OIE 18. Did your laboratory organise scientific meetings on behalf of the OIE? 19. Did your laboratory participate in scientific meetings on behalf of the OIE? Title of event Date (mm/yy) Location Role (speaker, presenting poster, short communications) Title of the work presented Ad-hoc working group on Salmonella control in pigs August 2014 Paris chair Terrestrial Code Ad-hoc working group on Salmonella control in Cattle December 2014 Paris chair Terrestrial Code ToR 10: To establish and maintain a network with other OIE Reference Laboratories designated for the same pathogen or disease and organise regular inter-laboratory proficiency testing to ensure comparability of results 20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease? 21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the 8 OIE Reference Laboratory Reports Activities, 2014
9 same pathogen or disease by organising or participating in proficiency tests? Purpose of the proficiency tests: 1 Role of your Reference Laboratory (organiser/ participant). participants Participating OIE Ref. Labs/ organising OIE Ref. Lab. Purpose of the proficiency tests: Assess Laboratory capability to conduct isolation and identification of Salmonella species to help maintain accreditation. Animal and Plant Health Agency Vetqas PT scheme PT0087 Salmonella in Animal Feed. Participant 21 UK Assess Laboratory capability to conduct isolation and identification of Salmonella species to help maintain accreditation. Animal and Plant Health Agency Vetqas PT scheme PT0088 Salmonella in Poultry Participant 131 UK, Canada Assess Laboratory capability to conduct isolation and identification of Salmonella species to help maintain accreditation. Animal and Plant Health Agency Vetqas PT scheme PT0090 Control of Salmonella in Poultry Order (run for Defra for UK labs only) Participant 25 UK 1 validation of a diagnostic protocol: specify the test; quality control of vaccines: specify the vaccine type, etc. 22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientific research projects for the diagnosis or control of the pathogen of interest? ToR 11: To organise inter-laboratory proficiency testing with laboratories other than OIE Reference Laboratories for the same pathogens and diseases to ensure equivalence of results 23. Did your laboratory organise or participate in inter-laboratory proficiency tests with laboratories other than OIE Reference Laboratories for the same disease? te: See Interlaboratory test comparisons in: Laboratory Proficiency Testing at: see point 1.3 OIE Reference Laboratory Reports Activities,
10 Purpose for inter-laboratory test comparisons 1. participating laboratories Region(s) of participating OIE Member Countries Assess Laboratory capability to conduct isolation and identification of Salmonella species to help maintain accreditation. Animal and Plant Health Agency Vetqas PT scheme PT0087 Salmonella in Animal Feed. 21 Assess Laboratory capability to conduct isolation and identification of Salmonella species to help maintain accreditation. Animal and Plant Health Agency Vetqas PT scheme PT0088 Salmonella in Poultry 131 Assess Laboratory capability to conduct isolation and identification of Salmonella species to help maintain accreditation. Animal and Plant Health Agency Vetqas PT scheme PT0090 Control of Salmonella in Poultry Order (run for Defra for UK labs only) 25 EURL Salmonella ringtrial for PFGE EQA-6 ringtrial for both PFGE and MLVA Salmonella detection, serotyping and phagetyping EU RL ringtrials 28 ToR 12: To place expert consultants at the disposal of the OIE 24. Did your laboratory place expert consultants at the disposal of the OIE? Kind of consultancy Location Subject (facultative) Commissioned literature review UK/Italy Control of Salmonella in food producing animals 25. Additional comments regarding your report: 10 OIE Reference Laboratory Reports Activities, 2014
11 This report was compiled by Doris Mueller-Doblies on behalf of Robert Davies. OIE Reference Laboratory Reports Activities,
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