Old Dialysis Technical Guy
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1 John A. Sweeny Old Dialysis Technical Guy 1
2 WHAT IS UREA? A compound formed in the liver by the process known as the urea cycle. Gram Molecular Weight = 60 Daltons Carbon - Oxygen - Nitrogen - Hydrogen - Principal end product of protein catabolism. Constitutes about half of the total urinary solids. Elevated level in blood = Azotemia BUN = Blood Urea Nitrogen = Amount of nitrogen in blood. Ratio: Nitrogen / Urea = 28/60 =
3 Protein Basics Proteins are large molecules made primarily from hundreds to thousands of amino acids. AMINO R ACID (NH 3+ ) CH (COO + ) There are 20 amino acids used to build human proteins. 9 are essential (You must eat them.) 11 are nonessential (You can make them.) 3
4 Protein Possibilities For a protein 100 amino acids long, there are possible combinations. 12,675,060,000,000,000,000,000,000,000,000, 000,000,000,000,000,000,000,000,000,000, 000,000,000,000,000,000,000,000,000,000, 000,000,000,000,000,000,000,000,000,000, 000,000,000 possibilities!! ( x ) 4
5 Dietary Protein Intake - DPI Normal DPI should be in the range of grams/kilogram of body weight/day. Effects of malnutrition begin to appear when the DPI is reduced to the gm/kg/day range. The type of protein consumed is important. t High-Biologic-Value Protein (Lean Meat, fish, and eggs) Low-Biologic-Value Protein (Breads and Cereals) HBV proteins contain the essential amino acids. Without HBV protein, muscle tissue is broken down for its amino acids. 5
6 Protein Content in Food 1 oz. Beef, Pork, Chicken = 8 grams 1 oz. Clams, Shrimp, Cod = 6 grams 1 oz. Cheese, Peanut Butter = 8 grams 1 cup cereal = 2 grams 1 Baked Potato = 5 grams 1/3 cup Spinach, Corn, Squash = 2 grams 1/2 cup Kidney Beans, Tomato = 2 grams 1/2 cup of fruit < 1 gram 6
7 Converting Protein to Urea In general, DPI will equal Protein Catabolic Rate (PCR) in the stable patient. The equation which relates protein intake to urea nitrogen generation rate (G) has been determined by monitoring diet and urea nitrogen in urine. 7
8 The Conversion Equation Protein to Urea Nitrogen PCR = 935G or G = (PCR ) / 9.35 PCR (grams/day) G (milligrams of urea nitrogen/minute) 8
9 BUN Generation Rate vs. Protein Catabolic Rate 9.50 (mg/minute e) BUN Gene eration Rate Protein Catabolic Rate (g/kg/day) 9
10 Introducing the Patient Name: Rusty Pipes Occupation: Plumbing Specialist Weight: kilograms (154.3 pounds) Fluid Volume: 40.6 liters (58% of his weight) Residual Renal Function: None DPI: Varies between g/kg/day Nephrologist: Dr. I. M. Nephron 10
11 BUN Build-upup in Rusty Rusty s s Volume = 40.6 liters = 406 deciliters If DPI = PCR = 84 g/day (1.2 g/kg/day x 70 kg), then: G = ( ) / 9.35 = 7.80 mg/min mg/min x 60 min/hr = 468 mg/hr. 468 mg/hr x 24 hr/day = 11,232 mg/day 11,232 mg/day / 406 dl = 27.7 mg/dl/day. 11
12 Rusty's PCR vs. Pre Treatment BUN 130 (mg/dl) Pre Treatm ment BUN days 3 days Rusty's PCR (grams/day) 12
13 Dialyzer BUN Clearance THE EMPIRICAL FORMULA FOR BLOOD CLEARANCE IS: C X A X A X V X Q B WHERE: C X = CLEARANCE OF SOLUTE X. (ml/min) A X = ARTERIAL CONCENTRATION OF X. (mg/dl) V X = VENOUS CONCENTRATION OF X. (mg/dl) Q B = BLOOD FLOWRATE (ml/min) 13
14 Urea Clearance in Blood and Dialysate Rusty s Dialyzer: ip4u ARTERIAL BLOOD 80 mg/dl K O A = 900 ml/min Q B = 300 ml/min Q = 600 ml/min DIALYSATE OUT Q D DIALYSATE OUT 35.0 mg/dl Q B x BUN B = Q D x BUN D DIALYSATE IN 0.0 mg/dl 300 x (80 10) = 600 x (35 0) 300 x 70 = 600 x 35 g 21,000 = 21,000 VENOUS BLOOD 10.0 mg/dl 14
15 Rusty s ip4u Clearance A BUN = 80 mg/dl V BUN = 10 mg/dl Q B = 300 ml/min C BUN = [(A BUN - V BUN )/A BUN ]xq B C BUN = [ (80-10) / 80 ] x 300 C BUN = 262 ml/min 15
16 Clearance vs. K O A K O A = Mass Transfer Coefficient for a Dialyzer K O A is measured in ml/min. K O A gives Clearance for Q B and Q D combinations. The range of K OA values is ml/min. The higher the K O A, the better the clearance. Once the K OA for a dialyzer is known, the clearance of that dialyzer can be determined for any combination of blood and dialysate flow rates. Of course, there s an equation 16
17 The K O A Equation KoA Q B Q B Q n D 1 C Q C X D X 1 QB Where: C X = Clearance of solute, X Q B = Blood flowrate Q D = Dialysate flowrate ln = Natural logarithm e =
18 BUN of Venous Blood based on Dialyzer KoA QB = 300 ml/min QD = 600 ml/min KoA Clearance Arterial Blood BUN Values (mg/dl) (ml/min) (ml/min)
19 Clearance vs. KoA arance (ml L/min) Cle Qb = 300 Qb = 400 Qb = 500 Qb = KoA (ml/min) 19
20 Rusty's BUN for the Week BUN in mg/d dl Patient Normal Time in hours 20
21 130.0 Rusty on Daily Home Hemodialysis BU UN (mg/dl) BUN (mg/dl) Variation Comparison Avg. BUN Std. Dev. Highest Lowest Range 3X/WK X/WK
22 Urea Reduction Formula (UFR = 0.0 ml/hr) C = C 0 e -Kt/V + G/K (1 - e -Kt/V ) C = Plasma BUN Concentration (mg/ml)* C 0 = Predialysis BUN Concentration (mg/ml)* K = Dialyzer Clearance (ml/min) t = time (minutes) V = Patient Volume (ml) G = Generation of urea (mg/min) * mg/ml equals mg/dl divided by
23 ) L /d g (m N U B Rusty's Mid-week Urea Reduction TIME (minutes) 23
24 Volume: Dialyzer vs. Rusty 80 mg/dl ip4u 1 deciliter Rusty 406 deciliters 80 mg/dl 10 mg/dl 24
25 Rusty s s Week on Dialysis URINE LUCK DIALYSIS CENTER TRX #1 TRX #2 TRX #3 Monday Wednesday d Fid Friday Pre BUN (mg/dl) Post BUN (mg/dl) URR 71.0% 70.4% 70.1% Kt/V BUN Removed (g) Urea Removed (g)
26 Rusty s Urea Loss BUN REMOVED = (BUN PRE - BUN POST ) X V PATIENT BUN PRE = 80.0 mg/dl BUN POST = 23.7 mg/dl Rusty s s Volume = 406 deciliters BUN REMOVED = ( ) X 406 = 22,858 mg = 22.9 grams UREA REMOVED = (60/28) X 22.9 grams = 49.0 grams 26
27 Urea down the Drain URINE LUCK DIALYSIS CENTER Number of stations: 20 Shifts per day: 3 Days of operation: Monday - Saturday Number of patients: 105 Avg. urea loss / R X : 52.2 grams (24.4 g BUN) Total R X per week: 295 Total drain urea: 15.4 kg/wk (33.9 lbs/wk) Equivalent urine: 1,660 liters (438 gallons) 27
28 Additional Urine Information Urine composition: 950 grams of water 9.3 grams Urea 1.87 grams Chloride 1.17 grams Sodium 0.75 grams Potassium 0.67 grams of Creatinine It s color comes from Urobilin from the breakdown of Hemoglobin Specific Gravity = g/cm 3 After eating Asparagus, urine will contain Asparagusic acid! 28
29 Patient's Blood Urea Nitrogen for the Week (Normal BUN in Yellow) 120 BUN in mg/dl Time in hours 29
30 Patient's Mid-week Treatment Urea Reduction BUN (mg g/dl) TIME (minutes) 30
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