AstaReal Astaxanthin for Sports Nutrition

Transcription

1 AstaReal Astaxanthin for Sports Nutrition

2 Agenda AstaReal Review Why AstaReal Astaxanthin for Performance Nutrition Astaxanthin Mechanism of Action for Muscle Health Human Clinical Trials with AstaReal Discussion & Next Steps

3 Who is AstaReal, Inc? A subsidiary of Fuji Chemical Industries Japanese Pharmaceutical Company since 1946 Astaxanthin Pioneer Launched AstaReal Brand in 1995 Built Natural Astaxanthin Market Astaxanthin Experts: unrivaled technical & marketing support Established Astaxanthin Safety & Quality Standards Established USP Monograph for Astaxanthin Quality Serving as USP Industry Reference Standard for Astaxanthin 3

4 AstaReal : The Astaxanthin Gold Standard Proprietary Manufacturing Process Unique closed indoor cultivation Filtered air Purified RO water Uninterrupted supply assurance Consistent Potency; >4.5% every batch High stability Made in the USA Science Based Nutrition Most Clinically Studied Astaxanthin Brand Worldwide Category Leader with R&D investment 4

5 AstaReal : The Most Studied Astaxanthin Brand Astaxanthin Pioneer with more than 150 published AstaReal studies since 1980 s and more than 60 published AstaReal human clinical studies 12 published AstaReal human studies 2 new AstaReal studies coming soon 4 published AstaReal human studies 16 published AstaReal human studies Computer Vision Syndrome astaxanthin data exclusive to AstaReal 13 published AstaReal human studies 6 published AstaReal human studies Investment in research & innovation Expanding health platforms

6 AstaReal Astaxanthin for Muscle Performance Helping active consumers meet health, performance and fitness goals

7 REfuel your Routine with AstaReal Astaxanthin 12 published AstaReal human studies 2 new AstaReal studies coming soon Suggested Muscle Function Structure/Function claims: AstaReal Astaxanthin reduces buildup of lactic acid1, 12 AstaReal Astaxanthin supports endurance and muscle performance 2,3 AstaReal Astaxanthin supports sustained energy and athletic performance 2, 3 AstaReal Astaxanthin helps improve power output 3 AstaReal Astaxanthin supports muscle recovery4, 5, 6 AstaReal Astaxanthin protect against exercise-induced muscle damage4, 5, 6 AstaReal Astaxanthin promotes good circulation; nutrition & oxygenation of muscles AstaReal Astaxanthin promotes mental clarity and focus10, 11 7, 8, 9

8 Astaxanthin: An Important Protective Nutrient

9 Astaxanthin: Nature s Most Powerful Antioxidant

10 Natural Astaxanthin: Best Coverage for Protection Vitamin C Astaxanthin Supports Membrane Structure small-angle x-ray diffraction measuring membrane density Beta Carotene Astaxanthin Vitamin E McNulty H. et al. Am J Cardiol 2008;101[suppl]:20D 29D) Nishida et al (2007). Carotenoid Science. Vol.11:16-20.

11 The Astaxanthin Advantage Why is Astaxanthin Nature s Most Powerful Antioxidant Astaxanthin is strongest natural antioxidant on the ORAC scale Astaxanthin is a pure antioxidant No conversion to Vitamin A Astaxanthin spans cell membranes Does not disrupt membrane structure Astaxanthin crosses the blood brain barrier Astaxanthin crosses the blood retinal barrier Astaxanthin has anti-inflammatory properties Beta-carotene Astaxanthin Vitamin C Vitamin E

12 Astaxanthin Improves Muscle Endurance Lactic Acid Cycle

13 Astaxanthin Neutralizes Free Radicals Exercise increases the production of reactive oxygen species (ROS) to a point that exceeds endogenous antioxidant defenses Increased metabolic rate of skeletal muscle = increased oxygen consumption Increased oxygen consumption = elevated ROS production in mitochondria ROS

14 Astaxanthin Protects Mitochondrial Membrane Non-Treated Group Astaxanthin-Treated Group Protective effect of astaxanthinon mitochondria membrane potential of SH-SY5Y cells, cells pretreated with 100 nm astaxanthin for 4h and subsequently treated with 10 μm DHA- OOH or 100 μm6-ohda for 1 h Liu et al (2009) Brain Research vol 1254;18-27 Mitochondrial membrane potential. Membrane potential images of HeLa cells cultured for 2 days in the presence or absence of 800 nmax. Wolf et al (2010) J of NutrBiochemvol21;

15 Astaxanthin Protects Muscle from Damage Astaxanthin limits the damage of heart muscle and leg muscle from oxidation Fat oxidation during exercise 0.02% astaxanthin for 3 weeks CONTROL EXERCISE CONTROL EXERCISE + AX Heart muscle Leg muscle Source: Aoi et al. (2003) Antioxidants & Redox Signalling, 5:(1)

16 AstaReal Astaxanthin Human Clinical Studies for Muscle Performance

17 AstaReal Helps Improve Muscle Performance 40 healthy paramedic students (ages 17-19) undergoing strength and endurance training - 20 treated with placebo - 20 treated with 4 mg/day AstaReal Astaxanthin AstaReal Astaxanthin Boosts Strength/Endurance Training Number of squats Placebo Astaxanthin Baseline 6 months Malmsten, et al., Carotenoid Science, 13, 20-22(2008).

18 AstaReal Helps Improve Power Output 14 competitive cyclists (ages 18-39) had their performance tested in a 20km timed trial. -7 cyclists treated with placebo -7 cyclists treated with 4mg/day AstaReal Astaxanthin 4 weeks Earnest,et al.,int J Sports Med., 32 (11): (2011).

19 AstaReal Helps Protect Muscles from Inflammation 40 trained soccer players (average 18 years old) - 19 treated with placebo - 21 treated with 4mg/day AstaReal Astaxanthin AstaReal Astaxanthin Blunts Systemic Inflammation placebo astaxanthin 1.98 CRP (mg/l) Baseline 90 days Baralic et al. Evidence-Based Complementary and Alt. Med. 2015(4): 1-9 (2015).

20 AstaReal Helps Protect Muscles from Damage 32 elite soccer players (average 18 years old) - 14 players were treated with placebo - 18 players were treated with 4mg/day AstaReal Astaxanthin Pre-exercise Post-exercise Pre-exercise Post-exercise Djordjevic et al., The Journal of sports medicine and physical fitness 52(4): (2012).

21 AstaReal Astaxanthin Reduces Lactic Acid 18 track athletes (average 20 years old) - 9 runners were treated with placebo - 9 runners were treated with 6mg/day AstaReal Astaxanthin Study Summary Randomized, double-blind placebo controlled study 16 track athletes took 6mg/daily for 4 weeks 1200 meters run Juntendo University, Japan Result Blood lactic acid level(mml) 14 Before Supplementation After Supplementation minutes 4-minutes 8-minutes 10-minutes Source: A0135 K. Sawaki, et al, J. Clinical Therapeutics & Medicines, 18(9), (2002).

22 AstaReal Helps Promote Mental Clarity & Focus Astaxanthin crosses the blood brain barrier 96 healthy adults (Average age 56) - 31 treated placebo - 29 treated 6mg/day - 29 treated with 12mg/day AstaReal Astaxanthin AstaReal Astaxanthin Fights Mental Fatigue Mean response times (ms) AstaReal Astaxanthin Promotes Faster Reaction Times (12mg/day) 6% Faster Baseline 12 weeks Choice reaction 8% Faster Baseline 12 weeks Spatial Attention Katagiri et al. J. Clin. Biochem. Nutr.. 51 (2): (2012). 39 healthy adults (20-64 years old) - 19 treated placebo -20 treated 12mg/day AstaReal Astaxanthin for 8 weeks. Hongo et al. Journal of Clinical Therapeutics & Medicines. 32 (7): (2016).

23 REviveyour Movement with AstaReal Astaxanthin

24 Joint Health Affects a Growing Number of People in the USA

25 Improving Joint Health Standard of Care Weight loss to reduce pressure on joints Exercise to support joints with strong muscles Nonsteroidal anti-inflammatory drugs (NSAIDs) - Linked to increased incidence of cardiovascular events Wear and tear Autoimmune Structures Supporting Healthy Joints Strong muscles support joint alignment and reduce pressure on joints Strong bones better weight distribution and support Good circulation nourish and oxygenate joints Healthy cartilage & tendons for shock absorption and fluid, painless motion

26 Weak Muscles Affect Joint Health Aging is associated with loss of muscle mass weight shifting joints out of alignment increased pressure on joints < 40 yrs > 65 yrs Pressure on joints may lead inflammation and ROS, which can degrade joint function over time Janssen I, Heymsfield SB, Ross R. J Am Geriatr Soc. 2002;50:

27 Astaxanthin as the Solution to Joint Health 1. Astaxanthin improves muscle performance, endurance, and recovery to build muscles able to support joint function. 2. Astaxanthin alleviates perceived muscle and joint pain. 3. Astaxanthin reduces inflammation that can damage joints. 4. Astaxanthin improves circulation, bringing nutrients and oxygen to promote the maintenance of healthy joints.

28 Healthy Aging Exercise Plus Astaxanthin improves mobility Don t go it alone! You + Astaxanthin Clinical evidence shows AstaReal astaxanthin together with exercise works better for building strength and endurance than exercise alone. + 1 mo mo Exercise Plus Astaxanthin Strength Endurance Contractions

29 Natural Astaxanthin Consumers Report Less Joint Pain In a survey of 146 consumers, 88% reported improvement in sore muscles and joints. Guerin M. et al TRENDS in Biotechnology Vol.21(5): participants experiencing carpal tunnel syndrome reported experiencing less pain after supplementing with 12 mg/day astaxanthin at 4 weeks and 8 weeks. Nir Y. et al BioAstin, a natural astaxanthin from micro-algae, helps relieve pain and improves performance in patients with carpal tunnel syndrome (CTS). Unpublished Report. 20 weight-trained males N= 10 astaxanthin N= 10 placebo Supplementing for 3 weeks Astaxanthin group experienced significantly less knee soreness for up to 48 hrsfollowing knee extension exercise compared to placebo (p<0.05) Fry, A.C Astaxanthin Clinical Trial for Delayed Onset Muscular Soreness. Unpublished Report.

30 Natural Astaxanthin Improves Grip Strength Muscles and Joints work together to allow good hand grip 33 subjects aged taking 4mg/day natural astaxanthin for 8 weeks saw improvement in grip strength (p<0.05). Spiller G.A. et al Unpublished GRIP STRENGTH MEASUREMENT (MMHG) Baseline 8 weeks

31 Inflammation and Joint Health Osteoarthritic joints exhibit elevated inflammation and production of Nitric Oxide (NO) Normal NO levels improve circulation (vasodilation) High NO levels are pro-inflammatory, may enhance chondrocyte death, and reduce cartilage matrix synthesis

32 Astaxanthin Reduces Inflammation NO is produced by inducible nitric oxide synthase (inos) in response to activation of nuclear factor (NF)-κB inflammation signaling pathway Normal iris-ciliary body LPS administration LPS + Astaxanthin ** p<0.01 NF-kB positive cells NF-kB p65 were immunostained with antibodies Green: normal nuclei Red: NF-kB p65 Experimental Eye Research 82 (2006)

33 DY Astaxanthin Improves Circulation Good circulation brings nutrients and oxygen to support joint health and cartilage matrix production Astaxanthin improves blood rheology (transit time) Blood Transit Time (Sec/100μL) Before After * # Single blind 20 healthy mid-age male subjects with a blood transit time of seconds 0 mg (placebo, n=10) 6 mg (n=10) astaxanthin per dayor placebo for 10 days AstaREAL administration shortened the blood transit time compared to start & placebo Placebo Treated *: p<0.01(vs start, FT-test) #:p<0.05(vs placebo, t-test) H. Miyawaki et al., 2005, Journal of Clinical Therapeutics & Medicines 21(4):421(33)-429(41).

34 REvive your Movement with AstaReal Astaxanthin - Concept Muscles and joints mediate everyday activities such as walking, gardening, sitting, and running. Joints are supported by a muscular framework and protected from the impact of movement by cartilage. Daily activities can put strain on joints over time, leading to inflammation, pain, and stiffness. Muscles also lose strength as we age contributing to strained and weakened joints. This strain is partly caused by a buildup of damaging free radicals that can arise from persistent inflammation. AstaReal Astaxanthin neutralizes free radicals, improving the overall health of joints. AstaReal Astaxanthin also supports muscle function, and together with exercise, natural astaxanthin can help support the muscular framework that contributes to strong and healthy joints. allowing you to keep moving and live a healthier life. Now formulated with 4mg of AstaReal Astaxanthin -to promotesustained muscle energy, protect against weakening joints, and increase muscle and joint performance.

35 Summary Natural Astaxanthin improves muscle health in aging populations, for better joint support Consumers of natural astaxanthin report experiencing less joint pain and improved grip strength Natural Astaxanthin help to reduce inflammation that can degrade joints over time and cause painful joints AstaReal Astaxanthin improves circulation to nourish joints

36 Not All Astaxanthin is the Same Exploring the Differences Between AstaReal Astaxanthin & Other Astaxanthin Suppliers

37 AstaReal : Unrivaled Cultivation Expertise AstaReal Fully Matured Red Phase Company B Incomplete Red Phase Red=Astaxanthin Green=Chlorophyll

38 AstaReal - The Most Studied Astaxanthin Brand Source Efficacy AstaReal Astaxanthin Haematococcus pluvialis CONFIDENTIAL Number of Published Clinical Trials Astaxanthin Brand Most Studied Worldwide Proven efficacy in 60+ human studies; over 150 studies with AstaReal X Leading Holder of Patents Related to Use of Astaxanthin in Humans X 12 approved patents as of 2016 Only Astaxanthin Brand Shown to Improve Eye Strain and Fatigue Most Associated with Computer Vision Syndrome Only Astaxanthin Brand Shown to Improve Muscle Strength in Aging Population X 30+ studies on eye health X 25+ studies on muscle health

39 AstaReal - The Safest Astaxanthin Brand WorldWide CONFIDENTIAL Safety AstaReal Astaxanthin GRAS FDA notified (2010) Leader in Establishing Astaxanthin Safety Health Canada Natural Product Number (NPN) NSF Certified Approved for pet supplements Free of Skin Irritating Pheophorbide Free of Prop65 Polyaromatic Hydrocarbons (PAH;carcinogens) X Sponsored over 40+ safety studies including a large number of clinical studies X The only astaxanthin brand with NPN from Health Canada X X The only astaxanthin brand approved as NASC preferred supplier X Testing confirmed no pheophorbide X

40 AstaReal - The Gold Standard in Quality Country of Origin Quality AstaReal Astaxanthin USA CONFIDENTIAL Established USP Monograph for Astaxanthin Quality Serving as USP Industry Reference Standard for Astaxanthin X X Consistent Supply Assurance Pharmaceutical manufacturing expertise of over 6 decades; 2 global manufacturing sites (Europe & USA); controlled indoor facilities with filtered air and purified RO water even for cleaning Consistent Potency Proprietary manufacturing process controlling potency; >4.5% astaxanthin every batch Astaxanthin Stability Bioavailability High stability; more stable in direct comparison to other competitive products Offers proprietary high bioavailability product form

41 Thank you for your Time! Questions?

42 AstaReal Human Clinical Studies for Muscle Performance August 2017

43 Muscle Performance Body of Evidence for AstaReal Astaxanthin Study Dosage Participants Method Result Claims 6mg/day Serum lactic acid concentration Astaxanthin treatment group had significantly less serum for 4 was measured 2min after a lactic acid (10mmol) 2min after a 1200m run than the control weeks 1200m run. group (14mmol) SawakiK. et al Malmsten C.L. et al Earnest C.P. et al mg/day for 6 months 4mg/day for 4 weeks 16 healthy adult distance runners on track and field team of a Juntendo university, Japan; average 20 years old 40 healthy paramedic students; ages adult competitive cyclists, male; ages Strength/endurance measured by maximum number of Smith machine 90 squats (similar to bar bell squats) with 42.5kg weight. 2h and 20km timed trial: Fasting: 10 hours to increase reliance on fat oxidation for energy Warm up: 50W (10min) Steady state pre-exhaustion ride: 2 hours. Rest: 5 min Timed Trial: cyclists instructed to ride 20km as fast as possible and average power output was measured every 5 km Astaxanthin group had a significant increase in total squats (up an average of 27; started at 49, increased to 76) after 6 months of training compared to control group (up an average of 9 squats; started at 46, increased to 55). Astaxanthin treated group showed average 20W increase (15%) in power output during 20km timed trial, compared to 0.5% power output increase in placebo. Astaxanthin treated group at average 2min (5%) improvement in time to complete 20km timed trial, compared to 0.8% improvement in time in placebo group AstaReal Astaxanthin reduces lactic acid AstaReal Astaxanthin improves muscle performance AstaReal Astaxanthin improves power output and athletic performance

44 Muscle Performance Body of Evidence for AstaReal Astaxanthin Study Dosage Participants Method Result Claims DjordjevicB. et al mg/day for 90 days 32 elite soccer players, male; average age, 18 Basal antioxidant enzyme activity, oxidative stress markers and muscle damage markers measured before and after 90 day supplementation. Fasting: 10h, then pre-exercise blood samples taken. Standardized breakfast: ~650 kcal Exercise: 2 hours soccer bout Post-exercise: blood samples collected after 15min of rest muscle damage markers, plasma creatine kinase (CK) and aspartate aminotransferase (AST) increased after 2hr exercise. Post-exercise peak CK and AST levels were significantly lower in astaxanthin treated group compared to placebo group. AstaReal Astaxanthin reduces muscle damage from exercise. AstaReal Astaxanthin supports recovery from exercise. BaralicI. et al BaralicI. et al mg/day for 90 days 4mg/day for 90 days 40 trained male soccer players; average age, trained male soccer players; average age 18 Measured saliva and blood markers before and after 90 days of training and supplementation. Measured blood levels of sulfhyryl antioxidant, Paraoxonase1 (PON1), at 0, 45, and 90 days after start of supplementation. PON1 activity was measured using spectrophotometric assay using substrates paraoxon and diazoxon. placebo group had 57% increase in system proinflammatory marker C-reactive protein (CRP), while astaxanthin group did not. Asxsupplementation had a beneficial effect in improving Pon1 activity toward paraoxon and diazoxon. significant interaction effect of supplementation and training (p<0.05) on Pon1 Activity. AstaReal Astaxanthin reduces muscle damage from exercise. AstaReal Astaxanthin supports recovery from exercise. AstaReal Astaxanthin improves antioxidant status

45 Muscle Performance Body of Evidence for AstaReal Astaxanthin Study Dosage Participants Method Result Claims MiyawakiH. et al Saito M. et al Iwabayashi M. et al mg/day for 10 days 12mg/day for 4 weeks 12mg/day for 8 weeks 20 men 200uL whole blood forced through microchannel Array (7um wide, 30um long) and seconds for 100uL to pass through microchannel was measured 20 healthy volunteers 20 healthy postmenopausa l women Laser speckle flowgraphy to measure square blur rate in macular. Increased square blur rate means increased blood flow. Ankle Brachial pressure index (ABI) comparing blood pressure at ankle to blood pressure measured at the arm to assess peripheral blood flow. 10% increase in whole blood transit time in astaxanthin group after 10 days. 12% faster transit time in astaxanthin group compared to placebo group after 10 days. 10.3% increase in macular blood flow at 2 weeks 15.3% increase in macular blood flow at 4 weeks 3.7% increase in ABI after 8 weeks of treatment AstaReal Astaxanthin improves blood flow AstaReal Astaxanthin improves blood flow AstaReal Astaxanthin improves blood flow

46 Muscle Performance Body of Evidence for AstaReal Astaxanthin Study Dosage Participants Method Result Claims Katagiri M. et al mg/day for 12 weeks 29 healthy adults aged CogHealth battery measures response time and accuracy with 5 card games played on a personal computer. The card games include tests of simple reaction, choice reaction, working memory, delayed recall, and divided attention. The response time is measured with a sensitivity of 1/1,000 s. After 12 weeks, astaxanthin group showed faster (6-8%) mean response time (reaction time) in tasks engaging choice reaction*, working memory**, delayed recall* and divided attention* (* = p<0.1, **= p<0.05). Similarly, accuracy (performance) improved in delayed recall (episodic memory) task (p<0.05) AstaReal Astaxanthin improves choice reaction time and spatial attention HongoN. et al mg/day for 8 weeks 39 healthy adults Serial addition tasks for 2 x 15 min, followed by 30 min of cycling, and 60 min of rest After 8 weeks, astaxanthin group reported significant improvement (performance) in sensation of fatigue, mental fatigue, clarity of thinking, concentration, motivation, mood, and body heaviness in response to both mental and physical load. Similarly, astaxanthin group reported significant improvement (recovery) in sensation of fatigue, mental fatigue, physical fatigue, clarity of thinking, concentration, irritation and body heaviness after 60min recovery period following mental and physical load. Astaxanthin group also maintained accuracy scores throughout mental load (calculation exercise), while control group made more errors due to mental fatigue. AstaReal Astaxanthin supports resilience against mental and physical fatigue. AstaReal Astaxanthin support mental clarity.

47 Muscle Performance Body of Evidence for AstaReal Astaxanthin Study Dosage Participants Method Result Claims AoiW. et al % w/w for 4 weeks 32 mice: Rested control group (8 mice), Exercise training group (8 mice), Rested astaxanthin group (8 mice), astaxanthin plus exercise training group (8 mice) Experiment 1: Training at 18m/min for 5min, 2 days/week. After 4 weeks, treadmill exercise assessment, warm up to 30m/min over 5min then measure running time to exhaustion. Experiment 2: Treadmill exercise training 5 times/week for 4 weeks. Intensity and duration of exercise gradually increased to 30m/min for 60min. Experiment 1: Astaxanthin mice had more endurance, averaging ~67min to exhaustion, while control group averaged ~50min to exhaustion (p<0.05). lower plasma lactate levels in astaxanthin group (3.5mM) after 30min compared to control (4.3mM) (p<0.05) Carnitine palmitoyltransferase I (CPT I) fatty acid transported is preferentially recruited to mitochondria in astaxanthin treated mice compared to control mice. CPT I represent rate-limiting step in fat utilization in the muscle. Experiment 2: 20min into the 60min exercise, astaxanthin treated mice had significantly lower respiratory exchange ratio (RER; indicator of muscle oxidative capacity to get energy) than control group(p<0.05). Astaxanthin group had significantly higher lipid oxidation levels (~24mg/kg/min) compared to control (~20mg/kg/min) (p<0.05) Astaxanthin group had significantly lower levels of carbohydrate utilization (~70mg/kg/min) compared to control group (~80mg/kg/min) (p<0.05) AstaReal Astaxanthin supports sustained energy