Figure S1: Abundance of Fe related proteins in oceanic Synechococcus sp. strain WH8102 in. Ferredoxin. Ferredoxin. Fe ABC transporter

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Leon Sparks
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0.004 SW nm 0.04 0.03 nm nm 0.16 0.1 nm nm 0.40 0.3 nm nm 0.80 0.5 nm 1.6 1 nm 16 10 nm 160 100 nm 0 nm 0.04 nm 0.16 nm 0.40 nm 0.80 nm 1.

1 0.004 SW nm nm nm nm nm nm nm nm nm nm nm 0 nm 0.04 nm 0.16 nm 0.40 nm 0.80 nm 1.6 nm 16 nm 160 nm Spectral counts 0 nm 0.04 nm 0.16 nm 0.40 nm 0.80 nm 1.6 nm 16 nm 160 nm Supplemental Information Figure S1: Abundance of Fe related s in oceanic Synechococcus sp. strain WH8102 in response to [Fe'] (SYNW1768) (SYNW0535) Fe(II) ABC transporter, IdiA (SYNW1797) Fe ABC transporter 5 6 1

2 fluorescence fluorescence fluorescence 7 8 Figure S2: Comparison of flow cytometry and fluorescence measurements on the final day of each experiment. 1.E E+07 1.E+06 cellc/ml 1.E+00 1.E+05 fluorescence cells/ml 1.E E+07 1.E+05 cellc/ml 1.E+00 1.E+03 1.E E E+00 1.E+05 1.E+06 1.E+07 1.E+08 cells/ml 10 : 2

3 Table S1: Fe related s in the Synechococcus WH8020, WH8102, and CC9311 genomes. Proteins listed in each row represent the closest matching amino acid sequences among the three strains. Black, green, and blue text indicates that the in a given row is present in three, two, or one of the strain genomes respectively. This information is shown graphically in the Venn diagram in figure 3B. WH8102 WH8020 CC9311 ID Protein ID Protein ID Protein SYNW1841 Cytochrome b6/f complex subunit Cytochrome b6-f complex iron- sync_2149 cytochrome b6-f complex iron- (Rieske iron-sulfur sulfur subunit PetC1 (Rieske iron sulfur subunit SYNW1632 hypothetical WB44_07765 sulfur EC ) Possible 4Fe-4S iron sulfur cluster sync_0748 4Fe-4S iron sulfur cluster binding binding WB44_00870 SYNW1705 hypothetical sync_0892 ferredoxin WB44_01950 SYNW1277 ferredoxin sync_1397 iron-sulfur cluster-binding SYNW1768 SYNW0624 SYNW1981 SYNW0697 SYNW1343 SYNW1274 ferredoxin possible 3Fe-4S ferredoxin ferredoxin putative ferredoxin like ferredoxin WB44_07000 WB44_08620 WB44_13840 WB44_02125 WB44_02385 WB44_04140 WB44_06700 sync_2017 iron-sulfur cluster-binding sync_2348 ferredoxin (3Fe-4S) sync_0535 ferredoxin (2Fe-2S), 2Fe-2S sync_0919 ferredoxin soluble [2Fe-2S] ferredoxin sync_0980 ferredoxin soluble [2Fe-2S] ferredoxin sync_1394 ferredoxin (2Fe-2S) soluble [2Fe-2S] ferredoxin sync_1953 ferredoxin 3

4 SYNW0535 SYNW0497 SYNW0521 putative ferredoxin-thioredoxin WB44_08230 WB44_08440 WB44_10810 soluble [2Fe-2S] ferredoxin sync_2254 ferredoxin soluble [2Fe-2S] ferredoxin sync_2310 ferredoxin, 2Fe-2S soluble [2Fe-2S] ferredoxin sync_2841 ferredoxin -thioredoxin reductase, sync_2274 ferredoxin-thioredoxin reductase SYNW0318 reductase, variable chain thioredoxin reductase, WB44_08310 variable chain variable chain sync_2484 ferredoxin thioredoxin reductase, SYNW0751 catalytic beta chain ferredoxin--nadp reductase WB44_ NADP(+) reductase (EC sync_1003 catalytic beta chain ferredoxin--nadp reductase SYNW2132 -dependent glutamate WB44_ ) -dependent glutamate sync_0387 ferredoxin-dependent glutamate SYNW2477 synthase --nitrite reductase WB44_13115 synthase (EC ) --nitrite reductase (EC sync_2898 synthase ferredoxin-nitrite reductase SYNW1095 -sulfite reductase WB44_ ) --sulfite reductase (EC sync_1280 sulfite reductase subunit beta SYNW1797 putative iron ABC transporter, WB44_03570 WB44_ ) reductase Ferric iron ABC transporter, iron- sync_1545 ABC-type Fe3+ transport system SYNW1798 substrate binding putative iron ABC transporter WB44_04835 binding Ferric iron ABC transporter, sync_2048 periplasmic component ABC-type Fe3+ transport system SYNW1544 ABC transporter, ATP binding WB44_07150 permease ABC transporter, ATP binding sync_1942 permease component ABC transporter ATP-binding component, possibly iron component, possibly iron transporter WB44_06655 transporter 4

5 sync_0682 sync_0681 sync_2395 ferrous iron transport A ferrous iron transport B iron ABC transporter ATP-binding SYNW1340 ABC transporter, ATP binding ABC transporter, ATP binding sync_1757 iron chelate ABC transporter ATP- SYNW2366 domain, possibly Mn transporter conserved hypothetical putative WB44_05820 domain, possibly Mn transporter Mn2+ and Fe2+ transporter, NRAMP sync_2773 binding Mn2+/Fe2+ transporter integralmembrane WB44_10525 WB44_04810 family Ferritin sync_1539 Ferritin sync_0687 Ferritin sync_0854 Ferritin sync_1077 Ferritin sync_0680 Ferritin SYNW1747 ferrochelatase Ferrochelatase, protoheme ferro- sync_2000 ferrochelatase SYNW0865 Ferric uptake regulator family WB44_06915 lyase (EC ) Ferric uptake regulation FUR sync_1167 transcriptional regulator, Fur family SYNW0774 Ferric uptake regulation WB44_03040 Peroxide stress regulator; Ferric sync_1722 transcriptional regulator, Fur family uptake regulation ; Fe2+/Zn2+ uptake regulation SYNW2401 Ferric uptake regulator family WB44_05660 s Ferric uptake regulation sync_2831 transcriptional regulator, Fur family WB44_

6 SYNW2368 putative flavo WB44_06685 Flavodoxin 1 sync_1950 flavodoxin FldA Diflavin flavo SYNW2368 sync_2780 flavodoxin:flavin reductase- SYNW0163 putative glycolate oxidase subunit WB44_10555 Glycolate dehydrogenase (EC sync_0213 likedomain-containing Fe-S oxidoreductase (Fe-S) WB44_ ), iron-sulfur subunit GlcF sync_2669 Iron-containing alcohol dehydrogenase Iron-regulated A precursor sync_1502 Iron-regulated A precursor, SYNW0321 ABC transporter, membrane WB44_04570 Iron-sulfur cluster assembly sync_2481 putative FeS assembly SufD SYNW0320 component ABC transporter, ATP-binding WB44_09210 SufD Iron-sulfur cluster assembly ATPase sync_2482 FeS assembly ATPase SufC SYNW0319 component ABC transporter, membrane WB44_09215 SufC Iron-sulfur cluster assembly sync_2483 cysteine desulfurase activator SYNW0317 component ArsR family transcriptional regulator WB44_09220 SufB complex subunit SufB Iron-sulfur cluster regulator SufR sync_2485 iron-sulfur cluster biosynthesis SYNW0712 possible sufe WB44_09230 Sulfur acceptor SufE for sync_0942 transcriptional regulator SufR Fe-S metabolism, SufE SYNW0007 hypothetical WB44_02220 iron-sulfur cluster assembly family FIG : hypothetical sync_0007 iron-sulfur cluster binding, SYNW2479 ABC transporter component, WB44_11325 manganese/zinc/iron chelate ABC sync_1497 putative manganese/zinc/iron chelate ABC possibly Zn WB44_04545 transporter (MZT) family, permease transporter permease 6

7 SYNW2480 ABC transporter,atp binding ABC transporter,atp binding sync_1498 manganese/zinc/iron chelate ABC SYNW2214 component, possibly zinc transport hypothetical WB44_04550 component, possibly zinc transport probable iron binding from sync_2569 transporter ATP-binding iron-sulfur cluster assembly SYNW1990 hypothetical WB44_09615 the HesB_IscA_SufA family Putative heme iron utilization sync_0526 accessory hypothetical WB44_13800 Non-specific DNA-binding sync_2856 hypothetical Dps / Iron-binding ferritin-like WB44_10870 antioxidant / Ferroxidase 7

8 Table S2: Stoichiometric calculations of iron responsive s in WH8020. The quantitation is based on the average of all measurements for each peptide standard. "Low Fe" values are averages of all measurements 1nM Fe', while "high Fe" values are averages of all measurements >1nM Fe'. The molecular weight and number of N atoms per are based on amino acid sequences for each. Difference Difference in fmol in fg Target Target between between Difference "high" and "high" and in percent abundance abundance "low" "low" between N content N content Protein N atoms "Low Fe" "High Fe" treatments treatments "high" and "Low Fe" "High Fe" molecular per (fmol / g (fmol / g (fmol / g (fg / g "low" (pmol N/ (pmol N/ Peptide standard target weight (fmol N / total total total total treatments* ug total ug total Protein sequences (fg / fmol) fmol) ) ) ) ) (%) ) ) IdiA ADVIILVDAAR, (1196) Ferritin (1188) LVEASGISLVQR, GLTSNVSQPYFGGDIGLIR SQSNDLQALDAPR, FANGDPTALLVIDNELR

9 Flavodoxin (1628) (2042) DLLPGTETK, VPTDGYTFAESK SGAVEQPDAMGVK, ADGFTLLCVAFPCSDLR * Difference in percent is calculated as the difference in under high and low Fe conditions (in g) divided by the total (in g) multiplied by

10 Table S3: Nutrient data from GEOTRACES cruise GT11. For samples collected via geofish, dissolved nitrate and phosphate data were selected based on the nearest sampling station to the dissolved iron sample site (less than 0.2 degrees latitude difference). Station number Collection method latitude (degrees) longitude (degrees) nitrate (nm) phosphate (nm) dissolved Fe (nm) 1 Go-Flo, 30 m o N o W Go-Flo, 35 m o N o W Go-Flo, 28 m o N o W Go-Flo, 41 m o N o W Go-Flo, 32 m o N o W Go-Flo, 40 m o N o W Go-Flo, 41 m o N o W Go-Flo, 41 m o N o W Go-Flo, 41 m o N o W Go-Flo, 40 m o N o W Go-Flo, 41 m o N o W Go-Flo, 51 m o N o W Go-Flo, 40 m o N o W Geofish, mixed layer o N o W Geofish, mixed layer o N o W Geofish, mixed layer o N o W Geofish, mixed layer o N o W Geofish, mixed layer o N o W Geofish, mixed layer o N o W Geofish, mixed layer o N o W Geofish, mixed layer o N o W Geofish, mixed layer o N o W Geofish, mixed layer o N o W

11 Table S4: Parallel reaction monitoring conditions for targeted natural abundance peptides and isotopically labeled peptide standards. Isotopically- Natural labeled Peptide standard target abundance standard Protein sequences mass (m/z) mass (m/z) IdiA ADVIILVDAAR, (1196) LVEASGISLVQR, GLTSNVSQPYFGGDIGLIR Ferritin SQSNDLQALDAPR, (1188) FANGDPTALLVIDNELR Flavodoxin DLLPGTETK, (1628) VPTDGYTFAESK SGAVEQPDAMGVK, (2042) ADGFTLLCVAFPCSDLR

12 Table S5: global proteome data (see excel spreadsheet uploaded separately). 12

Table S9A: List of taurine regulated genes in Bp K96243 Chr 1 (up regulated >=2 fold) Cluster no GENE ID Start Stop Strand Function

Table S9A: List of taurine regulated genes in Bp K96243 Chr 1 (up regulated >=2 fold) Cluster no GENE ID Start Stop Strand Function 1 BPSL0024 26223 26621 + LrgA family BPSL0025 26690 27412 + hypothetical