Liposomal Delivery: the LD Advantage
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1 Liposomal Delivery: the LD Advantage Why this is so important for colostrum effectiveness When a baby is born, the mother s breast does not immediately begin producing milk. Instead it produces a yellowish fluid called colostrum, or foremilk. Colostrum is a highly nutritious substance with extraordinary health-giving qualities. It contains an amazing array of beneficial substances, such as Immunoglobulins (natural antibodies) which offer the infant increased protection from pathogens in its environment, modulators of the immune system to help the infant adapt to challenges, growth factors to complete the development of the gastrointestinal tract, and many other proteins, peptides, and cells that act in concert to protect the infant and prepare it for life in this strange new world it has been thrust into. Colostrum, like milk, is produced by cells in the mammary gland, and like milk, it is expressed from the mammary cells. One of the components of colostrum, cell membrane fats or lipids, are secreted in tiny globules surrounded by a portion of the cell membrane from the mammary cell. This process is shown in Figure 1 which shows how a single secretory cell produces milk (or colostrum) fat globules by pinching off a globule from the cell membrane. The components the cell uses to produce the milk fat are taken up by the cell into small vesicles, which then are processed through the organelles of the cell, the Golgi apparatus and the endoplasmic reticulum, to produce the fat globule. The mitochondria provide the energy that drives the process, while the nucleus of the cell contains the DNA that codes all the proteins the cell produces in its organelles. The membrane surrounding the globule acts to protect the milk fat and colostrum molecules from the digestive action of the gastrointestinal tract until it reaches the intestine where it has the most benefit to the infant.
2 Figure 1. Expression of milk globule by mammary secretory cell. Commercially prepared colostrum powder has this protective membrane removed because the colostrum molecule is shattered and removed in the defatting, pasteurization, drying and/or freezing processes to produce powdered colostrum. In order to protect colostrum from human (post infancy) stomach acids and digestive enzymes, it is necessary to add back the phospholipid coating that was removed and to create phospholipid liposomes around the colostral components. This helps protect the colostrum as it passes through the stomach and delivers it to the small intestine where they can be of most benefit. Liposomes can also penetrate skin surfaces and carry colostrum components into the blood stream for further delivery throughout the body to organs and cells for use as needed. Liposomes also help prevent oxidation of the product between the time of manufacture and its use. Studies have shown that colostrum without Liposomal Delivery (LD) tends to clump together in the gastrointestinal tract, while colostrum with LD readily disperses for maximum absorption and effectiveness. 1 Digestion studies comparing colostrum without LD to colostrum with LD show that Liposomal Delivery (LD) offers nearly 100% protection to IgG (immunoglobulin G) in the colostrum from being broken down by stomach acids and digestive enzymes; compared to almost a 50% destruction of immunoglobulin in colostrum without LD TM. 2 Chemical studies have shown that the phospholipids in LD TM form liposomes, tiny spheres of lipid which enclose and protect the contents, in this case colostrum. 3 In addition to its protective effect, the phospholipids which make up LD TM have important physiologic effects as well. All the cells in your body are protected by a cell membrane made up of the phospholipids found in LD. They play an important part in maintaining cell membrane health and function by providing a
3 lipid sea in which important membrane proteins float. Problems can occur when these phospholipids are not present in the proper ratios. In nerve cells, for example, some chronic nervous conditions, such as Alzheimer s disease, senile dementia and Parkinson s disease, show improvement in symptoms following supplementation with phosphatidylserine. 4-6 Down syndrome is also characterized by an imbalance in brain phospholipids. 7 Phospholipids are helpful in counteracting the effects of stress, which in turn can affect the immune system and disrupt normal intestinal functioning Phospholipids also play important roles in cell signaling the way the interior environment of the cell communicates with the extracellular environment. These include growth inhibition, cellular differentiation and programmed cell death As well, phospholipids play crucial roles in maintenance of the barrier function of the skin, 14,15 antimicrobial defenses of the skin, 16 protection of the stomach lining against ulceration, 17,18 preventing Hepatitis C recurrence, 19 blood clotting, and protein assembly. 24 In short, LD provides not only an effective delivery system not available with any other colostrum but provides vital nutrients in its own right.
4 References 1. Johnston, J. Scanning electron microscopic studies of liposomes done at Northern Arizona University in 2000, unpublished. 2. Buhmeyer, J. MS: Digestion studies done at independent laboratory in 2004, unpublished. 3. Aust, D. Chemical analyses of liposomes, 2002, unpublished. 4. Amaducci, L, et al. Use of phosphatidylserine in Alzheimer's disease. Annals of the New York Academy of Science 640: (1991). 5. Crook, TH, et al. Effects of phosphatidylserine in age-associated memory impairment. Neurology 41(5): (1991). 6. Fünfgeld, EW, Nedwidek, P. Neurohomologous Phosphatidylserine in Parkinsonian Patients with Associated Disorders of Cerebral Metabolism. Clinical Trials Journal 24(1):42-61 (1997). 7. Murphy, EJ, et al. Phospholipid composition and levels are altered in Down syndrome brain. Brain Research 867(1-2):9-18 (2000). 8. Monteleone, P, et al. Blunting by chronic phosphatidylserine administration of the stress-induced activation of the hypothalamo-pituitary-adrenal axis in healthy men. European Journal of Clinical Pharmacology 42(4): (1992). 9. Kelly, GS. Nutritional and botanical interventions to assist with the adaptation to stress. Alternative Medicine Review 4(4): (1999). 10. Benton, D, et al. The influence of phosphatidylserine supplementation on mood and heart rate when faced with an acute stressor. Nutritional Neuroscience 4(3): (2001). 11. Hannun, YA, Linardic, CM. Sphingolipid breakdown products: anti-proliferative and tumor-suppressor lipids. Biochimia et Biophysica Acta 1154(3-4): (1993). 12. Obeid, LM, et al. Programmed cell death induced by ceramide. Science 259(5102): (1993). 13. Parodi, PW. Cows' milk fat components as potential anticarcinogenic agents. Journal of Nutrition 127(6): (1997). 14. Man, M-Q, et al. Optimization of physiological lipid mixtures for barrier repair. Journal of Investigative Dermatology 106(5): (1996). 15. Madison, KC. Barrier function of the skin: "la raison d'etre" of the epidermis. Journal of Investigative Dermatology 121(2): (2003). 16. Bibel, DJ, et al. Sphingosines: antimicrobial barriers of the skin. Acta Dermato-Venereologica 73(6): (1993). 17. Dial, EJ, Lichtenberger, LM. A role for milk phospholipids in protection against gastric acid. Studies in adult and suckling rats. Gastroenterology 87(2): (1984). 18. Kivinen, A, et al. Gastroprotective effect of milk phospholipids, butter serum lipids and butter serum on ethanol and acetylsalicylic acid induced ulcers in rats. Milchwissenschaft 1991: (1991). 19. Niederau, C, et al. Polyunsaturated phosphatidyl-choline and interferon alpha for treatment of chronic hepatitis B and C: a multi-center, randomized, double-blind, placebo-controlled trial. Leich Study Group. Hepatogastroenterology 45(21): (1998). 20. Smirnov, MD, et al. The effect of membrane composition on the hemostatic balance. Biochemistry 38(12): (1999).
5 21. Safa, O, et al. Lipid oxidation enhances the function of activated protein C. Journal of Biological Chemistry 276(3): (2001). 22. Griffin, JH, et al. Plasma lipoproteins, hemostasis and thrombosis. Thrombosis and Haemostasis (86(1): (2001). 23. Majumder, R, et al. Soluble phosphatidylserine triggers assembly in solution of a prothrombinactivating complex in the absence of a membrane surface. Journal of Biological Chemistry 277(33): (2002). 24. Wang, X, et al. Topology of polytopic membrane protein subdomains is dictated by membrane phospholipid composition. EMBO Journal 21(21): (2002). This information is copyrighted and may not be reproduced without express permission from the Center for Nutritional Research.
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